Using default configurations, 100?nt flanking each aspect from the genomic sequences were extracted for prediction of RNA supplementary structure using RNAfold [16]. (clinical-research-informatics.com)
Methylation3
We performed a methylation-sensitive amplification polymorphism analysis to search for targets of RNA-directed DNA methylation in Arabidopsis thaliana and identified several members of a gene family encoding cysteine-rich peptides (CRPs). (biomedcentral.com)
RNA-directed DNA methylation results in a characteristic modification pattern that is typified by methylation of cytosines in all sequence contexts (CG, CHG and CHH, where H is A, T or C) within the region of small RNA-DNA sequence homology [ 4 ]. (biomedcentral.com)
Our review provides insight about the crosstalk among the genetic control of the flowering gene network, methylation (methyltransferases/demethylases) and external signals (cold, light, sRNA and phytohormones) in vernalization and photoperiod pathways. (bvsalud.org)
Annotation1
Following the completion of most annotation steps, sequencing libraries had been employed for size saturation and distribution evaluation. (clinical-research-informatics.com)
Probe1
Each gene of interest is represented by multiple oligonucleotide probe sets. (bio-microarray.com)
Transcriptome3
Our microarray testing platform provides a full range of microarray-based mitochondrial transcriptome analysis tools and testing services to help our global customers conduct relevant research and reduce research costs. (bio-microarray.com)
We have designed a microarray tool for mitochondrial transcriptome analysis. (bio-microarray.com)
CD Genomics provides high-quality microarray tools and testing services for mitochondrial transcriptome analysis and research. (bio-microarray.com)
Genome2
Technology transfer of automated sequencing & software to Genome Therapeutics Corp. resulted in the first commercial genome sequence (the human pathogen, H. pylori, 1994). (codondevices.com)
The human mitochondrial genome is one of the first genomes to be fully sequenced, but the molecular mechanism of mtDNA expression still needs a lot of research. (bio-microarray.com)
Molecular2
His PhD from Harvard in Biochemistry & Molecular Biology with Wally Gilbert included the first direct genomic sequencing method in 1984. (codondevices.com)
He invented the broadly-applied concepts of molecular multiplexing and tags, homologous recombination methods, and array DNA synthesizers. (codondevices.com)
Expression1
Total RNA sequencing from PIWIL1 short hairpin knock-downs and CRISPR-generated knockouts were used for differential gene and transposon expression. (nih.gov)
Genes3
A conserved intergenic region between genes ydaN and dbpA was predicted to encode an sRNA, adjacent to where another non-coding RNA (C0343) has been identified. (wikipedia.org)
This tool can be used for transcription analysis of 37 mitochondrial genes, about 500 genes related to energy metabolism, 300 nuclear genes related to mitochondrial genetic diseases, and more than 1,000 nuclear genes related to nuclear mitochondria. (bio-microarray.com)
Here, we established a sensitive CRISPR/Cas12a array based on Multiple-recombinase polymerase amplification (M-RPA) for CaPV differentiation, which provided a more comprehensive and accurate differentiation mode targeting VARV B22R and RPO30 genes. (bvsalud.org)
Data2
Id of miRNA differential appearance We initial normalized miRNA-sequence data from 18 libraries as transcripts per million (TPM). (clinical-research-informatics.com)
We provide one-stop testing and analysis services, from sample pre-processing, microarray testing to data collection and analysis, you can leave it to us with confidence. (bio-microarray.com)
Differential1
The array regarding proteome modifications brought on by renewal have been assessed among settings (2 l) and 1, A dozen, Twenty-four, Forty eight, and Seventy two postamputation concerning quantitative differential proteomics investigation according to two-dimensional carbamide peroxide gel electrophoresis matrix-assisted lazer desorption/ionization along with differential in-gel electrophoresis Orbitrap evaluation. (kinasepathway.com)
Technology1
We therefore executed a comprehensive analysis using Solexa sequencing technology over the Pekin duck microRNAome over six levels of feather advancement at times 11, 15, and 20 of embryonic advancement (through the hatching period), with 1?time and 4 and 10?weeks posthatch. (clinical-research-informatics.com)
Results1
However, northern blot analysis of this 477bp sequence yielded no results. (wikipedia.org)
RNAs2
Lacking classical Shine-Dalgarno (SD) sequences, messenger RNAs (mRNAs) of Bacteroidetes were recently shown to be enriched for adenine residues at position -3, -6, and -11 to -15 relative to the translational start codon, with adenine overrepresentation at these positions positively correlating with translation efficiency 8 . (nature.com)
In Proteobacteria, small regulatory RNAs (sRNAs) can regulate target mRNAs through imperfect base-pairing interactions that typically occlude the SD sequence and/or the start codon, thus interfering with translation initiation 15 . (nature.com)
Promoter sequences1
The consensus recognition sequence of σ ABfr was deduced by manual inspection of 23 promoter sequences in B . fragilis as 'TAnnTTTG' at the -7 region and a 'TTTG' motif at around the -33 region 6 , 7 . (nature.com)
Regulon1
A significant complication in analysis of the global σ 54 regulon in a bacterium is that the σ 54 RNA polymerase holoenzyme requires interaction with an active bacterial enhancer-binding protein (bEBP) to initiate transcription at a σ 54 -dependent promoter. (biomedcentral.com)
Composition2
Quantitative saccharification and inductively coupled plasma emission spectroscopy (ICP-ES) for elemental analysis revealed composition differences between biomass substrates, which may have influenced growth and transcriptomic profiles. (biomedcentral.com)
The present invention relates to the use of oligonucleotides for the preparation of a pharmaceutical composition for the prevention or treatment of a disease, wherein neurogenesis and/or neuroregeneration has a beneficial effect, in particular a neurodegenerative disease like Morbus Alzheimer, Morbus Parkinson incl. (justia.com)
Small1
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). (lookformedical.com)
Found2
A subsequent tiling array analysis sequencing Hfq-binding sRNA found that the Watson strand did indeed encode an sRNA. (wikipedia.org)
DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)
Essential1
The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. (lookformedical.com)
Present1
Repetitive sequences such as transposases and those present in cohesin domains made closing this genome challenging and the genome sequence was not finished until 2007. (biomedcentral.com)
Elements1
An online browser, called 'Theta-Base' ( www.helmholtz-hiri.de/en/datasets/bacteroides ), is launched to interrogate the obtained gene expression data and annotations of ~4500 transcription start sites, untranslated regions, operon structures, and 269 noncoding RNA elements. (nature.com)
Component1
The DNA microarray gave a greater number (2,351) of significant genes relative to RNA-seq (280 genes when normalized by the kernel density mean of M component (KDMM) method) in an analysis of variance (ANOVA) testing method with a 5% false discovery rate (FDR). (biomedcentral.com)