SequencesGenesRRNAEscherichiaNucleotidesRibosomesRole in ribosomalProteinsPolymeraseProtein synthesisBacteriaMitochondrialAmplificationRibonucleicOligonucleotideMammalian cellsMethyltransferaseSynthesisPhylogenyBiologySubunitsBacterialMoleculesMethylationProbeRibosomeStructuralCharacterizationPurityHelicaseTranscriptionBoundFluorescentAbundantGenomeParasiteDetectionSmall
Sequences6
- All ribosomal proteins (including the specific sequences that bind to rRNA) have been identified. (wikipedia.org)
- A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
- A. Ribosomal RNA analysis StructRNAfinder - predicts and annotates RNA families in transcript or genome sequences. (printerresource.com)
- In the class that I'm teaching, we're going to be looking at ribosome structures to see if the polymorphisms that we find in the sequences of 16S ribosomal RNA are related antibiotic resistance. (molecule-world.com)
- Berg K.L. , Squires C. , Squires C.L. Ribosomal RNA operon anti-termination Function of leader and spacer region box B-box A sequences and their conservation in diverse microorganisms. (microbiologyresearch.org)
- PCR based on the amplification of the small sub-unit ribosomal RNA (ssrRNA) gene sequences was applied. (who.int)
Genes8
- Relative abundance profiles of bacterial populations measured by sequencing DNA or RNA of marker genes can widely differ. (doe.gov)
- Distel, D. L., DeLong, E. F., and Waterbury, J. Epub … Bacterial Identification by 16S rRNA Gene Sequence Analysis Background All bacteria contain 16S ribosomal RNA (rRNA) genes of approximately 1500 base pairs (bp) in length. (printerresource.com)
- rRNA genes contain regions of variable DNA sequence that are unique to the species carrying the … Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosome subunits. (printerresource.com)
- Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
- The bacterial genes encoding chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) were transiently expressed in leaf discs from the AR1 promoter in TGMV A. The levels of AR1 and GUS RNAs were similar in leaf discs after adjusting for viral DNA copy number, while CAT RNA was less abundant. (ncsu.edu)
- The geminivirus transient expression system allows rapid analysis of RNAs transcribed from foreign genes and can serve as a preliminary screen in the construction of transgenic plants. (ncsu.edu)
- Chromatin structure and expression of plant ribosomal RNA genes. (ncsu.edu)
- Baylis H.A. , Bibb M.J. Organization of the ribosomal RNA genes in Streptomjces coelicolor A3(2). (microbiologyresearch.org)
RRNA16
- Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA which is the primary component of ribosomes, essential to all cells. (wikipedia.org)
- rRNA is the physical and mechanical factor of the ribosome that forces transfer RNA (tRNA) and messenger RNA (mRNA) to process and translate the latter into proteins. (wikipedia.org)
- Ribosomes are composed of approximately 60% rRNA and 40% ribosomal proteins by mass. (wikipedia.org)
- Because of these configurations, rRNA can form tight and specific interactions with ribosomal proteins to form ribosomal subunits. (wikipedia.org)
- Ribosomal proteins can also cross-link to the sugar-phosphate backbone of rRNA with binding sites that consist of basic residues (i.e. lysine and arginine). (wikipedia.org)
- Eukaryotic rRNA has over 70 ribosomal proteins which interact to form larger and more polymorphic ribosomal units in comparison to prokaryotes. (wikipedia.org)
- LSU rRNA subtypes have been called ribozymes because ribosomal proteins cannot bind to the catalytic site of the ribosome in this area (specifically the peptidyl transferase center, or PTC). (wikipedia.org)
- High purity total ribosomal RNA (rRNA). (plantmedia.com)
- The most widely understood role of RNA is in protein synthesis, which includes messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA) (see Chapter 2). (printerresource.com)
- To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA transcripts isoforms and processing sites. (printerresource.com)
- Base modifications occur primarily in tRNA and ribosomal RNA (rRNA), and occur after the RNA is transcribed. (printerresource.com)
- The most highly enriched messenger RNAs encoded proteins required for the Pol I RNA pre-initiation complex required for ribosomal RNA (rRNA) transcription, (PolR1A and upstream binding transcription factor). (ox.ac.uk)
- In vitro, miR-424-5p reduced the expression of these RNAs, reduced rRNA levels, and inhibited protein synthesis. (ox.ac.uk)
- Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins. (colorado.edu)
- Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
- The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)
Escherichia3
- TY - JOUR T1 - Structural characterization of Escherichia coli ribosomal protein S8 and its binding site in 16S ribosomal RNA. (unboundmedicine.com)
- Boros I. , Csordés-Tôth E. , Kiss A. , Tôrôk I. , Udvardy K. , Venetianer P. Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli. (microbiologyresearch.org)
- Brosius J. , Dull D.J. , Sleeter D.D. , Noller H.F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. (microbiologyresearch.org)
Nucleotides1
- The numerous modified nucleotides in eukaryotic ribosomal RNA. (printerresource.com)
Ribosomes1
- Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. (jefferson.edu)
Role in ribosomal1
- Dyskerin is composed of 514 amino acids and has a role in ribosomal RNA processing and telomere maintenance. (medscape.com)
Proteins12
- Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosome subunits. (wikipedia.org)
- it makes up about 80% of cellular RNA despite never being translated into proteins itself. (wikipedia.org)
- These ribosomal proteins contain basic residues (as opposed to acidic residues) and aromatic residues (i.e. phenylalanine, tyrosine and tryptophan) allowing them to form chemical interactions with their associated RNA regions, such as stacking interactions. (wikipedia.org)
- These interactions along with the association of the small and large ribosomal subunits result in a functioning ribosome capable of synthesizing proteins. (wikipedia.org)
- These are combined with ~50 ribosomal proteins to form ribosomal subunits. (wikipedia.org)
- RESULTS: Pull-down assays showed that miR-424-5p bound to messenger RNAs encoding proteins associated with muscle protein synthesis. (ox.ac.uk)
- RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
- Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
- RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
- We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
- After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
- This RNA attaches (binds) to several proteins, forming an enzyme complex called mitochondrial RNA-processing endoribonuclease, or RNase MRP. (medlineplus.gov)
Polymerase6
- 4. That is, RNA polymerase II reaches forward to find the start point, and RNA polymerase III reaches backward. (printerresource.com)
- RNA polymerase III promoters differ significantly from RNA polymerase II promoters in that they are located downstream from the transcription start site and within the transcribed segment of the DNA. (printerresource.com)
- METHODS: Targets of miR-424-5p were identified by Argonaute2 pull down, and the effects of the miRNA on RNA and protein expression were determined by quantitative polymerase chain reaction and western blotting in muscle cells in vitro. (ox.ac.uk)
- This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. (ncsu.edu)
- Nucleic acid amplification (NAA) tests, such as polymerase chain reaction (PCR) and other methods for amplifying DNA and RNA, may facilitate rapid detection of microorganisms. (cdc.gov)
- Process fluid s were examined using culture, polymerase chain reaction, and 16S ribosomal RNA sequencing. (cdc.gov)
Protein synthesis3
- The involvement of RNA in such fundamental processes as protein synthesis and RNA processing has led to the idea that ribozymes were more common in early life. (printerresource.com)
- miR-424-5p reduces ribosomal RNA and protein synthesis in muscle wasting. (ox.ac.uk)
- Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
Bacteria4
- 6. I want to study ribosomal RNA expression and potential processing sites in bacteria. (printerresource.com)
- A., 1992, The use of 16S ribosomal RNA targeted oligonucleotide probes to study competition between ruminai fibrolytic bacteria. (printerresource.com)
- Bacteria 16S ribosomal RNA gene, partial sequence. (nih.gov)
- The diversity of their fecal bacteria was explored using 16S ribosomal RNA gene sequencing. (frontiersin.org)
Mitochondrial3
- Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
- We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
- Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. (ncl.ac.uk)
Amplification1
Ribonucleic1
- and assignment of the genera Cardiobacterium, Dichelobacter, and Suttonella to Cardio-bacteriaceae fam nov in the gamma division of Proteobacteria based on 16S ribosomal ribonucleic acid sequence comparisons. (microbiologyresearch.org)
Oligonucleotide1
- Oligonucleotide models of ribosomal RNA domains are powerful tools to study the binding and molecular recognition of antibiotics that interfere with bacterial translation. (escholarship.org)
Mammalian cells1
- Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
Methyltransferase1
- We show that CplR contributes to intrinsic pleuromutilin, lincosamide and streptogramin A resistance in Clostridioides, and demonstrate that C. difficile CplR (CDIF630_02847) synergises with the transposon-encoded 23S ribosomal RNA methyltransferase Erm to grant high levels of antibiotic resistance to the C. difficile 630 clinical isolate. (lu.se)
Synthesis2
- Synthesis of AR1 RNA was dependent on T-DNA transfer and TGMV DNA replication, demonstrating that it is a plant transcription product. (ncsu.edu)
- Synthesis and assembly of ribosomal components are fundamental cellular processes and generally well-conserved within the main groups of organisms. (nord.no)
Phylogeny1
Biology1
Subunits1
- Mature rRNAs are packaged into 40S and 60S ribosomal subunits for export to the cytoplasm. (nature.com)
Bacterial2
- These differences, made apparent when calculating ribosomal RNA:DNA ratios, have been interpreted as variable activities of bacterial populations. (doe.gov)
- The chemical structure of bacterial ribosomal RNA. (wikidata.org)
Molecules1
Methylation1
Ribosome3
- Techniques such as selective chemical modification, fluorescence labeling and mutations are cumbersome for the whole ribosome but readily applicable to model RNAs, which are readily crystallized and often give rise to higher resolution crystal structures suitable for detailed analysis of ligand-RNA interactions. (escholarship.org)
- We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop modulation. (nature.com)
- Traditionally, the nucleolar Pol I and nucleoplasmic Pol III are viewed as the sole mammalian RNA polymerases that directly mediate housekeeping ribosome biogenesis. (nature.com)
Structural1
- High-resolution crystal structure analysis confirmed that the HX RNA is a faithful structural model of the ribosomal target. (escholarship.org)
Characterization1
- This application note presents the characterization of ribosomal RNA using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering. (chromatographyonline.com)
Purity1
- RNA purity is essential for all the different transcriptomic procedures. (printerresource.com)
Helicase1
- A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit. (colorado.edu)
Transcription1
Bound1
- To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
Fluorescent2
- A bio-orthogonal chemistry-based approach for fluorescent labelling of ribosomal RNA is described. (rsc.org)
- Solution studies showed that HX RNA carrying a fluorescent 2-aminopurine modification provides a model system that can be used to monitor ligand binding to both the ribosomal decoding site and, through an indirect effect, the hygromycin B interaction region. (escholarship.org)
Abundant1
- The most abundant form of RNA. (jefferson.edu)
Genome2
- Two other major roles for noncoding RNA are in genome defense and in genetic regulation. (printerresource.com)
- Using an RNA genome, many viruses are known to encode their genetic information. (printerresource.com)
Parasite1
- In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts. (jove.com)
Detection1
Small1
- Ribosomal RNA organizes into two types of major ribosomal subunit: the large subunit (LSU) and the small subunit (SSU). (wikipedia.org)