• We describe the first example of folate conjugated to a protein via carbohydrate residues, using a novel SH-folate intermediate. (openrepository.com)
  • The enzyme assumes a fold related to a (beta/alpha)8 barrel, with a long groove on the surface of the protein that harbours all conserved residues. (rcsb.org)
  • N -linked glycosylation occurs when glycans are attached to asparagine residues on the protein. (neb.com)
  • N-linked glycosylation occurs through the asparagine residues of the protein, while O-linked glycosylation occurs through serine or threonine. (neb.com)
  • For structural analysis of asparagine-linked carbohydrates, sugars are released from the protein backbone by enzymes such as PNGase F ( NEB #P0704 ). (neb.com)
  • In addition, they modeled all potential glycans (or carbohydrates) attached to the S protein. (medicalxpress.com)
  • Carbohydrate, fat, or protein malabsorption is caused by a disorder in the intestinal processes of digestion, transport, or both of these nutrients across the intestinal mucosa into the systemic circulation. (medscape.com)
  • P contained 66.1% carbohydrate, 35.3% protein and 2.1% uronic acid and ranged in molecular weight from 850 to 20,000 Da. (scirp.org)
  • S contained approximately 10% carbohydrate and 15% protein and was mostly soluble in ethanol. (scirp.org)
  • Furthermore, gel column chromatography on Sephadex G-150 indicated that the elution curves of the carbohydrate and protein overlapped significantly in sample P ( Figure 1 ). (scirp.org)
  • Then, similar charges were obtained for five additional proteins (the carbohydrate-recognition domain of Galectin-3 (two different crystal structures and five different ligands), factor Xa, hisactophilin (three different protonation states of all 21 histidines), haloalkane dehalogenase, and the GCN4 leucine zipper) [S.Genheden, P. Söderhjelm, U. Ryde (2010) "Transferability of conformational dependent charges from protein simulations" J. Chem. (lu.se)
  • The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. (nih.gov)
  • Finally, these new peptides reveal a carbohydrate moiety, indicating a glycosylation in the sequence. (springer.com)
  • Peptides were characterized by a combined use of MALDI- TOF/TOF and nanoESI-IT-ETD experiments to determine not only the peptide sequence but also the composition and the position of the carbohydrate moiety. (springer.com)
  • γ-secretase processing produces several Aβ peptides with heterogeneous C termini ranging from 38 to 43 residues in length. (jneurosci.org)
  • Subsequently, brush border-bound peptidases further hydrolyze peptides to release a mixture of free amino acids and small peptides (2-3 amino acid residues). (medscape.com)
  • Other commonly used endoglycosidases such as Endoglycosidase H ( NEB #P0702 ) are not suitable for general deglycosylation of N -linked sugars, because it only deglycosylates glycoproteins containing primarily high mannose N -linked structures, as well as leaving one N -acetylglucosamine residue attached to the asparagine. (neb.com)
  • Waste husks have been explored as a source of carbohydrates and sugars, but they also contain lignin, a tough lipid polymer found in many woody plants. (scienceblog.com)
  • Considering that the main potential feedstock (biomass) for producing bioethanol is composed of carbohydrates, which include starch, cellulose and hemicelluloses, among others, the use of enzymes to break down these oligosaccharides into easily fermentable sugars is a requirement, previous to conducting the fermentation ( Kim and Dale, 2004 ). (scialert.net)
  • High-carbohydrate diets undermine dental health because these foods' residue in the mouth break down into sugars, which feed the Streptococcus mutans bacteria which are also present. (eurasiareview.com)
  • 1975. The effect of atmospheric levels of pesticides on pesticide residues in rabbit adipose tissue and blood sera. (cdc.gov)
  • One strategy to help contain the increased costs of organic foods is to consider the Environmental Working Group's (EWG) annual lists of pesticide levels that list the dirty dozen (produce that is contaminated with more pesticides than other crops) and the clean fifteen (produce that has the lowest amounts of pesticide residues). (msdmanuals.com)
  • When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates. (neb.com)
  • Agricultural residues can thus be used for the production of various value-added products, such as industrially important enzymes. (mdpi.com)
  • The PdaA structure provides a template for interpreting the wealth of sequence data on family 4 carbohydrate esterases in a structural context and represents a first step towards understanding the reaction mechanism of this family of enzymes. (rcsb.org)
  • The enzymes contain ~250-300 residues, which encode putative signal sequences and carbohydrate attachment sites. (embl.de)
  • Family 4 carbohydrate esterases deacetylate polymeric carbohydrate substrates such as chitin, acetyl xylan and peptidoglycan. (rcsb.org)
  • We've consolidated the application notes you need on a range of topics, including corn syrup, dietary fiber, lactose and lactulose, maltodextrins, sialic acids and simple carbohydrates. (thermofisher.com)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • In previous structures of P2Ox, the substrate loop (residues 452-457) covering the active site has been either disordered or in a conformation incompatible with carbohydrate binding. (nih.gov)
  • The digestibility of dietary carbohydrates depends, among others, on structural properties of the carbohydrate, such as the degree of polymerization (DP) and branching. (frontiersin.org)
  • Change to a high digestibility/low residue diet. (vin.com)
  • In the present study, the utilization of some agricultural residues (like mango residues) containing carbohydrates for the production of bioethanol was evaluated. (scialert.net)
  • Chemical glycosylation Fischer glycosidation Glycosyl halide Koenigs-Knorr reaction Carbohydrate acetalisation Trimethylsilyl Benzyl Ether para-methoxybenzyl ether Carbohydrates are reactants in many organic reactions. (wikipedia.org)
  • Carbohydrate chemistry is a subdiscipline of chemistry primarily concerned with the detection, synthesis, structure, and function of carbohydrates. (wikipedia.org)
  • Carbohydrate synthesis is a sub-field of organic chemistry concerned specifically with the generation of natural and unnatural carbohydrate structures. (wikipedia.org)
  • Posters and talks presented at this GRS will focus on the study of carbohydrate chemistry and biology, looking at areas such as synthesis as well as glycans' behavior both in vivo and in silico , among many more. (grc.org)
  • Prebiotics are carbohydrates that are selectively fermented by gut bacteria which provide health benefits to people who consume them. (usda.gov)
  • The tentative determinant for discriminating between the two binding modes is the position of the O6 hydroxyl group, which in the C2-oxidation mode can make favorable interactions with Asp452 in the substrate loop and, possibly, a nearby arginine residue (Arg472). (nih.gov)
  • This can include the synthesis of monosaccharide residues or structures containing more than one monosaccharide, known as oligosaccharides. (wikipedia.org)
  • However, chlordane residue is still present from prior use in many homes and other structures, as well as the surrounding soil. (cdc.gov)
  • The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. (nih.gov)
  • Conjugation of folate via gelonin carbohydrate residues retains ribosomal-inactivating properties of the toxin and permits targeting to folate receptor positive cells. (openrepository.com)
  • It is now well recognized that the bacteria living in our gut play an important role in the development and maintenance of the innate and adaptive immune system ( 1 , 2 ) as well as the fermentation of low-, or non-digestible dietary carbohydrates ( 3 - 5 ). (frontiersin.org)
  • They serve as a carbohydrate source for the fermentation process. (burg-halle.de)
  • Fribous (lignocellulosic) is the second major source of carbohydrates in hard woods and vegetable biomass, so its fermentation is essential for the economic conversion of starch to ethanol, which may provide an ideal alternative fuel source in the future ( Lin and Tanaka, 2006 ). (scialert.net)
  • Low-residue diets are available through your veterinarian. (vin.com)
  • Prominent organizations including the World Health Organization and U.S. Department of Agriculture (USDA) have dismissed low-carbohydrate diets that prevent tooth decay in favor of recommending high-carbohydrate diets which rely on fluoride and food fortification to mitigate dental damage and nutritional shortcomings, a University of Washington researcher says. (eurasiareview.com)
  • In a recent article published in MDPI's Nutrients journal, Dr. Philippe Hujoel of the UW School of Dentistry says that not only these organizations but other major professional and health associations reversed earlier positions and began recommending high-carbohydrate diets over the course of decades in the last century. (eurasiareview.com)
  • In the meantime, Dr. Hujoel says, some scientists provided compelling evidence that low-carbohydrate diets were at least as beneficial to health as high-carbohydrate diets. (eurasiareview.com)
  • Low-carbohydrate diets help prevent tooth decay and make fluoride - which has no tangible health benefits other than preventing cavities - largely unnecessary, he says. (eurasiareview.com)
  • Dietary supplementation with complex carbohydrates is known to alter the composition of gut microbiota, and optimal implementation of the use of these so called "prebiotics" could be of great potential in prevention and possibly treatment of obesity and associated cardiometabolic and inflammatory diseases via changes in the gut microbiota. (frontiersin.org)
  • An alternative to this "microbiocentric view" is the idea that health-promoting effects of certain complex carbohydrates reside in the host, and could secondarily affect the diversity and abundance of gut microbiota. (frontiersin.org)
  • To circumvent this potential interpretational problem, we aimed at providing an overview about whether and how dietary supplementation of different complex carbohydrates changes the gut microbiome in healthy non-obese individuals. (frontiersin.org)
  • We then reviewed whether the reported changes in gut bacterial members found to be established by complex carbohydrates would benefit or harm the cardiometabolic and immunological health of the host taking into account the alterations in the microbiome composition and abundance known to be associated with obesity and its associated disorders. (frontiersin.org)
  • By combining these research areas, we aimed to give a better insight into the potential of (foods containing) complex carbohydrates in the treatment and prevention of above-mentioned diseases. (frontiersin.org)
  • We conclude that supplemental complex carbohydrates that increase Bifidobacteria and Lactobacilli, without increasing the deleterious Bacteroides , are most likely promoting cardiometabolic and immunological health in obese subjects. (frontiersin.org)
  • Because certain complex carbohydrates also affect the host's immunity directly, it is likely that host-microbiome interactions in determination of health and disease characteristics are indeed bidirectional. (frontiersin.org)
  • For diet-driven alterations of gut microbial composition particularly the type and proportion of complex indigestible carbohydrates that reach the colon turn out to be of great importance ( 18 , 19 , 21 - 24 ). (frontiersin.org)
  • The first meeting of the Gordon Research Seminar (GRS) on complex carbohydrates is a unique opportunity for young scientists (graduate students, post-docs, and early faculty) to present and exchange cutting-edge research and ideas in an environment mainly consisting of their direct peers. (grc.org)
  • This biologically active ingredient, found mostly in Asia and Africa, is a complex carbohydrate that consists of mainly galactose residues. (lorealparisusa.com)
  • Fiber Some foods contain fiber, which is a tough complex carbohydrate. (msdmanuals.com)
  • The determination of the types and concentrations of carbohydrates in foods is integral for energy evaluation, nutritional labeling, quality control, and for identifying possible adulteration. (thermofisher.com)
  • Gluten is the rubbery residue remaining once corn has had most of its starchy carbohydrate washed out of it. (dogfoodadvisor.com)
  • C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. (lu.se)
  • Dr. Hujoel's study traces this development toward fluoride-supplemented high-carbohydrate dietary guidelines back to the mid-twentieth century, when leaders like Emory W. Morris, a dentist and president of the Kellogg Foundation - an arm of a major cereal maker - became the first chairman of the ADA's Council on Dental Health in 1942. (eurasiareview.com)
  • The enormous volume of fruit and vegetable processing residues, such as orange peels and sugar beet pulp, represents an underutilized domestic resource of valuable health-promoting compounds. (usda.gov)
  • The third difference is the very high proportion of proline residues in the sequence accounting for about one-third of the sequence. (springer.com)
  • Glycobiology is the study of the structure, function and biology of carbohydrates, also called glycans, which are widely distributed in nature. (neb.com)
  • O -linked glycans are most commonly attached to serine or threonine residues through the N -Acetylgalactosamine residue. (neb.com)
  • Dr. Hujoel's study also explores the private interests involved when the ADA took the first significant steps toward endorsing the current fluoride-supplemented high-carbohydrate nutritional guidelines, most of which have been maintained for decades. (eurasiareview.com)
  • Removes organic debris and residues, biofilm, inorganic soils, tissue, mucous and blood from instruments and general medical equipment. (lrultrasonics.com)
  • Alcohol is not effective in removing sugar residue from the skin. (medlineplus.gov)
  • Changing to a low residue diet means that most of the nutrients of the food are digested and absorbed by the pet before they reach the colon where the gas-forming bacteria are. (vin.com)
  • They are the primary pathogens associated with residue in an linkage (same binding site used for recog- shellfishborne outbreaks worldwide ( 3,4 ). (cdc.gov)
  • We demonstrated, for the first time, that carbohydrates derived from pectin found in orange peel have prebiotic properties. (usda.gov)
  • 160 residues with broad specificity glycan-binding properties, located at the N-terminus of GH33 sialidases. (cazy.org)
  • Carbohydrates in Alcoholic Beverages and Health. (chipsbooks.com)
  • Overall, this review article shows that whereas it is relatively clear in which direction supplemental fermentable carbohydrates can alter the gut microbiome, the relevance of these changes regarding health remains controversial. (frontiersin.org)
  • These groups, he says, ignored scientific evidence weakening their claim that the only adverse health effect to a high-carbohydrate diet was dental cavities. (eurasiareview.com)
  • 200 residues, found at the N-terminus of GH33 sialidases. (cazy.org)
  • They next confirmed the isolated lignin's quality and high purity, finding no evidence of carbohydrates or other contaminants. (scienceblog.com)
  • Ideally, rice would be the diet's carbohydrate source. (vin.com)
  • 1W1B: Structure of Bacillus subtilis PdaA with Cadmium, a family 4 Carbohydrate esterase. (rcsb.org)
  • Also, In Burkina Faso, enormous quantities of mango are lost per year and contribute to increase the environmental rate residues. (scialert.net)
  • He had a conflict of interest in this decision, as cereals are carbohydrates and do increase tooth decay risk. (eurasiareview.com)
  • In addition, PLA can be produced from algae and organic residues such as old bread or olive pits. (burg-halle.de)
  • 1986. A follow-up study of maternal milk contamination with organochlorine insecticide residues. (cdc.gov)
  • Sometimes just going through a case and/or bag of a low residue diet solves the problem and the pet can return to a regular food afterwards. (vin.com)
  • Carbohydrates are important food components affecting taste and nutrition. (thermofisher.com)
  • 1987. Central nervous system toxicity of some common environmental residues in the mouse. (cdc.gov)
  • The N-terminal contains four conserved CYSTEINE residues and may be modified depending on function. (bvsalud.org)