Loading...
  • solvents
  • In the chemical and pharmaceutical sciences, both phases usually are solvents. (wikipedia.org)
  • The partition coefficient, abbreviated P, is defined as a particular ratio of the concentrations of a solute between the two solvents (a biphase of liquid phases), specifically for un-ionized solutes, and the logarithm of the ratio is thus log P. When one of the solvents is water and the other is a non-polar solvent, then the log P value is a measure of lipophilicity or hydrophobicity. (wikipedia.org)
  • mobile phase
  • The eluate is the mobile phase leaving the column. (wikipedia.org)
  • The mobile phase is the phase that moves in a definite direction. (wikipedia.org)
  • The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. (wikipedia.org)
  • The solvent is allowed to completely evaporate off to prevent it from interfering with sample's interactions with the mobile phase in the next step. (wikipedia.org)
  • The addition of micelles to the mobile phase introduces a third phase into which the solutes may partition. (wikipedia.org)
  • A high CMC would require a high concentration of surfactant which would increase the viscosity of the mobile phase, an undesirable condition. (wikipedia.org)
  • Additionally, a Kraft point should be well below room temperature to avoid having to apply heat to the mobile phase. (wikipedia.org)
  • In the capillary inlet interface, the evaporation of the mobile phase inside the capillary was one of the main issues. (wikipedia.org)
  • interaction
  • A corresponding partition coefficient for ionizable compounds, abbreviated log P I, is derived for cases where there are dominant ionized forms of the molecule, such that one must consider partition of all forms, ionized and un-ionized, between the two phases (as well as the interaction of the two equilibria, partition and ionization). (wikipedia.org)
  • refers
  • RPC refers to liquid (rather than gas) chromatography. (wikipedia.org)
  • The partition-coefficient generally refers to the concentration ratio of un-ionized species of compound whereas the distribution-coefficient refers to the concentration ratio of all species of the compound (ionized plus un-ionized). (wikipedia.org)
  • capillary
  • It may be a liquid (LC and Capillary Electrochromatography (CEC)), a gas (GC), or a supercritical fluid (supercritical-fluid chromatography, SFC). (wikipedia.org)
  • To run a thin layer chromatography plate, the following procedure is carried out: Using a capillary, a small spot of solution containing the sample is applied to a plate, about 1.5 centimeters from the bottom edge. (wikipedia.org)
  • column
  • In addition to the liquid chromatography and mass spectrometry devices, an LC-MS system contains an interface that efficiently transfers the separated components from the LC column into the MS ion source. (wikipedia.org)
  • partition
  • Partition coefficients can also be defined when one of the phases is solid, for instance, when one phase is a molten metal and the second is a solid metal, or when both phases are solids. (wikipedia.org)
  • process
  • Plates can be labeled before or after the chromatography process using a pencil or other implement that will not interfere or react with the process. (wikipedia.org)