• The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. (wikipedia.org)
  • DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences. (neb.com)
  • A tandem repeat region corresponding to 563048-563028 nt of the strain Sheila Smith genome and flanking sequences were amplified with AF (5′-GTGATTGCTATATTTCGCTTT-3′) and AR (5′- CTAAGATTTGTTCCGTATAGG-3′) primers as described elsewhere ( 7 ). (cdc.gov)
  • The 5'-flanking region of the (H + + K + )-ATPase gene contains potential binding sites for RNA polymerase II and various transcriptional regulation factors and several direct and inverted repeat sequences which may be important for specific and controlled expression of the gene in gastric parietal cells. (elsevierpure.com)
  • In addition, we sequenced 99 ospC flanking sequences from different lineages and compared the complete cp26 sequences of 11 strains as well as the cp26 bbb02 loci of 56 samples. (cdc.gov)
  • Based on the amino acid sequences flanking the EPIYA motifs, the EPIYA motifs can be subdivided into four distinct peptide segments: EPIYA-A, EPIYA-B, EPIYA-C and EPIYA-D [ 23 ]. (biomedcentral.com)
  • We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)
  • Sequences flanking these clusters would be unique to one LCR or the other and would be marked by paralogous sequence variants (PSVs). (elsevierpure.com)
  • Several transcription regulatory sequences were identified in the promoter region. (elsevierpure.com)
  • these sequences were referred to as telomeres [ 3-5 ]. (actanaturae.ru)
  • The telomeres consist of repeating sequences and a set of special proteins, which interact with these repeats and spatially organize them in a specific manner, resulting in the formation of the nucleoprotein complex known as telomeric heterochromatin [ 6 , 7 ]. (actanaturae.ru)
  • However, the 5'-flanking regions of the genes for (H + + K + )-ATPase and the (Na + + K + )-ATPase α(+) subunit show no apparent sequence homology, indicating that their transcriptions are regulated differently. (elsevierpure.com)
  • There are two polyadenylation signals in the 3'-flanking region of the (H + + K + )-ATPase gene, but the sequence of this region shows no homology to those of the corresponding regions of the genes for the (Na + + K + )-ATPase α and αIII subunits. (elsevierpure.com)
  • Over time, OspC genes were transferred between strains through recombinations involving the whole or parts of the gene and one or both flanks. (cdc.gov)
  • Acquisitions of OspC genes that are novel for the region confer to recipients unique identities to host immune systems and, presumably, selective advantage when immunity to existing types is widespread among hosts. (cdc.gov)
  • 2017). Promoter Variation and Expression Levels of Inflammatory Genes IL1A, IL1B, IL6 and TNF in Blood of Spinocerebellar Ataxia Type 3 (SCA3) Patients . (up.pt)
  • The ~1.5 Mb candidate region was shown to contain five protein-coding genes. (biomedcentral.com)
  • Retroviruses like FeLV consist of protein-encoding genes flanked by two non-coding regions, LTRs. (vin.com)
  • Commonly, MOI is assessed by using the polymorphic regions of P. falciparum merozoite surface proteins 1 and 2 genes ( msp 1/2 ) as markers. (biomedcentral.com)
  • Six Bacterial Artificial Chromosome (BAC)-end sequence derived SSRs (CaM0717, CaM2086, CaM1760, CaM1364, CaM1122, and CaM0886) are present in this QTL region and can be used for isolation of vernalization response genes. (icrisat.org)
  • Among 84 flowering related genes present in this region, Ca_06280 related to MADS box genes, was reported to play important role in vernalization in cereals. (icrisat.org)
  • Deletions of pfhrp2 and paralogue pfhrp3 (pfhrp2/3) genes threaten Plasmodium falciparum diagnosis by rapid diagnostic test. (cdc.gov)
  • The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality (3). (neb.com)
  • Repeat sequence (GCCTTAT, indicated with brackets) present in 3 copies in strain Sheila Smith, whereas only 2 copies were present in R. rickettsii isolates from Brazil, Colombia, Costa Rica, and Panama. (cdc.gov)
  • Though the VNTR resides in a region encoding the 3' untranslated region (UTR) and does not alter the protein's amino acid sequence, the prevalent 10-repeat variant has shown both linkage and association to Attention Deficit Hyperactivity Disorder (ADHD). (biomedcentral.com)
  • The control region of the fast-twitch sarcoplasmic reticulum Ca 2+ -ATPase gene also showed no sequence homology to that of (H + + K + )-ATPase. (elsevierpure.com)
  • In the present study genetic diversity in 19 commercial varieties and 29 landraces of cluster bean belonging to Gujarat, Rajasthan, Haryana and Delhi regions of India were analyzed using 13 RAPD (Randomly Amplified Polymorphic DNA) and 7 1SSR (Inter Simple Sequence Repeat) markers. (iitr.ac.in)
  • 2012). Sequence analysis of 5′ regulatory regions of the Machado-Joseph disease gene (ATXN3) . (up.pt)
  • We propose to define unstable regions on 22q11.2 by DNA sequence analysis. (elsevierpure.com)
  • The simple sequence repeat (SSR) markers TA64 and CaM1515 were flanking the QTL which spans a distance of 22 cM. (icrisat.org)
  • Microsatellites (short tandem repeats, STR, or simple sequence repeats, SSR) are widely used markers in population genetics. (cd-genomics.com)
  • single‐nucleotide polymorphisms (SNPs) or insertions/deletions (indel) polymorphisms in the nucleotide sequence of that fragment, either within the repetitive array or in the flanking regions (FR), remain undetected by length assessment alone. (cd-genomics.com)
  • more 2021-3-26 · The National Library of Medicine (NLM), on the NIH campus in Bethesda, Maryland, is the world's largest biomedical library and the developer of electronic information services that delivers data to millions of scientists, health professionals … Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. (netlify.app)
  • A series of four DAT1 constructs, each containing the DAT1 coding region, but varying with respect to the downstream presence or content of the 3'UTR, were engineered and stably transfected into an HEK-293 variant using Flp-In integration, an enzyme-mediated, site-specific recombination technology. (biomedcentral.com)
  • Flp-mediated recombination, one of a family of site-specific recombination technologies, was used to integrate the DAT1 constructs into a common, transcriptionally-active region of the genome, eliminating several caveats associated with traditional reporter gene methodologies [ 36 ]. (biomedcentral.com)
  • To facilitate this flexibility, the Cas9 gene is cloned in a region bracketed by two P-element terminal repeats and near an attB recombination site. (vectorbuilder.kr)
  • Non-allelic homologous recombination (NAHR) events between two, 240 kb low copy repeats, termed LCR22-2 and LCR22-4, mapping 3 Mb apart, lead to several genomic disorders including velo-cardio-facial/DiGeorge syndrome (VCFS/DGS), occurring in 1/4,000 live births. (elsevierpure.com)
  • Such regions might serve as recombination hotspots responsible for NAHR events. (elsevierpure.com)
  • The vector pSelAct-KO is a recombination based, markerless knockout system that uses dual selection to engineer seamless deletions of a region of interest, providing opportunities for repeated higher-order genetic knockouts. (biorxiv.org)
  • In most cases, the syndrome is due to a 3 million base pair (Mb) deletion on the chromosomal region 22q11.2 that is flanked by low copy number repeats. (cdc.gov)
  • The long-term goal of our program is to assess the roles in which LCRs confer genome instability using the 22q11.2 region as a model. (elsevierpure.com)
  • PUBLIC HEALTH RELEVANCE: The chromosome 22q11.2 region is associated with multiple developmental disorders. (elsevierpure.com)
  • DESCRIPTION (provided by applicant): Low copy repeats (LCRs;also known as segmental duplications) constitute roughly 5% of the human genome and are known to mediate chromosome rearrangements associated with mental retardation disorders. (elsevierpure.com)
  • The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)
  • In Specific Aim 3, we will test the hypothesis that there are varied regions of historic gene conversion within the LCR22s and will define a set of PSV markers flanking these regions. (elsevierpure.com)
  • The PSV markers will be used in the future to type our cohort of 250 VCFS/DGS patients with the typical 3 Mb deletion and their normal parents to identify signatures of genomic instability. (elsevierpure.com)
  • Marker analysis using 19 polymorphic ISSR markers showed polymorphic bands ranged from 15 to 28 with molecular weight of 100 bp to 3 kb. (biomedcentral.com)
  • The present study utilized radioligand binding and immunoblotting techniques to compare in vitro DAT density and ligand affinity across a series of cell lines stably transfected with constructs containing the DAT1 coding region flanked downstream by one of four DAT1 3'UTR variants. (biomedcentral.com)
  • We performed assays for Plasmodium antigen carriage, pfhrp2/3 genotyping, and sequencing for 7 neutral microsatellites to assess relatedness. (cdc.gov)
  • There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. (biomedcentral.com)
  • A 40-bp variable number of tandem repeats (VNTR) polymorphism exists in the 15th exon of DAT1 , the gene encoding the human dopamine transporter (DAT). (biomedcentral.com)
  • repeated 35 times), a 2 36 bp product from exon 4 of the human S OD1 gene. (netlify.app)
  • Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer. (biomedcentral.com)
  • We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. (elsevierpure.com)
  • This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
  • a promoter region responsible for the negative transcriptional regulation by ABA. (biomedcentral.com)
  • UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)
  • These regions have multiple functions, including regions that promote transcription of regions directly adjacent and far away from the LTRs. (vin.com)
  • BEST Arabidopsis thaliana protein match is: Transducin/WD40 repeat-like superfamily protein (TAIR:AT1G18830.1). (riken.jp)
  • 3 H] Win 35,428 saturation binding assays and DAT immunoblots revealed statistically significant differences in DAT expression attributable to DAT1 genotype. (biomedcentral.com)
  • The aim of this study was to investigate the diversity of cagA 3′ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori , and their association with gastroduodenal diseases. (biomedcentral.com)
  • Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers? (genscript.com)
  • Pelvic fractures account for 1-3% of all skeletal fractures and 2% of orthopedic hospital admissions. (medscape.com)
  • Despite its high frequency in the general population [ 7 ] and an absence of studies addressing possible effects of the VNTR on measures of DAT physiology and pharmacology, the 10-repeat DAT1 allele has become generally recognized as a "high risk" allele for ADHD. (biomedcentral.com)
  • Three overlapping but distinct populations of B. burgdorferi corresponded to the geographic regions. (cdc.gov)
  • Although several efficient therapies for this disease including surgical operation, and the combination of radiotherapy and chemotherapy, have been developed in recent years, the prognosis of patients at advanced stage is still poor [ 3 ]. (hindawi.com)
  • A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. (biomedcentral.com)
  • 3] In his 1966 report on 65 patients with double vertical fractures of the pelvis, R ä f noted a worse outcome when SI dislocation was present. (medscape.com)
  • from Madagascar, 14% of 64 patients with Abdominal sonography showed mul- extrapulmonary tuberculosis had tubercular tiple lymphadenopathies in the para-aor- anal fistula [ 3 ]. (who.int)
  • We examined 1,002 samples from suspected malaria patients in Djibouti City, Djibouti, to investigate pfhrp2/3 deletions. (cdc.gov)
  • 2016). Triplet Repeat Primed PCR (TP-PCR) in Molecular Diagnostic Testing for Spinocerebellar Ataxia Type 3 (SCA3) . (up.pt)
  • The absence or rarity of B. burgdorferi in Ohio and in the Rocky Mountain and Great Basin regions suggests that these three populations of B. burgdorferi are geographically if not ecologically isolated. (cdc.gov)
  • They have analyzed samples from 20 cats from three different 3 populations. (vin.com)
  • Plasmodium vivax infection among Duffy antigen-negative individuals from the Brazilian Amazon region: an exception? (univalle.edu.co)
  • tic and mesenteric regions as well as the Diagnosis of perianal tuberculosis is hilar portion of the liver. (who.int)
  • No clear DNase I footprints are seen with T 15 CGA 15 though alterations in the nuclease susceptibility of surrounding regions suggest that the ligand is binding, albeit weakly at this site. (soton.ac.uk)
  • The only preventive measure available is Mycobacterium bovis Bacillus Calmette-Guerin (BCG), which has fared extremely well in providing protection against childhood TB 3 . (nature.com)
  • 3 times) was negative for Mycobacterium thology results were available from those operations. (who.int)
  • Involvement of the perianal region in tu- men from the anal ulcer was positive for berculosis is a rare extrapulmonary form acid-fast bacilli and Mycobacterium tub of this disease. (who.int)
  • Classification of Persons Exposed to and/or Infected with year more than 8 million new cases of tuberculosis occur and Mycobacterium tuberculosis approximately 3 million persons die from the disease (1). (cdc.gov)
  • Torn chromosomes can fuse with each another, rearrange, and they are characterized by instability [ 2 , 3 ]. (actanaturae.ru)
  • T ie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. (rndsystems.com)
  • Regions of A n and T n are rendered much more reactive to DNase I and adenines on the 3′-side of the CG become hyperreactive to diethylpyrocarbonate. (soton.ac.uk)
  • These regions are flanked on either side by a long terminal repeat (LTR) region. (pharmacy180.com)
  • Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). (rndsystems.com)
  • Human Heredity, 60 (3), 156 - 163. (up.pt)
  • Another problem is the involuntary cross-contamination that can promote adulteration of products or contaminating agents caused by sharing equipment between different plant and animal species or due to unhygienic human handling practices [3]. (peertechzpublications.com)
  • in Adults and Children" is a joint statement prepared by the In the United States, an estimated 15 million people are in- American Thoracic Society and the Centers for Disease Con- fected with M. tuberculosis (3). (cdc.gov)
  • Regions of alternating AT show unusual changes in the presence of the ligand. (soton.ac.uk)
  • DNase I footprinting experiments performed at 4°C revealed neither enhancements nor footprints for flanking regions of homopolymeric A and T suggesting that the conformational changes are a necessary consequence of drug binding. (soton.ac.uk)
  • Chiu ES , Gagne RB, Lee JS, and VandeWoude S. Characterization of Endogenous Feline Leukemia Virus (enFeLV) Long Terminal Repeat (LTR) Integration Site Diversity. (vin.com)
  • Subsequently, numerous gene families arising as a result of expansions from a single gene have been identified [ 3 ]. (biomedcentral.com)
  • L1 retrotransposons preferentially accumulated in the Abp gene regions after lineage separation and roughly followed the pattern of Abp gene expansion. (biomedcentral.com)
  • We identified 34 visual behaviours, which were grouped into eight categories according to their functional character: food = 4), social interaction (n = 6), displacement (n = 2), resting (n = 3), grooming (n = 4), neutral position (n = 3), bipedal (n = 5) and other activities (n = 7). (bvsalud.org)
  • Almost all bladder cancers originate in the urothelium, which is a 3- to 7-cell mucosal layer within the muscular bladder. (medscape.com)
  • it constitutes more than 90% of bladder cancers in those regions. (medscape.com)
  • At low concentrations (5μM) cleavage of TpA is enhanced, whereas at higher concentrations a cleavage pattern with a four base pair repeat is evident. (soton.ac.uk)
  • Histidine-rich protein 2/3 antigen concentrations were reduced with corresponding gene deletions. (cdc.gov)
  • In regions where B. burgdorferi is enzootic, sites as small as a few hectares have between 9 and 15 strains ( 4 - 6 ). (cdc.gov)