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  • binds
  • We found that CMTr1 binds ATP-dependent RNA DHX15 helicase and that this interaction, mediated by the G-patch domain of CMTr1, has an advantageous effect on CMTr1 activity towards highly structured RNA substrates. (royalsocietypublishing.org)
  • Binds specifically to the terminal m3G-cap U snRNAs. (uniprot.org)
  • Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. (uniprot.org)
  • In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. (uniprot.org)
  • Nuclear export of RNA is regulated by the cap binding complex (CBC), which binds exclusively to 7-methylguanylate-capped RNA. (wikipedia.org)
  • Following Dicer cleavage, an Argonaute (Ago) protein binds to the short RNA duplexes, forming the core of a multi-subunit complex called the RNA-induced silencing complex (RISC). (wikipedia.org)
  • mRNAs
  • Shatkin "Capping of eucaryotic mRNAs" 1976 Cell 9:645-653. (freepatentsonline.com)
  • In eukaryotes, the 5′ ends of most messenger RNAs (mRNAs) are blocked, or "capped. (justia.com)
  • Capped mRNAs are translated in cell-free translation systems more efficiently than are non-capped mRNAs. (justia.com)
  • These results highlight the potential for discovery of mechanism-based antimalarial drugs designed to specifically block the capping of Plasmodium mRNAs. (pnas.org)
  • In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. (uniprot.org)
  • RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. (uniprot.org)
  • This is in contrast to the amastin mRNA in amastigotes and to reporter RNAs lacking the URE, which, in common with most eukaryotic mRNAs studied to-date, are deadenylated before being degraded. (nih.gov)
  • Once miR-155-5p/-3p is assembled into the RISC, these molecules subsequently recognize their target messenger RNA (mRNA) by base pairing interactions between nucleotides 2 and 8 of miR-155-5p/-3p (the seed region) and complementary nucleotides predominantly in the 3'-untranslated region (3'-UTR) of mRNAs (see Figure 4 and 5 below). (wikipedia.org)
  • bacterial
  • While these processes are well understood in the messenger RNA of cells in higher organisms, Prof. Dr Andres Jäschke and his bioorganic chemistry working group now revealed these mechanisms in bacterial RNA. (xonl.de)
  • replication
  • The sequence similarities of RNA replication genes and strategies for BMV have been shown to extend to a wide range of plant and animal viruses beyond the alphaviruses, including many other positive-strand RNA viruses from other families. (wikipedia.org)
  • Flavivirus genomic RNA replication occurs on rough endoplasmic reticulum membranes in membranous compartments. (wikipedia.org)
  • Diaz, A., Gallei, A., and Ahlquist, P. Bromovirus RNA Replication Compartment Formation Requires Concerted Action of 1a's Self-Interacting RNA Capping and Helicase Domains. (wikipedia.org)
  • subunit
  • ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. (uniprot.org)
  • initiation
  • This unique molecular structure called cap0 protects capped RNAs from 5′ to 3′ exonuclease cleavage and is essential for the regulation of gene expression, including splicing, nuclear export of mRNA, and translation initiation [ 3 - 5 ]. (royalsocietypublishing.org)
  • transcript
  • Figure 1 shows a time course experiment that plots yield of a 3.7 kb capped transcript over time. (thermofisher.com)
  • The MIR155HG RNA transcript does not contain a long open reading frame (ORF), however, it does include an imperfectly base-paired stem loop that is conserved across species. (wikipedia.org)
  • modification
  • Once these questions are answered and biologists have a better sense of the amount of variation in RNA modification, the focus will turn to each modification's biological function. (wikipedia.org)
  • NADH
  • The mechanism of capping with NAD+, NADH, or 3'-dephospho-coenzyme A is different. (wikipedia.org)
  • Capping with NAD+, NADH, or 3'-dephospho-coenzyme A is targeted by promoter sequence. (wikipedia.org)
  • Capping with NAD+, NADH, or 3'-dephospho-coenzyme A occurs only at promoters that have certain sequences at and immediately upstream of the transcription start site and therefore occurs only for RNAs synthesized from certain promoters. (wikipedia.org)
  • molecular
  • Cofactor Genomics , a software-driven biotechnology company developing unique molecular diagnostics centered on RNA, has launched a clinically-validated oncology assay which provides unique insight into a patient's cancer profile. (rna-seqblog.com)
  • In 2016, after multi-year efforts to standardize and certify Cofactor's foundational molecular protocols for clinical use, Cofactor became one of the first laboratories to receive CAP-accreditation for RNA-seq. (rna-seqblog.com)
  • Jon Armstrong, Cofactor's CSO will present "Reaching the Pinnacle: A Unique Cancer Diagnostic Tool that Harnesses the Power of RNA" on Tuesday, February 21 at 11:45 am - 12:15 pm in the Molecular Diagnostics track. (rna-seqblog.com)
  • Epitranscriptomics describes an aspect of molecular genetics, or a study thereof, that depends on biochemical modifications of RNA. (wikipedia.org)
  • nuclear
  • Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. (uniprot.org)
  • 211-221 AND 323-327 , FUNCTION IN U SNRNP NUCLEAR IMPORT, INTERACTION WITH KPNB1, RNA-BINDING, SUBCELLULAR LOCATION. (uniprot.org)
  • reference needed) Small nuclear RNAs contain unique 5'-caps. (wikipedia.org)
  • helicase
  • It uses the helicase ATP hydrolysis site to remove the γ-phosphate from the 5′ end of the RNA. (wikipedia.org)
  • phosphorylation
  • After successful capping, an additional phosphorylation event initiates the recruitment of machinery necessary for RNA splicing, a process by which introns are removed to produce a mature mRNA. (wikipedia.org)
  • degradation
  • Very little is known as to how these cis-elements regulate RNA turnover and translation rates in trypanosomatids and nothing is known about mRNA degradation mechanisms in Leishmania in particular. (nih.gov)
  • RNase-H-RNA blot analysis revealed that a major part of the rapid promastigote-specific degradation of the amastin mRNA is not initiated by deadenylation. (nih.gov)
  • Capping with 7-methylguanylate prevents 5′ degradation in two ways. (wikipedia.org)
  • positive-stranded
  • Brome mosaic virus (BMV) is a small (28 nm, 86S), positive-stranded, icosahedral RNA plant virus belonging to the genus Bromovirus, family Bromoviridae, in the alphavirus-like superfamily. (wikipedia.org)
  • nucleoside
  • The cap contains a 5′-5′ triphosphate linkage between two nucleoside moieties and a 7-methyl group on a distal guanine ring. (justia.com)
  • Complex
  • Recognizing RNA's ability to identify relevant targets in cancer drug development, as well as more accurately predict the correct treatment for cancer patients, are the key reasons we have built a company committed to developing technologies to interpret the complex data represented by RNA. (rna-seqblog.com)
  • The goal of the proposed research is to understand in detail the role of the cap binding complex (CBC) in mRNA processing and transport. (grantome.com)
  • Genetic and biochemical approaches will be used to identify factors that interact with CBC and to determine how these factors influence the dynamics and function of the mRNA cap binding complex in S. cerevisiae. (grantome.com)
  • During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. (uniprot.org)
  • mechanisms
  • Conventional and unconventional mechanisms for capping viral mRNA. (univ-mrs.fr)
  • In contrast, the atomic structures and catalytic mechanisms of the fungal ( 6 ) and mammalian (A. Changela, C.K.H., A. Martins, S.S., and A. Mondragon, unpublished observations) RNA triphosphatases are completely different. (pnas.org)
  • modifications
  • There are more than one hundred documented RNA modifications, which are described in a database maintained by the RNA Institute at the University at Albany. (wikipedia.org)
  • There are three known types of RNA modifications. (wikipedia.org)
  • viral
  • The viral genome is around 2500 nm in length so we can therefore conclude that it must be tightly packaged within the capsid along with substances such as sodium ions in order to cancel out the negative charges on the RNA caused by the phosphate groups. (wikipedia.org)
  • although substantially less virulent than if contained within the viral particle, the RNA can have increased infectivity when transfected into cells. (wikipedia.org)
  • mRNA
  • In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. (wikipedia.org)
  • screw cap
  • The 2ml screw cap microcentrifuge tubes are available as conical bottom without a skirt for easy viewing of contents at the bottom after centrifugation and with a skirt as a self -standing tube. (genelink.com)
  • Each package is securely scaled by screw cap and is identifiable by a batch number and packaging date. (sigmaaldrich.com)