• Expression
  • Evaluation of target protein expression level 16. (1004682.gq)
  • for tetracycline-inducible bacteriophage-T7-based expression in the LEXSY host T7.Two mutations in the tetracycline repressor. (1004682.gq)
  • withthepcDNA6/tetracycline repressor expression vector. (1004682.gq)
  • Protein Expression & Production LEXSY protein. (1004682.gq)
  • Knockdown of SAP155 or FIR was used to investigate their reciprocal influence on each other and on c-myc transcription, pre-mRNA splicing, and protein expression. (aacrjournals.org)
  • Bunker, C. A. and Kingston, R. E. (1995) Identification of a cDNA for SSRP1, an HMG-box protein, by interaction with the c-Myc oncoprotein in a novel bacterial expression screen. (springer.com)
  • More specifically, TetR represses the expression of TetA, a membrane protein that pumps out substances toxic to the bacteria, by binding the TetA operator. (wikipedia.org)
  • Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation. (wikipedia.org)
  • Amino Acids
  • Δ324 found at low levels in human and mouse cells - an alternative splice variant encoding an 88kDa protein lacking zinc fingers 6 and 7 Δ105 variant is unique to mice, and results in a protein truncated by 105 amino acids at the acidic C-terminus. (wikipedia.org)
  • tetR
  • Tet Repressor proteins (otherwise known as TetR) are proteins playing an important role in conferring antibiotic resistance to large categories of bacterial species. (wikipedia.org)
  • TetR is one of many proteins in the TetR protein family, which is so named because TetR is the most well characterized member. (wikipedia.org)
  • These domains mainly consist of a helix-turn-helix (HTH) motif that is common in TetR protein family members (see below). (wikipedia.org)
  • Homeodomain
  • The Proline-Rich Homeodomain protein (PRH/Hex) is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. (bham.ac.uk)
  • interaction
  • Cooperativity between KorB and either KorA or TrbA repressor proteins is a key element of these circuits and deletion analysis has implicated the conserved C-terminal domain of KorAand TrbAin this interaction. (bham.ac.uk)
  • Studies on mutants showed that tyrosine 84 (or phenylalanine, in some alleles) is dispensable for repressor activity but critical for the specific interaction with KorB in both in vivo reporter gene assays and in vitro electrophoretic mobility shift and co-purification assays. (bham.ac.uk)
  • This study aims to study the significance of the FIR-SAP155 interaction for the coordination of c-myc transcription, pre-mRNA splicing, and c-Myc protein modification, as well as to interrogate FIRΔexon2 for other functions relating to altered FIR pre-mRNA splicing. (aacrjournals.org)
  • isoforms
  • Two transcript variants encoding different isoforms have been found for this gene, and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi apparatus. (wikipedia.org)
  • A large number of transcript variations exist, encoding different isoforms or chimeric proteins. (wikipedia.org)
  • 1992
  • Within each A/P domain, a different HOX protein assigns positional identities which are eventually realized in diverse morphological structures on the segmented A/P axis of the Drosophila embryo ( McGinnis and Krumlauf, 1992 ). (embopress.org)
  • mRNA
  • Ribosome moves in the 5' ⇒ 3' direction along the mRNA, making the protein from its amino (N) to carboxyl (C) terminus. (brainscape.com)
  • This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of cap-dependent mRNA translation. (wikipedia.org)
  • A gene is transcribed (copied) from DNA into RNA, which can either be non-coding (ncRNA) with a direct function, or an intermediate messenger (mRNA) that is then translated into protein. (wikipedia.org)
  • Most of these relate to post-transcriptional modification of pre-mRNAs to produce mature mRNA ready for translation into protein. (wikipedia.org)
  • molecules
  • Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus.Thomas Simonson's Research Group. (1004682.gq)
  • They are usually constantly expressed so the cell always has a supply of repressor molecules on hand. (wikipedia.org)
  • kinase
  • Phosphorylation of Thr at position 148 is detected in response to various stress conditions such as UV irradiation, oxidative stress, and anisomycin treatment or JDP2 is also regulated by other kinases such as p38 MAPK and doublecortin like protein kinase. (wikipedia.org)