• NS5A
  • The HCV protein expression was verified by Western blot and immunofluorescence assays using antibodies specific for HCV NS3 and NS5A proteins. (jove.com)
  • Analysis of molecular surfaces suggests the location of the membrane interaction surface of NS5A, as well as hypothetical protein and RNA binding sites. (centrallakesclinic.biz)
  • genome
  • These trVLPs can continuously infect cells expressing the Ebola virus proteins responsible for genome replication and transcription, allowing us to safely model multiple infectious cycles under biosafety level 2 conditions. (jove.com)
  • interferon
  • The responses included strong HCV antigen-specific interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-2 (IL-2) induction, robust CD4 and CD8 T cell proliferation, and induction of polyfunctional T cells. (wikipedia.org)
  • NSs induces a shut-off of host transcription including interferon (IFN)-beta mRNA 7,8 and promotes degradation of double-stranded RNA-dependent protein kinase (PKR) at the post-translational level. (jove.com)
  • In this study, we show that CD81 cross-linking via immobilized E2 or mAbs specific for CD81 inhibits not only non major histocompatibility complex-restricted cytotoxicity mediated by NK cells but also interferon (IFN)-γ production by NK cells after exposure to interleukin (IL)-2, IL-12, IL-15, or CD16 cross-linking. (pubmedcentralcanada.ca)
  • Determining the genotype is important from a clinical point of view, as such a feature contributes in determining the potential response to the therapy based on the combination of alpha interferon with ribavirin, which is currently the most used treatment. (justia.com)
  • gene
  • To date, several host- and virus-related factors have been implicated in the progression to MCII, such as the virus-induced expansion of selected subsets of B-cell clones expressing discrete immunoglobulin variable (IgV) gene subfamilies, the involvement of complement factors and the specific role of some HCV proteins. (mdpi.com)
  • polypeptide
  • The two 5-proximal ORFs encode the replicases, two internal ORFs encode movement proteins, and the 3-proximal ORF encodes a polypeptide (p37) which plays a dual role as capsid protein and as suppressor of RNA silencing. (jove.com)
  • bind
  • In line, HBsAg preferentially bound to TLR9-triggered pDC demonstrating that once pDC are able to bind HBV/HBsAg, the virus exerts its immune regulatory effect. (jove.com)
  • complexes
  • In particular, about 60% of HCV-infected patients present cold-precipitable (cryoprecipitable) and noncryoprecipitable immune complexes that could be associated with the clinical onset of type II mixed cryoglobulinemia (MCII) [ 2 ]. (mdpi.com)
  • allosteric
  • Here, we aimed to provide some insight into the inhibitor binding mode and allosteric regulation mechanism of HCV NS3/4A protein by using computational methods. (jove.com)
  • specific
  • Following immunization, rhesus macaques mounted strong HCV-specific T cell immune responses strikingly similar to those reported in patients who have cleared the virus on their own. (wikipedia.org)
  • virus
  • citation needed] Scientists can study the immune system's response to the virus in horses and study if vaccine candidates have any effect. (wikipedia.org)
  • Human Leukocyte Antigen (HLA) Class I Down-Regulation by Human Immunodeficiency Virus Type 1 Negative Factor (HIV-1 Nef): What Might We Learn From Natural Sequence Variants? (mdpi.com)
  • Little is known about how this virus is able to persist or whether this persistence might be because of its ability to alter the early innate immune response. (pubmedcentralcanada.ca)
  • A number of possibilities have been proposed to explain why people infected with HCV fail to resolve this virus despite generating both cellular and humoral immune responses. (pubmedcentralcanada.ca)
  • interaction
  • Further residue interaction network analysis suggests the communication of the domain-domain interface play an important role in the transition from closed to open conformation of HCV NS3/4A protein. (jove.com)
  • human
  • Therefore, HBV and HBV-derived proteins were examined for their effect on human pDC in vitro. (jove.com)
  • expression
  • The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins such as RP105, a recently identified homolog of TLR4. (blogspot.com)
  • time
  • Cell-free culture supernatants, protein lysates and total RNA were harvested at various time points post-transfection to assess HCV growth. (jove.com)