• mRNA
  • MICROB Enrich employs hybridization capture technology (patent pending) to remove human, mouse, and rat RNA (both mRNA and rRNA) from complex host-bacterial RNA populations, leaving behind enriched microbial total RNA. (bio-medicine.org)
  • If desired, upon completion of the MICROB Enrich procedure bacterial mRNA can be purified from the total RNA through the seamless integration of Ambion's MICROB Express technology. (bio-medicine.org)
  • The absence of poly(A) tails on bacterial mRNA prevents the use of oligo(dT) primed reverse transcription, which is at the heart of most amplification methods. (thermofisher.com)
  • As little as 10 ng of enriched bacterial mRNA or 100 ng of total RNA can be amplified to yield sufficient antisense amplified RNA (aRNA) for microarray analysis. (thermofisher.com)
  • Co‐immunoprecipitation of cellular RNA followed by deep sequencing (RIP‐seq) has yielded semi‐quantitative snapshots of sRNA and mRNA association patterns with Hfq in different growth phases of Salmonella (Chao et al , 2012 ). (embopress.org)
  • GRIL‐seq works similarly but additionally seeks to seal the typically short sRNA-mRNA duplexes in vivo by expressing an RNA ligase prior to RNA capture (Han et al , 2016 ). (embopress.org)
  • DNA TATA box Pribnow box SOS box CAAT box CCAAT box Operator (biology) Upstream activation sequence RNA List of cis-regulatory RNA elements Polyadenylation signals, mRNA AU-rich element, mRNA Other Regulation of gene expression cis-regulatory module Gene regulatory network Operon Promoter Trans-acting factor Rfam Transterm Butler JE, Kadonaga JT (October 2002). (wikipedia.org)
  • The ibs gene is on the opposite strand to sib and is completely complimentary, so the antisense-binding of Sib RNA with the ibs mRNA brings about dsRNA-mediated degradation. (wikipedia.org)
  • rRNA
  • look OK, and I can clearly see the 23S and 16S rRNA bands in my RNA prep. (bio.net)
  • molecules
  • they are 50- to 500-nucleotide non-coding RNA molecules, highly structured and containing several stem-loops. (wikipedia.org)
  • These molecular helpers remove the protective cap at the start of ribonucleic acid (RNA) molecules. (xonl.de)
  • rifampicin
  • Mutations in rpoB that confer resistance to rifamycins do so by altering residues of the rifamycin binding site on RNA polymerase, thereby reducing rifamycin binding affinity for rifamycins Initial studies were done by Jin and Gross to generate rpoB mutations in E. coli that conferred resistance to rifampicin. (wikipedia.org)
  • Rifampicin, also known as rifampin, is an antibiotic used to treat several types of bacterial infections, including tuberculosis, leprosy, and Legionnaire's disease. (wikipedia.org)
  • genomic
  • For microarray analysis and other sensitive applications, Ambion also recommends a DNase treatment (such as with DNA free Treatment and Removal Reagents ) to remove contaminating genomic DNA from the total RNA prior to the MICROB Enrich procedure. (bio-medicine.org)
  • Typical DNA Yields with the GenElute Bacterial Genomic DNA Kit. (sigmaaldrich.com)
  • Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. (iss-store.co.uk)
  • These new genomic loci were referred to as smr, for S. meliloti RNA. (wikipedia.org)
  • small
  • The lack of an appropriate method for bacterial RNA amplification has hampered studies that rely on rare or limited samples, such as environmental collections, small volume cultures, and bacterial RNA isolated from host cells. (thermofisher.com)
  • There has recently been a surge in studies, including one by Waters et al ( 2017 ) in this issue of The EMBO Journal , that have used clever variations of the RNA ‐seq technique to comprehensively map small RNA -target networks. (embopress.org)
  • The small RNA nitrogen stress-induced RNA 1 (NsiR1) is produced by Cyanobacteria under conditions of nitrogen deprivation. (wikipedia.org)
  • This method involves fusing RNA aptamers that bind metabolites to Spinach 2 , a 98-nt RNA that switches on the fluorescence of 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI), an otherwise nonfluorescent small molecule. (pubmedcentralcanada.ca)
  • 1984 Rajneeshee bioterror attack AsrC small RNA Bacterial small RNA HilD 3'UTR PinT small RNA Typhoid Mary Giannella RA (1996). (wikipedia.org)
  • It was originally identified along with 26 other non-coding RNAs using a tiled Caulobacter microarray protocol specifically aimed at detecting small RNAs. (wikipedia.org)
  • agarose
  • RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose gel electrophoresis. (bio-medicine.org)
  • A 1 µg aliquot of DNA from each respective bacterial sample was resolved on a 1% agarose gel in 0.5X TBE at 150 volts for 16 hours using a BioRad CHEF DRII system. (sigmaaldrich.com)
  • Northern blotting can reveal possible sRNA transcript size and expression levels by running a mixed RNA sample on an agarose gel and probing for a desired sRNA. (wikipedia.org)
  • infections
  • Such conditions, commonly referred to as acquired immunodeficiency syndrome (AIDS), or AIDS related complex (ARC), make HIV patients vulnerable to opportunistic infections such as bacterial, fungal, viral, protozoal, and neoplastic diseases, which ultimately lead to death. (mdpi.com)
  • Although Tuberculosis therapy remains the most important use of Rifampin, an increasing problem with serious Multiple Drug Resistant bacterial infections has led to some use of antibiotic combinations containing Rifampin to treat them. (wikipedia.org)
  • Phages were discovered to be antibacterial agents and were used in the former Soviet Republic of Georgia (pioneered there by Giorgi Eliava with help from the co-discoverer of bacteriophages, Felix d'Herelle) and the United States during the 1920s and 1930s for treating bacterial infections. (wikipedia.org)
  • cellular
  • Cellular RNA is extracted and converted to cDNA, which is used to prepare sequencing libraries. (illumina.com)
  • experiments
  • Even with a short 6 hour IVT incubation time, 100 ng of input total RNA results in sufficient aRNA for several replicate array experiments. (thermofisher.com)
  • protocol
  • Subsequently, the resulting hybrid RNAs of either protocol are sequenced, allowing in silico analysis of sRNA-target interactions. (embopress.org)
  • 2016
  • Holmqvist et al , 2016 ), showing more precisely where Hfq recognizes its RNA targets. (embopress.org)