• In molecular biology, a primosome is a protein complex responsible for creating RNA primers on single stranded DNA during DNA replication. (wikipedia.org)
  • The influenza virus contains a protein called the nucleoprotein, which manages expression and replication of the virus's RNA-based genome. (acs.org)
  • The team found subtle differences between the protein fragments in the two vaccines - including in the influenza's "nucleoprotein", part of the virus's replication machinery. (cosmosmagazine.com)
  • In addition, a DNA construct expressing a fusion protein between M2e and influenza virus nucleoprotein (M2eNP) was evaluated to see if the broad-spectrum protection conferred by antibodies could be further enhanced by T helper cells and cytotoxic T cells. (microbiologyresearch.org)
  • The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
  • Besides their function in the virus replication cycle, the viral glycoprotein, nucleoprotein, minor matrix protein and polymerase cofactor are viral determinants of pathogenicity, with evasion of the host innate and adaptive immune responses as the main mechanism. (biomedcentral.com)
  • The RNA is encapsidated by nucleoprotein (N protein) forming the ribonucleoprotein (RNP) complex. (wikidoc.org)
  • Infection leads to injection of the virus nucleoprotein core (consisting of many gag-derived proteins, full-length genomic RNA, and the reverse transcriptase protein). (ukessays.com)
  • Once inside the cell, the nucleoprotein complex accesses intracellular DNA nucleotide triphosphate pools, where the reverse transcriptase protein initiates and creation of a double-stranded DNA copy of the genome of the virus is prepared for integration into the host cell chromosome. (ukessays.com)
  • The nucleocapsid protein has different functions in the coronavirus replication cycle. (peptides.de)
  • The arteriviruses are highly species specific, but share many biological and molecular properties, including virion morphology, a unique set of structural proteins, genome organization and replication strategy, and the ability to establish prolonged or true persistent infection in their natural hosts. (bionity.com)
  • Both viral and cellular proteins are required for replication and transcription. (bionity.com)
  • In addition, Immunoblot assays were used to detect viral nucleoproteins and various cell signaling proteins involved in the interferon response. (news-medical.net)
  • The antigenomes provide as layouts for the replication resulting in the amplification from the genome whereas the mRNAs are translated into viral proteins. (immune-source.com)
  • Chromosome replication is triggered when a nucleoprotein complex, termed the orisome, assembles, unwinds the duplex DNA, and recruits the proteins required to establish new replication forks. (mdpi.com)
  • Replication and transcription is mediated by an RNA polymerase complex composed of large (L) and phospho- (P) proteins. (wikidoc.org)
  • The group antigens form the viral core structure and are the major proteins which comprise the nucleoprotein core particles. (ukessays.com)
  • Phosphorylation of SARS-CoV-2 nucleoprotein recruits human cytosolic 14-3-3 proteins playing a well-recognized role in replication of many viruses. (diamond.ac.uk)
  • Coronavirus (CoV) genome replication takes place in the cytoplasm in a membrane-protected microenvironment, and starts with the translation of the genome to produce the viral replicase. (bionity.com)
  • The coronavirus nucleoprotein (N) has been reported to be involved in various aspects of virus replication. (reading.ac.uk)
  • Based on these findings, we further confirmed that N30 provided a strong inhibition on the replication of respiratory syncytial virus, coronavirus, enterovirus 71 and a diverse strains of coxsackie B virus. (biomedcentral.com)
  • SARS-CoV-2 Nucleocapsid_2 B7 (HLA-B*07:02) is a linear peptidic epitope studied as part of nucleoprotein from severe acute respiratory syndrome-related coronavirus. (peptides.de)
  • P recruits the viral polymerase to the nucleoprotein-bound viral RNA (N-RNA) via an interaction between its C-terminal domain and the N-RNA complex. (pasteur.fr)
  • It has been suggested that the expression of viral polymerase and NP allows genome replication by stabilization of cRNA replication intermediates and complementary ribonucleoprotein (cRNP) assembly. (ox.ac.uk)
  • Stabilization of influenza virus replication intermediates is dependent on the RNA-binding but not the homo-oligomerization activity of the viral nucleoprotein. (ox.ac.uk)
  • They are targeting the messenger RNA of the nucleoprotein gene of these viruses, then blocking the virus multiplication process. (europa.eu)
  • In addition research into animal viruses has made an important contribution to our understanding of viruses in general, their replication, molecular biology , evolution and interaction with the host. (bionity.com)
  • Our goal is to understand the molecular mechanisms whereby the genomic RNA of influenza-like viruses is, on the one hand, the template for transcription and replication of the viral genome by its RNA-dependent RNA polymerase and, on the other hand, an Achilles' heel, whose recognition as non-self can trigger an innate immune response to counter the viral infection. (embl.org)
  • Viruses need cells for their replication and, therefore, ways to hijack cellular functions. (bvsalud.org)
  • Host inosine-5'- monophosphate dehydrogenase (IMPDH) involved in the synthesis of guanine nucleotides, is known to be a potential target to inhibit the replication of viruses. (biomedcentral.com)
  • The results demonstrated that N30 inhibited the replication of H1N1, H3N2, influenza B viruses, including oseltamivir and amantadine resistant strains in vitro. (biomedcentral.com)
  • We identified the small molecule N30, as an inhibitor of IMPDH, might be a potential candidate to inhibit the replication of various viruses. (biomedcentral.com)
  • However, both RNA binding and homo-oligomerization activities are essential for genome replication. (ox.ac.uk)
  • The preferred DNA substrate mimics an arrested DNA replication fork with unreplicated lagging strand, structurally identical to a product of recombinational repair of a stalled replication fork. (wikipedia.org)
  • Telomeres are nucleoprotein complexes that cap the end of chromosomes to protect DNA from degradation. (ukbiobank.ac.uk)
  • Metabolism of genetic material (replication, transcription, recombination) proceeds at these complexes and is, therefore, significantly affectedby their properties. (muni.cz)
  • During the next four years, the nucleoprotein complexes will be characterised, which form indispensable functional elements of eukaryotic chromosomes - the telomeres. (muni.cz)
  • RNPs are macromolecular complexes composed of the genomic RNA bound to multiple monomers of a nucleoprotein and a single copy of the polymerase. (csic.es)
  • We show that C-terminally truncated Redβ, whilst still able to promote annealing and nucleoprotein filament formation, is unable to mediate homologous recombination. (nature.com)
  • Because the nucleoprotein filament formed with C-terminally truncated Redβ has altered properties, the second C-terminal function could be due to an interaction required for functional filaments. (nature.com)
  • results from their study show the importance of the RAD51 nucleoprotein filament conformation for stimulating DNA pairing by BLM . (medscape.com)
  • Our current goal is to derive models explaining the detailed mechanisms of transcription and replication of the viral genome (vRNA) by influenza-like viral polymerases. (embl.org)
  • With this idea we opened the second major line of our research, the elucidation of the structural basis of transcription and replication mechanisms and for that our plan is to complement structural data with biochemical assays that will allow us to establish the action mechanism. (csic.es)
  • The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication. (genebiosystems.com)
  • Growing evidences support that inhibition of IMPDH decreases intracellular levels of guanosine nucleotides in DNA or RNA synthesis, thereby indirectly inhibits virus replication which requires host guanine nucleotides as raw materials [ 10 , 11 ]. (biomedcentral.com)
  • In a new study, a team of researchers reports that the loss of a father has a significant adverse effect on telomeres, the protective nucleoprotein end caps of chromosomes. (sciencedaily.com)
  • once telomeres are too short, cell replication stops. (sciencedaily.com)
  • One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) was used to detect intracellular and extracellular SARS-CoV-2 ribonucleic acid (RNA) in the infected ALI HBE culture and human monocytes to confirm viral replication. (news-medical.net)
  • Primosomes are nucleoproteins assemblies that activate DNA replication forks. (wikipedia.org)
  • The "replication restart" primosome, defined in Escherichia coli, is involved in the reactivation of arrested replication forks. (wikipedia.org)
  • They are involved in primosome function both at arrested replication forks and at the chromosomal origin. (wikipedia.org)
  • In mammals, functional analysis of the individual RAD51 paralogues in cell lines has shown similar but non-redundant contributions in DNA repair processes such as HR efficiency, RAD51 nuclear focus formation, sensitization to mitomycin C (MMC) and protection of perturbed replications forks [ 11 ]. (nature.com)
  • In 2013, a team of researchers led by Anny Slama-Schwok of Paris Saclay University, used computer modeling to screen a library of compounds for ones that bound to a groove in the nucleoprotein that normally binds RNA, hoping to find something that could inhibit the nucleoprotein's function and act as an antiviral. (acs.org)
  • A defective virus, containing particles of RNA nucleoprotein in virion-like form, present in patients with acute hepatitis B and chronic hepatitis. (bvsalud.org)
  • Therefore, there is an interest to develop a curative tool that could help to control the virus replication when animals are newly infected or are at high risk to become infected in the next days. (europa.eu)
  • The influenza virus nucleoprotein (NP) is believed to play a central role in directing a switch from RNA genome transcription to replication by the viral RNA polymerase. (ox.ac.uk)
  • The virus is the able to initiate a new round of replication again. (ukessays.com)
  • It is also possible that they received a sufficiently large dose of virus to induce antibodies, but that viral replication did not occur. (virology.ws)
  • were able to seroconvert without replication of the virus even having to take place….is that even possible though? (virology.ws)
  • Virus defectuoso que contiene partículas de nucleoproteína ARN en forma de tipo virión, presente en pacientes con hepatitis B aguda y crónica. (bvsalud.org)
  • Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)
  • These studies are being extended to viral RNPs (the physiological RNA synthesis units) to understand the behaviour of the viral nucleoprotein during replication and transcription and to include host factors important for viral replication. (embl.org)
  • Our goal is to solve the polymerase dimer structure that represents the functional complex for replication of RNPs. (csic.es)
  • The trojan replicates in lots of cell types and after uncoating the L M and S RNAs from the nucleoprotein as well as the polymerase by means of RNPs will be the layouts for the formation of two types of cRNA substances the antigenomes as well as the mRNAs. (immune-source.com)
  • Replication and transcription occur in the cell nucleus. (medscape.com)
  • Thogotoviruses undergo replication and transcription within the cell nucleus. (medscape.com)
  • Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
  • During this Marie Curie action, we have identified siRNA sequences capable of inhibiting more than 90 % of the replication of PPRV and RPV in infected cell cultures. (europa.eu)
  • TL shortens with each round of cell replication and is considered a hallmark of biological aging. (ukbiobank.ac.uk)
  • nevertheless, the result of this activation for disease replication or sponsor cell defence can be less very clear (Schultz-Cherry et al. (scienceexhibitions.org)
  • To achieve this we use X-ray crystallography and single-particle cryoEM to determine structures after trapping successive states along the active transcription or replication pathways. (embl.org)
  • Steinman suspected that antibodies the body produced against the nucleoprotein in the vaccine might also stick to hypocretin receptor 2, disrupting its function. (cosmosmagazine.com)
  • MVA-M1+NP vaccine candidate consists of MVA, a replication-deficient pox viral vector that has been safely tested in thousands of patients, to generate a strong immune reaction against Matrix 1 (M1) and Nucleoprotein (NP) influenza antigens. (precisionvaccinations.com)
  • Focetria turned out to have much less nucleoprotein in it - a quarter of that found in Pandemrix - which was not enough to produce antibodies against the hypocretin receptor, Steinman showed. (cosmosmagazine.com)
  • Antibodies against the viral nucleoprotein were found in three individuals, two of whom were also positive for viral neutralizing antibodies. (virology.ws)
  • An application for a French patent entitled 'Interfering RNA targeting the nucleoprotein gene of morbilliviruses' (INPI N°05 13029) was deposited in December 2005 and an extension for an international patent was made one year after (PCT / FR2006 / 002819). (europa.eu)
  • MOKV phosphoprotein P is an essential component of the replication and transcription complex and acts as a cofactor for the viral RNA-dependent RNA polymerase. (pasteur.fr)
  • Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes. (expasy.org)
  • Furthermore, human monocytes were protected from influenza A infections even after an abortive Omicron infection, indicating that complete viral replication is not required for an interferon-mediated antiviral state. (news-medical.net)
  • 2000). Another locating towards an antiviral part of apoptosis can be caspase-mediated cleavage from the influenza viral nucleoprotein (NP) (Zhirnov et al. (scienceexhibitions.org)
  • Obviously, the initiation of chromosome replication is essential to bacterial reproduction, but this process is not inhibited by any of the currently-used antimicrobial agents. (mdpi.com)
  • Bottom row: the docking of the influenza A nucleoprotein atomic structure (pdb 2IQH) is shown in the opposite strands in red and blue. (csic.es)
  • Previous experiments revealed that an Eα fragment, Tα1-Tα2, which constitutes a well-characterized compact nucleoprotein structure led to premature activation of V(D)J recombination compared with that observed for the entire Eα or Tα1-Tα4. (aai.org)
  • In living cells the genome is constantly being damaged by environmental agents, oxidative stress and replication errors 1 . (nature.com)
  • Despite being the target of extensive research efforts due to the COVID-19 pandemic, relatively little is known about the dynamics of SARS-CoV-2 replication within cells. (cam.ac.uk)
  • This suggests that the nucleoprotein assembly is sequential in the PriA, DnaD, DnaB order. (wikipedia.org)
  • These data provide new insights into the spatiotemporal regulation of SARS-CoV-2 assembly, and refine current understanding of SARS-CoV-2 replication. (cam.ac.uk)
  • Modeling shows how the anti-inflammatory naproxen (black) and four derivatives of drug (yellow, cyan, orange and green) fit into the groove of the influenza nucleoprotein, blocking its activity. (acs.org)
  • Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication. (southernbiotech.com)
  • Antisense therapies, in which oligonucleotides inhibit viral replication, have shown promising results in non-human primates following post-exposure treatment. (biomedcentral.com)