ReceptorsLigandsMacrophagesAntibodyThrombospondinDifferentiationPlateletsProteinAtherosclerosisReceptor class B typeProteinsPlateletBindsFattyApoptotic cellsUptakeExtracellularPlasma membraneModulateAntibodiesIntracellularCellsMiceFoam cell formationAbsenceHumanSpeciesCellMolecularFunctionsSignalRole
Receptors7
- The protein itself belongs to the class B scavenger receptor family which includes receptors for selective cholesteryl ester uptake, scavenger receptor class B type I (SR-BI) and lysosomal integral membrane protein II (LIMP-II). (wikipedia.org)
- CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. (exbio.cz)
- They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. (creative-biolabs.com)
- These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. (biomedcentral.com)
- Through those mechanisms, ox-LDLs increase the inflammatory process, induce monocytes prematurely Ruxolitinib sulfate entering into the subendothelial space and differentiating into macrophages, and up-regulate the manifestation of scavenger receptors (SRs) and toll-like receptors (TLRs) (3, 4) on the surface of triggered macrophages. (rawveronica.com)
- The activity of those cells depends on the generation of a large number of antigen receptors, such as T-cell receptors (TCRs) and immunoglobulin, by somatic rearrangement processes in blast cells. (rawveronica.com)
- Increased foam cell formation results from lack of VDR-SERCA2b interaction, causing SERCA dysfunction, activation of ER stress-CaMKII-JNKp-PPARγ signaling, and induction of the scavenger receptors CD36 and SR-A1. (regenerativemedicine.net)
Ligands2
- This observation led us to hypothesize that CD36 ligands might be generated during vascular injury in the absence of hyperlipidemia and systemic oxidant stress. (techblessing.com)
- For this, we studied the regulation of CD36, CXCL16, LOX-1, SR-A1 and SR-B1 expression on MDDC treated with DEP associated or not with TLR2, 3 and 4 ligands. (biomedcentral.com)
Macrophages7
- CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. (thermofisher.com)
- The antibody TR9 reacts with an extracellular epitope of CD36 (GPIIIb), a 85 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. (exbio.cz)
- Berre S, Gaudin R, Cunha de Alencar B, Desdouits M, Chabaud M, Naffakh N, Rabaza-Gairi M, Gobert FX, Jouve M, Benaroch P: CD36-specific antibodies block release of HIV-1 from infected primary macrophages and its transmission to T cells. (exbio.cz)
- The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. (exbio.cz)
- Macrophages are innate immune cells that internalize and present exogenous antigens to T cells via MHC class II proteins. (bvsalud.org)
- In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. (thermofisher.com)
- By performing proximity labeling and proteomic analysis, we identified scavenger receptor CD36 as a cell surface protein interacting with Siglec-E. Further experiments performed in HEK293T cells transiently overexpressing Siglec-E and CD36 and peritoneal macrophages demonstrated that depletion of cell surface sialic acids by treatment with sialyltransferase inhibitor or sialidase did not affect interaction between Siglec-E and CD36 but retarded Siglec-E-mediated inhibition on oxidized LDL uptake. (biomedcentral.com)
Antibody11
- The following product was used in this experiment: CD36 Monoclonal Antibody (OTI4H7), Biotin, TrueMAB™ from Thermo Fisher Scientific, catalog # TA700031. (thermofisher.com)
- CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. (exbio.cz)
- Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD36 (TR9) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
- Separation of human CD36 positive platelets (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD36 (TR9) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
- Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. (exbio.cz)
- Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). (exbio.cz)
- This product is a HRP-conjugated anti-mouse CD36 monoclonal antibody gernerated from the mouse. (creative-biolabs.com)
- Knockdown Validated: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. (novusbio.com)
- This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- The effector activity of the adaptive immune system includes direct assault of antigen bearing cells by cytotoxic T lymphocytes (CTL), activation to B-cells to produce antibody against the antigen, and induction of swelling, with enhanced innate response, in the area near the antigen. (rawveronica.com)
- dashed line histogram) or APC Anti-Human CD36 antibody (Cat. (bdbiosciences.com)
Thrombospondin1
- However exposure to the thrombospondin ligand causes CD36 to dimerize. (wikipedia.org)
Differentiation1
- CD36 (cluster of differentiation 36), also known as platelet glycoprotein 4, fatty acid translocase (FAT), scavenger receptor class B member 3 (SCARB3), and glycoproteins 88 (GP88), IIIb (GPIIIB), or IV (GPIV) is a protein that in humans is encoded by the CD36 gene. (wikipedia.org)
Platelets4
- Although CD36 was first isolated and structurally characterized from platelets (1), where it was termed glycoprotein IV or IIIb, its functional role on platelets has been obscure. (techblessing.com)
- Recently our group, in collaboration with Podrez, Hazen, and Byzova, showed that atherogenic oxLDL binds and activates platelets in a CD36-dependent manner (28). (techblessing.com)
- Flow cytometric analysis of CD36 expression on human peripheral blood platelets. (bdbiosciences.com)
- The fluorescence histogram showing CD36 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact platelets. (bdbiosciences.com)
Protein5
- The CD36 antigen is an integral membrane protein found on the surface of many cell types in vertebrate animals. (wikipedia.org)
- Using Kyte-Doolittle analysis, the amino acid sequence of CD36 predicts a hydrophobic region near each end of the protein large enough to span cellular membranes. (wikipedia.org)
- It is not clear what role these linkages play on the function of the mature CD36 protein on the cell surface. (wikipedia.org)
- Full length human recombinant protein of human CD36 produced in HEK293T cell. (thermofisher.com)
- they predominantly are CD4+, CD3 +, TCR/ +, T-cells, which identify protein antigens offered to them as fragments bound to major histocompatibility complex class II (MHC-II) molecules. (rawveronica.com)
Atherosclerosis2
- Several in vitro research and tests with Compact disc36C mice exposed a job for Compact disc36 in a variety of important biological processes, including atherosclerosis (17), tumor angiogenesis (18), energy metabolism (19), inflammation (20), host defense (21, 22), antigen presentation (3), and taste (23). (techblessing.com)
- Because normal hemostasis is maintained in the absence of CD36, these studies suggest that CD36 might be an attractive target for antithrombotic therapy in the setting of atherosclerosis. (techblessing.com)
Receptor class B type1
- DESIGN: We investigated the effect of adding an entry inhibitor (the anti-scavenger receptor class B type I mAb1671) to a DAA monotherapy (the protease inhibitor ciluprevir) in human-liver mice chronically infected with HCV of genotype 1b. (itg.be)
Proteins1
- It imports fatty acids inside cells and is a member of the class B scavenger receptor family of cell surface proteins. (wikipedia.org)
Platelet3
- Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7. (thermofisher.com)
- Human platelet CD36 deficiency (known as the NakaC phenotype) is common in Asian and African populations (24C27), and similar to the situation in mice, is not associated with an obvious bleeding diathesis. (techblessing.com)
- The prothrombotic phenotype associated with hyperlipidemia in apoE-null mice fed a Western-type high-fat diet was rescued by genetic deletion of CD36, demonstrating an important link among platelet CD36, hyperlipidemia, oxidant stress, and thrombosis. (techblessing.com)
Binds1
Fatty3
- Work in genetically modified rodents suggest a role for CD36 in fatty acid metabolism, heart disease, taste, and dietary fat processing in the intestine. (wikipedia.org)
- In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. (exbio.cz)
- Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. (exbio.cz)
Apoptotic cells1
- We were particularly interested in the role of microparticles (MPs) in this process, because previous studies from our lab and others demonstrated that CD36-dependent phagocyte reputation and uptake of apoptotic cells and/or shed photoreceptor external sections was mediated by binding of Compact disc36 to phosphatidyl serine (PS) and/or oxPS on the areas (13, 29C31). (techblessing.com)
Uptake2
- VAV, a downstream effector implicated in CD36-mediated oxidized LDL uptake, was shown to interact with SHP-1 following oxidized LDL treatment. (biomedcentral.com)
- Mechanistically, Siglec-E interacts with CD36 to suppress downstream VAV signaling involved in modified LDL uptake. (biomedcentral.com)
Extracellular3
- Based on this notion and the observation that CD36 is found on the surface of cells, CD36 is thought to have a 'hairpin-like' structure with α-helices at the C- and N- termini projecting through the membrane and a larger extracellular loop (Fig. 1). (wikipedia.org)
- Based on the crystal structure of the homologous SCARB2, a model of the extracellular domain of CD36 has been produced. (wikipedia.org)
- Disulfide linkages between 4 of the 6 cysteine residues in the extracellular loop are required for efficient intracellular processing and transport of CD36 to the plasma membrane. (wikipedia.org)
Plasma membrane2
- Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. (exbio.cz)
- van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. (exbio.cz)
Modulate1
- the lipoprotein acts as an agonist to modulate antigen presenting cell functions in response to the pathogen. (creative-biolabs.com)
Antibodies2
- Anti-CD36 antibodies inhibit adhesive functions (e.g. adherence of infected erythrocytes to target cells). (exbio.cz)
- Bertrand G, Renac V, Lefaix MC, Nivet C, Trudel E, Richard L: Neonatal Intracranial Hemorrhage with a Dramatic Outcome Due to Maternal Anti CD36 Antibodies. (exbio.cz)
Intracellular1
- CD36 is modified with 4 palmitoyl chains, 2 on each of the two intracellular domains. (wikipedia.org)
Cells7
- This topology is supported by transfection experiments in cultured cells using deletion mutants of CD36. (wikipedia.org)
- Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
- CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. (exbio.cz)
- Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
- DC are professional antigen-presenting cells that are essential for initiating adaptive immune responses. (biomedcentral.com)
- The cells of adaptive immunity identify specific molecular constructions revealed by antigen showing cells in the contest of MHC determinants. (rawveronica.com)
- Here, following pulmonary immunization with a Salmonella vaccine strain, mouse MAIT cells expanded as separate CD127-Klrg1+ and CD127+Klrg1- antigen-adapted populations that differed in terms of their transcriptome, function and localization in lung tissue. (biomed.news)
Mice3
- In humans, rats and mice, CD36 consists of 472 amino acids with a predicted molecular weight of approximately 53,000 Da. (wikipedia.org)
- Immunofluorescence confocal microscopy of murine carotid thrombi from Compact disc36C mice demonstrated a substantial decrement in endothelial antigen build up, which implies that Compact disc36 is important in MP recruitment into thrombi. (techblessing.com)
- We now report a more general role for CD36 in thrombus formation in normal mice. (techblessing.com)
Foam cell formation1
- These truncated phosphatidylcholine species are likely involved in the CD36-mediated recognition of oxidized lipoproteins and foam cell formation in vivo . (gerli.com)
Absence1
- In the absence of ligand, membrane bound CD36 exists primarily in a monomeric state. (wikipedia.org)
Human2
- Flow cytometry analysis (surface staining) of CD36 in human peripheral blood with anti-CD36 (CB38) purified, GAM-APC. (exbio.cz)
- Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. (exbio.cz)
Species1
- Thus, the species active on CD36 have been identified to have an sn-2 acyl group that incorporates a terminal g -hydroxy (or oxo)- a , b -unsaturated carbonyl (alcohol or aldehyde group). (gerli.com)
Cell1
- Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. (exbio.cz)
Molecular1
- However, CD36 is extensively glycosylated and has an apparent molecular weight of 88,000 Da as determined by SDS polyacrylamide gel electrophoresis. (wikipedia.org)
Functions1
- The function of these lipid modifications is currently unknown but they likely promote the association of CD36 with the membrane and possibly lipid rafts which appear to be important for some CD36 functions. (wikipedia.org)
Signal1
- This dimerization has been proposed to play an important role in CD36 signal transduction. (wikipedia.org)
Role1
- These total results provide what we should believe to be always a novel role for CD36 in thrombosis. (techblessing.com)