Robotic high throughput flow cytometry. Medical search. Frequent questions

Drug screening efforts have traditionally relied primarily on time-consuming or computationally demanding techniques like ELISA-type assays and high-content imaging cytometry. (labmanager.com)
Our lab is equipped with two ultra-high throughput robotic platforms for both biochemical and cell-based assays. (edu.au)
With the advancement of technology, flow cytometers are now able to detect a much greater range of fluorescent dyes, facilitating the analysis of more extensive and complex multicolor immunophenotyping assays. (bioradiations.com)
Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. (bioradiations.com)
It is capable of complex, programmed, multiple liquid dispenses, and is commonly used to dispense cells in matrices, such are Matrigel, for 3D, high-throughput and high-content assays. (gla.ac.uk)
However, the principles discussed herein are broadly applicable to the processes, experiments, and protocols used to generate high-quality data from clinical LC-MS/MS assays. (annlabmed.org)
1514 Eisenhower Place Ann Arbor, MI 48108-3284, USA Arbor Assays builds the highest quality detection and immunoassay products for clinically important biomolecules. (contractresearchmap.com)

These advances enable high-throughput phenotypic screening and structure-activity relationship screening (SAR) in areas like drug discovery, therapeutics development, and biomarker discovery. (labmanager.com)
In late September of 2014 the TDI expanded our automation portfolio with the installation of a multimillion pound flagship open-access, state-of-the-art, high content phenotypic screening platform as part of the UK - National Phenotypic Screening Centre. (ox.ac.uk)
High content screening is automated image based screening of cells to measure phenotypic changes through external treatment with siRNA or small compounds. (ox.ac.uk)
Flow cytometry in HTS uses fluorescent probes which bind to specific cell associated molecules and allows measurements of various phenotypic, biochemical and molecular characteristics of individual cells. (ox.ac.uk)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
To provide a service platform combining the capacity of generating, on a large-scale basis, mutations in the mouse genome with high-throughput and comprehensive phenotypic analysis of the model. (igbmc.fr)
This double certification concerns our fee-for-service and research and development activities in the following areas: - Genetic engineering for the generation of mouse models - Custom management and experienced breeding of mouse lines -Comprehensive, high-throughput and integrative phenotypic analysis of mouse models. (igbmc.fr)
The Screening Facility couples genetic and chemical high throughput screening with multiparametric phenotypic image analysis to support translational cancer research. (gla.ac.uk)

To successfully carry out a large proportion of the applications mentioned above, you will need a high performance flow cytometer such as the ZE5 Cell Analyzer. (bio-rad.com)
Critical for helping scientists accelerate scientific and drug discoveries in both academia and industry, the ZE5 Cell Analyzer is a high performance, automation-ready flow cytometer with the ideal combination of high-throughput screening and high parameter cell analysis capabilities. (bio-rad.com)
Bio-Rad's high-throughput ZE 5 Cell Analyzer solved this with an integrated wash station that travels with the probe, cleaning it thoroughly between each sample. (labmanager.com)
Instrument blocking has formed another major concern, according to Richard, who reports further innovation in sample pumps along with increased pressure and speed in flow cells within the ZE 5 Cell Analyzer to mitigate this problem. (labmanager.com)
The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery. (bioradiations.com)
Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein-specific antibodies in a rapid, high-throughput manner. (bioradiations.com)
Advanced instrumentation includes a newly acquired iCyt Mission Technology Reflection Cell Sorter with multiple lasers and high speed sorting capabilities housed in a BSL-2 containment environment and a Beckman-Coulter Quanta SC MPL analyzer for highly automated robotic handling of routine flow cytometry analyses. (grantome.com)

These configurable platforms can support diverse assay readout technologies, including high-throughput flow cytometry, high-content imaging, whole-plate fluorometric imaging and traditional plate-reader screens. (edu.au)
We utilize a SCARA robot integrated with an InCell 1000 for our high throughput high content screens. (ox.ac.uk)
The TDI currently utilizes a BD twin laser FACSCalibur with an integrated BD High Throughput Sampler (HTS) for FACS based high throughput screens. (ox.ac.uk)
For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? (bioradiations.com)
In this project, we aim to investigate the extent to which dosage compensation occurs in respond to heterozygous mutations in mammalian systems using high throughput CRISPR screens coupled to single cell RNA sequencing. (sirop.org)
Facilitating this enjoyment of our screens for hours on end, as we so often do, is partly down to quantum dots - the semiconductor nanocrystals with unique optical properties that can withstand hours of electrical activity, and provide us with the high definition and brightness we experience on our screens. (chemistryworld.com)
It is most commonly used to replicate plates, in high-throughput CRISPR, siRNA and compound screens. (gla.ac.uk)
The ANU Centre for Therapeutic Discovery (ACTD) includes state-of-the-art robotic and liquid handling systems that will allow researchers to perform high throughput screens to identify novel treatments for cancer and other diseases. (edu.au)

Therapeutics development, antibody screening, and biomarker discovery demand maximal productivity to reduce time-to-results, which can greatly benefit from high-throughput, high parametric flow analysis interfaced with robotic automation. (bio-rad.com)
Flow cytometry has only recently become a viable method for high-throughput screening. (labmanager.com)
The National Drug Discovery Centre features the latest in robotic high-throughput screening technologies with a highly modular system that can be customised according to project needs. (edu.au)
We offer users access to a diverse screening collection containing more than 100,000 high-quality and lead-like compounds. (edu.au)
This initiative is one of the integrated components of the NIH Molecular Libraries Roadmap initiative that offers biomedical researchers access to large-scale automated high throughput screening (HTS) centers in the MLPCN, diverse compound libraries in the Small Molecule Repository (MLSMR) and information on biological activities of small molecules in the PubChem BioAssay public database. (nih.gov)
In addition to the HTS equipment, the TDI has several siRNA and small compound libraries available to investigators for high throughput screening. (ox.ac.uk)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
High-throughput screening (HTS) is a critical component of the entire drug discovery process. (bioradiations.com)
Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications. (bioradiations.com)
Nanobiotechnology, high-throughput technologies for life cell and in vivo screening of small molecules for tissue targeting, tumor targeting. (biu.ac.il)
As a result of these efforts, the capacity of biologists to perform in vitro high-throughput screening of chemicals for drug discovery was dramatically improved. (biu.ac.il)
The need to find more cost-effective methods of drug development, combined with the recent advances in robotic screening which enable the testing of hundreds of thousands of products per year, has led pharmaceutical companies to examine combinatorial synthetic strategies as a means of accelerating drug discovery programs, and increasing the chemical diversity of their compound libraries. (biu.ac.il)
Chemists in academia are aware that novel synthetic tools must be developed in order to meet the increasing requirements of the high-throughput biological screening technology. (biu.ac.il)
The Access Laboratory Workstation, combined with a one-step qPCR preparation workflow for RT-qPCR can be adapted for high-throughput gene expression screening. (beckman.hk)
With its ability to transfer nanoliter (nL) volumes of samples and reagents accurately and precisely, the Echo Liquid Handler dramatically reduces screening costs - making one-step RT-qPCR viable as a high-throughput screening method. (beckman.hk)
Our Perkin Elmer Opera High-Content Screening system is supported by a robotic plate-loading arm, and JANUS and Wellmate liquid handling systems. (gla.ac.uk)
The Opera Phenix is a high-throughput confocal microscope primarily used for high-content screening and phenotype analysis. (gla.ac.uk)
Previously demonstrated high-throughput screening Raman spectroscopy (HTS-RS) eliminates the complexity as well as human interaction by incorporation full automation of the data acquisition process. (tib.eu)
It is comprised of two separate workstations: a high throughput screening workstation and a high content/imaging workstation. (edu.au)

Here we review the key recent innovations that ensure reliable, high-throughput, automated workflows. (labmanager.com)
BD FACSDiva™ Software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today's busy laboratory. (bdbiosciences.com)

Experience with extracellular vesicle isolation, Flow cytometry, PCRs, Simple Westerns, and CRISPR-CAS9 molecular editing will come with the project. (sirop.org)

Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)

While it has always been ideal for multiparametric analysis of cells based on their physical characteristics and surface protein expression, flow cytometry has traditionally been too slow in processing large sample numbers to gain widespread use in high-volume applications. (labmanager.com)
Like flow cytometry, high-content imaging cytometry is multiparametric, but it requires considerable processing power for analyzing and storing large image data files, and capturing rare events is difficult. (labmanager.com)

If your assay has been demonstrated in 96-well format and meets minimum robustness, signal-to-background and component-stability requirements, our expert staff will miniaturise and adapt it to run on our high-throughput platform. (edu.au)
As your throughput increases, the Biomek automated cell-based assay solutions enable you to obtain results you can rely on, while optimizing your assay workflow and reducing your time at the bench. (beckman.de)

Analysis of a high-quality chromosome-scale genome sequence for the little skate shows that it preserves many ancestral jawed vertebrate features compared with other sequenced genomes, including numerous ancient microchromosomes. (bvsalud.org)
Applying scDyad&T-seq to mouse embryonic stem cells transitioning from serum to 2i conditions, we observe dramatic and heterogenous demethylation and the emergence of transcriptionally distinct subpopulations that are closely linked to the cell-to-cell variability in loss of DNMT1-mediated maintenance methylation activity, with regions of the genome that escape 5mC reprogramming retaining high levels of maintenance methylation fidelity. (deylab.com)

Here, we determined the levels of antibodies, antigen-specific B cells, against a recombinant GFP-tagged SARS-CoV-2 spike (S) protein and total T and NK cell subsets in subjects up to 20 days after the injection of the BNT162b2 (Pfizer-BioNTech) vaccine using a combined approach of serological and flow cytometry analyses. (cdc.gov)

The ability to quantify these has led to flow cytometry being used in a wide range of applications, including but not limited to: Monitoring of CD4 count in HIV Diagnosis of various cancers Analysis of aquatic microbiomes Sperm sorting Measuring telomere length Until the early 2000s, flow cytometry could only measure a few fluorescent markers at a time. (wikipedia.org)

At the same time, microfluidic qPCR methods are providing a flow cytometry-like method of quantifying 48 or more RNA molecules per cell. (wikipedia.org)
Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)

Technological advances over the last two decades have turned flow cytometry into a powerful high-throughput tool for a broad range of large-scale applications requiring rapid multi-parameter analysis. (labmanager.com)

BD FACSDiva™ Software provides features to help users integrate flow systems into application areas, such as index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories. (bdbiosciences.com)
Automation of alignment in photonics device test, production and assembly is crucial for boosting throughput. (globalspec.com)
Development of new technologies for automation and miniaturization of organic synthesis: Multicomponent and multistep organic synthesis on solid supports for high throughput synthesis. (biu.ac.il)
As throughput and the desire to have more rich data early on in the research studies, automation continues to be integral in aiding laboratories manage the seeding, feeding and passaging of cells, that otherwise could be significant time and be error prone. (beckman.de)

The Echo is equipped with a fully articulated robotic arm, integrated centrifuge and Combi bulk-fill reagent dispenser. (ox.ac.uk)

Data standards include the widely adopted Flow Cytometry Standard (FCS) defining how data from cytometers should be stored, but also several new standards under development by the International Society for Advancement of Cytometry (ISAC) to aid in storing more detailed information about experimental design and analytical steps. (wikipedia.org)
Flow cytometers operate by hydrodynamically focusing suspended cells so that they separate from each other within a fluid stream. (wikipedia.org)
Traditional flow cytometers need to fill the sample line up to the interrogation point with material from a single sample, which is time consuming. (labmanager.com)
The index sorting feature (available on BD Sorters Flow Cytometers) enables laboratories to sort cells into single wells of plates and identify them on dot plots, in statistics views and in a detailed results file. (bdbiosciences.com)

Learn the principles of exosome detection using flow cytometry, and access a range of useful resources on small particle analysis, including a handy protocol. (bio-rad.com)
He brings special expertise to the detection and high-speed sorting of rare cell subpopulations including a number of newly evolving and complementary cell separation technologies. (grantome.com)

He is also an expert in advanced data analysis techniques for analysis of complex multicolor flow cytometric data. (grantome.com)
As an alternative to automated microscopy, urinary flow cytometric data analysis has helped in the differentiation of distinct urinary microorganisms. (medgadget.com)

The rapid increase in the dimensionality of flow cytometry data, coupled with the development of high-throughput robotic platforms capable of assaying hundreds to thousands of samples automatically have created a need for improved computational analysis methods. (wikipedia.org)

Truly high-throughput capabilities are now possible off-the-shelf through advanced complimentary instrument and software solutions. (labmanager.com)
However, developing truly high-throughput capabilities requires the systematic removal of process bottlenecks from sample loading through data analysis. (labmanager.com)

Specific duties include tissue cell culture, flow cytometry, PCR, western blots, immunofluorescence, animal work, preparation of data for publication and grant proposals, ordering of supplies and equipment, upkeep of equipment and general lab maintenance. (hospitalcareers.com)

Flow cytometry can be used to quantify the number and proportion of specific cell types in a heterogeneous mixture, by examining the expression of proteins often expressed on the surface of cells. (bio-rad.com)

Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing flow cytometry data using extensive computational resources and tools. (wikipedia.org)
The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. (wikipedia.org)
Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (wikipedia.org)
Finally, diagnosis using flow cytometry data can be aided by supervised learning techniques, and discovery of new cell types of biological importance by high-throughput statistical methods, as part of pipelines incorporating all of the aforementioned methods. (wikipedia.org)
Open standards, data and software are also key parts of flow cytometry bioinformatics. (wikipedia.org)
Flow cytometry data is in the form of a large matrix of intensities over M wavelengths by N events. (wikipedia.org)
Other established methods, such as flow cytometry, can then provide extra data regarding immune cell phenotype, activation, and function. (technologynetworks.com)
For experiments which may require a more intense X-ray beam, such as for high throughput data collection, or a tunable X-ray source, the facility also has rapid access to the SSRL synchrotron at Stanford University. (cedars-sinai.edu)
However, current Raman-based characterization of pollen is complex and time-consuming, resulting in low throughput and limiting the statistical significance of the acquired data. (tib.eu)

Flow cytometry is a key technology in the field of cancer research, but presents challenges for flow cytometry instrumentation, especially when studying solid tumors or dealing with a high volume of samples. (bio-rad.com)

A recent study reported that the number can be even higher (up to 3000 genes) that display signs of haploinsuffeciency (Collins et al, Cell, 2023). (sirop.org)

Therefore, investigating how the PCa PMN unfolds will give us a deeper understanding of the biology of metastasis and lead to the discovery of novel drug targets essential for tailoring treatments for patients at higher risk of PCa metastasis and reducing tumor-related mortality. (sirop.org)

Instruments based on flow cytometry and microscopy generate effective and clinically useful results, whereas the reading of automated test strip provides further value. (medgadget.com)

This Flow Cytometry and Cell Separation Shared Resource is viewed as a synergistic facility that will interface with a developing, new campus-wide institutional Imaging Facility and the existing proteomics facility. (grantome.com)

Master thesis or technician position: High-throughput investigation of dosage compensation to heterozygous mutations in mammalian systems. (sirop.org)
Changing the type of markers used, from STRs to single nucleotide polymorphisms (SNPs), may result in increased success from more forensic samples and be more adaptable to high-throughput and/or miniaturized typing systems. (biomedcentral.com)
From simple on-deck devices to complete robotic systems, we can develop focused, yet flexible, Biomek-based solutions by integrating Beckman Coulter and third-party instruments. (beckman.de)

The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. (justia.com)

High Throughput - Includes dual-arm wafer-swapping robotics. (coherent.com)

For instance, you've got a protein that would normally be expressed on the surface of the cell-if you take it off the surface of the cell and put it onto a plate, then potentially you're changing the conformation of that protein," explains Richard Cuthbert, PhD, flow cytometry product manager at Bio-Rad. (labmanager.com)
Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
WaferLase II performs high-speed, high-precision silicon wafer etching, marking, and glass scribing for NGS Flow Cell production with automated tools. (coherent.com)
We discover bifunctional domains that can both activate and repress, some of which dynamically split a cell population into high- and low-expression subpopulations. (bvsalud.org)
The Flow Cytometry and Cell Separation Shared Resource is newly evolved from the former Analytical Cytology Shared Resource and began operations in March of 2009. (grantome.com)
The new shared resource takes advantage of the recent recruitment of Dr. James Leary to the Cancer Center who is a nationally recognized leader in the field of flow cytometry and cell separation. (grantome.com)
Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
Moreover, even when high-purity single-cell sorting can be achieved, viability after sorting is often low to moderate, especially for cell types that are particularly sensitive to hydrodynamic stress or low-density culture conditions (e.g., primary cells or pluripotent stem cell lines). (nature.com)

Optional Robotic Loading - Automated side loading/unloading of parts. (coherent.com)

The demand for high-throughput flow cytometry solutions offering rapid multiparameter analysis is not surprising, given the context. (labmanager.com)
A number of important performance parameters such as throughput, association probability, empirical cumulative distribution function (CDF), signal to interference noise ratio (SINR), percentile of user rate, and varying bandwidth are considered in the analysis. (amrita.edu)
Analysis of urine particle which is microscopy-based has progressed steadily over the years, enabling clinical laboratories' throughput. (medgadget.com)
Unfortunately, such devices do not allow for high throughput analysis due to the manual effort involved in operating the valving mechanisms. (justia.com)

The DNA sensor strains can identify diverse bacterial species including major human pathogens with high specificity. (bvsalud.org)

Faster changeover between liquids leads to higher throughput and maximum device utilization. (labx.com)

SPECIFIC INFECTIONS Necrotizing soft tissue infections Diagnosis and management of primary, secondary, and tertiary peritonitis High-risk patients: Who are they and how should they be managed? (docksci.com)

Both of these workstations are equipped with robotic arms and liquid handling instruments that will allow researchers to perform a variety of different tasks (e.g. seeding cells into microplates and performing drug treatments) on a large scale. (edu.au)

Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. (wikipedia.org)
Flow Cytometry is an extremely effective method of recognizing and assessing characteristics of cells or particles. (bio-rad.com)
Flow cytometry may be used to separate and sort cells based on various detectable characteristics, which has many uses in research. (bio-rad.com)
Connors and his team first used flow cytometry to isolate B cells from an HIV-infected donor. (the-scientist.com)

More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
Over her academic career, she has been Member of the National Medicinal Chemistry Committee, Director of the PhD course in Pharmaceutical Sciences at the Doctorate School of Science and High Technology at the University of Torino, Director of the undergraduate course in Herbal Medicine Technology, Member of the Committee of the European School of Medicinal Chemistry (ESMEC) and, since 2012, Director of the School of Hospital Pharmacy. (stratagem-cost.eu)
Although there are reports of COVID-19 vaccine implementation in real-world populations, these come from high-income countries or from experience with messenger RNA technology vaccines. (cdc.gov)

New attention towards removing the last of the bottlenecks associated with high-throughput flow cytometry has produced accessible, comprehensive commercial solutions. (labmanager.com)

The amount of this intrinsic absorption can be used to determine the physiological health of an organ-for example, whether the tissue is getting sufficient oxygenated blood flow. (nationalacademies.org)

Flexible in Shape Variable arrangements of actuators or actuator arrays will enable different types of deformations with highest resolution. (globalspec.com)
Flexible in Feature Size Utilizing piezo ceramic material with a structured composition will enable highest operational flexibility and shaping of the smallest features. (globalspec.com)

ISLANDIA, NY - Festo introduces the DHOP closed-loop pipette head for high-speed robotic pipetting applications requiring consistent volumes over a wide range of liquid viscosities. (labx.com)

Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. (wikipedia.org)
Rapid growth in emerging economies and technological development in the urinalysis diagnostics sphere with the advent of portable and battery-operated urinalysis devices are key factors contributing to high CAGR of the market during the forecast period. (medgadget.com)

In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and image-based confluence measurements for automated dilution ratio calculation. (frontiersin.org)
This poses a serious barrier for the adaptation in real-world scenarios as they require large datasets of high-quality real images within the desired area. (epfl.ch)
PHENOMIN has been approved by Lloyd's register Quality Assurance to the following Quality Management System Standards NF EN ISO 9001:2015. (igbmc.fr)

High Speed Rock Drilling (HSRD), a Swiss company, has been developing and testing an efficient process for deep drilling down to 10 km. (sirop.org)

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