• genomic
  • Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. (biomedsearch.com)
  • Reference-free SNP calling: improved accuracy by preventing incorrect calls from repetitive genomic regions. (nih.gov)
  • The advent of next-generation sequencing (NGS) technologies has made it possible to efficiently genotype a large number of SNPs in the non-model organisms with no or limited genomic resources. (nih.gov)
  • The iML algorithm incorporates the mixed Poisson/normal model to detect composite read clusters and can efficiently prevent incorrect SNP calls resulting from repetitive genomic regions. (nih.gov)
  • The iML algorithm can efficiently prevent incorrect SNP calls resulting from repetitive genomic regions, and thus outperforms the original ML algorithm by achieving much higher genotyping accuracy. (nih.gov)
  • The results highlight the promise of next generation sequencing for development of genomic resources for any organism. (nih.gov)
  • probe
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • proteins
  • In eukaryotes, tail-anchored proteins possessing a hydrophobic insertion sequence at their C-terminus are delivered to the endoplasmic reticulum (ER) by the SRP. (wikipedia.org)
  • Regions
  • The red blocks in the left panel indicate alignable regions of the TEs and other repeat sequences. (nih.gov)
  • genomes
  • Through analysis of simulation and real sequencing datasets, we demonstrate that in comparison with ML or a threshold approach, iML can remarkably improve the accuracy of de novo SNP genotyping and is especially powerful for the reference-free genotyping in diploid genomes with high repeat contents. (nih.gov)
  • whereas
  • For A. thaliana, iML always generated lower FPRs than the threshold approach or ML with 12 ~ 19%, 6 ~ 11% and 2 ~ 4% FPR reductions corresponding to the read lengths of 35, 50 and 100 bp, respectively, at a 40x sequencing depth, whereas iML generated only slightly higher FNRs (~1%) in comparison with ML (Figure 3A, Additional file 3: Table S3). (nih.gov)
  • Activation was achieved by transmembrane sequences such as [VVVEVVA]n or [VVVEVVV]n, whereas activation was not achieved by a transmembrane domain consisting only of Val residues. (nih.gov)
  • Some transposases non-specifically bind to any target site in DNA, whereas others bind to specific target sequences. (wikipedia.org)
  • stable
  • Furthermore, retrotransposon-induced mutations are relatively stable, because the sequence at the insertion site is retained as they transpose via the replication mechanism. (wikipedia.org)
  • Analysis
  • This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements. (biomedsearch.com)
  • DNA sequence analysis and hybrid selection and in vitro translation show that molecules of this repeat family are not translatable. (umassmed.edu)
  • repeat
  • Accordingly, a repetitive unit of a particular microsatellite locus is commonly referred to as a mono-, di-, tri-, tetra- or pentanucleotide repeat locus, as applicable. (google.com)
  • motif
  • The five-residue sequence motif found in many receptor tyrosine kinase transmembrane domains (Sternberg and Gullick, 1990), referred to as P0-P4, corresponds to residues 661-665. (nih.gov)
  • clones
  • However, the distinction between the sequences of the transforming clones and the nontransforming clones did not suggest clear rules for predicting which specific sequences would result in receptor activation and transformation. (nih.gov)