• Above average presence of asparagine synthetase in certain leukemia strains has been linked to be a significant contributing factor of chemotherapy resistance, particularly to the chemotherapy drug, L-asparaginase. (wikipedia.org)
  • We present our study on the efficacy and phenotypic impact of compensatory evolution in Escherichia coli strains carrying multiple resistance mutations. (elifesciences.org)
  • Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous strains in terms of serotypes, adherence patterns and the presence of novel virulence factors. (utmb.edu)
  • Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a α/β, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. (utmb.edu)
  • Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. (rhea-db.org)
  • To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by stepwise selection for resistance to increasing concentrations of levofloxacin. (omicsdi.org)
  • On the basis of the sequence information, we performed DNA amplification for sequencing and single-strand conformation polymorphism analysis to examine the presumed quinolone resistance regions of gyrA and gyrB from reference strains (n = 4) and clinical isolates (n = 55). (omicsdi.org)
  • In China, the presence of the qnr gene in the clinical isolates of Enterobacteriaceae has been reported, but this transmissible quinolone resistance gene has not been detected in strains isolated singly from pediatric patients. (biomedcentral.com)
  • The study found that 19 ciprofloxacin-resistant clinical isolates of E. coli and K. pneumoniae were positive for the qnr gene, and most of the qnr positive strains were ESBL producers. (biomedcentral.com)
  • This report on transferable fluoroquinolone resistance due to the qnr gene among E. coli and K. pneumoniae strains indicated that plasmid-mediated quinolone resistance has emerged in pediatric patients in China. (biomedcentral.com)
  • Due to increased resistance, there is an exigency to develop novel therapeutic targets to combat these resistance strains. (microbiologyresearch.org)
  • Due to the appearance of the highly virulent strain of E. coli of serotype O157:H7 in the US and Canada in the 1980's, and subsequently in other Latin American countries, there is an increase need for accurate testing for this and other pathogenic E. coli strains, substantially enhancing detection of virulent strains and, therefore, facilitating identification of sporadic E. coli infections and outbreaks. (eurekaselect.com)
  • The emergence and evolution of pathogenic Escherichia coli strains associated with diarrheal diseases have become a topic of active investigation in recent years due to the emergence of more virulent strains and the association of new serotypes with disease. (eurekaselect.com)
  • The relative contribution of recombination events in the generation of new categories of pathogenic E. coli varies among the E. coli population, and it is represented by the wide variety of mobile elements found in different diarrheal strains (e.g. pathogenicity islands, phages, transposons, pathoadaptive mutations, etc). (eurekaselect.com)
  • In addition, factors underlying the emergence of enteroaggregative and atypical enteropathogenic E. coli strains associated with persistent diarrhea are unknown. (eurekaselect.com)
  • Horizontal transfer of genetic elements that affect virulence of diarrheagenic E. coli strains and changes in global agricultural processes, as well as movement of humans and animals, may contribute to the complex natural history of diarrheagenic E. coli. (eurekaselect.com)
  • Ampicillin is a second-generation penicillin that is active against many strains of Escherichia coli, Proteus mirabilis, Salmonella, Shigella, and Haemophilus influenzae. (medscape.com)
  • Of EAEC strains from Nigeria, 10 independent antimicrobial-resistant isolates belonged to the multilocus sequence type 69 clonal complex, to which uropathogenic E. coli clonal group A belongs. (cdc.gov)
  • Markers of well-characterized aggregative adherence plasmids, present in typical EAEC strains, are aggregative adherence regulator gene aggR , anti-aggregative protein or dispersin gene aap, and empiric plasmid probe (CVD432) that represents part of the aat secretion system operon ( 6 ). (cdc.gov)
  • The aim of this study was to characterise the molecular mechanism of resistance in the clinical isolates of Enterobacteriaceae causing bacteremia and showing resistance to β-lactams, including carbapenems. (omicsonline.org)
  • Isolates of E.coli (n=42) and K. pneumoniae (n=134) from blood culture collected during 2013-2015 were screened for carbapenemase production by using carba NP test and the presence of carbapenem resistant genes (KPC, IMP, VIM, NDM and OXA- 48 like). (omicsonline.org)
  • Replating of those first-step mutants selected for mutants with high levels of resistance which harbored gyrA mutations similar to those found among clinical FQ-resistant isolates. (omicsdi.org)
  • The gyrA and gyrB sequence information will facilitate analysis of the mechanisms of resistance to drugs which target the gyrase and the implementation of rapid strategies for the estimation of FQ susceptibility in clinical M. tuberculosis isolates. (omicsdi.org)
  • Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens. (omicsdi.org)
  • DNA gyrase mutations are a major cause of quinolone resistance in Mycobacterium tuberculosis We therefore conducted the first comprehensive study to determine the diversity of gyrase mutations in pre-extensively drug-resistant (pre-XDR) (n = 71) and extensively drug-resistant (XDR) (n = 30) Thai clinical tuberculosis (TB) isolates. (omicsdi.org)
  • This study therefore aimed to investigate the presence of the qnr gene in clinical isolates of E. coli and K. pneumoniae from pediatric patients in China. (biomedcentral.com)
  • A total 213 of non-repetitive clinical isolates resistant to ciprofloxacin from E. coli and K. pneumoniae were collected from hospitalized patients at five children's hospital in Beijing, Shanghai, Guangzhou, and Chongqing. (biomedcentral.com)
  • The isolates were screened for the plasmid-mediated quinolone resistance genes of qnrA , qnrB , and qnrS by PCR. (biomedcentral.com)
  • Therefore, the objective of this study was to screen for the presence of the qnr gene in clinical ciprofloxacin-resistant isolates of Escherichia coli and Klebsiella pneumoniae from pediatric patients in China. (biomedcentral.com)
  • Three hundred thirty-five Escherichia coli and 392 Klebsiella pneumoniae non-replicate clinical isolates were collected from five children's hospitals located in Beijing, Shanghai, Guangzhou, and Chongqing from January 2005 to December 2006. (biomedcentral.com)
  • The screening showed 146 Escherichia coli and 67 Klebsiella pneumoniae isolates were ciprofloxacin-resistant. (biomedcentral.com)
  • However, a significant risk exists that infections caused by highly virulent E. coli isolates, such as the enterohemorrhagic E. coli O157:H7, develop into serious and potentially lifethreatening complications, such as hemolytic uremic syndrome. (eurekaselect.com)
  • Methicillin resistance has become more common among community isolates of S. aureus . (msdmanuals.com)
  • The high prevalence of ertapenem nonsusceptibility at a tertiary care hospital in Egypt was predominantly attributed to K. pneumoniae carbapenemase-mediated resistance mechanisms in K. pneumoniae isolates. (who.int)
  • We describe the clinical and microbiologic features associated with an ertapenem-resistant E. coli isolate that had reduced susceptibility to imipenem after in vivo treatment with imipenem/cilastatin and provide a detailed molecular analysis of the antimicrobial drug resistance mechanisms. (cdc.gov)
  • Microbial biofilms contribute to virulence and resistance to antibiotics by shielding microbial cells from host defenses and antimicrobial drugs, respectively. (who.int)
  • The emergence of antimicrobial resistance severely threatens our ability to treat bacterial infections. (frontiersin.org)
  • Gene products that confer intrinsic resistance to antimicrobial agents may be explored for alternative antimicrobial therapies, by potentiating the efficacy of existing antimicrobials. (frontiersin.org)
  • Our results demonstrate that many gene products contribute to the intrinsic antimicrobial resistance of S. aureus . (frontiersin.org)
  • Knowledge of these intrinsic resistance determinants provides alternative targets for compounds that may potentiate the efficacy of existing antimicrobial agents against this important pathogen. (frontiersin.org)
  • Besides the ability of bacteria to acquire antimicrobial resistance via horizontal gene transfer or spontaneous mutations, they can also be intrinsically resistant to antimicrobials ( Cox and Wright, 2013 ). (frontiersin.org)
  • Intrinsic resistance to antimicrobials has traditionally been attributed to reduced permeability of the cell envelope, presence of inactivating enzymes or efflux pumps that can extrude the antimicrobial agents ( Cox and Wright, 2013 ). (frontiersin.org)
  • Cell culture research shows that apple cider vinegar has antimicrobial actions against Escherichiacoli , Staphylococcus aureus , and Candida albicans. (traceminerals.com)
  • The SENTRY antimicrobial surveillance programme on antimicrobial resistance was conducted across India. (omicsonline.org)
  • Researcher Co-investigator: i-sense2, IRC in Agile Early Warning Sensing Systems for Infectious Diseases and Antimicrobial Resistance: EPSRC IRC (£3.9M, October 2018 -September 2022). (ncl.ac.uk)
  • The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution. (dtu.dk)
  • These findings highlight the potential of a highly specific bivalent VHH-based feed additive in effectively delimiting pathogenic F4 + ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development. (dtu.dk)
  • UTI, although treatable, is now becoming increasingly tough to control because of rampant antimicrobial resistance in the Enterobacteriaceae family, particularly inEscherichia coli. (microbiologyresearch.org)
  • Review on Antimicrobial Resistance. (jonbarron.org)
  • Various antimicrobial agents are effective in the treatment of shigellosis, although options are becoming limited because of globally emerging drug resistance. (medscape.com)
  • TolC family proteins are ubiquitous among gram-negative bacteria, and the conserved apertures present a possible chemotherapeutic target in multidrug-resistant pathogens. (umsystem.edu)
  • Acute UTIs are characterized by frequent painful urination and abdominal or back pain and typically are treated with antibiotics, although multidrug resistance is a growing problem. (wustl.edu)
  • UTIs are becoming increasingly difficult to treat as multidrug resistance becomes more common. (wustl.edu)
  • We have demonstrated that drug-resistance frequently declines within 480 generations during exposure to an antibiotic-free environment. (elifesciences.org)
  • The increasing frequency of drug resistance has been attributed to a combination of antibiotic over-prescription and societal and technologic changes that affect the transmission of drug-resistant organisms. (ubc.ca)
  • New research avenues include research into vaccine design, mechanisms of drug resistance, and virulence determinants. (medscape.com)
  • Rapid sensitivity testing using bacteriophages considers the problem of drug resistance. (medscape.com)
  • Although Blr is not essential, previous studies have shown that the inactivation of the blr gene increased the sensitivity of bacteria to β-lactam antibiotics or their resistance to cell envelope stress. (pasteur.fr)
  • An increase in the frequency of antibiotic resistance in bacteria since the 1950s has been observed for all major classes of antibiotics used to treat a wide variety of respiratory illnesses, skin disorders, and sexually transmitted diseases. (icr.org)
  • Is this resistance the result of bacteria evolving new genes in response to the presence of antibiotics, or are antibiotic-resistant bacteria selected for in the environment by possessing antibiotic resistance genes beforehand? (icr.org)
  • Streptomyces-produced quinolone and coumarin antibiotics, such as novobiocin, interfere with a protein called gyrase that assists in the normal separation of double-stranded DNA during replication of DNA or transcription of messenger RNA. (icr.org)
  • Ribosomes, the structures where protein synthesis is catalyzed, are the targets of many other Streptomyces antibiotics such as spectinomycin, tetracycline, and streptomycin. (icr.org)
  • Many possess genes that encode proteins to neutralize the affects of antibiotics and prevent attacks on their cell machinery. (icr.org)
  • Carbapenemases are a versatile group of Ã�-lactamases that are characterised by their resistance to virtually all Ã�-lactam antibiotics including cephalosporins and carbapenems, complicating therapy and limiting treatment options. (omicsonline.org)
  • Earlier Postdoctoral positions involved investigating the translocation of toxins produced by E. coli , through the Gram negative outer membrane with a view to use these molecules as novel antibiotics. (ncl.ac.uk)
  • The ultimate goal of the research in our laboratory is the structure-based design of novel, therapeutically useful antibiotics and inhibitors of antibiotic-resistance mechanisms. (ubc.ca)
  • Here is the UM story: The UM team inserted a particularly unstable protein into Escherichia coli (E. coli), which forced the bacteria to either adapt by improving protein stability or die when exposed to antibiotics. (newenergyandfuel.com)
  • The nptII protein, neomycin phosphotransferase II, confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin, and was used as a selectable marker for transformed plant cells. (cbd.int)
  • The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
  • Escherichia coli are Gram-negative bacteria found as normal commensal flora in the gastrointestinal tract. (eurekaselect.com)
  • HAMP domain, Tar ligand binding domain homologue, Methyl-accepting chemotaxis protein (MCP) signalling domain [Interproscan]. (ntu.edu.sg)
  • Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae. (utmb.edu)
  • Quinolone resistance in Enterobacteriaceae results mainly from mutations in type II DNA topoisomerase genes and/or changes in the expression of outer membrane and efflux pumps. (biomedcentral.com)
  • There is also a lack of treatment options for bacteria groups including Acinetobacter and Enterobacteriaceae, such as Klebsiella and E.coli - which are commonly spread in hospitals and nursing homes and are often fatal. (jonbarron.org)
  • Hence, we conclude that CQD-CRISPR-Cas9-gRNA could be a novel therapeutic strategy for the eradication or treatment of antibiotic resistant pathogenic E. coli -based pathologies. (microbiologyresearch.org)
  • Alfredo G. Torres , " Pathogenic Escherichia coli in Latin America ", Bentham Science Publishers (2010). (eurekaselect.com)
  • At least six different categories of pathogenic E. coli causing enteric infections have been identified and further characterized. (eurekaselect.com)
  • Understanding the population structure of pathogenic E. coli is important, since it impacts the effectiveness of molecular epidemiological studies. (eurekaselect.com)
  • RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
  • Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli. (rhea-db.org)
  • Structural characterization of SpoIIIAB sporulation-essential protein in Bacillus subtilis. (ubc.ca)
  • Characterization of the two conformations adopted by the T3 SS inner-membrane protein PrgK. (ubc.ca)
  • In theory, the extent of fitness costs determines the long-term stability of resistance, and consequently, the rate by which the frequency of resistant bacteria decreases in an antibiotic-free environment. (elifesciences.org)
  • While acquired resistance has received considerable attention, relatively little is known of intrinsic resistance that allows bacteria to naturally withstand antimicrobials. (frontiersin.org)
  • To answer these questions a discussion of several factors involved in antibiotic resistance will show that resistance is a designed feature of pre-existing genes enabling bacteria to compete with the antibiotic producers in their environment. (icr.org)
  • Second, apple cider vinegar that's organic and unfiltered contains a healthful compound known as "mother," a combination of enzymes, proteins, and beneficial bacteria. (traceminerals.com)
  • Their research points to a key protein that bacteria use to latch onto the bladder and cause UTIs, according to scientists at Washington University School of Medicine in St. Louis. (wustl.edu)
  • Vaccinating mice against the protein reduces the ability of bacteria to cause severe disease. (wustl.edu)
  • They focused on a protein known as FmlH, which they believed was located at the tip of the pilus, where it would help bacteria stick to the bladder wall. (wustl.edu)
  • The researchers removed the gene for FmlH from E. coli and then infected the urinary tracts of mice with bacteria with and without the gene. (wustl.edu)
  • They found that E. coli lacking FmlH were less likely to establish chronic infections in mice than bacteria with the protein. (wustl.edu)
  • But by the end of the fourth week, the bacteria without FmlH were 1,000 times less numerous in the bladder and 100 times less numerous in the kidneys than the bacteria with the protein. (wustl.edu)
  • Then, they infected both groups with E. coli and measured the amount of bacteria in their bladders and kidneys one, two and three days after infection. (wustl.edu)
  • In an ode to historical accounts that claim Hippocrates used vinegar roughly 2,000 years ago to clean wounds, modern studies now show that vinegar can help kill pathogens and can also be used as a food preservative due to its ability to inhibit dangerous bacteria like E.Coli. (thepaleodiet.com)
  • University of Michigan scientists have discovered a molecular assistant they've dubbed 'Spy' that helps bacteria excel at producing proteins for medical and industrial purposes. (newenergyandfuel.com)
  • While the prime motivation is to work at proteins for medicine and industry, finding responses that improve production of desired traits is news - and it will provoke a search and might enable an improvement in bacteria working for fuel production. (newenergyandfuel.com)
  • Bacteria are dynamic creatures that are widely used to manufacture proteins used in medicine and industry as well as in research for fuel production. (newenergyandfuel.com)
  • Examples include many proteins can fall apart and get cut up inside the bacteria before they can be harvested. (newenergyandfuel.com)
  • James Bardwell, a professor of molecular, cellular and developmental biology and of biological chemistry, as well as a Howard Hughes Medical Institute investigator, at the University of Michigan is leading a research team that's developed a way to coerce bacteria into making large quantities of stable, functional proteins. (newenergyandfuel.com)
  • Through a 'directed evolution' experiment, in which the scientists selected colonies with increasing antibiotic resistance - and increasing protein stability - the team generated designer bacteria that produced up to 700 times more of the previously unstable protein. (newenergyandfuel.com)
  • To see why the designer bacteria were so much better at producing proteins, the scientists examined and found that the efficient microbes were making much more of the small protein called Spy. (newenergyandfuel.com)
  • Bardwell looks ahead saying, "Our work may usher in an era of designer bacteria that have had their folding environment customized so that they can now efficiently fold normally unstable proteins. (newenergyandfuel.com)
  • Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death ProGram. (jonbarron.org)
  • resistance may be caused by production of carbapenemases ( 2 ) or by combined mechanisms of an outer membrane permeability defect and extended-spectrum β-lactamases or cephalosporinase ( 3 - 6 ). (cdc.gov)
  • Using information from Escherichia coli derived asparagine synthetase, some basic mechanisms of the enzyme have been understood. (wikipedia.org)
  • Several recent studies have indicated that plasmid-mediated resistance mechanisms also play a significant role in fluoroquinolone resistance, and its prevalence is increasing worldwide. (biomedcentral.com)
  • The bacterial immunity proteins Im7 and Im9 fold with mechanisms of different kinetic complexity. (shengsci.com)
  • As such, they offer only short-term solutions as they usually can't overcome multiple existing resistance mechanisms and do not control the growing number of pan-resistant pathogens. (jonbarron.org)
  • The antibiotic treatment of necrotizing fasciitis from Aeromonas infections can be difficult, as antibiotic resistance mechanisms may be involved. (medscape.com)
  • However, the cost of resistance can be mitigated by compensatory mutations elsewhere in the genome, and therefore the loss of resistance may proceed too slowly to be of practical importance. (elifesciences.org)
  • The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. (utmb.edu)
  • The rhtA gene known as the ybiF ORF in the genome of Escherichia coli was identified as a new gene involved in threonine and homoserine efflux. (rhea-db.org)
  • Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. (lu.se)
  • Spectinomycin and tetracycline prevent proteins from being assembled by the cell and streptomycin induces the assembly of the wrong amino acids into the translated protein. (icr.org)
  • The gyrA and gyrB genes code for proteins of 845 and 653 amino acids, respectively. (omicsdi.org)
  • Amino acid sequencing of some beta-lactamases has shown that substitution of only a few amino acids in the bla gene leads to high-level resistance against specific cephalosporins. (lu.se)
  • This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. (nih.gov)
  • We focus our studies on the chemotaxis systems within Pseudomonas aeruginosa , and seek to elucidate how protein localization is correlated to function. (uwm.edu)
  • Through a bacterial two-hybrid library screen, we identified the E. coli β-lactam resistance protein Blr, a short membrane polypeptide of 41 residues, as an interacting partner of the essential cell division protein FtsL. (pasteur.fr)
  • Such a peculiar arrangement of phenylalanyl residues at the distal ligand binding site has not been observed before in the globin family, and is unique to HbI, a protein functionally devoted to sulfide transport. (shengsci.com)
  • A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). (dtu.dk)
  • Antibiotic resistance typically induces a fitness cost that shapes the fate of antibiotic-resistant bacterial populations. (elifesciences.org)
  • qnrA is the plasmid-mediated quinolone resistance gene encoding a 218 amino acid protein of the pentapeptide family that protects DNA gyrase from quinolone inhibition[ 4 ]. (biomedcentral.com)
  • Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B. (ubc.ca)
  • Researchers have identified a potential way to prevent chronic urinary tract infections, a common infection primarily caused by E. coli (shown above). (wustl.edu)
  • As a pathogen, E. coli are the most frequent causes of bacterial infections, including urinary tract infections, diarrheal disease, and other clinical infections such as neonatal meningitis, pneumonia and bacteremia. (eurekaselect.com)
  • In Latin America, as well as many other developing countries, diarrheal infections caused by E. coli remain an important cause de infant morbidity - mortality. (eurekaselect.com)
  • Mutagenesis in the alpha3alpha4 GyrA helix and in the Toprim domain of GyrB refines the contribution of Mycobacterium tuberculosis DNA gyrase to intrinsic resistance to quinolones. (omicsdi.org)
  • 1] In 1980 it was estimated that 3-5% of S. pneumoniae were penicillin-resistant and by 1998, 34% of the S. pneumoniae sampled were resistant to penicillin.1 Antibiotic resistance by other organisms reflects the same trend observed between S. pneumoniae and penicillin. (icr.org)
  • La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
  • In addition, we are also investigating the mechanism of partitioning chemotaxis proteins that regulate flagellar based motility. (uwm.edu)
  • protein_coding" "AAC74156","flgA","Escherichia coli","assembly protein for flagellar basal-body periplasmic P ring [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74157","flgB","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74158","flgC","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
  • Flagella basal body rod protein, Flagellar basal body rod FlgEFG protein C-terminal [Interproscan]. (ntu.edu.sg)
  • FlgD Ig-like domain, FlgD Tudor-like domain, Flagellar hook capping protein - N-terminal region [Interproscan]. (ntu.edu.sg)
  • protein_coding" "AAC74163","flgH","Escherichia coli","flagellar protein of basal-body outer-membrane L ring [Ensembl]. (ntu.edu.sg)
  • Flagellar L-ring protein [Interproscan]. (ntu.edu.sg)
  • Such studies are needed to understand the increasingly recognized diversity of enterotoxigenic E. coli, a leading cause of pediatric and travelers' diarrhea. (eurekaselect.com)
  • By targeting a non-essential virulence factor (pyomelanin), it is unlikely that resistance to the anti‑virulence agent will occur. (uwm.edu)
  • Most of them are genes that encode hypothetical transmembrane proteins. (rhea-db.org)
  • The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. (lu.se)
  • The E. coli CO strain was resistant to extended-spectrum cephalosporins, cefoxitin, and moxalactam. (cdc.gov)
  • MazG is a homodimeric alpha-helical protein that belongs to the superfamily of all-alpha NTP pyrophosphatases. (shengsci.com)
  • Search proteins in UniProtKB for this molecule. (rhea-db.org)
  • It confirmed that the enzyme belonged to a class A beta-lactamase which had 99% identity to the ampicillin resistance transposon Tn3 of pBR322. (lu.se)
  • These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
  • OXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases in Escherichia coli sequence type 131 (ST131). (ox.ac.uk)
  • Effects of Outer Membrane Protein TolC on the Transport of Escherichia coli within Saturated Quartz Sands. (uwm.edu)
  • TolC protein may play a vital role in the secretion of enterobactin. (umsystem.edu)
  • The TolC gene was cloned into a pBAD directional TOPO vector containing an N-terminal His-tag and a gene for kanamycin resistance. (umsystem.edu)
  • These data establish that TolC may be a critical component of the E. coli enterobactin secretion machinery and may represent a type of siderophore export mechanism previously undescribed. (umsystem.edu)
  • Spread of CTX-M type ESBLs, especially in Escherichia coli, may provide a favorable background for selection of carbapenem resistance. (cdc.gov)
  • 4] Failure of DNA to properly separate during these processes results in a bacterium not being able to divide normally or produce functional proteins. (icr.org)
  • Depending on the type of vinegar and what was used in the fermentation process, this liquid can also be rich in enzymes, proteins, and probiotics that are claimed to help with gut health, among other benefits. (thepaleodiet.com)
  • The replacement of M74 in GyrA, A83 in GyrA, and R447 in GyrB of Mycobacterium tuberculosis gyrase by their Escherichia coli homologs resulted in active enzymes as quinolone susceptible as the E. coli gyrase. (omicsdi.org)
  • However, in other cases, such deleterious side effects of resistance mutations are undetectable, and resistance can even confer benefits in specific, antibiotic-free environmental settings ( Maharjan and Ferenci, 2017 ). (elifesciences.org)
  • The first focuses on spatial organization of proteins within a bacterial cell. (uwm.edu)
  • Penicillins inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
  • If compensatory mutations are indeed widespread, pathogens can reach both high level of resistance and high fitness. (elifesciences.org)
  • Antibiotic resistance acquired by food borne pathogens such as E. coli has become a global concern. (techconnect.org)
  • An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. (omicsdi.org)
  • Here, we report an efficient gene editing strategy based on direct delivery of Cas9 and gRNA into Uropathogenic E. coli (UPEC) isolate. (microbiologyresearch.org)
  • The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. (lu.se)
  • A pCb plasmid encoding a beta-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. (lu.se)
  • The fast sulfide association rate constant can be related to the presence of Gln(64)E7, as the heme distal residue, together with the protein structural properties in the CD-E distal region. (shengsci.com)
  • Structural analysis of the rate-limiting transition states in the folding of Im7 and Im9: similarities and differences in the folding of homologous proteins. (shengsci.com)
  • Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein. (shengsci.com)
  • Fasciculin 2 and toxin alpha proteins belong to the same structural family of three-fingered snake toxins. (shengsci.com)
  • Structural and kinetic analyses of penicillin-binding protein 4 (PBP4)-mediated antibiotic resistance in Staphylococcus aureus. (ubc.ca)
  • Ertapenem resistance may be explained by a defect in the outer membrane protein and production of extended-spectrum β-lactamase CTX-M-2. (cdc.gov)
  • La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. (pasteur.fr)
  • We predict this structure to be a membrane protein. (expasy.org)
  • Binding-protein-dependent transport system inner membrane component [Interproscan]. (ntu.edu.sg)
  • This gene encodes a highly hydrophobic membrane protein that contains 10 predicted transmembrane segments. (rhea-db.org)
  • while some of them were classified as hypothetical proteins. (utmb.edu)
  • One of the hypothetical proteins was annotated as Z2335, with orthologue in EPEC, and by bioinformatics analysis, this protein was revealed to be the universal stress protein F (UspF). (utmb.edu)
  • This demonstrates that the primary structure of gyrase determines intrinsic quinolone resistance and was supported by a three-dimensional model of N-terminal GyrA. (omicsdi.org)
  • Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations. (omicsdi.org)
  • An ertapenem-resistant Escherichia coli isolate was recovered from peritoneal fluid in a patient who had been treated with imipenem/cilastatin for 10 days. (cdc.gov)
  • Culture of the peritoneal fluid yielded an ertapenem-resistant E. coli CO strain. (cdc.gov)
  • Conjugation experiments that used an azide-resistant E. coli J53 strain as recipient strain ( 5 ), followed by selection on Mueller-Hinton agar plates containing 100 mg/L sodium azide and 100 mg/L amoxicillin or 2 mg/L of cefotaxime, yielded transconjugants. (cdc.gov)
  • Such strategies implicitly presume that resistance leads to reduced bacterial fitness in an antibiotic-free environment, and therefore these resistant populations should be rapidly outcompeted by antibiotic-sensitive variants. (elifesciences.org)
  • A brief look at an example of penicillin resistance reveals the increase in the frequency of antibiotic-resistant organisms since the time when antibiotic use became common. (icr.org)
  • Both E. coli genes give rise to a threonine-resistant phenotype when overexpressed, and they decrease the accumulation of radioactive metabolites derived from [(14)C] L-threonine. (rhea-db.org)
  • We analyzed the resistant mutants and showed that Ser-82 of GyrA, equivalent to resistance hot spot Ser-83 of GyrA in E. coli, was in each case replaced with Phe. (omicsdi.org)
  • Transferability was examined by conjugation with the sodium azide-resistant E. coli J53. (biomedcentral.com)