• Separation of these species is not possible except via molecular techniques such as polymerase chain reaction (PCR). (medscape.com)
  • An addition to the existing requirements was made by introducing a new molecular technique, called mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC), for the quality control of the vaccine. (who.int)
  • ABSTRACT This study aimed to establish an accurate and sensitive polymerase chain reaction (PCR) technique for the diagnosis of active human brucellosis in Egypt. (who.int)
  • In context of surgery, conceptual understanding of alterations in interactions between host defenses and pathogenic microbes that result in bacterial urinary tract infections (UTI) are important for at least two reasons. (ivis.org)
  • Organisms seen by Gram stain yielded a sensitivity of 96.6%, specificity of 88.9%, positive predictive value of 97.7% and negative predictive value of 84.2% when used to predict positive culture results for bacterial wound infection. (who.int)
  • The Gram stain method is one of the most commonly used techniques to quickly diagnose bacterial infections. (medlineplus.gov)
  • This can be done with a simple swab (bacterial culture), a needle or syringe, or with a biopsy. (medlineplus.gov)
  • Bacterial culture has high sensitivity and high specificity, is relatively low cost, is suitable for different types of specimen sources, and can be used for epidemiologic and resistance testing purposes. (medscape.com)
  • This paper aims to summarize existing methods of cultured meat production, especially 3D bioprinting of cultured meat, which is an emerging approach with unique advantages. (researchgate.net)
  • Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
  • The advancements and limitations of cultured meat based on tissue engineering, 3D printing of meat, and 3D bioprinting of cultured meat are discussed. (researchgate.net)
  • It is clear that the renewed use of these windmills, spinning round across the landscape, would not only improve local agriculture and aesthetic, but also provide an ecologically sound technique for mill restoration and re-use elsewhere in Europe. (coe.int)
  • Results (1) Cultured meat is an ecologically sustainable alternative to conventional meat. (researchgate.net)
  • [1] An infectious disease , also known as a transmissible disease or communicable disease , is an illness resulting from an infection. (wikipedia.org)
  • ABSTRACT To determine the microbiology of wound infection following caesarean section and to evaluate the use of Gram stain for the predicton of subsequent microbiological culture results, 1319 surgical wounds were followed up. (who.int)
  • However, performing the more sensitive PCR assay-based tests to raise the likelihood of detecting an infection and then following up with culture to produce admissible evidence is appropriate. (medscape.com)
  • Preimplantation genetic testing (PGT) is a technique used to identify chromosomal genetic abnormalities in embryos created through in vitro fertilization (IVF) before pregnancy. (medscape.com)
  • Since these organisms require highly specialized conditions for culture in vitro and because molecular-based detection is not widely available (except for M genitalium), clinicians must be aware of the clinical syndromes with which these organisms have been associated and have a high level of suspicion prompting them to order the appropriate diagnostic tests from reference laboratories. (medscape.com)
  • Cultured meat (i.e., meat produced in vitro using tissue engineering techniques) is being developed as a potentially healthier and more efficient alternative to conventional meat. (researchgate.net)
  • The broadening scope of evolution from simple genes to more abstract concepts, such as designs and behaviors makes the idea of evolutionary culture more plausible. (wikipedia.org)
  • However, cybersecurity culture has historically been seen as an abstract concept and difficult to quantify. (cxotoday.com)
  • The modern approach to IVF involves blastocyst culture and biopsy followed by PGT and a single embryo transfer. (medscape.com)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • In comparison to conventionally produced European meat, cultured meat involves approximately 7-45% lower energy use (only poultry has lower energy use), 78-96% lower GHG emissions, 99% lower land use, and 82-96% lower water use depending on the product compared. (researchgate.net)
  • We did Gram stains and cultures on exudates from open wounds and on aspirates if the wounds had demonstrable fluid collection. (who.int)
  • RESUME Afin de déterminer la microbiologie de l'infection de la plaie après une césarienne et d'évaluer l'utilisation de la coloration de Gram pour prévoir les résultats des cultures microbiologiques ultérieures, 1319 plaies chirurgicales ont fait l'objet d'un suivi. (who.int)
  • Nous avons procédé à une coloration de Gram et à des cultures sur des exsudats de plaies ouvertes et des échantillons prélevés par aspiration si la plaie avait une accumulation de fluides manifeste. (who.int)
  • Les micro-organismes mis en évidence par coloration de Gram ont donné une sensibilité de 96,6 %, une spécificité de 88,9 %, une valeur prédictive positive de 97,7 % et une valeur prédictive négative de 84,2 % lorsqu'ils étaient utilisés pour prévoir les résultats de culture positifs pour les infections bactériennes des plaies. (who.int)
  • Gram stains, cultures, and nucleic acid amplification tests (NAATs) of genital, rectal, conjunctival, pharyngeal secretions and/or other extragenital sites, as applicable, should also be obtained when DGI is suspected. (medscape.com)
  • 2 Several clinical isolates of RSV were evaluated for ribavirin susceptibility by plaque reduction in tissue culture. (nih.gov)
  • Many results drawn from previous studies of the effect of antibiotic use on emergence, selection and spread of AMR have lacked a holistic view combining all aspects into one study. (europa.eu)
  • Antibiotic susceptibility testing to selected antibiotics was done using Kirby-Bauer disk diffusion technique. (who.int)
  • Bacillus cereus is a pathogenic bacterium that causes food contamination, resulting in food poisoning such as diarrhea and emesis. (cabi.org)
  • logic recovery of pathogenic organisms by Purulent exudates were obtained from culture. (who.int)
  • The lack of these receptors in laboratory hosts during vaccine production significantly decreases yields, resulting in vaccine mismatch that increases antigenic drift. (europa.eu)
  • The lack of these receptors in our laboratory hosts to create vaccines significantly dampen yields, the resulting mismatched vaccines do not afford proper protection and further drive antigenic drift. (europa.eu)
  • Cultured meat is created in a laboratory, and the utilization of natural antimicrobials can reduce the demand for antibiotics (Gyawali & Ibrahim, 2014). (researchgate.net)
  • Pipetting is one of the most basic yet critical techniques in the laboratory. (bionity.com)
  • New assays and biotechnologies, including physicochemical techniques, are also evaluated by the Expert Committee, as appropriate, through collaborative laboratory studies. (who.int)
  • Manual of basic techniques for a health laboratory. (who.int)
  • Manual of laboratory techniques. (who.int)
  • Blood culture is the gold standard for with bioterrorism there are laboratory safety microbial diagnosis. (who.int)
  • According to the LCA of cultured meat, it has the potential to reduce land use by 99%, water use by 82-96%, energy use by 7-45%, and GHG emissions by 78-96% compared with conventional European meat (Tuomisto & de Mattos, 2011) . (researchgate.net)
  • The other study indicated that replacing conventional meat with cultured meat may reduce GHG emissions associated with current meat production by 85% in China (Sun et al. (researchgate.net)
  • Objective Cultured meat is considered to be a viable alternative to conventional flesh to satisfy the increasing human demand for meat. (researchgate.net)
  • To overcome these limitations, we developed a technique. (cabi.org)
  • The technique showed high sensitivity, spe- cificity and accuracy. (who.int)
  • Whether in DNA analysis, cell culture or biochemical assays, correct pipetting significantly influences the quality of the results. (bionity.com)
  • In addition to basic statistical evaluation, the results will be catalogued and documented to illustrate the findings and justify further study. (nova.edu)
  • The anticipated results may guide clinical and policy decisions to ultimately reduce the burden of AMR in Europe. (europa.eu)
  • Results: It was notable that waterpipe smokers were significantly associated with a history of oral infections. (who.int)
  • Culture is the criterion standard test in the workup of gonorrheal infections. (medscape.com)
  • One benefit of NAAT assay is that it does not require live organisms yet can yield a positive result with minimal genetic material available, using specimens such as first catch urine and vaginal swabs. (medscape.com)
  • Clinicians have learned that current microbial analysis (culture) often does not provide a clear picture of the clinically significant offending organisms. (nova.edu)
  • To help overcome this challenge, Infosec, a cybersecurity education company helping IT and security professionals, comes up with a survey to classify cybersecurity culture and systematically measure results, allowing organizations to turn this important security variable into a data-driven element in their cybersecurity strategy. (cxotoday.com)
  • For example, GNRs allow for the use of a variety of characterization techniques including transmission and scanning electron microscopy, Raman spectroscopy, inductively coupled plasma mass spectrometry (ICP-MS), and spectroscopy. (springer.com)
  • Perform a culture or nonculture detection test for N gonorrhoeae on endocervical, urethral, pharyngeal, or rectal discharge. (medscape.com)
  • This illustrated history of big-game hunting in the 19th century West pairs a fine narrative using writings of original participants with insights on early sport hunting techniques used among different peoples in the West. (midwestbookreview.com)
  • The PCR positivity increased significantly with increasing seropositivity titres by the standard tube agglutination test and showed 100% positivity in patients with positive blood cultures. (who.int)
  • Culturing was done within 1 hour showed predominantly white blood cells using standard bacteriological inoculation and no organisms [ 6,7 ]. (who.int)
  • 4) There have been preliminary attempts to use 3D bioprinting technology for cultured meat production. (researchgate.net)
  • Another drawback is that 24-72 hours is needed before a preliminary culture result may return. (medscape.com)
  • Only the first positive blood culture of a given spe- resistant Enterococcus (VRE) in many patients in the commu- cies was included for a single patient throughout the study nity (13-16). (cdc.gov)
  • An important area of study for animal culture is vocal learning, the ability to make new sounds through imitation. (wikipedia.org)
  • Upon his return to Hong Kong, Wong sought apprenticeship and immersed himself in the study of Lingnan ceramic culture. (china.org.cn)
  • The study further states that, all organizations and cultures are unique. (cxotoday.com)
  • Also corporate cultures are often driven or reinforced by senior leadership, the study shows. (cxotoday.com)
  • By discussing the advantages and shortcomings of the existing techniques, the prospect for the future development of cultured meat is provided. (researchgate.net)
  • Just like human society that developed a wide array of cultures and technology based on their history and environmental influences, the different orchid genera also have evolved growth mechanisms that allowed them to thrive in their native habitats. (rv-orchidworks.com)
  • This buttermilk culture makes a thick, old-fashioned New England Style, Buttermilk. (cheesemaking.com)
  • Through his exploration, he discovered that the traditional method of glazing ceramics resulted in thick and reflective petals. (china.org.cn)
  • Surgery, dangerous oral medications, topical antifungals, laser therapies and benign neglect offer poor results. (nova.edu)
  • California Department of Health Services, San Francisco, Cali- blood cultures. (cdc.gov)
  • blood culture. (who.int)
  • Blood cultures yield positive culture results in 10-30% of patients and joint fluid in 20-30% of patients. (medscape.com)
  • While the exact number of refugees in Australia experiencing complex trauma is not directly stated in the search results, it is well-documented that refugees and asylum seekers are typically exposed to multiple potentially traumatic events in the context of war, persecution, and displacement. (ceh.org.au)
  • A global team of regionally based and native-speaking trainers use their understanding of the culture, language and context. (echonet.org)
  • His pieces respect the history and culture of glasswork, while also infusing contemporary context and perspectives. (rossanaorlandi.com)
  • One of these techniques resulting in an acceptable computational time and accuracy is the discretisation of the particle size. (hindawi.com)
  • Organizations that utilize the four aforementioned aspects of culture are the ones that are the most successful. (wikipedia.org)
  • Studies have shown that organizations with strong cybersecurity cultures experience increased visibility into potential threats, reduced cyber incidents and greater post-attack resilience among other benefits. (cxotoday.com)
  • By culturing the cream you can add a rich flavor and create cultured butter. (cheesemaking.com)
  • Ultimately, it aims to help you create a culture of trauma-informed care at your workplace and improve engagement and outcomes for clients, especially those who have experienced trauma due to their refugee status. (ceh.org.au)
  • Help you foster a positive and proactive workplace culture that is sensitive to people who have experienced trauma, including refugees. (ceh.org.au)
  • Through my artistry, I endeavor to bridge cultures and foster a deeper appreciation for the beauty that lies in diversity," Wong said. (china.org.cn)
  • Results demonstrate that inhaled MWCNT, which deposit in the lungs, are transported to the parietal pleura, the respiratory musculature and the systemic organs in a singlet form and accumulate with time following exposure. (cdc.gov)
  • In cell cultures the inhibitory activity of ribavirin for respiratory syncytial virus (RSV) is selective. (nih.gov)
  • Aspiration pneumonia results from aspiration of colonized upper respiratory tract secretions. (medscape.com)
  • Methods The potential ecological sustainability of cultured meat is evaluated in order to determine the necessity for its development. (researchgate.net)
  • Medicolegal cases (eg, child abuse, rape) require culture due to the possibility of false-positive results with nonculture methods. (medscape.com)
  • Culture is increasingly seen as a process, involving the social transmittance of behavior among peers and between generations. (wikipedia.org)
  • A further definition of culture is, "[s]ocially transmitted behavior patterns that serve to relate human communities to their ecological settings. (wikipedia.org)
  • 3. Behavior changes, such as altered work technique and increased muscle tension. (cdc.gov)
  • Nous recommandons d'utiliser la PCR comme solution autre que la culture pour le diagnostic de la brucellose. (who.int)
  • Life cycle assessment (LCA) research method was used for assessing environmental impacts of large-scale cultured meat production. (researchgate.net)
  • The survey leverages the latest research into security culture assessment to help our clients show the impact of training beyond measurements like phishing click rates and training completion. (cxotoday.com)
  • Since culture is a form of adaptation to one's environment, it is mirrored in many aspects of our current and past societies. (wikipedia.org)
  • The results are visually rich narratives that demonstrate how each young artist connects with their roots. (phxart.org)
  • Interestingly, when encapsulated cells were co-cultured with THP-1 macrophages, co-cultures exhibited TNF-α production enhancement. (springer.com)
  • The results showed that production of 1000 kg cultured meat requires 26-33 GJ energy, 367-521 m(3) water, 190-230 m(2) land, and emits 1900-2240 kg CO(2)-eq GHG emissions. (researchgate.net)
  • Despite high uncertainty, it is concluded that the overall environmental impacts of cultured meat production are substantially lower than those of conventionally produced meat. (researchgate.net)
  • Farnsworth mashed-up the concepts of a field and a display screen, and the resulting new concept was the key breakthrough in the development of television. (innovationmanagement.se)
  • Future trends in 3D bioprinting of cultured meat are predicted, as well as potential challenges in this field. (researchgate.net)
  • [ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
  • Evidence for animal culture is often based on studies of feeding behaviors, vocalizations, predator avoidance, mate selection, and migratory routes. (wikipedia.org)