• oocyte
  • To achieve this goal, clinical scientist linkurl:Doug Turnbull;http://www.cnmd.ac.uk/researchgroups/dougturnbull and his colleagues at Newcastle University in the UK transplanted the nuclei from one human oocyte (termed pronuclei) into an oocyte whose pronuclei had been removed. (the-scientist.com)
  • The resulting embryos, which contained predominately mtDNA from the recipient oocyte, were then raised in culture for about a week. (the-scientist.com)
  • The technique consists of taking an enucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
  • work
  • In her work with chick embryos, Margaret Lewis studied connective tissue formation within the tissues as well as outside of an environment where factors involved in coagulation are present. (wikipedia.org)
  • Based on the publication of this work, Morgan was awarded his Ph.D. from Johns Hopkins in 1890, and was also awarded the Bruce Fellowship in Research. (wikipedia.org)
  • The groups initially collaborated in researching diabetes before Eggan's group switched to work on neurodegenerative diseases. (wikipedia.org)
  • Eggan's work as of 2007[update] has succeeded in developing a technique of merging stem and skin cells that has obtained considerable public attention as a possible avenue to avoid moral objections regarding stem cell research in the context of serious illness. (wikipedia.org)
  • Eggan himself is cautious about his team's work, with an early stage 2005 profile in Nature noting there was still much work to do: The hybrids still contain two nuclei: one from a skin cell and one from an embryonic stem cell. (wikipedia.org)
  • medical
  • Nonetheless, the results are "very promising," said neurogeneticist linkurl:Carolyn Sue;http://www.kolling.usyd.edu.au/research/neurogenetics-group/csue.php of the Kolling Institute of Medical Research at the University of Sydney in Australia, who was also not directly involved in the research. (the-scientist.com)
  • Eggan took on a second role as the assistant investigator for Stowers Institute for Medical Research, a philanthropical medical research group in Kansas City, Missouri. (wikipedia.org)
  • cell
  • Attention also goes to the attitudes held on the creation of hybrid embryos for stem cell research. (innovations-report.com)
  • It is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)
  • After being inserted into the egg, the somatic cell nucleus is reprogrammed by its host egg cell. (wikipedia.org)
  • Margaret Adaline Reed Lewis (1881-1970) was an American cell biologist and embryologist who made contributions to cancer research and cell culture techniques, and was likely the first person to successfully grow mammalian tissue in vitro. (wikipedia.org)
  • In 1908, Margaret Reed researched in Berlin in Max Hartmann's lab where she performed probably the first in vitro mammalian cell culture with guinea pig bone marrow by explanting the bone marrow and placing it into a nutrient-rich agar produced by fellow lab researcher Rhoda Erdmann and incubating the sample. (wikipedia.org)
  • Since the Lewises main interest was microscopic cell structures, their objective was to create optically clear media, which led to the creation of the Locke-Lewis solution. (wikipedia.org)
  • As a result, this couple's greatest impact on embryology and cell biology in the twentieth century was teaching later generations of biologists the basic factors involved in tissue culture based on what they had learned from their research. (wikipedia.org)
  • With so many avenues opened by cell culture to explore, Margaret Lewis and her husband diverged in their area of study, with Margaret Lewis choosing to focus on microbiological problems, which involved close observations of chick embryo intestines reacting to typhoid bacilli in the medium in which it was grown. (wikipedia.org)
  • In 2011, scientists at the New York Stem Cell Foundation announced that they had succeeded in generating embryonic stem cell lines, but their process involved leaving the oocyte's nucleus in place, resulting in triploid cells, which would not be useful for cloning. (wikipedia.org)
  • Eggan began to explore both this process and also the reasons that cloned animals often appeared to develop abnormally, with organ defects and immunological problems - his first contact with stem cell research. (wikipedia.org)
  • Federal funding for stem cell research had recently been removed, and part of his role was to obtain private funding to replace it. (wikipedia.org)
  • study
  • The oocytes used in the study were abnormally fertilized embryos generated during in vitro fertilization (IVF) treatments that would have otherwise been discarded, but, to be approved for clinical use, the technique would have be tested in healthy embryos, researchers say. (the-scientist.com)
  • There he worked with German biologist Hans Driesch, whose research in the experimental study of development piqued Morgan's interest. (wikipedia.org)
  • technique
  • They suggest the technique could ultimately prevent the transmission of mitochondrial diseases if doctors moved nuclei from a fertilized egg carrying the disease to another egg with disease-free mitochondria. (the-scientist.com)
  • Refining their technique, the researchers were able to limit the amount of mitochondrial transfer, creating manipulated embryos with less than 2 percent donor mitochondria, "which we felt was a significant breakthrough in this area," Turnbull said. (the-scientist.com)