• mRNAs
  • Shatkin "Capping of eucaryotic mRNAs" 1976 Cell 9:645-653. (freepatentsonline.com)
  • In eukaryotes, the 5′ ends of most messenger RNAs (mRNAs) are blocked, or "capped. (justia.com)
  • Capped mRNAs are translated in cell-free translation systems more efficiently than are non-capped mRNAs. (justia.com)
  • In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. (uniprot.org)
  • Once miR-155-5p/-3p is assembled into the RISC, these molecules subsequently recognize their target messenger RNA (mRNA) by base pairing interactions between nucleotides 2 and 8 of miR-155-5p/-3p (the seed region) and complementary nucleotides predominantly in the 3'-untranslated region (3'-UTR) of mRNAs (see Figure 4 and 5 below). (wikipedia.org)
  • bacterial
  • While these processes are well understood in the messenger RNA of cells in higher organisms, Prof. Dr Andres Jäschke and his bioorganic chemistry working group now revealed these mechanisms in bacterial RNA. (xonl.de)
  • enzymes
  • The enzymes of this superfamily share the following similarities: Conserved regions known as motifs I, II, III, IIIa, IV, V and VI, which are arranged in the same order and similar spacing A lysine containing motif KxDG (motif I) A covalent lysyl-NMP intermediate The capping enzyme is composed of two domains, a nucleotidyl transferase (NTase) domain and a C-terminal oligonucleotide binding (OB) domain. (wikipedia.org)
  • The NTase domain, conserved in capping enzymes, DNA and RNA ligases, is made up 5 motifs, I, III, IIIa, IV and V. Motif I or KxDG is the active site where the covalent (lysyl)-N-GMP intermediate is formed. (wikipedia.org)
  • vitro
  • The ability to synthesize capped RNA molecules in vitro is useful, because it allows workers to prepare RNA molecules that behave properly in a variety of biological applications. (justia.com)
  • Capped RNAs thus produced are active in splicing reactions carried out in vitro. (justia.com)
  • Recognition of cap structure in splicing in vitro of mRNA precursors. (justia.com)
  • snRNAs
  • In addition, there are some other forms of RNA that are also capped, for instance small nuclear RNAs (snRNAs). (justia.com)
  • Sm-class snRNAs are found with 5'-trimethylguanosine caps, while Lsm-class snRNAs are found with 5'-monomethylphosphate caps. (wikipedia.org)
  • NADH
  • In bacteria, and potentially also in higher organisms, some RNAs are capped with NAD+, NADH, or 3'-dephospho-coenzyme A. In all organisms, mRNA molecules can be decapped in a process known as messenger RNA decapping. (wikipedia.org)
  • The mechanism of capping with NAD+, NADH, or 3'-dephospho-coenzyme A is different. (wikipedia.org)
  • Capping with NAD+, NADH, or 3'-dephospho-coenzyme A is targeted by promoter sequence. (wikipedia.org)
  • Capping with NAD+, NADH, or 3'-dephospho-coenzyme A occurs only at promoters that have certain sequences at and immediately upstream of the transcription start site and therefore occurs only for RNAs synthesized from certain promoters. (wikipedia.org)
  • phosphorylation
  • After successful capping, an additional phosphorylation event initiates the recruitment of machinery necessary for RNA splicing, a process by which introns are removed to produce a mature mRNA. (wikipedia.org)
  • transcript
  • The MIR155HG RNA transcript does not contain a long open reading frame (ORF), however, it does include an imperfectly base-paired stem loop that is conserved across species. (wikipedia.org)
  • helicase
  • It uses the helicase ATP hydrolysis site to remove the γ-phosphate from the 5′ end of the RNA. (wikipedia.org)
  • modification
  • Once these questions are answered and biologists have a better sense of the amount of variation in RNA modification, the focus will turn to each modification's biological function. (wikipedia.org)
  • sequence
  • Sequence analysis of small RNA clone libraries comparing miRNA expression to all other organ systems examined established that miR-155-5p was one of five miRNAs (i.e. miR-142, miR-144, miR-150, miR-155, and miR-223) that was specific for hematopoietic cells including B-cells, T-cells, monocytes and granulocytes. (wikipedia.org)
  • Complex
  • Recognizing RNA's ability to identify relevant targets in cancer drug development, as well as more accurately predict the correct treatment for cancer patients, are the key reasons we have built a company committed to developing technologies to interpret the complex data represented by RNA. (rna-seqblog.com)
  • nucleoside
  • The cap contains a 5′-5′ triphosphate linkage between two nucleoside moieties and a 7-methyl group on a distal guanine ring. (justia.com)