• The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
  • During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
  • The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
  • A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • This clinical trial uses a laboratory test called a high throughput sensitivity assay in planning treatment for patients with relapsed or refractory acute myeloid leukemia. (clincosm.com)
  • To obtain results from a high throughput drug sensitivity assay within 10 days, procure drug within 14 days and initiate treatment within 21 days. (clincosm.com)
  • A patient receives a drug intervention based on the results of a high throughput sensitivity assay. (clincosm.com)
  • Patients receive 1 of 160 possible interventions based on high throughput drug sensitivity assay. (clincosm.com)
  • Whether treatment was administered in the time frame based on the high throughput drug screen. (clincosm.com)
  • This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
  • We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
  • Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
  • In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)
  • FDA approved drugs not currently used to treat ependymoma were also identified that posses selective toxicity against ependymoma cells relative to normal NSCs both in vitro and in vivo, e.g., 5-fluorouracil. (nih.gov)
  • In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
  • The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
  • In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
  • While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
  • Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
  • Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
  • H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
  • The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • The Drug Repurposing Hub is one of the most comprehensive and up-to-date biologically annotated collections of FDA-approved compounds in the world. (broadinstitute.org)
  • Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
  • The inclusion of other assays and compounds will most likely expand the types of activities that can be reliably detected. (holocausto.online)
  • Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)
  • Methods The literature related to PMS were collected from China Knowledge Network (CNKI), Wanfang database and Weipu database in the past 20 years, the herbal compound prescriptions for the treatment of PMS were screened and a relevant database were established and analyzed by SPSS. (bvsalud.org)
  • Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. (ucdenver.edu)
  • Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. (broadinstitute.org)
  • Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. (broadinstitute.org)
  • The aim is to try to identify drugs that may be effective in killing leukemia cells for those patients who will not be cured with conventional chemotherapy. (clincosm.com)
  • NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
  • Immunosuppressive agents encompassed a large number of drugs, mainly represented by anti-tumour necrosis factor (TNF)-α, anti-retroviral therapy (ARV), azathioprine (AZT), methotrexate (MTX), mycophenolate mofetil (MMF), non-steroidal anti-inflammatory drugs (NSAIDs), rituximab and steroids. (ersjournals.com)
  • Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
  • The goal is to use this laboratory assay to best match a drug to a patient's disease. (clincosm.com)
  • This assay best matches a drug to the patient's disease. (clincosm.com)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma. (broadinstitute.org)
  • Results The relevant literatures contains 184 Chinese medicine prescriptions/proprietary Chinese medicines, 122 flavors of traditional Chinese medicines, and the drug categories were mainly tonic drugs, antipyretic drugs, astringent drugs, and tranquilizers. (bvsalud.org)
  • antigens, methods, and interpretation criteria for these assays differ from those for IGRAs approved previously by FDA. (cdc.gov)
  • An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • Otherwise, patients receiving at least one immunosuppressive drug (especially steroids) are more likely to have an indeterminate result [ 1 , 4 , 7 , 8 ]. (ersjournals.com)
  • We made the hypothesis that the proportion of "bad" responders to mitogen among patients with negative QFT could be underestimated, especially during immunosuppressive drug intake. (ersjournals.com)
  • Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • This graph shows the total number of publications written about "Drug Screening Assays, Antitumor" by people in this website by year, and whether "Drug Screening Assays, Antitumor" was a major or minor topic of these publications. (ucdenver.edu)
  • As urinary screens, they are noninvasve and apble on a large scale with current labo techniques For short-term esue, glucaric acid, thloetbers, and mug tests are usefl. (cdc.gov)
  • We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
  • Functional T-cell assays are exposed to bias at several levels (manufacturing, pre-analytical, analytical and immunological) [ 2 ] and, unfortunately, the lack of a gold standard precludes higher diagnostic accuracy. (ersjournals.com)
  • We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)
  • This assay will test multiple drugs simultaneously against a patient's own donated blood sample. (clincosm.com)
  • Time from sample procurement to assay results. (clincosm.com)
  • We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)
  • Extraction and direct contact assays were performed using human epidermal keratinocytes and mouse fibroblasts and mouse epithelial cells. (holocausto.online)
  • Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)