• In biochemistry, globular proteins or spheroproteins are spherical ("globe-like") proteins and are one of the common protein types (the others being fibrous, disordered and membrane proteins). (wikipedia.org)
  • Globular proteins are somewhat water-soluble (forming colloids in water), unlike the fibrous or membrane proteins. (wikipedia.org)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • Their unique hourglass-shaped architecture is highly conserved among different bacterial membrane proteins and other biological channels. (ibecbarcelona.eu)
  • Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
  • ATP-binding cassette (ABC) proteins play important roles in cells as importers and exporters but as membrane proteins they are subject to well-known challenges of isolating pure and stable samples for study. (diamond.ac.uk)
  • Globular proteins are only marginally stable because the free energy released when the protein folded into its native conformation is relatively small. (wikipedia.org)
  • As the free energy difference in a globular protein that results from folding into its native conformation is small, it is marginally stable, thus providing a rapid turnover rate and effective control of protein degradation and synthesis. (wikipedia.org)
  • Shortly after SARS-CoV emerged at the turn of the 21st century, the spike (S) protein (particularly in its native conformation) was identified as the immunodominant antigen of the virus3. (who.int)
  • In each case, the amino acid substitution only leads to small perturbations in the conformation of the protein. (ubc.ca)
  • The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). (biolegend.com)
  • SARS-CoV-2 B.1.1.7 Spike Protein S1, amino acid Val16-Arg685 (Accession # QHD43416.1), with (HV69-70del, Y144del, N501Y, A570D, P681H) and a C-terminal 8-His tag was expressed in CHO cells. (biolegend.com)
  • The 678 amino acid recombinant protein has a predicted molecular mass of approximately 76 kD. (biolegend.com)
  • The biologically important organic compound known as amino acids is responsible for building protein in the body. (uticahistoricalsociety.org)
  • Proteins consist of 20 types of amino acids, some of which are called standard and others are nonstandard. (uticahistoricalsociety.org)
  • Nonstandard amino acids have different side chains, and are often altered after being incorporated into 단백질 보충제 protein. (uticahistoricalsociety.org)
  • For example, the amino acid g-carboxyglutamic acid is found in the blood-clotting protein prothrombin. (uticahistoricalsociety.org)
  • Until now, protein turnover has been measured only indirectly, based on serial muscle biopsies and the infusion of stable isotope-labeled amino acids. (uticahistoricalsociety.org)
  • Cysteines are one of the most rarely used amino acids, but when conserved in proteins they often play critical roles in structure, function, or regulation. (hindawi.com)
  • Cysteine is the most important redox-responsive amino acid within proteins largely due to the wide range of oxidation states that sulfur can occupy-so called, "sulfur switches" [ 4 ]. (hindawi.com)
  • All the estimated nutritional parameters based on amino acids composition suggested that Nile tilapia protein hydrolysates and concentrates have good nutritional quality and could be used as protein ingredient in food industries. (scialert.net)
  • To accelerate the translocation of mRNA-tRNAs through the ribosome, bacterial elongation factor G (EF-G) hydrolyzes energy-rich guanosine triphosphate (GTP) for every amino acid incorporated into a protein. (yale.edu)
  • An essential branched-chain aliphatic amino acid found in many proteins . (lookformedical.com)
  • Twenty alpha-amino acids are the subunits which are polymerized to form proteins . (lookformedical.com)
  • The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS . (lookformedical.com)
  • An unnatural amino acid that is used experimentally to study protein structure and function. (lookformedical.com)
  • The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. (lookformedical.com)
  • Enzymes (and other proteins) are composed of amino acid chains called polypeptide chains. (newworldencyclopedia.org)
  • Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. (cipsm.de)
  • This phenomenon can be explained if Cdcp4 has more than one essential function or, alternatively, if two mutant proteins assemble to form a functional complex. (ubc.ca)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • Hence, TADs are believed to represent structural chromosomal units that are of functional importance for the regulatory cross talk that determines gene expression programs. (biomedcentral.com)
  • For this reason, protein structures are considered to be aperiodic, and may have evolved this way for functional purposes, especially in instances that require a combination of softness and rigidity within the same molecule. (preprints.org)
  • Human NAD(P)H:quinone oxidoreductase 1 (NQO1) is a multi-functional protein whose alteration is associated with cancer, Parkinson´s and Alzheimer´s diseases. (preprints.org)
  • NQO1 displays a remarkable functional chemistry, capable of binding different functional ligands that modulate its activity, stability and interaction with proteins and nucleic acids. (preprints.org)
  • Indeed, it has been demonstrated that cysteines are characterized by the most extreme conservation pattern, being highly conserved in functional positions of proteins but poorly conserved otherwise [ 5 ]. (hindawi.com)
  • Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
  • Protein functional properties are determined to a large extent by a protein s physicochemical and structural properties ( Diniz and Martin, 1997 ). (scialert.net)
  • Functional proteins that do not have unique, stable, folded, three-dimensional native structures or that possess non-ordered regions under physiological conditions. (bvsalud.org)
  • Alexander V. Lazarev, Ph.D., Chief Science Officer of PBI, said: "Our collaboration with RedShiftBio takes advantage of our ability to manipulate protein structure with high pressure and RedShiftBio's precise analytical platform that reports subtle changes in the three-dimensional structure of protein molecules that could lead to protein aggregation. (redshiftbio.com)
  • Various cytokines, transcription factors, and other regulatory molecules have been found to be differentially methylated in CD8+ T cells in autoimmune diseases. (frontiersin.org)
  • Such proteins, which resist mechanical forces in some regions while being flexible in others, transmit signals from one region of the protein to another (action at a distance) in response to binding of ligands (oxygen, ATP or other small molecules). (preprints.org)
  • Interactions with other proteins or molecules are another feature of cysteines that can affect protein activity. (hindawi.com)
  • As novel regulatory molecules, circRNAs mediate the regulation of viral infections and the cellular immune response, which provide a new perspective for understanding virus-cell interactions. (biomedcentral.com)
  • The complement system, interacting with its regulatory molecules and cellular receptors, plays a central role in the induction and regulation of immunity. (medscape.com)
  • They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • Part of the protein folding problem is that several non-covalent, weak interactions are formed, such as hydrogen bonds and Van der Waals interactions. (wikipedia.org)
  • Based on the structure of Cdc4p, possible models for interallelic complementation including interactions with partner proteins and the formation of a myosin complex with Cdc4p fulfilling the role of both an essential and regulatory light chain are proposed. (ubc.ca)
  • The aim of the project was to characterize intrachain and interchain interactions involved in the regulatory behaviour of a model allosteric enzyme of known 3-dimensional structure: aspartate carbamoyltransferase (ATCase) of Escherichia coli, a protein consisting of distinct catalytic and regulatory subunits. (europa.eu)
  • High-resolution structures of GPR56 and LPHN3 in their active, G-protein-coupled states, reveal that after dissociation of the extracellular region, the decrypted TA peptides engage the seven-transmembrane domain core with a notable conservation of interactions that also involve extracellular loop 2. (stanford.edu)
  • Moreover, iron interactions with N-terminally acetylated α S, the physiologically relevant form of the human protein, will be addressed to shed light on the current understanding of protein dynamics and the physiological environment in the disease state. (hindawi.com)
  • They are characterized by extraordinary structural flexibility and plasticity, which enable them to adopt different conformations in response to different stimuli or different interactions. (bvsalud.org)
  • Unlike fibrous proteins which only play a structural function, globular proteins can act as: Enzymes, by catalyzing organic reactions taking place in the organism in mild conditions and with a great specificity. (wikipedia.org)
  • Nearly all enzymes with major metabolic functions are globular in shape, as well as many signal transduction proteins. (wikipedia.org)
  • Understanding at the molecular level the conformational transitions underlying catalysis and the transmission of regulatory signals in enzymes is a major goal in fundamental science whereas, in biotechnology, such knowledge is required to lay out the principles of a rational protein design. (europa.eu)
  • This question is investigated in a family of proteins, including enzymes from highly thermophilic bacteria, a potential source of thermostable biocatalysts. (europa.eu)
  • This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
  • For instance, many household cleaners use enzymes to speed up the breakdown of protein or starch stains on clothes. (newworldencyclopedia.org)
  • Moreover, MMS will help us to understand protein folding kinetics in our BaroFold platform, which uses high pressure to shape recombinant proteins into their active and stable conformations in biomanufacturing process development. (redshiftbio.com)
  • However, molecular analyses of T1r have been hampered due to the difficulties in recombinant expression and protein purification, and thus little is known about mechanisms for taste perception. (nature.com)
  • When recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1 is immobilized at 2 μg/mL, recombinant human ACE2-Fc Chimera (Cat. (biolegend.com)
  • Recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1 was aliquoted in PBS at 0.2 mg/mL. (biolegend.com)
  • Loss of Dilp8-Lgr3 signaling renders flies incapable of detecting growth perturbations and thus maintaining a stable size and symmetry. (cell-stress.com)
  • Researchers then analysed these samples to measure the incorporation of the tracer into specific muscle protein fractions and individual proteins. (uticahistoricalsociety.org)
  • Redox proteomics has taken advantage of the thiol specificity of ICAT reagents not only to identify targets of ROS but also to quantify oxidative thiol modifications in individual proteins. (hindawi.com)
  • and the catalytic conformation that truncated INO80DEUBAD lacks to inhibit UCH-L5. (ks-vpeptide.com)
  • RiboNucleic Acids (RNAs) are ubiquitous macromolecules within biological systems, capable of performing a wide range of regulatory and catalytic functions. (dagstuhl.de)
  • It consists of 2 catalytic (C) trimers held together by 3 dimers of regulatory (R) subunit which is itself composed of an effector binding domain and a Zinc binding domain. (europa.eu)
  • The results obtained show that the R1-C4 interaction is essential for the establishment of the enzyme conformation having low affinity for aspartate (T state), and consequently for the existence of cooperactivity between the catalytic sites. (europa.eu)
  • Studies using a derivative of chromosome conformation capture (3C) known as 'Hi-C' have revealed that long-range genomic contacts segregate TADs into an active (A) and inactive (B) compartment [ 37 ]. (biomedcentral.com)
  • Protein synthesis is crucial for cell growth and survival yet one of the most energy-consuming cellular processes. (yale.edu)
  • How, then, do cells sustain protein synthesis under starvation conditions when energy is limited? (yale.edu)
  • EF-G2's singular ability to sustain protein synthesis, albeit at slow rates, is crucial for bacterial gut colonization. (yale.edu)
  • Moreover, we uncover a 26-residue region unique to EF-G2 that is essential for protein synthesis, EF-G2 dissociation from the ribosome, and responsible for the absence of GTPase activity. (yale.edu)
  • Our findings reveal how cells curb energy consumption while maintaining protein synthesis to advance fitness in nutrient-fluctuating environments. (yale.edu)
  • A transfer RNA which is specific for carrying leucine to sites on the ribosomes in preparation for protein synthesis. (lookformedical.com)
  • The histone H2A.F/Z (H2AZ) variant is a functionally distinct, highly conserved histone subgroup that likely represents a separate evolutionary lineage of histone H2A proteins. (sdbonline.org)
  • Hexokinase IV/D) activator that reversibly targets a allosteric site that is only exposed for binding in the glucose-bound GK conformation, but not in the unbound 'super-open' GK conformation or the GKRP- (GK Regulatory Protein) bound GK, and effectively stabilizes GK in an active conformation as well as prevents its interaction with and nuclear sequestration by GKRP, while exhibiting little effect against Hexokinase I/II/III. (sigmaaldrich.com)
  • Following this, the differentially expressed genes (DEGs) were included in Gene Ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein‑protein interaction network and survival analyses. (cancerindex.org)
  • Allosterically regulated proteins that require an activator are sometimes based on a thiol exchange interaction involving cysteines, for example, pyruvate kinase uses fructose bisphosphate (FBPs) as a heterotrophic activator and it contains a cysteine in its FBP binding site [ 8 ]. (hindawi.com)
  • From similar experiments, it appears that the contact between the carboxyl terminal region of the R chain and the 240s loop of the C chain (R1-C4 interaction) is essential for the transmission of the CTP regulatory signal. (europa.eu)
  • The biological function of the differentially expressed circRNAs was determined by protein interaction network, Gene Ontology (GO), and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment. (biomedcentral.com)
  • Regulatory roles are also performed by globular proteins rather than fibrous proteins. (wikipedia.org)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Silencing at mating locus HMR requires silencers, and one of the roles of the silencer is to recruit Sir proteins. (sdbonline.org)
  • Globular proteins seem to have two mechanisms for protein folding, either the diffusion-collision model or nucleation condensation model, although recent findings have shown globular proteins, such as PTP-BL PDZ2, that fold with characteristic features of both models. (wikipedia.org)
  • The joint development work undertaken by PBI and RedShiftBio offers a workflow that allows scientists to study mechanisms of protein aggregation and perturb protein structure "on demand" to promote aggregation events for accelerated stability tests. (redshiftbio.com)
  • Ability to bind to a receptor is influenced by external factors as well as by intracellular regulatory mechanisms. (msdmanuals.com)
  • The Gamblin laboratory studies the mechanisms that lead to the polymerization of the microtubule-associated protein tau. (utsa.edu)
  • Our method allows for the identification of the proteins, identification of redox-sensitive cysteines within proteins, and quantification of the redox status of individual cysteine-containing peptides. (hindawi.com)
  • While the general sequence of SNARE complex formation is well-established, our knowledge on possible intermediates and stable off-pathway complexes is incomplete. (nature.com)
  • In proteins where cysteine is not within the active site, activity can be modulated by changing conformation or by influencing its regulatory role, for example, iron sulfur complexes (ISCs) in aconitase possess cysteines required for its activity [ 7 ]. (hindawi.com)
  • From Protein Complexes to Subunit Backbone Fragments: a Novel Approach to Native Mass Spectrometry. (chicagobiomedicalconsortium.org)
  • The resulting protein forms a mixed disulfide conformation containing free cysteine thiolate and the attacking L-cysteine. (uticahistoricalsociety.org)
  • Reversible cysteine modifications allow for potential redox regulation of proteins. (hindawi.com)
  • Thus, reversible cysteine modifications can influence protein activity and the relative quantification of the status of the thiol can potentially provide valuable insights into protein activity where the protein exists in a range of redox states. (hindawi.com)
  • Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
  • Such an approach would allow biopharmaceutical companies to test specific chemical formulations that stabilize the protein drug and prevent its aggregation. (redshiftbio.com)
  • Molecular analysis suggested that Esc2p contributed to silencing in a manner similar to Sir1p and probably helped recruit or stabilize the other Sir proteins, while Htz1p present at HMR assembled a specialized chromatin structure necessary for silencing (Dhillon, 2000). (sdbonline.org)
  • T1rs are class C G-protein coupled receptors (GPCRs), and the extracellular ligand binding domains (LBDs) of T1r1/T1r3 and T1r2/T1r3 heterodimers are responsible for binding of chemical substances eliciting umami or sweet taste. (nature.com)
  • The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. (biolegend.com)
  • Despite representing one of the largest classes of therapeutic targets, most inactive-state G protein-coupled receptors (GPCRs) have remained inaccessible for cryo-EM because their small size and membrane-embedded nature impedes projection alignment for high-resolution map reconstructions. (stanford.edu)
  • Adhesion G-protein-coupled receptors (aGPCRs) are characterized by the presence of auto-proteolysing extracellular regions that are involved in cell-cell and cell-extracellular matrix interactions1. (stanford.edu)
  • Activated receptors directly or indirectly regulate cellular biochemical processes (eg, ion conductance, protein phosphorylation, DNA transcription, enzymatic activity). (msdmanuals.com)
  • Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)
  • Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)
  • Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
  • Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
  • The accumulation of the intrinsically disordered protein alpha-synuclein (αSyn) in the form of insoluble fibrillar aggregates in the central nervous system is linked to a variety of neurodegenerative disorders such as Parkinson's disease, Lewy body dementia, and multiple system atrophy. (bvsalud.org)
  • The major constituent of LBs is aggregated α -synuclein ( α S), an intrinsically unfolded protein predominantly localized in the presynaptic regions of dopaminergic neurons. (hindawi.com)
  • BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. (elifesciences.org)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • These genes are involved in T cell regulation, including interferons, interleukin (IL),tumor necrosis factor (TNF), as well as linker for activation of T cells (LAT), cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), and adapter proteins. (frontiersin.org)
  • Overexpression of Rv0494 in Mycobacterium bovis BCG reduced the basal level expression of kas operon genes, thereby suggesting the repressor nature of this protein in fatty acid synthase II regulation. (microbiologyresearch.org)
  • We found 1100 differentially expressed circRNAs (675 downregulated and 425 upregulated) which were involved in various biological processes such as protein ubiquitination modification, cell cycle regulation, RNA transport, and autophagy. (biomedcentral.com)
  • Our data show that the stable knockdown of HIPK2 led to wtp53 misfolding, as detected by p53 immunoprecipitation with conformation-specific antibodies, and that p53 protein misfolding impaired p53 DNA binding and transcription of target genes. (aacrjournals.org)
  • We present evidence that zinc supplementation to HIPK2i cells increased p53 reactivity to conformation-sensitive PAb1620 (wild-type conformation) antibody and restored p53 sequence-specific DNA binding in vivo and transcription of target genes in response to Adriamycin treatment. (aacrjournals.org)
  • To exert stimulatory or repressive effects on transcription, accessible regulatory DNA elements must be in close spatial proximity to susceptible genes. (biomedcentral.com)
  • We are testing the hypothesis that specific transcription factors form regulatory networks to execute gene expression programs important for SSC fate decisions (self-renewal and differentiation), and ultimately, spermatogenesis. (utsa.edu)
  • In some processes, chromatin modifications carry heritable regulatory information that is transmitted from mother to daughter cells, whereas in other cases, they are implicated in the execution of the information contained in the DNA sequence, or occur as a consequence of dynamic nuclear processes such as transcription. (plos.org)
  • Muscle protein turnover is governed by the balance of opposing kinetic processes and is essential for the efficient repair of damaged proteins. (uticahistoricalsociety.org)
  • The lifetime of the drug-receptor complex is affected by dynamic processes (conformation changes) that control the rate of drug association and dissociation from the target. (msdmanuals.com)
  • These new findings have shown that the transition states of proteins may affect the way they fold. (wikipedia.org)
  • The receptor activation mechanism of the class A GPCR members, consisting solely of the transmembrane region, has been considered to occur via agonist binding, which changes the conformational dynamics of the protein by lowering the transition energy between the different states, and results in the transition towards the active-state conformation 9 . (nature.com)
  • The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition. (biolegend.com)
  • Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. (lookformedical.com)
  • We argue that non-zero values of $P_2$, leads to an arrangement of the SSEs that can resist mechanical forces, which is a requirement for allosteric proteins. (preprints.org)
  • Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. (cipsm.de)
  • Figure 1: Taste Receptor T1r Proteins from Medaka Fish (mf). (nature.com)
  • To achieve this, the neuronal SNARE (i.e., soluble N -ethylmaleimide-sensitive factor attachment protein receptor) complex brings the vesicle and presynaptic membranes in close proximity, thereby, mediating the fusion of the two membranes resulting in exocytosis of neurotransmitters. (nature.com)
  • Almost all conventional mature T lymphocytes express the IL-7 receptor (IL-7R), with the exception for naturally-occurring regulatory T-cells (Treg), constituting a rare opportunity to selectively strangle pathogenic effectors while preserving crucial natural regulators. (cell-stress.com)
  • A major challenge in the manufacture of biopharmaceuticals is the development of drug formulations that ensure proteins maintain their activity during shelf life and do not aggregate (stick together), since protein aggregation can result in significant issues in patients, sometimes even severe immune reactions up to anaphylactic shock. (redshiftbio.com)
  • Unfortunately, aggregated protein biologics could train a patient's immune system to recognize and eliminate the drug, so finding appropriate formulations to prevent aggregation is a major task in biopharmaceutical drug development. (redshiftbio.com)
  • The co-chaperone Hep1 is required to prevent the aggregation of mitochondrial Hsp70 proteins. (cipsm.de)
  • A tetrameric form of α S has also been identified in human tissues that has been postulated as the inactive storage form of this structurally dynamic protein due to its aggregation resistance [ 6 , 7 ]. (hindawi.com)
  • The SARS-CoV-2 virus can also use its surface 'spike protein' to infect other cells in the body. (health.mil)
  • The spike protein of SARS-CoV--a target for vaccine and therapeutic development. (who.int)
  • TA binding stabilizes breaks in the middle of transmembrane helices 6 and 7 that facilitate aGPCR coupling and activation of heterotrimeric G proteins. (stanford.edu)
  • The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
  • The hierarchical levels of genome architecture exert transcriptional control by tuning the accessibility and proximity of genes and regulatory elements. (biomedcentral.com)
  • This is the first report, to the best of our knowledge, of a GntR/FadR family protein acting as a fatty acid-responsive transcriptional regulator in M. tuberculosis , suggesting a possible role for this protein in mycolic acid biosynthesis. (microbiologyresearch.org)
  • Within an individual protein there may be a number of cysteines which could allow for multiple thiol modifications. (hindawi.com)
  • Proteins synthesized at the ER are subject to covalent modifications that include N- and O-glycosylation, disulfide bond formation, and in some cases, proline and lysine hydroxylation. (stanford.edu)
  • By using NMR spectroscopy, we demonstrate that purified Cdc4p is a monomeric protein with two structurally independent domains, each exhibiting a fold reminiscent of the EF-hand class of calcium-binding proteins. (ubc.ca)
  • The term globin can refer more specifically to proteins including the globin fold. (wikipedia.org)
  • Structural proteins, e.g., actin and tubulin, which are globular and soluble as monomers, but polymerize to form long, stiff fibers Among the most known globular proteins is hemoglobin, a member of the globin protein family. (wikipedia.org)
  • Mechanically, m6A can be recognized by "reader" proteins or can directly modify RNA conformation, and it widely affects gene expression by mediating RNA stability, translation, splicing and export. (scienceopen.com)
  • As a primary sequence of a polypeptide chain can form numerous conformations, native globular structure restricts its conformation to a few only. (wikipedia.org)
  • Letzibeth Mendez-Rivera, a lab manager with The Emerging Infectious Disease Branch at the Walter Reed Army Institute of Research in Silver Spring, Maryland, studies coronavirus protein samples this summer. (health.mil)
  • These methods dramatically increase the likelihood of producing proteins that cannot fold or assemble appropriately. (cipsm.de)
  • Here we show that m6A is selectively recognized by the human YTH domain family 2 (YTHDF2) protein to regulate mRNA degradation. (scienceopen.com)
  • Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
  • This is especially important in reference to redox proteins that contain thiol switches susceptible to activation or inactivation. (hindawi.com)
  • The Bcl-2 family of proteins are the regulators of the mitochondrial pathway of apoptosis and appears responsive to UV-C radiation. (bvsalud.org)
  • SARS-CoV-2 virus, like other viruses, is an infectious agent that consists of genetic coding material called RNA [usually a single-stranded ribose sugar phosphate] or DNA [usually a double-stranded deoxyribose sugar phosphate] that is contained in a protein coat, Ryan stated. (health.mil)
  • We show that NQO1apo has a minimally stable folded core holding the protein dimer and with FAD and dicoumarol ligand binding sites populating binding non-competent conformations. (preprints.org)
  • The term globular protein is quite old (dating probably from the 19th century) and is now somewhat archaic given the hundreds of thousands of proteins and more elegant and descriptive structural motif vocabulary. (wikipedia.org)
  • Binding of FAD significantly decreases protein dynamics and stabilizes the FAD and dicoumarol binding sites as well as the monomer:monomer interface. (preprints.org)
  • The SNARE complex assembles from the vesicular protein Synaptobrevin-2 as well as SNAP25 (synaptosome-associated protein of 25 kDa) and Syntaxin-1, which are both anchored to the presynaptic membrane 2 . (nature.com)
  • Positive Darwinian natural selection can bring about an increase in either component, and negative or stabilizing selection in protein evolution can lead to decreases. (pharmaceuticalintelligence.com)
  • The Company believes combining both platforms enables the rigorous investigation of protein structure and stability in a streamlined and highly efficient workflow that can be critical in drug development. (redshiftbio.com)
  • Julien Bradley, Chief Executive Officer of RedShiftBio, commented: "Accelerated stability studies are an important component of drug development and regulatory submissions. (redshiftbio.com)
  • The Schizosaccharomyces pombe Cdc4 protein is required for the formation and function of the contractile ring, presumably acting as a myosin light chain. (ubc.ca)
  • It is unknown how the structure and, effectively, the function of these proteins are directly impacted by UV-C exposure. (bvsalud.org)
  • Here, we present the effect of UV-C irradiation on the structure and function of pro-apoptotic Bid-FL and anti-apoptotic Bcl-xlΔC proteins. (bvsalud.org)
  • Homeodomain-interacting protein kinase-2 (HIPK2) is a positive regulator of p53 oncosuppressor function. (aacrjournals.org)
  • Tau is a protein that is important in neuronal function, but can misfold and aggregate into pathological structures that accumulate in neurodegenerative disorders such as Alzheimer's disease. (utsa.edu)
  • Further, our NMR studies reveal that these compounds interact with the N-terminal region of αSyn which is unstructured even in the fibrillar form of the protein and is known as the "fuzzy coat" of fibrils. (bvsalud.org)
  • The protein is then remodeled to a more flexible form by a reducing agent. (uticahistoricalsociety.org)
  • Cysteines often form part of active sites, allowing for the protein to be switched on or off depending on redox state. (hindawi.com)
  • Instead, damaged or incorrectly synthesized proteins must be rapidly and efficiently destroyed lest they form toxic aggregates. (stanford.edu)
  • HbFe(III)-OOradical dot, HbFe(III)-OONO and protein-caged [HbFe(III)-Oradical dotradical dotNO2] are proposed intermediates in a reaction mechanism that combines both O-atoms of O2 with radical dotNO to form nitrate and HbFe(III). (pharmaceuticalintelligence.com)
  • Albumins are also globular proteins, although, unlike all of the other globular proteins, they are completely soluble in water. (wikipedia.org)
  • Fusion of synaptic vesicles with the presynaptic membrane occurs in the presence of the NSF (N-ethylmaleimide-sensitive-factor) / α-SNAP (α-soluble NSF attachment protein) disassembly machinery. (nature.com)
  • This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells. (cipsm.de)
  • To some, it suggests that quantum effects are at work even at the large distances (by atomic standards) spanned by a protein molecule. (newworldencyclopedia.org)
  • The etiology of Parkinson's disease (PD) is linked with cellular inclusions in the substantia nigra pars compacta region of the brain that are enriched in the misfolded presynaptic protein α -synuclein ( α S) and death of the dopaminergic neurons. (hindawi.com)