• After several days, lymphocytes underwent blast transformation, DNA synthesis and cellular proliferation in response to the major histocompatibility antigen (MHC Class I and II) differences between the two cell populations designated as Responder and Stimulator cells. (wikipedia.org)
  • Human alveolar macrophages from bronchoalveolar lavage are usually poor accessory cells for antigen-induced T-lymphocyte proliferation and poor stimulators of allogeneic mixed leukocyte reactions (MLR) when compared to peripheral blood monocytes. (elsevierpure.com)
  • PTX and several cogeners were found to directly suppress T cell proliferation in response to phytohemagglutinin, to allogeneic cells in a mixed leukocyte reaction, and to cross-linking the CD3 complex. (elsevierpure.com)
  • After the co-culture of B16 cells and CD8 + T cells, the effects of Notch1 on the proliferation and activation of T cells were assessed by CCK8 assay, CFSE dilution and Chromium-release test. (biomedcentral.com)
  • We showed higher DTH, increased lymphocyte proliferation, decreased tumor growth and reduced JAK2/STAT3 phosphorylation in mice treated with naringenin and CPT. (biomedcentral.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • 1964. Lymphocyte interaction: A potential histocompatibility test in vitro. (wikipedia.org)
  • Methods We used several in vitro methods including Caco-2/THP-1 leaky gut model, AhR activation, PBMC assay and mixed lymphocyte reaction (MLR) to assess the impact of a MET-C candidate (MaaT03X) on gut homeostasis and immune activation. (bmj.com)
  • We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)
  • There is experimental evidence from in vitro studies of animal cells that some cannabinoids, including THC, can produce a variety of changes in cellular processes in vitro (i.e. in the test tube). (druglibrary.org)
  • The fundamental of hybridoma technology is based on the immortalization of B lymphocyte cells having antibody producing capacity but limited growth characteristics in vitro. (savedelicious.com)
  • Mixed lymphocyte reaction (MLR) is a test used by pharmaceutical and biotech organizations to show the safety of a drug or implantable material. (wikipedia.org)
  • Put simply, it is mixing populations of T-lymphocytes (category of white blood cells) together, and measuring the reaction that occurs. (wikipedia.org)
  • Stimulator cell populations that also contain T-cells (Two way mixed lymphocyte reaction) will replicate in the presence of the Responder cells, therefore for a One way mixed lymphocyte reaction, stimulator cells are prevented from replicating by irradiation or treatment with mitomycin C, a DNA crosslinker to prevent cell replication. (wikipedia.org)
  • 1963. A reaction between leukocytes in mixed peripheral blood cultures. (wikipedia.org)
  • Urine cultures showed reaction (RT-PCR) in specimens from the same patient, we penicillin-sensitive enterococci, and blood cultures were nega- were able to compare the entire genomic sequence of the tive. (cdc.gov)
  • 1984. Depression in B- and T-lymphocyte mitogen- induced blastogenesis in mice exposed to low concentrations of benzene. (cdc.gov)
  • No MLR occurred when the Responder cells came from thymectomized animals, whereas bursectomized chicken leukocytes reacted in culture demonstrating that T-cells were the major cell type in Responder cell populations. (wikipedia.org)
  • Loosely adherent cells (LAM) were obtained after an overnight incubation on plastic culture dishes of initially adherent mononuclear cells. (elsevierpure.com)
  • A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
  • Subsequent characterization of this virus has shown that it is not significantly related to any known animal retrovirus, is not an endogenous (genetically transmitted) virus of man, and so far has been associated only with fresh or cultured T -cells from patients with T -cell neoplasia. (science-connections.com)
  • 1975) have required the establishment of continuously growing cell lines from the disease of origin or cocultivation of these cells with previously established cell lines, the ability to grow malignant T -cells in long-term culture could facilitate the isolation of retroviruses from these diseased states. (science-connections.com)
  • The disease may become more chronic and the originally pure neutrophilic inflammation may then become a mixed inflammatory cell inflammation with neutrophils, plasma cells and lymphocytes. (vin.com)
  • These lymphocytes may also be present in the lumen and epithelial cells of the bile ductules. (vin.com)
  • Number of independent experiments : At least 2 cultures were prepared per test group (referred to as A and B), and at least 1000 cells per culture were evaluated for the occurrence of micronucleated cells. (europa.eu)
  • Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. (nature.com)
  • The aim of our research was to determine how immunotherapy changes the proportion of lymphocyte subsets in dog peripheral blood and the levels of cytokines secreted by these cells during therapy. (mdpi.com)
  • Notch1 expression in B16 melanoma cells inhibited the infiltration of CD8+ cytotoxic T lymphocytes and NK cells and reduced IFN-γ release in tumor tissue. (biomedcentral.com)
  • The key cells of the immune system are the white blood cells known as lymphocytes. (britannica.com)
  • T lymphocytes ( T cells ) and B lymphocytes ( B cells ). (britannica.com)
  • T lymphocytes are responsible for cell-mediated immunity , so named because the T cells themselves latch onto the antigens of the invader and then initiate reactions that lead to the destruction of the nonself matter. (britannica.com)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • EBV-transformed B cells (EBV-LCL) were cultured in IMDM (Cambrex, Verviers, Belgium) with 10% Fetal Bovine Serum (FBS). (haematologica.org)
  • For fifteen years, he conducted research into fuel cells with the Material Sciences and Engineering department of Delft University of Technology. (clubofamsterdam.com)
  • All the tested huprines are moderately potent and selective trypanocidal agents, exhibiting IC50 values against T. brucei in the submicromolar to low micromolar range and selectivity indices for T. brucei over L6 cells of approximately 15, thus constituting interesting trypanocidal lead compounds. (ndltd.org)
  • This study tested the hypothesis that double overexpression of miR-19a & miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left-ventricular-ejection-fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat. (researchsquare.com)
  • The lymphocytes are fused with cells from a non-antibody producing and myeloma (continuously growing tumor cell line), as the hybrids continue to secrete antibodies while gaining the immortality of the parent tumor cells. (savedelicious.com)
  • Cell fusion to produce hybridomas: The washed lymphocytes are mixed with myeloma cells in an appropriate medium which then exposed to PEG (polyethylene glycol) for a period of time and fusion takes place (Biology Discussion, 2018). (savedelicious.com)
  • When incubation is finished, the viable cells are our hybridomas and these cells are again transferred to the normal culture medium. (savedelicious.com)
  • The protocol of Dr. Collins on the web site also discusses the use of lymphocytes for cells and/or UV treatment of these. (neb.com)
  • Treat cultured mouse fibroblast (NIH 3T3) or osteocyte cells with trypsin to detach the cells from the dish. (neb.com)
  • Diagnosis is by detecting lymphopenia, absence or a very low number of T cells, and impaired lymphocyte proliferative responses to mitogens. (msdmanuals.com)
  • Several whole blood samples and one serum sample underwent further testing. (cdc.gov)
  • MaaT Pharma, a clinical-stage biotech pioneer in the development of Microbiome Ecosystem Therapies TM (MET) in oncology, has developed a unique, ground-breaking, patented pooled co-culture process (MET-C). This technology allows to replicate and leverage, at large industrial scale, the diversity of native-based microbiome ecosystems while tuning the resulting product according to indication-specific compositions. (bmj.com)
  • 3 Autologous lymphocytes genetically modified with the TCR for MART-1/Melan-A have been adoptively transferred to patients with advanced melanoma. (haematologica.org)
  • Immunotherapy engages the immune system in the battle against cancer by taking advantage of the capacity of the T lymphocyte for antigen-directed cytotoxicity [ 14 ]. (biomedcentral.com)
  • Kato Katz thick smears and circulating cathodic antigen tests were performed for S. mansoni screening, whereas thick and thin blood smear techniques were performed for P. falciparum screening. (scirp.org)
  • It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs. (lookformedical.com)
  • Clinicopathological tests showed hypoxia and systemic inflammation. (scielo.org.za)
  • Bacterial may also be found cytologically, but culture is needed to identify and specify the infection, and to allow sensitivity testing. (vin.com)
  • Neutrophilic cholangitis should be treated with antibiotics, preferably based on sensitivity testing after culture. (vin.com)
  • Depending on the source of specimen, treatment with antibiotics, as indicated for the purpose of determining sensitivity, may be required before inoculation of tissue cultures. (medscape.com)
  • Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
  • No / A series of 19 huprines has been evaluated for their activity against cultured bloodstream forms of Trypanosoma brucei and Plasmodium falciparum. (ndltd.org)
  • Lymphocytic cholangitis is characterized by infiltration of the portal tracts with small lymphocytes. (vin.com)
  • Third, if cannabinoids have immunosuppressive effects, then this would have mixed implications for their therapeutic use. (druglibrary.org)
  • Vaccination of BALB/c mice with DREP-GP, DREP-VP40, or DREP-GP+DREP-VP40 vectors, followed by immediate electroporation resulted in a mixed IgG subclass production, which recognized EBOV GP and/or VP40 proteins. (frontiersin.org)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • If a large amount of cellular material is seen in the transport medium (cloudy appearance), the specimen is centrifuged for 5 minutes at full speed and the supernatant used for tissue culture inoculation. (medscape.com)
  • In 1975, Kohler and Milstein gave a concept of "one lymphocyte-one antibody" in the form of hybridoma (Kumar et al. (savedelicious.com)
  • 2018). Each lymphocyte produces only one type of antibody for a specific antigenic epitopes and termed as monoclonal antibody. (savedelicious.com)
  • This technology for monoclonal antibody production opens a vast area of application specifically in medical sciences (Kumar et al. (savedelicious.com)
  • The aliquots are distributed to 96-well plastic culture plates to test periodically for expected antibody reactivity (Kulkarni, 2002). (savedelicious.com)
  • From January 2002 to August 2015, 62 patients with Brucella-associated spondylitis were treated with comprehensive diagnosis based on epidemiological history, clinical manifestations, imaging findings, laboratory tests, and local biopsy. (scirp.org)
  • A bacteriological culture may be performed using a throat swab, expectorated throat sputum, or throat washings the respiratory tract of patients with pharyngitis, croup, bronchitis, or pneumonia. (medscape.com)
  • Swingle 1974) In the search for new meaningful procedures for the detection and evaluation of anti-inflammatory drugs, the rabbit eye as a test system was studied. (ndltd.org)
  • The cultures are tested for several times by using ELISA or RIA methods. (savedelicious.com)
  • In early tests, the biomechanical treatment removed more than 90 percent of toxins from infected human blood within a few hours. (discovermagazine.com)
  • Researchers then tested their device by cleaning 5 liters of human blood spiked with a variety of pathogens. (discovermagazine.com)
  • The next step for researchers is to test their device in pigs, after which human trials could begin within a couple years, Nature reports. (discovermagazine.com)
  • A scanning electron micrograph of a human T lymphocyte (or T cell) from the immune system of a healthy person. (britannica.com)
  • We investigated the use of human CD20 as suicide gene in T-lymphocytes. (haematologica.org)
  • There is, at most, mixed evidence that THC and other cannabinoids are mutagenic in standard microbial assays, such as the Ames test, and there is contradictory evidence on whether the cannabinoids are clastogenic, i.e. produce breaks in chromosomes. (druglibrary.org)
  • Immunization of the test animals: Test animals are injected a specific immunogen mixed with an adjuvant at several sites of the body and at different times. (savedelicious.com)
  • The evaluation indicators were pain scores, activity of daily living (ADL), imaging findings, and laboratory tests. (scirp.org)
  • This response became quantifiable when incorporating radioactive [3H] labelled thymidine or 5-bromo-2'-deoxyuridine (BrdU), into the mixed cell suspension. (wikipedia.org)
  • Throat culture refers to the growth and isolation of a micro-organism from a specimen taken from the throat. (medscape.com)
  • Culture media include blood agar (BA), chocolate agar (CA), and Mueller-Hinton tellurite blood agar or Tinsdale agar (if Corynebacterium diphtheriae infection is suspected). (medscape.com)
  • Microscopy is of no value for throat swabs unless Ludwig angina (a mixed infection caused by a spirochaeta and a gram-negative anaerobic fusiform) is suspected. (medscape.com)
  • Proteomic screening methods to find new physiopathogenic candidate proteins have not been widely employed in cancer, although a large number of molecular genetic tests have been performed with variable results. (biomedcentral.com)
  • WHO is not responsible, and does not accept any liability, for the testing of pesticides for compliance with the specifications, nor for any methods recommended and/or used for testing compliance. (who.int)
  • When those nanobeads are mixed with infected blood they adhere to pathogens, and then, as the blood passes through channels inside the device, magnets pull out the beads with pathogens attached. (discovermagazine.com)
  • This work has been conducted within TPM as part of the 'Greening of Gas' project, financed by the Ministry of Economic Affairs, the Ministry of Education, Culture and Science and the Ministry of Housing, Spatial Planning and the Environment (VROM) under the Economy, Ecolgy and Technology (EET) programme. (clubofamsterdam.com)