ProteinsGeneNucleasesKRABTranscription factorC2H2ResiduesHomeoboxGenomeCysteineInhibition20212017DestabilizesNuclease1995McGovern InstitutNuclear2018Sigma-Aldrich19981988AnalysesMutationSequencesDomainEvolutionaryInhibitsHumanCancer ResearchCopperVirusesStructuresInteractionsPathologyInfectionClinicalCellEmbryosScientific researchWorkSCOPeBiophysicsRegionBiologicalMolecularExpressionExplore
Proteins10
- Zfwd: a Novel Subfamily of Plant Proteins Containing a C3h Zinc Finger" by J. Terol, M. Bargues et al. (usu.edu)
- We describe a new subfamily of WD repeat proteins characterised by the presence of a C3H zinc finger at the N-terminal part of the protein associated with seven WD40 repeats. (usu.edu)
- and Perez Alonso, M., "Zfwd: a Novel Subfamily of Plant Proteins Containing a C3h Zinc Finger and Seven Wd40 Repeats" (2000). (usu.edu)
- Fusion proteins containing all three zinc fingers were found in the nucleus, but those containing only two zinc fingers were predominantly cytoplasmic. (wustl.edu)
- Engineering RNA-Binding Proteins by Modular Assembly of RanBP2-Type Zinc Fingers. (rochester.edu)
- Several mammalian proteins involved in chromatin and DNA modification contain CXXC zinc finger domains. (cipsm.de)
- Choose from an industry leading selection of Cas9 proteins to meet your individual research needs. (sigmaaldrich.com)
- Our current projects involve the expression and purification of zinc-finger proteins in the hopes of understanding their structures. (hood.edu)
- Prior to joining FDA, she conducted postdoctoral research at the National Institutes of Health studying protein-protein and protein-DNA interactions involving zinc finger proteins. (fda.gov)
- Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
Gene13
- This gene encodes a MYND-containing zinc-binding protein with a nuclear localization sequence. (nih.gov)
- Taking into account specific alpha-helical propensities of Val and Met, this mutation is likely to destabilise the alpha helix of the zinc finger that forms the contact with the DNA duplex, thus affecting the biological function as shown by reporter-gene assays. (bmj.com)
- NGFI-A is an immediately-early gene that encodes a transcription factor whose DNA binding domain is composed of three C 2 H 2 zinc fingers. (wustl.edu)
- abstract = "NGFI-A is an immediately-early gene that encodes a transcription factor whose DNA binding domain is composed of three C2H2 zinc fingers. (wustl.edu)
- so Sangamo have applied zinc fingers to fairly obvious targets like trying to switch off the gene VEGFA. (webofstories.com)
- As a result of this evolutionary armsrace , both retrotransposons and KRAB zinc finger genes become heavily integrated in pre-existing gene regulatory networks, adding an extra level of complexity to how, where and when genes in our genome are shut on or off. (uva.nl)
- Our research aims to pinpoint how specific classes of transposable elements have contributed to the evolution of human neuronal gene expression networks and understand how these changes may relate to human's increased susceptibility to neurodevelopmental disorders such as Autism and Schizophrenia and human neurodegenerative diseases such as Alzheimer's and Parkinson's disease. (uva.nl)
- Current research in my group focusses on neurally expressed multi-copy genes and the impact of gene duplication events on the evolution of human neural gene-regulatory pathways. (uva.nl)
- Sigma-Aldrich ® Advanced Genomics delivers complete support for every step of CRISPR-based gene editing research at any scale. (sigmaaldrich.com)
- Target a single gene using Zinc Finger Nucleases or screen a whole genome with fully customizable CRISPR products. (sigmaaldrich.com)
- Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench. (sigmaaldrich.com)
- Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich ® Advanced Genomics is here to guide you. (sigmaaldrich.com)
- CompoZr Zinc Finger Nucleases (ZFNs) are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections. (sigmaaldrich.com)
Nucleases2
- With the development of novel genome modification tools, such a zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas, it is now possible to efficiently engineer non-mammalian models of inherited human diseases. (nih.gov)
- Zinc-finger nucleases. (nih.gov)
KRAB2
- we showed how KRAB zinc finger genes in our genome are in a continuous battle against retrotransposon invasions, revealing how our genome is actually in a war against itself. (uva.nl)
- A study now demonstrates that a primate-specific regulatory network controlled by the KRAB zinc-finger protein ZNF808 is essential for pancreas development. (nature.com)
Transcription factor2
- Prior research implicated nine members of the zinc-finger transcription factor family in Schwann cell (SC) development and myelination, and levels of one such protein were reported increased in other non-myelinating SCs after denervation. (tamu.edu)
- WT-1, a zinc-finger transcription factor expressed in the metanephric mesenchyme, is essential for ureteric bud outgrowth. (medscape.com)
C2H22
- Furthermore, the mutation of an arginine residue in the third zinc finger of NGFI-A, a position which is occupied by a leucine residue in most C2H2 zinc fingers, abolished nuclear localization, but had no effect on DNA binding. (wustl.edu)
- Journal Article] Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β. (nii.ac.jp)
Residues2
- In the same manner as ADA, the compounds interact with an 18-residue polypeptide on the N terminal zinc knuckle region of the HIV nucleocapsid protein which causes ejection of the zinc from the region by covalently modifying the cysteine residues. (wikipedia.org)
- They react with the cysteine residues on the zinc finger of the NCp7 and cause a covalent conformation change which ejects the zinc from the zinc finger domain. (wikipedia.org)
Homeobox1
Genome3
- J:99680 The FANTOM Consortium and RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group), The Transcriptional Landscape of the Mammalian Genome. (jax.org)
- With the completion of the Human Genome Project in 2003 and the International HapMap Project in 2005, researchers now have a powerful set of research tools, including the high-speed DNA sequencing technology that make it possible to identify the genetic contributions to specific diseases, even if they are rare. (nature.com)
- The Sigma-Aldrich ® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. (sigmaaldrich.com)
Cysteine3
- ADA inhibits HIV by electrophilically attacking the sulfur atoms of the zinc coordinated cysteine. (wikipedia.org)
- When the zinc finger structure was altered by mutating a zinc-chelating cysteine residue in any one of the three zinc fingers, the resulting domain was no longer capable of directing β-galactosidase to the nucleus. (wustl.edu)
- LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. (thermofisher.com)
Inhibition4
- As zinc fingers are known to be involved in m-RNA regulation, reverse transcription, protection of synthesized viral DNA, transcription inhibition, and initial integration processes, prevention of zinc finger function can have drastic effects on the function of the cell or virus. (wikipedia.org)
- Antagonistic effects of zinc and aluminum on lead inhibition of *-aminolevulinic acid dehydratase. (cdc.gov)
- 1994. Inhibition of Na+-glucose cotransport in kidney cortical cells by cadmium and copper: Protection by zinc. (cdc.gov)
- 1976. The in vitro effect of zinc on the inhibition of human *-aminolevulinic acid dehydratase by lead. (cdc.gov)
20171
- The global food and agricultural industry for 2017 totaled about $8.1 trillion, according to Plunkett Research estimates, or about 10% of the world's GDP. (plunkettresearch.com)
Destabilizes2
- This electrophilic interaction destabilizes the zinc binding site making it easier for the zinc ion to be withdrawn due to the new arrangement of bonds. (wikipedia.org)
- Tristetraprolin/zinc finger protein 36 (TTP/ZFP36) binds and destabilizes some pro-inflammatory cytokine mRNAs. (usda.gov)
Nuclease1
- However, by using Sigma-Aldrich CompoZr ® Zinc Finger Nuclease (ZFN) technology, HepaRG transporter knockout cell lines have been created empowering more accurate research investigations of liver transporters. (prnewswire.com)
McGovern Institut1
- He is at the Broad Institute and is a professor of neuroscience at the McGovern Institute for Brain Research. (medscape.com)
Nuclear1
- Furthermore, the mutation of an arginine residue in the third zinc finger of NGFI-A, a position which is occupied by a leucine residue in most C 2 H 2 zinc fingers, abolished nuclear localization, but had no effect on DNA binding. (wustl.edu)
20181
- HOUSTON, Texas - Plunkett Research, Ltd. recently announced an important market research and competitive analysis report, Food Industry Almanac, 2018 edition . (plunkettresearch.com)
Sigma-Aldrich2
- The ability to accurately predict drug transporter interactions in the liver is a critical part of developing safer and more efficacious therapies," said Paul Brooks , Ph.D., Head of Discovery Research Services at Sigma-Aldrich. (prnewswire.com)
- Sigma- Aldrich , a leading Life Science and Technology company focused on enhancing human health and safety, manufactures and distributes 250,000 chemicals, biochemicals and other essential products to more than 1.4 million customers globally in research and applied labs as well as in industrial and commercial markets. (prnewswire.com)
19982
- 1998. Zinc acetate treatment in Wilson's disease. (cdc.gov)
- 1998. The effect of zinc supplementation on the effects of lead on the rat testis. (cdc.gov)
Analyses1
- Biochemical and activity-based probe analyses identify a non-canonical zinc finger domain as the E3 active site, which utilizes the strictly conserved Cys4462, not involved in zinc coordination, as the reactive nucleophile. (biorxiv.org)
Mutation2
- Since the CCHC binding site is mutation resistant and involved in the replication of HIV-1, it makes a prime candidate for the prevention of HIV through zinc ejectors. (wikipedia.org)
- The maternal PLZF allele harbours a recessive missense mutation (c.1849A→G), which leads to the substitution of a highly conserved methionine by valine (p.Met617Val) within a zinc-finger motif. (bmj.com)
Sequences1
Domain4
- The binding of zinc (II) in the CCHC binding site is necessary for the domain to be functional and for the stabilization of the conformation of the structure, allowing the NCp7 to carry out the processes required for HIV replication. (wikipedia.org)
- The cryo-EM structure of the trapped RNF213:E2∼Ub intermediate reveals RNF213 C-terminal domain as the E2 docking site, which positions the ubiquitin-loaded E2 proximal to the catalytic zinc finger, facilitating nucleophilic attack of Cys4462 on the E2∼Ub thioester. (biorxiv.org)
- Description: A sandwich ELISA kit for detection of Zinc Finger, AN1-Type Domain Protein 6 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (operatiebrp.nl)
- Journal Article] Crystallization and preliminary X-ray diffraction analysis of human importin β-Snail zinc finger domain complex. (nii.ac.jp)
Evolutionary1
- The planktonic microcrustacean Daphnia pulex is among the best-studied animals in ecological, toxicological and evolutionary research. (biomedcentral.com)
Inhibits1
- The binding site then performs a disulfide exchange, forming new intermolecular disulfide bonds, and rearrangement occurs placing the zinc finger in a conformation that inhibits its function. (wikipedia.org)
Human6
- Other viruses such as SARS, polio, Ebola, measles, human coxsackie, Dengue, rabies, human hepatitis, human parainfluenza and human respiratory syncytical have similar zinc finger motifs and could potentially benefit from zinc finger inhibitor technology. (wikipedia.org)
- RNF213/ALO17/mysterin (Ring Finger Protein 213) is the largest known human single-chain E3 ubiquitin ligase, first described as the major susceptibility factor for Moyamoya disease (MMD) ( 1 ), a disorder which causes aberrant vasculature development commonly leading to stroke and other complications ( 2 ). (biorxiv.org)
- Description: A competitive ELISA for quantitative measurement of Human AN1 type zinc finger protein 6(ZFAND6) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (operatiebrp.nl)
- We are a biomedical discovery institute researching the biology underlying human health. (crick.ac.uk)
- Implementing human genetics research: an itinerary of a researcher from low-income settings. (nature.com)
- This enabled them to identify a Zinc finger protein (ZNF) specifically expressed in humans and not chimpanzees and demonstrate that the human-specific ZNF (named ZNF558) is crucial for both mitochondrial homeostasis and early human brain development. (lu.se)
Cancer Research2
- I am the General Secretary of the International Society of Breast Pathology, Breast Specialty Editor of Histopathology, a member of the National Coordinating Committee of Breast Pathology (BIG18), National Institute of Cancer Research (NICR) Breast Clinical Studies Group (CSG) and its Breast Translational Subgroup, Breast Cancer Screening Programme Research Advisory Committee, the Pathological Society of Great Britain and Ireland Management Committee, and the international Breast Pathology Group. (nottingham.ac.uk)
- Breast Cancer Research. (lu.se)
Copper6
- 1990. A review on biochemical roles, toxicity and interactions of zinc, copper and iron: IV. (cdc.gov)
- Zinc and copper modulate differentially the P2X receptor. (cdc.gov)
- Radial and median nerve conduction velocities in workers exposed to lead, copper, and zinc: A follow-up study for 2 years. (cdc.gov)
- 1992. Auditory event-related potential (P300) in relation to peripheral nerve conduction in workers exposed to lead, zinc, and copper: Effects of lead on cognitive function and central nervous system. (cdc.gov)
- 1992. Use of zinc acetate to treat copper toxicosis in dogs. (cdc.gov)
- The Effect of Circulating Zinc, Selenium, Copper and Vitamin K on COVID-19 Outcomes: A Mendelian Randomization Study. (cdc.gov)
Viruses1
- He began research as a PhD student of Rosalind Franklin's at Birkbeck College, London in 1955 studying the structure of small viruses by x-ray diffraction. (webofstories.com)
Structures1
Interactions1
- Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. (thermofisher.com)
Pathology1
- I am the leading member of the multi-disciplinary Nottingham Breast Research and Digital Pathology Research Group at the University of Nottingham. (nottingham.ac.uk)
Infection3
- 3-nitrosobenzamide (NOBA) and 6-nitroso-1,2-benzopyrone (NOBP) were the first compounds to demonstrate an ability to inhibit infection of HIV by ejecting zinc from NCp7. (wikipedia.org)
- and Ke- day growth curve in which cells were infected at a mul- nya Medical Research Institute, Nairobi, Kenya (R. Sang) tiplicity of infection of 0.01 and aliquots removed daily. (cdc.gov)
- I did research with Don Wiley, who is a structural biologist, and also Xiaowei Zhuang looking at virus infection. (medscape.com)
Clinical4
- Azodicarbonamide (ADA) was the first zinc ejector to go into clinical trial for treatment of HIV. (wikipedia.org)
- This product is for research use only and is not approved for use in humans or in clinical diagnosis. (novusbio.com)
- Opportunities for clinicians to perform innovative biomedical discovery research for three years while following the Crick's clinical PhD programme. (crick.ac.uk)
- The exploration of advanced probes for cancer diagnosis and treatment is of high importance in fundamental research and clinical practice. (bvsalud.org)
Cell3
- Description: A competitive ELISA for quantitative measurement of Rat AN1 type zinc finger protein 6(ZFAND6) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (operatiebrp.nl)
- Here, we used the rat cervical sympathetic trunk (CST), an autonomic nerve in which non-myelinating SCs are the predominant cell type, to isolate zinc-finger protein (ZFP) cDNAs by reverse transcriptase-polymerase chain reaction. (tamu.edu)
- The Zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim. (ozgene.com)
Embryos1
- An important bottleneck in fish aquaculture research is the supply and maintenance of embryos, larvae, juvenile and adult specimens. (researchgate.net)
Scientific research2
- Our mission is to pursue scientific research without boundaries. (crick.ac.uk)
- Open Access is an initiative that aims to make scientific research freely available to all. (intechopen.com)
Work3
- This work was supported by institutional research grants. (bmj.com)
- If you would like to give to a specific research program or support the work of a particular researcher, contact our fundraising team today. (edu.au)
- The genetic aspect of renal malformation has not been fully understood yet, and there is much current research work in this field. (medscape.com)
SCOPe1
Biophysics1
- He then moved to Heidelberg to open the Department of Biophysics at the Max Planck Institute for Medical Research where he remained as director until his retirement. (webofstories.com)
Region2
Biological2
- Frances Namuswe is a Lead Chemist in the Office of Biotechnology Products (OBP), which conducts review, regulation, and research of biological products and biosimilar products. (fda.gov)
- We hope that this review could inspire more research interests in the development of tumor-targeting theranostic probes for advanced biological studies. (bvsalud.org)
Molecular1
- John Finch is a retired member of staff of the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK. (webofstories.com)
Expression1
- Induction of zinc-finger proliferation 1 expression in non-myelinating Schwann cells after denervation. (tamu.edu)
Explore2
- Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. (curehunter.com)
- Explore a selection of reports that spotlight particular areas of research at the Crick over the past five years. (crick.ac.uk)