Cluster BiogenesisMitochondrialClustersSubunitEvolutionarilyMitochondriaPathwaysGenesPlastidRibosomalSynthesisHomeostasisMaturationStructuralOxidationMetabolismRedoxExpressionMembrane-boundKineticARGONAUTEFerrous ironCellSubunitsRegulationGenomeExonsRibosomesComplexesTranscriptionalCatalyzeMetabolicDegradation2022MechanismsPhysiologicalSequenceEssentialGenePlaysCellsSystemHumansYersiniaMethodsProgressionExperimentalHumanFamilyProcess
Cluster Biogenesis2
- Using the Iron Sulfur cluster biogenesis in S. cerevisiae as a test case we indicate how this procedure can be used to analyze and validate the network model against experimental results. (biomedcentral.com)
- Iron-Sulfur Cluster biogenesis) or complex pathways with an unclear reaction and regulation network, (e. g. cell cycle). (biomedcentral.com)
Mitochondrial9
- This gene encodes a mitochondrial protein, which is evolutionarily conserved. (wikipedia.org)
- GLRX5 is a mitochondrial protein is conserved evolutionarily and plays a role in the formation of iron-sulfur clusters, which function to maintain iron homeostasis within the mitochondria and in the cell. (wikipedia.org)
- In human mitochondria, almost all mitochondrial proteins originate from the nucleus, except for 13 subunit proteins that make up the crucial system required to perform 'oxidative phosphorylation (OX PHOS)', which are expressed by the mitochondria's self-contained DNA. (bvsalud.org)
- Recently, increasing evidence suggests that mitochondrial dysfunction plays a critical role in the development and progression of OIDs and its associated systemic diseases. (bvsalud.org)
- As a test case, we reconstruct the topology of the reaction and regulatory network for the mitochondrial ISC biogenesis pathway in S. cerevisiae . (biomedcentral.com)
- A putative role for frataxin in directly regulating mitochondrial iron import is discarded from our analysis, which agrees with also published experimental results. (biomedcentral.com)
- To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with selective inhibition of mitochondrial Complex III and identified OCIA domain-containing protein 1 (OCIAD1) as a Complex III assembly factor. (biorxiv.org)
- We find that OCIAD1 is an inner mitochondrial membrane protein that forms a complex with supramolecular prohibitin assemblies. (biorxiv.org)
- Here, we review mechanisms of mitochondrial gene expression with a focus on the recent findings in the field of mammalian mtDNA transcription and disease phenotypes caused by defects in proteins involved in this process. (biomed.news)
Clusters9
- It is involved in the biogenesis of iron- sulfur clusters, which are required for normal iron homeostasis. (wikipedia.org)
- Crystal structure of the GLRX5 protein reveals that the protein likely exists as a tetramer with two Fe-S clusters buried in the interior. (wikipedia.org)
- Structural organization of essential iron-sulfur clusters in the evolutionarily highly conserved ATP-binding cassette protein ABCE1. (nature.com)
- Along with its most important role as central reductant, T[SH] 2 have also been assumed to regulate the activation of iron-sulfur cluster proteins (Fe/S). Fe/S clusters are versatile cofactors of various proteins and execute a much broader range of essential biological processes viz. (frontiersin.org)
- One such class of pathways is involved in the biogenesis of Iron-Sulfur Clusters (ISC). (biomedcentral.com)
- Iron-sulfur (Fe-S) clusters are essential cofactors present in all known forms of life and required by hundreds of proteins to perform their function. (curefa.org)
- CF27-specific gene clusters which have been analyzed were expressed by both ferrous iron-grown and sulfur-attached cells, indicating that they are not pseudogenes and may play a role in both situations. (frontiersin.org)
- After focusing for some time on the capacity of several plant GRXs to assemble \[2Fe-2S\] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. (hal.science)
- This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. (hal.science)
Subunit2
- Compared to the pre-splitting state, we observe repositioning of ABCE1's iron-sulfur cluster domain, which rotates 150° into a binding pocket on the 40S subunit. (nature.com)
- Construction of the Central Protuberance and L1 stalk during 60S subunit biogenesis Mol. (db-engine.de)
Evolutionarily1
- The function of GLRX5 is highly conserved evolutionarily. (wikipedia.org)
Mitochondria1
- Mitochondria lacking Hem25p failed to efficiently incorporate IPP into early CoQ precursors, leading to loss of CoQ and turnover of CoQ biosynthetic proteins. (bvsalud.org)
Pathways5
- Hence, we suggest that, by designing highly potent and specific inhibitors of TryR enzyme, inhibition of T[SH] 2 reduction and overall inhibition of most of the downstream pathways including Fe/S protein activation reactions, can be accomplished. (frontiersin.org)
- Increasing amounts of data that can be mined for information about how proteins in cells assemble as metabolic pathways, signal transduction pathways, and gene circuits, are generated each day. (biomedcentral.com)
- The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. (studyres.com)
- We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. (hal.science)
- In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. (hal.science)
Genes4
- 96% of genes in KN400 had clear orthologs with conserved synteny in PCA. (biomedcentral.com)
- Most of the remaining genes were in regions of genomic mobility and were strain-specific or conserved in other Geobacteraceae , indicating that the changes occurred post-divergence. (biomedcentral.com)
- In case you're not sure what is meant by the "genetic code," it refers to how the sequence of bases in DNA (there are four such bases) are translated into amino acids, the constituents of proteins and the products of most genes. (whyevolutionistrue.com)
- miRNAs are either expressed from independent transcriptional units or derive from introns of protein-coding genes or exons or introns of long ncRNAs. (biomedcentral.com)
Plastid2
- Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
- Reiter B, Vamvaka E, Marino G, Kleine T, Jahns P, Bolle C, Leister D and Rühle T (2020) The Arabidopsis Protein CGL20 is Required for Plastid 50S Ribosome Biogenesis. (hhu.de)
Ribosomal1
- The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation. (db-engine.de)
Synthesis3
- GLRX5 activity is required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. (wikipedia.org)
- In pursuit of this aim, a systems biology approach was undertaken to get an insight into the overall picture to unravel how T[SH] 2 synthesis and reduction is linked with the regulation of Fe/S cluster proteins and controls the redox homeostasis at a larger scale. (frontiersin.org)
- Cysteine, a thiol-containing amino acid, is crucial for the synthesis of sulfur-containing biomolecules that control multiple essential cellular activities. (biomed.news)
Homeostasis1
Maturation1
- For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with *Populus trichocarpa* and *Arabidopsis thaliana* as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. (hal.science)
Structural2
- In this approach, mathematical models play a central role for the evaluation of the alternative network structures that arise from literature data-mining, phylogenetic profiling, structural methods, and human curation. (biomedcentral.com)
- Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Nat. (db-engine.de)
Oxidation2
- Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
- has been shown to consist of at least seven species, all of which derive energy from the oxidation of elemental sulfur and reduced inorganic sulfur compounds (RISCs) to support their growth. (frontiersin.org)
Metabolism2
- The bifunctional CODH ( cdhAB-2 ) is predicted to play an ubiquitous role in the metabolism of CO, and a novel nitrate reductase-associated respiratory complex was induced specifically in the presence of sulfate. (hindawi.com)
- HMOX2 is constitutively expressed to encode a 36 kDa HO-2 protein, mainly functioning to maintain the basal heme metabolism and may also play a role in inflammatory responses [ 4 ] . (encyclopedia.pub)
Redox2
- I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. (hal.science)
- Redox post-translational modifications of proteins** In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. (hal.science)
Expression1
- However, activation of heme oxygenase-1 (HO-1) can upregulate ferritin expression, which binds to ferrous iron and detoxifies its pro-oxidant effect. (encyclopedia.pub)
Membrane-bound1
- MFSD1 is a lysosomal membrane-bound solute carrier protein with no previously described function in immunity. (x-mol.com)
Kinetic1
- The more than likely absence of known mechanistic and kinetic data for each of the individual proteins in a novel pathway hinders the process of translating network topology into a mathematical model. (biomedcentral.com)
ARGONAUTE2
- The miRNA duplexes are loaded into an Argonaute protein in the miRNA-induced silencing complex (miRISC) and rapidly unwound. (biomedcentral.com)
- Pre-miRNAs are exported to the cytoplasm by Exportin-5 and processed further by Dicer, to ~22 nt double-stranded miRNA duplexes that are loaded into an Argonaute protein in the miRISC and rapidly unwound. (biomedcentral.com)
Ferrous iron4
- HOs are the key-limiting enzymes in heme degradation leading to carbon monoxide (CO), ferrous iron, and biliverdin products. (encyclopedia.pub)
- Heme is degraded by heme oxygenases (HOs), generating biliverdin, carbon monoxide, and ferrous iron (Fe 2+ ). (encyclopedia.pub)
- Ferrous iron (Fe 2+ ) possesses pro-oxidant activity. (encyclopedia.pub)
- Ferrous iron increases ROS generation via the Fenton reaction. (encyclopedia.pub)
Cell2
- events have receptors stimulated with students( GAGs), unregulated chains docking of a inactive actin, all of an been protein respiration cell a considerable subunits. (evakoch.com)
- This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. (regenerativemedicine.net)
Subunits1
- The essential ATP-binding cassette protein ABCE1 splits 80S ribosomes into 60S and 40S subunits after canonical termination or quality-control-based mRNA surveillance processes. (nature.com)
Regulation1
- Although, several Fe/S cluster proteins and their roles have been identified in Leishmania, some of the components of how T[SH] 2 is involved in the regulation of Fe/S proteins remains to be explored. (frontiersin.org)
Genome1
- Datasets available for such tasks include the primary literature, large scale micro array experiments, whole genome two hybrid screenings, full genome sequences, and the patterns of conserved/non-conserved homologues and orthologues in them. (biomedcentral.com)
Exons1
- The GLRX5 gene contains 2 exons and encodes for a protein that is 13 kDa in size. (wikipedia.org)
Ribosomes1
- Stop" refers to stop codons: when the process of protein-making in the ribosomes encounters this codon, the translation is stopped and the string of amino acids ends. (whyevolutionistrue.com)
Complexes2
- Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes. (db-engine.de)
- Aftertranscription, these fragments guide protein complexes that target foreign nucleic acids and promote their degradation. (pjmonline.org)
Transcriptional2
- Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
- This occurs through the downregulation of methylmalonyl coenzyme A epimerase (MCEE), mediated by an extracellular signal-regulated kinase 2-driven transcription factor Sp1/early growth response protein 1 transcriptional switch driven by metastatic signalling at its promoter level. (biomed.news)
Catalyze1
- The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. (hal.science)
Metabolic1
- The achievement, at a global level, of a "healthy aging phenotype" defined as "the condition of being alive, while having highly preserved functioning metabolic, hormonal and neuroendocrine control systems at the organ, tissue and molecular levels" [ 10 ], is the most ambitious objective that modern science should achieve. (iospress.com)
Degradation1
- Heme oxygenases (HOs) act on heme degradation to produce carbon monoxide (CO), free iron, ferritin, and biliverdin. (encyclopedia.pub)
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Mechanisms1
- Hopfner, K.P. Invited review: architectures and mechanisms of ATP binding cassette proteins. (nature.com)
Physiological1
- Hydrogen peroxide (H2 O2 ) has key signaling roles at physiological levels, while causing molecular damage at elevated concentrations. (biomed.news)
Sequence1
- Substantial sequence differences (at least 12 non-synonymous SNP/kb) were found in 3.6% of the orthologs, and this set was enriched in cytochromes and integral membrane proteins. (biomedcentral.com)
Essential2
- It plays essential role in crop production by stimulating the growth and development. (researchsquare.com)
- Transcription of mtDNA is not only essential for the biogenesis of the OXPHOS system, but also generates RNA primers necessary to initiate mtDNA replication. (biomed.news)
Gene1
- Glutaredoxin 5, also known as GLRX5, is a protein which in humans is encoded by the GLRX5 gene located on chromosome 14. (wikipedia.org)
Plays1
- Formerly OXPHOS was thought to be reduced in tumours and that glycolysis was the critical pathway for generation of ATP but it is now clear that OXPHOS, at least in many tumour types, plays a critical role in delivering the bioenergetic and macromolecular anabolic requirements of cancer cells. (biomed.news)
Cells1
- The protein is highly expressed in erythroid cells. (wikipedia.org)
System3
- protein system, it would be very unlikely that all modern species would have the same code. (whyevolutionistrue.com)
- The clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) system provides the majority of bacteria and archaea with adaptive and hereditary immunity against this threat. (pjmonline.org)
- 2013). However, a unique defense system involving clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) was reported recently. (pjmonline.org)
Humans1
- Plague is a zoonotic disease, endemic throughout the world, and highly infectious in humans. (asm.org)
Yersinia1
- Yersinia genomes had a similar global partition of protein functions, as measured by the distribution of Cluster of Orthologous Groups families. (biomedcentral.com)
Methods1
- To achieve such a goal, strategies that combine the different theoretical and computational methods to identify proteins and generate a set of plausible alternative network topologies for the process of interest are needed. (biomedcentral.com)
Progression1
- Emerging data show the multiple roles of HO-1 in tumorigenesis from pathogenesis to the progression to malignancy, metastasis, and even resistance to therapy. (encyclopedia.pub)
Experimental2
- Predictions regarding how proteins act in ISC biogenesis are validated by comparison with published experimental results. (biomedcentral.com)
- For example, the predicted role of Arh1 and Yah1 and some of the interactions we predict for Grx5 both matches experimental evidence. (biomedcentral.com)
Human1
- Although human disease is rare, Y. pestis is dangerous and highly infectious and thus has been identified as having potential for use in bioterrorism or as a biological weapon. (asm.org)
Family1
- Review: Role of the plant-specific calcium-binding C2-DOMAIN ABSCISIC ACID-RELATED (CAR) protein family in environmental signaling. (hhu.de)
Process1
- For example, the involvement of proteins X, Y and Z in a process does not elucidate if X catalyzes a reaction that produces a substrate for another reaction catalyzed by Z or by Y, or if X modulates Y or Z activity. (biomedcentral.com)