• cytotoxicity
  • Anti-CD70 antibody-drug conjugates (ADC) consisting of auristatin phenylalanine phenylenediamine (AFP) or monomethyl auristatin phenylalanine (MMAF), two novel derivatives of the anti-tubulin agent auristatin, mediated potent antigen-dependent cytotoxicity in CD70-expressing RCC cells. (aacrjournals.org)
  • The efficiency of drug delivery using anti-CD70 as vehicle was illustrated by the much enhanced cytotoxicity of antibody-conjugated MMAF compared with free MMAF. (aacrjournals.org)
  • tumor cell
  • Hence, ADCs targeted to CD70 can selectively recognize RCC, internalize, and reach the appropriate subcellular compartment(s) for drug release and tumor cell killing. (aacrjournals.org)
  • disease
  • CRC screening can possibly reduce the incidence of advanced disease and provide better overall, progression-free survival. (omicsonline.org)
  • Identifying new markers for disease progression and drug targets for RCC will benefit this unmet medical need. (aacrjournals.org)
  • Since sequencing of the human genome which allowed rapid cloning and synthesis of large quantities of purified proteins, it has become common practice to use high throughput screening of large compounds libraries against isolated biological targets which are hypothesized to be disease modifying in a process known as reverse pharmacology. (wikipedia.org)
  • activity
  • Especially, the paper focused on the relevant mechanism study of EAC-B against MGC-803 included cell cycle distribution (flow cytometry) and cyclin D1 expression (RT-PCR and western blot), apoptosis (Hoechst 33342 stain and flow cytometry), apoptosis-related protein expression (Akt, p-Akt, ERK, p-ERK, Bcl-2, Bax) by western blot, and caspase3/7 activity assay. (biomedcentral.com)
  • Hairy cell leukemia typically shows high levels of CD22 expression but the drug also showed signs of activity in other indications, such as chronic lymphocytic leukemia. (aacrjournals.org)
  • The idea that the effect of a drug in the human body is mediated by specific interactions of the drug molecule with biological macromolecules, (proteins or nucleic acids in most cases) led scientists to the conclusion that individual chemicals are required for the biological activity of the drug. (wikipedia.org)
  • Historically, substances, whether crude extracts or purified chemicals, were screened for biological activity without knowledge of the biological target. (wikipedia.org)
  • involves
  • Discovering drugs that may be a commercial success, or a public health success, involves a complex interaction between investors, industry, academia, patent laws, regulatory exclusivity, marketing and the need to balance secrecy with communication. (wikipedia.org)
  • resistance
  • However, drug resistance frequently happens due to additional mutation on EGFR, such as substitution from threonine to methionine at amino acid position 790 (T790M). (mdpi.com)
  • In this study, we screened a traditional Chinese medicine (TCM) compound library consisting of 800 single compounds in TKI-resistance NSCLC H1975 cells, which contains substitutions from leucine to arginine at amino acid 858 (L858R) and T790M mutation on EGFR. (mdpi.com)
  • effect
  • Meanwhile, for disorders whose rarity means that no large commercial success or public health effect can be expected, the orphan drug funding process ensures that people who experience those disorders can have some hope of pharmacotherapeutic advances. (wikipedia.org)
  • discovery
  • 3-7 As FIMM's Tea Pemovska and colleagues wrote in Drug Discovery World last summer, "coupling of genomic and molecular profiling of cancer with functional testing of drugs in patient-derived cell samples will make it possible to customize patient treatments as well as to identify biomarker patterns and genetic aberrations that explain the drug responses. (personalizedmedonc.com)
  • The significance of healing property herbs has been highly evaluated in drug discovery. (biomedcentral.com)
  • Historically, drugs were discovered through identifying the active ingredient from traditional remedies or by serendipitous discovery. (wikipedia.org)
  • molecular
  • Our partners at the Institute for Molecular Medicine (FIMM) at the University of Helsinki, Finland, have been at the forefront of this application of HT screening, as shown by recent publications. (personalizedmedonc.com)
  • Background: Optimal molecular markers for detecting colorectal cancer (CRC) in a blood-based assay were evaluated. (omicsonline.org)
  • provide
  • 1 mL of SHP647 sterile aqueous buffered solution at an appropriate concentration to provide the intended dose of drug (25 or 75 mg). (clinicaltrials.gov)
  • tests
  • Conventional CRC screening tests include fecal occult blood testing, flexible sigmoidoscopy, doublecontrast barium enema X-ray, and colonoscopy [ 3 ]. (omicsonline.org)
  • score
  • Remission is defined as a composite score of patient-reported symptoms using daily e-diary and centrally read endoscopy as stool frequency subscore of 0 or 1 with at least a 1-point change from baseline, rectal bleeding subscore of 0 and endoscopic subscore of 0 or 1 (modified, excludes friability). (clinicaltrials.gov)
  • natural
  • Fortunately, Paclitaxel (Taxol) is proved to be a successful drug extracted from natural product to answer the call to fill the need. (biomedcentral.com)
  • small
  • Later, small molecules were synthesized to specifically target a known physiological/pathological pathway, avoiding the mass screening of banks of stored compounds. (wikipedia.org)
  • process known
  • Mitomycin C (MMC) is a clinically used anticancer drug that is reduced to cytotoxic metabolites by cellular reductases via a process known as bioreductive drug activation. (aacrjournals.org)
  • line
  • Among four fractions, the IC50s of n-BuOH fraction of EAC (EAC-B) against the five cell lines and time-dependent inhibition to gastric cancer cell line (MGC-803) were further investigated (MTT assay). (biomedcentral.com)
  • ability
  • The ability to use primary tumor tissues, as opposed to immortalized cell lines, is relatively straightforward in the miniaturized assays supported by acoustic liquid handling. (personalizedmedonc.com)
  • active
  • It should be noted that each 200 mg tablet contains only 155 mg of active drug. (medscape.com)
  • Examples of drug compounds isolated from crude preparations are morphine, the active agent in opium, and digoxin, a heart stimulant originating from Digitalis lanata. (wikipedia.org)