• binds
  • Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. (uniprot.org)
  • In the CBC complex, ncbp2/cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ncbp1/cbp80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. (uniprot.org)
  • The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (By similarity). (uniprot.org)
  • Nuclear export of RNA is regulated by the cap binding complex (CBC), which binds exclusively to 7-methylguanylate-capped RNA. (wikipedia.org)
  • Following Dicer cleavage, an Argonaute (Ago) protein binds to the short RNA duplexes, forming the core of a multi-subunit complex called the RNA-induced silencing complex (RISC). (wikipedia.org)
  • proteins
  • Shatkin "mRNA cap binding proteins: essential factors for initiating translation" 1985 Cell 40: 223-4. (freepatentsonline.com)
  • The ARCA modification ensures that the S atom is precisely positioned within the active sites of cap-binding proteins in both the translational and decapping machinery. (justia.com)
  • Following addition of 1 mg of recombinant PB2 cap-binding domain proteins, incubation was performed for 30 min at 30 °C. Anacardic acid and garcinol showed strong inhibition of the binding activity of recombinant PB2 with [14C]acetyl-CoA. (nih.gov)
  • Coronavirus replication is known to affect cellular translation, involving activation of stress-induced signaling pathways, and employing viral proteins that affect cellular mRNA translation and RNA stability. (pubmedcentralcanada.ca)
  • These mini-factories are called ribosomes, and are complicated machines built from RNA and proteins of various sorts. (heavenforum.org)
  • Pre-mRNA undergoes a series of modifications - 5' capping, splicing and 3' polyadenylation before becoming mature mRNA that exits the nucleus to be translated into functional proteins and capping of the 5' end is the first of these modifications. (wikipedia.org)
  • The addition of the cap onto mRNA confers protection to the transcript from exonucleases that degrade unprotected RNA and assist in the nuclear export transport process so that the mRNA can be translated to form proteins. (wikipedia.org)
  • Diaz, A., and Ahlquist, P. Role of Host Reticulon Proteins in Rearranging Membranes for Positive-Strand RNA Virus Replication. (wikipedia.org)
  • This has already been investigated in a select few proteins such as adenosine deaminase, which acts on RNA (ADAR). (wikipedia.org)
  • The cap in mRNA is very important it helps the to recognition by cap-binding proteins to initiate translation, and prevents the degradation of mRNA in the cytosol. (wikipedia.org)
  • single-stranded
  • Flaviviruses share several common aspects: common size (40-65 nm), symmetry (enveloped, icosahedral nucleocapsid), nucleic acid (positive-sense, single-stranded RNA around 10,000-11,000 bases), and appearance in the electron microscope. (wikipedia.org)
  • mRNAs
  • Shatkin "Capping of eucaryotic mRNAs" 1976 Cell 9:645-653. (freepatentsonline.com)
  • In eukaryotes, the 5′ ends of most messenger RNAs (mRNAs) are blocked, or "capped. (justia.com)
  • Capped mRNAs are translated in cell-free translation systems more efficiently than are non-capped mRNAs. (justia.com)
  • In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. (uniprot.org)
  • Once miR-155-5p/-3p is assembled into the RISC, these molecules subsequently recognize their target messenger RNA (mRNA) by base pairing interactions between nucleotides 2 and 8 of miR-155-5p/-3p (the seed region) and complementary nucleotides predominantly in the 3'-untranslated region (3'-UTR) of mRNAs (see Figure 4 and 5 below). (wikipedia.org)
  • bacterial
  • While these processes are well understood in the messenger RNA of cells in higher organisms, Prof. Dr Andres Jäschke and his bioorganic chemistry working group now revealed these mechanisms in bacterial RNA. (xonl.de)
  • enzymes
  • The enzymes of this superfamily share the following similarities: Conserved regions known as motifs I, II, III, IIIa, IV, V and VI, which are arranged in the same order and similar spacing A lysine containing motif KxDG (motif I) A covalent lysyl-NMP intermediate The capping enzyme is composed of two domains, a nucleotidyl transferase (NTase) domain and a C-terminal oligonucleotide binding (OB) domain. (wikipedia.org)
  • The NTase domain, conserved in capping enzymes, DNA and RNA ligases, is made up 5 motifs, I, III, IIIa, IV and V. Motif I or KxDG is the active site where the covalent (lysyl)-N-GMP intermediate is formed. (wikipedia.org)
  • Methylation
  • It has been recently discovered that some modification like RNA methylation, may play an active role in regulating some biological process. (wikipedia.org)
  • The four sites on mRNA for methylation are N7-methylguanine (at the 5′ cap), N6-methyl adenosine, 5-methylcytosine, and 2′-O-methylation. (wikipedia.org)
  • NADH
  • The mechanism of capping with NAD+, NADH, or 3'-dephospho-coenzyme A is different. (wikipedia.org)
  • Capping with NAD+, NADH, or 3'-dephospho-coenzyme A occurs only at promoters that have certain sequences at and immediately upstream of the transcription start site and therefore occurs only for RNAs synthesized from certain promoters. (wikipedia.org)
  • phosphorylation
  • After successful capping, an additional phosphorylation event initiates the recruitment of machinery necessary for RNA splicing, a process by which introns are removed to produce a mature mRNA. (wikipedia.org)
  • transcript
  • The MIR155HG RNA transcript does not contain a long open reading frame (ORF), however, it does include an imperfectly base-paired stem loop that is conserved across species. (wikipedia.org)
  • helicase
  • It uses the helicase ATP hydrolysis site to remove the γ-phosphate from the 5′ end of the RNA. (wikipedia.org)
  • modification
  • Once these questions are answered and biologists have a better sense of the amount of variation in RNA modification, the focus will turn to each modification's biological function. (wikipedia.org)
  • nucleoside
  • The cap contains a 5′-5′ triphosphate linkage between two nucleoside moieties and a 7-methyl group on a distal guanine ring. (justia.com)