• mRNA
  • Protein synthesis is an ancient, conserved, complex multienzyme system, involving the participation of hundreds of macromolecules in which the mRNA template is decoded into a protein sequence on the ribosome. (hindawi.com)
  • The fate of the mRNA after termination and ribosome recycling is highly relevant for translation of multicistronic mRNAs. (asmscience.org)
  • The in vitro data suggest that when termination of protein synthesis is followed by the action of RRF, EF-G, and IF3 the 30S particle remains attached to the mRNA, allowing the latter to diffuse long distances along the subunit. (asmscience.org)
  • rRNA
  • In 1993, Koonin and Rudd predicted the spoU gene product to be an RNA methyltransferase based on the similarity of its deduced amino acid sequence to that of Streptomyces azureus Tsr [ 1 ], which catalyzes methylation of the ribose of adenosine (A) at position 1067 in 23S rRNA to form 2'- O -methyladenosine (Am1067) [ 4 ]. (pubmedcentralcanada.ca)
  • In yeast, the 18S, 5.8S, and 25S ribosomal RNAs (rRNAs) are transcribed as a single 35S precursor rRNA. (genetics.org)
  • rRNAs
  • The RNA ligase catalyzed addition of radioactively labeled cytidine 3',5'-bisphosphate has been used to probe the availability of the 3' ends of rRNAs in the ribosome under varying conditions. (illinois.edu)
  • inhibitory
  • In the absence of RF3, the inhibitory action of RF1 on ribosome recycling can be reduced by increasing the ribosome-recycling factor (RRF) concentration, suggesting that whatever the action of RRF may be, it is incompatible with the presence of RF1 on the ribosome. (asmscience.org)
  • yeast
  • In yeast, a K51R mutation in eIF5A, in which lysine at position 51 is replaced by arginine ( 14 ), not only inhibits hypusine formation but also cannot rescue eIF5A knockouts ( 15 ) and has reduced affinity for the ribosome ( 16 ). (pubmedcentralcanada.ca)
  • antibiotic
  • The antibiotic does not affect the ribosome binding of EF-G·GTP or GTP hydrolysis, whereas it strongly inhibits translocation and blocks EF-G turnover after GTP hydrolysis and translocation. (asmscience.org)
  • Structure
  • Compared to the crystal structure of isolated T. thermophilus RF2 ( 15 ), the factor in complex with the ribosome has undergone major conformational changes. (pubmedcentralcanada.ca)
  • Fitting the more detailed model of YidC into this overall structure of the whole complex revealed how a single YidC protein might interact with the ribosome to insert a newly built protein into a membrane. (elifesciences.org)
  • factors
  • We suggest that the proximal consequence of these LTV1 mutations is inhibition of the cytoplasmic maturation of 40S subunits and that nuclear retention of pre-40S subunits is a downstream consequence of the failure to release and recycle critical factors back to the nucleus. (genetics.org)
  • More than 150 trans -acting factors have been identified by genetic and proteomic studies with roles in ribosome biogenesis. (genetics.org)
  • Both precursor subunits exit the nucleus accompanied by a small contingent of biogenesis factors that are presumed to function either in export or in subsequent cytoplasmic maturation steps. (genetics.org)
  • From the comparison of the binding of all these factors to the ribosome, it appears that they use the same anchoring points on the ribosome and make contact with the same GTPase-associated center while differing substantially in their functions. (asmscience.org)
  • Activity
  • RIBOSOME biogenesis is a major biosynthetic activity of eukaryotic cells and a significant rate-limiting factor in cell growth and proliferation. (genetics.org)
  • several
  • This ∼64-subunit, ATP-dependent proteolytic machine employs several Ub receptors that recognize the Ub polymers and then degrades the modified target concomitant with the DUB -directed release of the Ub moieties for reuse ( Finley, 2009 ). (plantcell.org)
  • similar
  • Electron-microscopic images of individual ribosomes are aligned by multireference alignment techniques (a), and similar images are grouped into classes by multivariate statistical classification procedures. (asmscience.org)