• Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • Dichloroacetate (20 mM) did not reduce growth of non-cancerous cells but caused significant decrease in cancer cell proliferation ( P =0.009), which was associated with apoptosis and G 2 phase cell-cycle arrest. (dcaguide.org)
  • In fact, many studies have demonstrated the oleuropein and its derivative have proved pharmacological activity against proliferation of cancer cells and several tumor cell lines by different mechanisms such arrest cell cycle and cause apoptosis in cancerous cells, by modulation of miRNA expression and upregulation and downregulation of several genes. (springer.com)
  • This study investigated the effect of acylated synthetic ghrelin (AG) on the survival and proliferation of human chemosensitive ovarian cancer cells (A2780) and explored some mechanisms of action with a focus on the p53 apoptotic pathway and PI3K/Akt and NF- κ B survival pathways. (hindawi.com)
  • However, while AG treatment had no effect on control cell viability, it significantly increased cell viability and proliferation and inhibited cell death in Cis-treated cells. (hindawi.com)
  • Among them and over decades of research, it was shown that the PI3K/Akt/mTOR pathway is one of the most frequently hyperactive pathways in OC as well as other types of solid tumors and is associated with increased cell growth, proliferation, migration, protein synthesis, survival, and chemotherapy resistance [ 7 - 9 ]. (hindawi.com)
  • While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
  • For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
  • β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)
  • Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
  • We treated TMZ-resistant cells U251-TMZ and T98G-TMZ to normoxia or hypoxia and silenced NUPR1 in hypoxia-treated U251-TMZ and T98G-TMZ cells to assess cell viability, proliferation, apoptosis, LC3-II/LC3-I and p62 expressions, and autophagic flux under different concentrations of TMZ. (techscience.com)
  • Bosutinib is a potent inhibitor of CML cell proliferation in vitro and has demonstrated promising activity in CML patients resistant or intolerant to IM as well as in newly diagnosed patients with chronic phase CML (CML-CP). (mdm2-receptor.com)
  • Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. (natprobiotech.com)
  • TGF B as a tumor promoter TGF B acts as tumor suppressor in normal epithelium, it inhibits cell proliferation and induces apoptosis. (mirnaarray.com)
  • Encapsulated cells maintained high viability and displayed cell type-specific differences in morphology, proliferation, metabolic activity, ultrastructure, and overall microsphere size distribution and bulk stiffness. (nsf.gov)
  • Administration of insulin increased colon cancer cell proliferation and polyp formation in Azoxymethane (AOM)-induced cancer model ( 19 , 20 ). (amegroups.org)
  • The activation of PI3K/Akt pathway can increase cell survival, cell growth and proliferation ( 22-24 ). (amegroups.org)
  • We have shown that colorectal cancer cell lines defective in DNA MMR exhibit an increased sensitivity to both camptothecin, a topoisomerase I inhibitor, and etoposide, a topoisomerase II inhibitor. (aacrjournals.org)
  • Our results also indicate that neither p53 status, nor cell cycle alterations correlate with the sensitivity of colorectal cancer cells to topoisomerase inhibitors. (aacrjournals.org)
  • Our aim was to determine if switching metabolism from glycolysis towards mitochondrial respiration would reduce growth preferentially in colorectal cancer cells over normal cells, and to examine the underlying mechanisms. (dcaguide.org)
  • Representative colorectal cancer and non-cancerous cell lines were treated with dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase. (dcaguide.org)
  • Pyruvate dehydrogenase kinase inhibition attenuates glycolysis and facilitates mitochondrial oxidative phosphorylation, leading to reduced growth of colorectal cancer cells but not of non-cancerous cells. (dcaguide.org)
  • In this review, we will discuss the mechanisms according to which T2D induces cellular senescence and the role of type 2 diabetes-induced cellular senescence in the pathogenesis and progression of colorectal cancer. (frontiersin.org)
  • In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)
  • Colorectal cancer cell lines were treated with different doses of cetuximab to create ten test groups, which were then incubated for 24 and 48 hours. (natprobiotech.com)
  • Colorectal cancer cells are resistant to anti-EGFR monoclonal antibody through adapted autophagy. (natprobiotech.com)
  • Anti-EGFR antibody sensitizes colorectal cancer stem-like cells to Fluorouracil-induced apoptosis by affecting autophagy. (natprobiotech.com)
  • MCF7 and MDA-MB-231 cell lines were used to create breast cancer microspheres and the HT29 cell line and cells from a stage II patient-derived xenograft (PDX) were encapsulated to produce colorectal cancer (CRC) microspheres. (nsf.gov)
  • Autophagy is an evolutionary conservative process that maintains cellular homeostasis and viability [ 1 ]. (aging-us.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • Hydrogels prepared with 3.25% and 3.5% (w/v) SA and 4% (w/v) GL showed higher printability and cell viability. (nsf.gov)
  • They therefore selected and synthesized, using our chemical and pharmaceutical skills, a series of molecules on which we performed proteomics experiments (DARTs) to confirm the selected target, choosing some cell lines resistant to chemotherapy such as mitoxantrone (including HepG2, MCF7-transfected and HT29). (ebris.eu)
  • It is involved in cell death, growth, and differentiation as well as tissue repair by tissue remodeling/wound healing and ECM assembly [ 5 ] . (encyclopedia.pub)
  • Abaza L, Talorete TP, Yamada P, Kurita Y, Zarrouk M, Isoda H (2007) Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. (springer.com)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. (cancerindex.org)
  • Role of p27(Kip1) in human intestinal cell differentiation. (natprobiotech.com)
  • circRNAs are covalently closed-loop structures that are usually very stable and not easily degraded in the cellular microenvironment [ 6 ]. (hindawi.com)
  • Given that cancer cells and astrocytes in glioblastomas coexist in a hypoxic microenvironment, we examined whether astrocytes affect the adaptation of glioblastoma cells to hypoxia. (cancerindex.org)
  • Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
  • 11 Chemoresistance, either acquired or intrinsic, is associated with a complicated and multifactorial process, including decreases of intracellular drugs, changes of drug targets, aberrant regulation of cell survival or death, and crosstalks between tumor microenvironment and cancer cells. (dovepress.com)
  • 3D bioprinting improves orientation of in vitro tumor models by offering layer by layer positioning of cancer cells and cancer associated fibroblasts (CAFs) which can replicate tumor microenvironment. (nsf.gov)
  • Current commercial techniques, however, rely heavily on self-aggregation of dissociated cells and are unable to replicate key features of the native tumor microenvironment, particularly due to a lack of control over extracellular matrix components and heterogeneity in shape, size, and aggregate forming tendencies. (nsf.gov)
  • In this review, we summarize the current status and emerging trends in nanomedicine-based strategies targeting cancer immune evasion and modulating the immunosuppressive tumor microenvironment, including the inhibition of immunosuppressive cells in the tumor area, the activation of dendritic cells and the stimulation of the specific antitumor T-cell response. (unav.edu)
  • Multidrug resistance caused by the overexpression of ABC transporter proteins in cancer cells remains a major obstacle limiting chemotherapy efficacy. (spandidos-publications.com)
  • This proadifen-MTX synergism was also mediated by the inhibition of various cellular proteins engaged in apoptosis, including Mc-1, Bcl-xL, survivin and activation of procaspase-3. (spandidos-publications.com)
  • It was previously reported that proadifen (SKF-525A), a well-known cytochrome P450 monooxygenase inhibitor, not only has anti-proliferative potential in some cancer cell lines, but it is also able to inhibit BCRP and MRP1 transporter proteins ( 8 ). (spandidos-publications.com)
  • Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
  • Through the Molecular Docking studies, the research team of the Oncotherapy Project of the Ebris Foundation designed a library of potentially active molecules on a protein belonging to the family of MDR (Multi Drug Resistance proteins) known to be expressed in numerous resistant tumor forms. (ebris.eu)
  • AbstractHerpesviruses are known to encode a number of inhibitors of host cell death, including Rip Homotypic Interaction Motif (RHIM)-containing proteins. (howhelp.org)
  • Rip homotypic interaction motifs (RHIMs) are found in host proteins that can signal for programmed cell death and in viral proteins that can prevent it. (howhelp.org)
  • For the other hand, down regulation of Id proteins in epithelial cells is because of activated SMAD3 that induces activating transcription factor expression then together with ATF directly represses the Id promoter. (mirnaarray.com)
  • Most of the identified peptides originated from bacterial cell surface proteins. (calbinotox.fr)
  • At 50 μg mL -1 , the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. (scialert.net)
  • These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies. (scialert.net)
  • The largest apoptotic effect was evident in metastatic LoVo cells, in which DCA induced up to a ten-fold increase in apoptotic cell counts after 48 h. (dcaguide.org)
  • In our previous study, we demonstrated the anti-proliferative and pro-apoptotic activity of proadifen in the HT-29 cancer cell line ( 9 ). (spandidos-publications.com)
  • Treatment of control ERas cells with PD0325901 for 24 h results in mitochondria damage and apoptotic death of a part of cellular population. (aging-us.com)
  • Senescent cells are unable to form the autophagolysosomes and to remove the damaged mitochondria resulting in apoptotic death. (aging-us.com)
  • Essential oil prepared by distillation of the gum resin traditionally used for aromatic therapy has also been shown to have tumor cell-specific anti-proliferative and pro-apoptotic activities. (biomedcentral.com)
  • Asgharzade S, Sheikhshabani SH, Ghasempour E, Heidari R, Rahmati S, Mohammadi M, Jazaeri A, Amini-Farsani Z (2020) The effect of oleuropein on apoptotic pathway regulators in breast cancer cells. (springer.com)
  • The gene is preferentially expressed by immature dendritic cells and memory T cells. (cancerindex.org)
  • Quantitative proteomics analysis revealed compromised chicken dendritic cells function at early stage of very virulent infectious Bursal disease virus infection. (upm.edu.my)
  • Dendritic cell frequency increased in PP regardless of the TiO 2 treatment, while regulatory T cells involved in dampening inflammatory responses decreased with E171 only, an effect still observed after 100 days of treatment. (nature.com)
  • Because mitoxantrone (MTX) is a strong BCRP substrate and is often used in the treatment of leukemia, we investigated the effect of 24 h proadifen pre-treatment on the cytotoxicity of MTX in leukemic cell lines that are sensitive to MTX (HL-60) and MTX-resistant ABCG2-overexpressing subclone (cBCRP). (spandidos-publications.com)
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • Subsequently, in-cell assays were performed to test the inhibitory potency of our compounds by evaluating their cytotoxicity in co-administration with the reference chemotherapeutic agents. (ebris.eu)
  • Evaluating cytotoxicity potency of glucomoringin from the seeds of Moringa oleifera lam against prostate cancer cell line, PC-3. (upm.edu.my)
  • The extract also showed a dose dependent growth inhibition of all tested cell lines, IC 50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL -1 , respectively. (scialert.net)
  • We show for the first time that proadifen is able to enhance the cytotoxic properties of MTX in cBCRP cells, particularly through the inhibition of BCRP expression and activity. (spandidos-publications.com)
  • Moreover, we revealed the ability of proadifen to increase the intracellular hypericin content in HT-29 cells most likely via the inhibition of BCRP and MRP1 transporters ( 8 ). (spandidos-publications.com)
  • We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
  • Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
  • Mutation of the ORF20 RHIM renders the virus incapable of efficient spread in ZBP1-expressing HT-29 cells, an effect which can be reversed by the inhibition of caspases. (howhelp.org)
  • BDNF/TrkB signaling protects HT-29 human colon cancer cells from EGFR inhibition. (natprobiotech.com)
  • Other CDK in hibitory responses, observed in various cell kinds following exposure to TGF B, are inhibition of CDK4 expression and down regulation of CDC25A expression. (mirnaarray.com)
  • Our previous protocol to differentiate human induced pluripotent stem cells (iPSCs) into skin-derived precursor cells (SKPs) as a source of dermal stem cells employs mouse fibroblasts as feeder cells and is therefore unsuitable for clinical use. (bvsalud.org)
  • In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
  • To determine whether the PI3K pathway W ww During MCMV infection activated in our system, the cell lysates infected with MCMV in fibroblasts by Western blot with a specific agency for ex-Akt phosphorylated at serine 473 analyzed. (microrna1.com)
  • Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
  • Cell survival was determined 7, 8, or 9 days after RT by the sulforhodamine B test. (biomedcentral.com)
  • Thanks to its multiple roles, TG2 exerts various biological functions depending on the stimulus, leading to cell death or survival and tissue repair. (encyclopedia.pub)
  • This review aims to summarize the recent knowledge on the mechanisms activated by TG2 to regulate cell death/survival and fibrosis in the tissue repair process. (encyclopedia.pub)
  • In conclusion, the findings of this study show that AG promotes cell survival of the OC cells and renders them resistat to Cis therapy, an effect that is mediated by the activation of PI3K/Akt/mTOR and activation of NF- κ B, and requires GHS-R1a. (hindawi.com)
  • This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
  • In this study, we demonstrated that NSCLC-derived CAFs were innately resistant to cisplatin treatment and CAFs-conditioned medium significantly promoted the survival rate of NSCLC cells after cisplatin treatment. (dovepress.com)
  • PI3K confinement Lich Is particularly important for the growth and survival of the cell with embroidery and is an important means of cellular Transformation FF Ren, another condition Cilomilast SB-207499 known to induce the expression of NKG2D ligands for rdern. (microrna1.com)
  • PI3K class I, which is essential in the regulation of cell survival, metabolism, apoptosis and cell cycle of the heterodimeric class I molecules of a catalyst composition PI3Ks regulatory subunit and are classified in class IA or IB PI3K class. (microrna1.com)
  • The glycolytic phenotype offers growth advantages to cancer cells by resisting apoptosis, and facilitating tumour spread and metastasis (Yeluri et al, 2009). (dcaguide.org)
  • Immunoblotting, reporter assays, quantitative RT-PCR, and chromatin immunoprecipitation were performed to evaluate HIF-1 signaling in glioblastoma cells. (cancerindex.org)
  • In particular, a protocol for the preparation of 3D cell cultures in micro-fluidics was developed in the laboratories of the Ebris Foundation, which induces the production of extracellular matrix (ECM) resulting from an active interaction between tumor cells and stromal cells, mimicking what happens in vivo. (ebris.eu)
  • During tumor development, stromal cells are co-opted to the tumor milieu and provide favorable conditions for the tumor. (cancerindex.org)
  • A prostatic stromal myofibroblast cell line (WPMY-1) was co-incubated with testosterone (1 µM) and different concentrations of capsaicin (10-100 µM) for 24 and 48 h. (techscience.com)
  • Consistent with that obtaining are our data showing that retromer knockdown has no detectable impact on RI trafficking or Smad phosphorylation nevertheless is critical for suitable mem brane localization of RII in polarized epithelial cells. (mirnaarray.com)
  • In fact, we found, as Yu et al inhibited PI3K In various epithelial cells and h Hematopoietic Ethical hh M Usen ethical and legal persons. (microrna1.com)
  • Movement adults TLR5 h in both epithelial Matopoetische ethical behavior and expression h differ, these cells greatly in their exposure to natural disasters TLR5 ligand flagellin. (microrna1.com)
  • Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. (calbinotox.fr)
  • We may conclude that CTX induces apoptosis and autophagy in HT-29 KRK cells through raising the expression of p21, p27 , and p57 genes, as well as ATG4A, LC3A , and BECN1 genes. (natprobiotech.com)
  • In vitro simulated digestion allowed to conclude that XOS were resistant to gastric conditions but phenolics were not. (ucp.pt)
  • VZV is a highly cell-associated virus and does not release cell-free virions into culture [9], necessitating cell-associated propagation of the virus in vitro. (howhelp.org)
  • In vitro HaCaT cell photoaging model was used to examine the photoprotection effect of DOP. (bvsalud.org)
  • A key regulator of cellular metabolism is pyruvate dehydrogenase (PDH). (dcaguide.org)
  • Other partners of the intracellular metabolism of gemcitabine in relation to the cell cycle effects and DNA repair could be more responsible for the radiosensitising effect than dCK activity. (biomedcentral.com)
  • Senescence is a stress-response cellular state characterised by proliferative arrest but active metabolism ( 7 ). (frontiersin.org)
  • The degree of radiosensitisation was also cell line dependent and seemed to correlate with the sensitivity of the cell line to the cytotoxic effect of dFdC. (biomedcentral.com)
  • The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
  • Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
  • Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
  • High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
  • This study tested insulin-caused resistance to oxaliplatin via activation of PI3K/Akt pathway in HT29 cells. (amegroups.org)
  • Insulin decreased drug efficacy of oxaliplatin in HT29 cells, which could be mediated by the activation of the PI3K/Akt pathway. (amegroups.org)
  • As the activation of PI3K/Akt signal pathway increases the resistance of several cancer cell lines such ovarian, lung cancer to chemotherapeutic drugs ( 7 , 8 ), it is possible that PI3K/Akt may also play a role in the poor prognosis of obesity-associated colon cancer. (amegroups.org)
  • Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
  • Astrocytes augmented HIF-1α expression in glioblastoma cells under hypoxia. (cancerindex.org)
  • The expression of HIF-1 downstream genes, cancer colony formation, and Matrigel invasion of glioblastoma cells were stimulated by conditioned medium from astrocytes pre-exposed to hypoxia. (cancerindex.org)
  • CCL20 was secreted in a hypoxia-dependent manner from astrocytes and busted the hypoxic induction of HIF-1α in glioblastoma cells. (cancerindex.org)
  • BCRP expression was detected in several different solid tumours ( 1 - 5 ), as well as in malignant hematopoietic and lymphoid cells ( 6 ). (spandidos-publications.com)
  • According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
  • Hypoxia stimulates cancer cells to acquire a more malignant phenotype via activation of hypoxia-inducible factor 1 (HIF-1). (cancerindex.org)
  • Oral squamous cell carcinoma (OSCC) is a common malignant tumor. (techscience.com)
  • In addition to the realization of cell cultures in 3D, the collected tissues were fragmented and stored in cryogenic tubes in order to set up a bio-bank of tissues to be used both in view of the application of protocols to create organoid cultures from frozen tissues and through flash frozen technique, for genomic and proteomic sequencing. (ebris.eu)
  • Furthermore, nanomedicine provides a promising opportunity to enhance the efficacy of cancer immunotherapy by improving the delivery, retention and release of immunostimulatory agents in targeted cells and tumor tissues. (unav.edu)
  • In some cases, cisplatin+AG-treated cells were pre-incubated either with [D-Lys3]-GHRP-6, a ghrelin receptor antagonist, or with LY294002, a PI3K inhibitor. (hindawi.com)
  • We have identified a novel viral RHIM in the VZV capsid triplex protein, open reading frame (ORF) 20, that acts as a host cell death inhibitor. (howhelp.org)
  • TGF B induces the expression of the CDK inhibitor p15INK4B inside a assortment of cell sorts. (mirnaarray.com)
  • 4 As the primary type of lung cancer, non-small cell lung cancer (NSCLC) exhibits multiple histological types, such as bronchoalveolar carcinoma, large cell carcinoma, squamous cell carcinoma, and adenocarcinoma. (dovepress.com)
  • Although curcumin has shown significant efficacy in cell culture studies, it has shown limited efficacy in clinical studies when administered in conventional oral formulations. (researchgate.net)
  • It has been reported to reduce growth of these cancer cells mainly by reducing inhibitory phosphorylation of PDH, thereby promoting mitochondrial oxidative phosphorylation and inducing apoptosis through mitochondrial, NFAT-Kv 1.5, and p53 upregulated modulator of apoptosis (PUMA)-mediated pathways. (dcaguide.org)
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • Members of the Herpesviridae family, Herpes simplex virus (HSV) -1 and human and murine cytomegalovirus (HCMV/MCMV), are masters at manipulating host cell death pathways such as apoptosis and necroptosis, in order to successfully spread and establish latency [1-3]. (howhelp.org)
  • β-Catenin was initially discovered in the early 1990s as a component of a mammalian cell adhesion complex: a protein responsible for cytoplasmatic anchoring of cadherins. (wikipedia.org)
  • NMR metabolomics for evaluating passage number and harvesting effects on mammalian cell metabolome. (upm.edu.my)
  • Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
  • Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
  • Given that autophagy is initiated upon suppressed mTORC1 activity, a protective autophagy in Ras-transformed cells with a high level of mTORC1 activity appears to be mTORC1-independent [ 20 , 21 ]. (aging-us.com)
  • One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
  • This study investigated the mechanism of NUPR1 in TMZ resistance in hypoxia-treated glioma cells and its mechanism in modulating autophagy. (techscience.com)
  • We found that hypoxia upregulated NUPR1 expression and autophagy while NUPR1 silencing suppressed hypoxia-induced TMZ resistance and autophagy in glioma cells. (techscience.com)
  • For autophagy flux detection, the adenovirus loaded with mRFP-GFP-LC3 was introduced into cells. (bvsalud.org)
  • RESULTS: The results showed that DOP achieve photoaging protection by inhibiting oxidative stress, alleviating cell cycle arrest and apoptosis, and enhancing autophagy flux in photoaged HaCaT cells. (bvsalud.org)
  • Interestingly, pre-incubating the cells with either [D-Lys3]-GHRP-6 or LY294002 completely abolished the above-mentioned effect of AG in both control and Cis-treated cells. (hindawi.com)
  • Virus titers in the supernatant of the infected cells for 24 hours in the presence or absence of LY294002 was not collected substantially different, indicating that the lack of expression of RAE Fl step Che. (microrna1.com)
  • When the cells were infected in the presence of LY294002, the phosphorylated form of Akt was no longer detectable. (microrna1.com)
  • Over the years, characterization and functional studies have revealed the complexity of ROS as signaling molecules that regulate various physiological cellular responses or whose levels are altered in various diseases. (degruyter.com)
  • A connectivity score between two perturbations quantifies the similarity of the cellular responses evoked by these perturbations. (clue.io)
  • In all TiO 2 -treated rats, stimulation of immune cells isolated from PP showed a decrease in Thelper (Th)-1 IFN-γ secretion, while splenic Th1/Th17 inflammatory responses sharply increased. (nature.com)
  • On the other hand,our data showing that resistance to these drugs can be achieved by the functional complementation of hMLH1 in an hMLH1-defective cell line have allowed us to establish that MMR is a critical determinant for chemosensitivity. (aacrjournals.org)
  • Because MTX is a preferential and strong substrate of BCRP, MTX-sensitive HL-60 cell line and an MTX-resistant ABCG2-overexpressing subclone (here referred to as cBCRP) were chosen as the experimental models. (spandidos-publications.com)
  • Human promyelocytic leukemia cell line HL-60 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and its ABCG2-overexpressing subclone (here referred to as cBCRP) was kindly provided by Dr Balazs Sarkadi (Membrane Research Group, Hungarian Academy of Sciences, Budapest, Hungary) ( 10 ). (spandidos-publications.com)
  • When running GUTC, incorporates cell-line information to match query data against matching cell types in Touchstone. (clue.io)
  • When running GUTC, does not incorporate cell-line information when querying the data against Touchstone signatures. (clue.io)
  • All data is from the Cancer Cell Line Encyclopedia resource. (clue.io)
  • Several compounds have been shown to inhibit the efflux activity of ABC transporters, thereby increasing intracellular drug accumulation and sensitising cancer cells to therapy. (spandidos-publications.com)
  • Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
  • Like the human cellular RHIMs in RIPK1 and RIPK3 that stabilise the necrosome in TNF-induced necroptosis, and the viral RHIM in M45 from murine cytomegalovirus that inhibits cell death, the ORF20 RHIM is capable of forming fibrillar functional amyloid complexes. (howhelp.org)
  • Permeability of XOS across the intestinal cell layer decreased from 45% to 7.9 and 15.7%, after encapsulation in alginate or chitosan MPs, respectively. (ucp.pt)
  • mRNA of ghrelin receptors(GHS-R1a and GHS-R1b), as well as, protein levels of GHS-R1a, were expressed abundantly in A2780 cells. (hindawi.com)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • 1) Cell receptors for rotavirus are being identified and characterized by a variety of biochemical and immunological approaches. (stanford.edu)
  • Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
  • The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
  • Despite the current revolutionary advances in the field of cancer immunotherapy, clinical response in breast cancer is frequently below expectations, in part due to various mechanisms of cancer immune escape that produce tumor variants that are resistant to treatment. (unav.edu)
  • The most striking G 2 arrest was evident in well-differentiated HT29 cells, in which DCA caused an eight-fold increase in cells in G 2 phase after 48 h. (dcaguide.org)
  • In both control and Cis-treated cells, AG treatment significantly increased PI3K/Akt/mTOR signaling and enhanced the nuclear accumulation of NF- κ B. Concomitantly, in both control and Cis-treated cells, AG significantly lowered the protein levels of p53, p-p53 (Ser16), PUMA, cytochrome C, and cleaved caspase-3. (hindawi.com)
  • As positive controls the whole cell lysates of cells 20 ff valley KK or K Calf serum cells stably express the catalytic subunit of PI3K with an fa mutation treated Brands H1047R PI3K is constitutively active. (microrna1.com)
  • Gesamtverm Gens MCMV infection PI3K/Akt our data, based on data with HCMV and that this activation to induce signaling through PI3K p110a RAE RAE first required significant induction in cells infected with MCMV There are three classes obtained enzymes in the superfamily of PI3K class I, II and III. (microrna1.com)
  • There will always play the PI3K catalytic subunits r No redundant in the regulation of cell biology. (microrna1.com)
  • Therefore, it is not surprising that it would, in response to signaling flagellin distinguish between these two types of cells, and the involvement of kinases downstream Rts mediators such as PI3K RTS is a difference which can be achieved. (microrna1.com)
  • Dichloroacetate reduced lactate levels in growth media and induced dephosphorylation of E1 α subunit of pyruvate dehydrogenase complex in all cell lines, but the intrinsic mitochondrial membrane potential was reduced in only cancer cells ( P =0.04). (dcaguide.org)
  • In the present study, the relation between deoxycytidine kinase (dCK) activity and the radiosensitising effect of gemcitabine was investigated in eight different human tumour cell lines. (biomedcentral.com)
  • A clear concentration-dependent radiosensitising effect of dFdC was observed in all cell lines. (biomedcentral.com)
  • The dCK activity of our cell lines varied considerably and differed up to three fold from 5 to 15 pmol/h/mg protein between the tested cell lines. (biomedcentral.com)
  • The leaf extracts and its derivative compounds have shown potent anticancer effects against different cell lines in different organs. (springer.com)
  • In this work, olive leaf waste was valorized by silver nitrate to produce silver nanoparticles (OLAgNPs), which exhibited various biological, antioxidant, anticancer activities against three cancer cell lines, and antimicrobial activity against multi-drug resistant (MDR) bacteria and fungi. (mdpi.com)
  • See a heatmap of connections between individual perturbagens in cell lines and all other perturbagens used for the P100 assay or the GCP assay. (clue.io)
  • The data were then normalized such that the expression values were comparable across cell lines, by minimizing technical variation and equalizing their distributions (for details of the normalization, see LISS and QNORM entries in the Connectopedia glossary ). (clue.io)
  • More recently, cellular senescence has been considered as an additional cause of age-related tumorigenesis. (frontiersin.org)
  • However, cellular senescence is not exclusive to ageing. (frontiersin.org)
  • Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
  • Also, chitosan was modified with a fluorescent probe for cellular uptake studies. (ucp.pt)
  • Modification of chitosan MPs with Cyanine5.5 for cellular uptake studies did not affect the biocompatibility with intestinal cells, and confocal microscopy analysis confirmed the integrity of these cells tight junctions. (ucp.pt)
  • Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
  • Paclitaxel enhances the effects of the anti-epidermal growth factor receptor monoclonal antibody ImClone C225 in mice with metastatic human bladder transitional cell carcinoma. (natprobiotech.com)
  • Mutation of cell-cycle regulators biological and clinical implications for human neoplasia. (natprobiotech.com)
  • Human A2780 ovarian cancer cells were cultured with or without AG treatment in the presence or absence of cisplatin. (hindawi.com)
  • In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. (techscience.com)
  • Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. (techscience.com)
  • MPs were characterized for their size, polydispersity, zeta potential and total phenolics (TPC) association efficiency, and the simulation of gastrointestinal digestion was performed to evaluate the release profile of polyphenols, the antioxidant and antimicrobial activities, and permeability of phenolics and XOS across Caco-2/ HT29-MTX cell layer. (ucp.pt)
  • Chemoresistance is a significant barrier to the treatment and management of non-small cell lung cancer (NSCLC). (dovepress.com)
  • Additionally, CAFs-derived exosomes were taken up by NSCLC cells. (dovepress.com)
  • Moreover, exosomal miRNA-130a was transferred from CAFs to recipient NSCLC cells and knockdown of miRNA-130a reversed the effect of CAFs-derived exosomes during chemoresistance of NSCLC cells. (dovepress.com)
  • Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. (dovepress.com)
  • Aim of this study was to develop a sodium alginate -gelatin (SA-GL) hydrogel by optimizing rheological parameters to print non-small cell lung cancer (NSCLC) patient derived xenograft (PDX) cells and lung CAFs co-cultures. (nsf.gov)