MutagenesisDirected Molecular EvolutionMutagenesis, Site-DirectedDigitoxinProtein EngineeringDigoxigeninMolecular Sequence DataModels, MolecularMutagenesis, InsertionalAmino Acid SubstitutionEscherichia coliAmino Acid SequenceMutationSubstrate SpecificityGene LibraryBase SequenceBinding SitesKineticsStructure-Activity RelationshipPlasmidsRecombinant ProteinsCodonCatalytic DomainPoint MutationCatalysisProtein Structure, SecondaryEnzyme StabilityProtein ConformationCloning, MolecularProtein Structure, TertiaryDNA PrimersDNAPolymerase Chain ReactionSaccharomyces cerevisiaeDNA, BacterialOximetryOxygenProtein BindingSequence Homology, Amino AcidDNA Transposable ElementsBacterial ProteinsOxyhemoglobinsCysteineCell LineTransferrinSequence AlignmentGenes, BacterialAlanineMutagensEthylnitrosoureaSpectroscopy, Near-InfraredHemoglobinsIronHydrogen-Ion ConcentrationEscherichia coli ProteinsHistidineDNA Mutational AnalysisTransfectionCrystallography, X-RayConserved SequenceSOS Response (Genetics)Genetic Complementation TestTemperatureAnoxiaPhenotypeRestriction MappingRecombinant Fusion ProteinsBinding, CompetitiveAspartic AcidEthyl MethanesulfonateLysineOligodeoxyribonucleotidesSequence DeletionFerritinsOxygen ConsumptionCricetinaePartial PressureBlood Gas AnalysisAmino Acid MotifsTranscription, GeneticMutant ProteinsPromoter Regions, GeneticMagnetic Resonance SpectroscopyTime FactorsUltraviolet RaysOxygen Inhalation TherapyLigandsCell MembraneGene Expression Regulation, BacterialModels, BiologicalDNA-Binding ProteinsBlood Gas Monitoring, TranscutaneousGenetic VectorsMembrane ProteinsHemochromatosisAsparagineCOS CellsCarbon DioxideElectrophoresis, Polyacrylamide GelSerine