Regulation of gene ex crispr loci genes bacterial. Medical search. Frequent questions

The technology uses a catalytically dead Cas9 (usually denoted as dCas9) protein that lacks endonuclease activity to regulate genes in an RNA-guided manner. (wikipedia.org)
This is accomplished by introducing point mutations in the two catalytic residues (D10A and H840A) of the gene encoding Cas9. (wikipedia.org)
Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. (mdpi.com)
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. (nature.com)
To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. (nature.com)
We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
Genome editing using clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas9 is an easy and efficient strategy for the generation of gene-modified cells and organisms 1 , 2 . (nature.com)
The CRISPR/Cas9 system consists of two components: Cas9 protein and guide RNA (gRNA). (nature.com)
The natural Cas9 protein possesses a nuclease activity and can induce a DNA double-strand break (DSB) in any genomic sequence guided by a gRNA, provided that a protospacer adjacent motif (PAM) sequence exists in the target locus 3 , 4 , 5 . (nature.com)
Streptococcus pyogenes Cas9 (SpCas9) has been the most widely used Cas9 protein and its PAM sequence is 5′-NGG-3′, which is the only restriction for designing CRISPR/Cas9 target sequences 6 . (nature.com)
Here, we report an easy and efficient construction system for all-in-one CRISPR/Cas9 vectors expressing Cas9 protein and up to seven gRNAs. (nature.com)
In Cas9 nuclease-mediated genome editing, we constructed an all-in-one vector expressing Cas9 nuclease and seven gRNAs and targeted seven genomic loci. (nature.com)
In addition, the plasmids for the second-step reaction retained the structure of a CRISPR/Cas9 expression vector harboring single gRNA and Cas9 expression cassettes and can therefore be used separately for single gene targeting. (nature.com)
Small RNA molecule constituting part of a 3-component naturally occurring CRISPR-Cas defense system in bacteria and serving as a link for associating the crRNA with the Cas9 protein. (corteva.com)
It is called the CRISPR/Cas9 System. (sbmt.org.br)
The CRISPR/Cas9 technology could also contribute to the enhancement of investigations related to genetic modifications in disease-transmitting insect-vectors. (sbmt.org.br)
CRISPR-Cas9 Confers Adaptive Immunity. (sbmt.org.br)
BSTM: Dr. Angela, tell us about your experience with CRISPR/Cas9. (sbmt.org.br)
Two research groups have published the first papers applying the CRISPR/Cas9 system to Leishmania in 2015 (groups from Y. Sterkers and G. Matlashewski, in France and Canada respectively). (sbmt.org.br)
BSTM: How does the CRISPR/Cas9 system work? (sbmt.org.br)
Genome edition by CAS9 enzyme (CRISPR-associated gene 9), an endonuclease then recognizes clustered regularly interspaced short palindromic repeats (CRISPR), is a transforming technology since Cas9 precisely and sequence-specifically cleaves the double DNA strand. (sbmt.org.br)
Three types of endonucleases , namely ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases ), and the CRISPR (clustered regularly interspersed short palindromic regions) associated (Cas9) system have been predominantly utilized for gene editing. (ac.ir)
The emergence of TALENs in 2010 has enabled the genome modification of non- model organisms, while the emergence of the CRISPR/Cas9 system in 2013 as a revolutionary genome-editing tool has allowed us to anticipate the forthcoming new era of genome editing research. (ac.ir)
An example would be the production of edible fungi resistant to browning by inactivation of the genes encoding polyphenol oxidase in 2016 under the non-GMO genetically edited crop plants and production of herbicide-resistant rice and rapeseed using CRISPR/Cas9 systems. (ac.ir)
Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. (elifesciences.org)
In 2015, researchers reported that they had used components of the CRISPR/Cas9 genome editing system to edit genes so that they could propagate in a "Super-Mendelian" fashion. (elifesciences.org)
Recently, new plant breeding technologies (NBPTs) like transcription activator-like effector nucleases, zinc finger nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR Cas9) appeared that are the basis for genetic improvement in soybean. (cornous.com)
An efficient transient assay for CRISPR CAS9 system delivering targeted mutation using synthetic oligo SgRNA in soybean (Glycine max). (cornous.com)
Generation of a multiplex mutagenesis population via pooled CRISPR‐Cas9 in soya bean. (cornous.com)
CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean. (cornous.com)
Thomson Reuters predicts that the developers of the CRISPR/Cas9 genome editing approach may win the Nobel Prize in Chemistry. (pharmaceuticalintelligence.com)
21.1.2 Evaluate your Cas9 gene editing vectors: CRISPR/Cas Mediated Genome Engineering - Is your CRISPR gRNA optimized for your cell lines? (pharmaceuticalintelligence.com)
Crispr/CAS9 strategy is applied to generate Y317F and Y508F mutant knock-in cell clones. (biomedcentral.com)
Single xxt3 and triple xxt3xxt4xxt5 mutant Arabidopsis (Arabidopsis thaliana) plants were generated using CRISPR-Cas9 technology to determine the specific function of XXT3. (bvsalud.org)
We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)

Based on the bacterial genetic immune system - CRISPR (clustered regularly interspaced short palindromic repeats) pathway, the technique provides a complementary approach to RNA interference. (wikipedia.org)
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is one form of gene editing that can be used to precisely modify a gene of interest without otherwise compromising the favorable traits of an elite variety (Rani et al. (ufl.edu)
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. (thebreakthrough.news)
The clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) system provides the majority of bacteria and archaea with adaptive and hereditary immunity against this threat. (pjmonline.org)
2013). However, a unique defense system involving clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) was reported recently. (pjmonline.org)
The discovery and improvement of programmable nucleases (zinc finger-Fokl nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR)) has brought a new era of gene editing. (molcells.org)

Rapid characterization of anti-CRISPR proteins and optogenetically engineered variants using a versatile plasmid interference system. (optobase.org)
Anti-CRISPR (Acr) proteins are encoded by mobile genetic elements to overcome the CRISPR immunity of prokaryotes, displaying promises as controllable tools for modulating CRISPR-based applications. (optobase.org)
However, characterizing novel anti-CRISPR proteins and exploiting Acr-related technologies is a rather long and tedious process. (optobase.org)
Abbreviation for "CRISPR Associated" used to designate proteins that accompany a CRISPR locus that together comprises a CRISPR-Cas defense mechanism. (corteva.com)
It is organized into genes that specify the production of proteins. (corteva.com)
There are several forms of RNA, including messenger RNA (mRNA) which conveys the genetic information from genes (encoded by nucleotides) to proteins (encoded by amino acids). (corteva.com)
In addition, nucleoli are dynamic hubs through which numerous proteins shuttle and contact specific non-rDNA genomic loci. (umassmed.edu)
In the RT-qPCR, expression ratio demonstrated that the α-amylase gene of two different larval stages grown on both proteins treated leaves had significantly differentiated expression and was up-regulated in third instar larvae and down-regulated in fourth instar larvae compared to control. (potatobeetle.org)
Results suggest that the hyper-production of α-amylase in third instar larvae is elicited to compensate for the enzyme activity inhibition at an earlier stage and also down-regulation suggests the existence of a negative feedback of plant proteins on the last instar larvae via impaired food intake and digestive α-amylase activity in Colorado potato beetle. (potatobeetle.org)
Crystal structures are available for archaeal and bacterial NAS-like proteins that carry out simpler aminobutanoyl transferase reactions. (bvsalud.org)
Three of the six up-regulated genes encode potential zinc transporter proteins (ZnuA1, ZnuB1, ZnuC1) suggesting involvement of s479 in regulation of zinc transport. (uni-stuttgart.de)
Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

This sequence is then integrated into the CRISPR genetic locus (2). (sbmt.org.br)
Several spacers from earlier contacts with phages are stored in the CRISPR genetic locus. (sbmt.org.br)
From an analysis of mutants able to grow on glutamate we have identified a genetic locus that when mutated elevates the expression of the GltII glutamate/aspartate transport system. (microbiologyresearch.org)
Data from the analysis of mutants that are impaired in this transport activity are consistent with the presence of the structural gene for the transport system at the same genetic locus. (microbiologyresearch.org)

The amino acid sequence of a protein is encoded by the nucleotide sequence of a corresponding gene. (corteva.com)
Left Panel: After a phage has injected its DNA into the bacterial cytoplasm, a protein complex forms consisting of Cas1 and Cas2. (sbmt.org.br)
Phenotypic analysis of these ' in locus ' mutations based on both expression of Kni protein and adult wing phenotypes, reveals novel unexpected features of L2-CRM function including evidence for a chromosome pairing-dependent process that promotes transcription. (elifesciences.org)
Considering the relevance of insect α-amylases and natural α-amylase inhibitors present in plants to protect against insect damage, we investigated the effect of white bean and rapeseed protein extracts on digestive α-amylase gene expression of the Colorado potato beetle, Leptinotarsa decemlineata (Say). (potatobeetle.org)
The sRNA gene is encoded between a CRISPR RNA locus and the Cas protein gene cluster, the s479 deletion strain is viable and was characterized in detail. (uni-stuttgart.de)
What does this gene/protein do? (cancerindex.org)
Following this, the differentially expressed genes (DEGs) were included in Gene Ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein‑protein interaction network and survival analyses. (cancerindex.org)

Many bacteria and most archaea have an adaptive immune system which incorporates CRISPR RNA (crRNA) and CRISPR-associated (cas) genes. (wikipedia.org)
A piece of DNA comprising nucleotide sequences that are repeated and separated by sequences from invading viruses that serves as part of a naturally occurring CRISPR-Cas defense system in bacteria to fight invading viruses. (corteva.com)
A Small RNA molecule encoded by the CRISPR locus constituting part of a 3-component naturally occurring CRISPR-Cas defense system in bacteria. (corteva.com)
The genomic component of the CRISPR-Cas system is formed by a series of variable spacers, which in some cases share sequence similarity with viruses, plasmids, or bacteria. (pjmonline.org)

Targeting specificity is determined by complementary base-pairing of a single guide RNA (sgRNA) to the genomic locus. (wikipedia.org)
All targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease . (ac.ir)

The difference between CRISPRi and RNAi, though, is that CRISPRi regulates gene expression primarily on the transcriptional level, while RNAi controls genes on the mRNA level. (wikipedia.org)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

Together, sgRNA and dCas9 constitute a minimal system for gene-specific regulation. (wikipedia.org)
For dCas9 (based on a Type-2 CRISPR system), repression is stronger when the guide RNA is complementary to the non-template strand. (wikipedia.org)
In the human pathogen Streptococcus mutans, the canonical peptide-based quorum-sensing system is an inducible DNA repair system that is pivotal for bacterial survival. (bvsalud.org)
RNA sequencing revealed that triggered persistence was associated with the upregulation of genes related to several stress defense mechanisms, notably, multidrug efflux pumps, the arginine deaminase pathway, and the Opu/Opc system. (bvsalud.org)
Here, we established a versatile plasmid interference with CRISPR interference (PICI) system in Escherichia coli for rapidly characterizing Acrs and developing Acr-based technologies. (optobase.org)
Our system provides an efficient targeting strategy for multiplex genome/epigenome editing, simultaneous activation/repression of multiple genes and beyond. (nature.com)
Adaptation of the bacterial CRISPR-Cas system for genome editing purposes by combining crRNA and tracrRNA into a single guide RNA molecule. (corteva.com)
This system is present in most archaeal species and approxi-mately half of all bacterial species. (pjmonline.org)
A unique feature of the CRISPR-Cas system is that it provides adaptive and hereditary immunity against foreign nucleic acids, mim-icking in some ways the immune systems of eukaryotes. (pjmonline.org)
The CRISPR-Cas system comprises genomic (CRISPR) and proteomic (Cas) components. (pjmonline.org)
The system represents a great ease in gene edition processes in various organisms and their outcomes are important for pure knowledge, basic science and can also open new horizons to applied science. (sbmt.org.br)
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. (cornous.com)
Here the biofilm formation is not influenced by the competence system, despite gene expression analysis of the competence genes appears to be approximately the same in all models. (dub-signal.com)
However, there are fundamental differences between our heterologous system and natural CRISPR-Cas systems. (billfryer.com)
Together this might suggest that the CRISPR-Cas system is involved in s479 function. (uni-stuttgart.de)
In particular, the use of the CRISPR system enabled convenient programming and manipulation of the genome at specific target loci. (molcells.org)

CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. (wikipedia.org)
The CRISPR interference (CRISPRi) technique was first reported by Lei S. Qi and researchers at the University of California at San Francisco in early 2013. (wikipedia.org)

The geno-mic component is a DNA loci containing short frag-ments of targeted nucleic acid sequences (spacers) inter-spaced by short repeated sequences (repeats). (pjmonline.org)
These regions are interspaced with repeat sequences that are identical or almost identical within a single CRISPR cassette. (pjmonline.org)

Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis. (cdc.gov)

Using the knowledge acquired, we build a synthetic circuit that allows obtaining two gene expression programs depending solely on TF dynamics. (optobase.org)
These results help elucidate how gene expression is regulated in mammalian cells and open up the possibility to build complex synthetic circuits steered by TF dynamics. (optobase.org)

We found that the tricarboxylic acid cycle-associated enzyme α-ketoglutarate (α-KG) dehydrogenase (KGDH) entered the nucleus, where it interacted with various JMJs to regulate α-KG-dependent histone demethylations by JMJs, and thus controlled genome-wide gene expression in plants. (bvsalud.org)

The researcher supplied the necessary molecular constructions, and still in 2017, we started our first gene knockout experiments in different Leishmania species. (sbmt.org.br)

RNA is essential in various biological roles in coding, decoding, regulation, and expression of genes. (corteva.com)
Growth of Escherichia coli on glutamate as sole carbon source only occurs in strains carrying mutations that increase the expression of genes encoding glutamate transport systems. (microbiologyresearch.org)

It was she, along with colleague Emmanuelle Charpentier, who made the breakthrough with CRISPR - with the pair winning the 2020 Nobel Prize for Chemistry for their efforts. (thebreakthrough.news)

Depending on the nature of the CRISPR effector, either the template or non-template strand leads to stronger repression. (wikipedia.org)

These NBPTs proved beneficial in the improvement of soybean through precision genome engineering and gene functional characterization. (cornous.com)

Moreover, transient gene expression through viral vectors allowed the accumulation of carotenoids outside the plastid. (frontiersin.org)

In response to different stimuli many transcription factors (TFs) display different activation dynamics that trigger the expression of specific sets of target genes, suggesting that promoters have a way to decode dynamics. (optobase.org)

Assemblages of multiple genes are organized into chromosomes. (corteva.com)

Roles of the trkB and trkC gene products of Escherichia coli in K + transport. (microbiologyresearch.org)
Kempsell K.E. 1987 The regulation of glutamate and melibioseutilisation in Escherichia coli. (microbiologyresearch.org)
Genetic studies of the phs locus in Escherichia coli, a mutation causing pleiotropic lesions in metabolism and pH homeostasis. (microbiologyresearch.org)

By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)

Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)

The knirps ( kni ) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila . (elifesciences.org)
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. (cancerindex.org)

Plasmid insertion mutagenesis and lac gene fusion with mini-Mu bacteriophage transposons. (microbiologyresearch.org)

New metabolic engineering approaches have recently been developed to modulate carotenoid content, including the employment of CRISPR technologies for single-base editing and the integration of exogenous genes into specific "safe harbors" in the genome. (frontiersin.org)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)

These methods broadened the targeting range, thus allowing modification of numerous genes for phenotypic screens. (molcells.org)

Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
CRISPR (pronounced 'crisper') is a revolutionary gene-editing tool that allows scientists to make precise changes to the DNA sequence of living organisms - including human cells. (thebreakthrough.news)
These include providing new ways to explore how genes control the formation and activity of different organisms. (elifesciences.org)

This approach is known as 'CRISPR' gene editing. (ufl.edu)
With gene editing, the final product can match that obtained by conventional plant breeding, but in a much shorter timeframe. (ufl.edu)
We anticipate that, over the next decade, CRISPR and other gene editing techniques will be used to rapidly develop elite strawberry varieties with improved disease resistance, fruit quality, and other valuable attributes. (ufl.edu)
What is CRISPR gene editing? (ufl.edu)
Gene editing technology can be used to almost surgically adjust the gene or genes behind that deleterious trait. (ufl.edu)
For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
Does CRISPR gene editing = transgenic technology? (ufl.edu)
Early indications suggest that gene editing should be regulated like conventionally bred crops, as the final product can simply match what may be done by conventional breeding. (ufl.edu)
We published another article about CRISPR gene editing in crop improvement ( https://edis.ifas.ufl.edu/publication/HS1334 ). (ufl.edu)
Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind's ability to deliver precise genotypes in crops. (frontiersin.org)
This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)
These methods are advancing rapidly and already enabling crop scientists to make use of the precision of CRISPR gene editing tools. (frontiersin.org)
Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)
Such has been its success and potential for genome editing so significant, its use has been swiftly adopted by many offering a broad range of opportunities for CRISPR investing. (thebreakthrough.news)
By using the CRISPR gene editing technology, it was able to produce the fruit with high levels of Gamma-AminoButyric Acid (GABA), an amino acid said to aid relaxation and help lower blood pressure. (thebreakthrough.news)
There is no existence of internationally agreed regulatory framework for gene editing products and countries are in the process of evaluating whether and to what extent current regulations are adequate for…products related to gene editing. (thebreakthrough.news)
Its researchers are working to harness CRISPR-based genome editing technologies for human therapeutic use. (thebreakthrough.news)
Targeted genome engineering or editing enables researchers to modify genomic loci of interest in a precise manner, which has a turning point in medicine, biological research, and biotechnology. (ac.ir)
Treatment of human immunodeficiency virus (HIV) infection with ZFN-mediated CCR5 gene disruption is one of the indicator examples of the ability of ZFNs in genome editing. (ac.ir)
including the two researchers involved in the 2015 work - have used a new active genetic element called a CopyCat element and more traditional genome editing to analyze the control of a gene that coordinates the formation of a simple structure in a fruit fly - a vein in the wing. (elifesciences.org)
used genome editing to make mutations in a stretch of DNA that regulates the gene involved in wing vein formation. (elifesciences.org)
21.1.4 Prediction of the Winner RNA Technology, the FRONTIER of SCIENCE on RNA Biology, Cancer and Therapeutics & The Start Up Landscape in BostonGene Editing - New Technology The Missing link for Gene Therapy? (pharmaceuticalintelligence.com)
However, several problems remain and hinder efficient and specific editing of target genomic loci. (molcells.org)
For instance, effective transfer of programmable nucleases to living cells, tissues, or organs that contain the target gene is important for genome editing. (molcells.org)

In this minireview, we describe recent efforts made to overcome these problems by using target-specific endonucleases, including CRISPR. (molcells.org)

Subsequent repair of this DSB by non-homologous end joining (NHEJ) or homology-directed repair (HDR) generates the desired genetic modifications such as gene disruption, gene insertion, gene correction, etc. (ac.ir)

Epigenetic regulation is necessary for the production of differentiated cells throughout plant development, as well as maintaining the stability and integrity of the gene expression profiles. (ac.ir)
Epigenetic Regulation in Plant Responses to the Environment. (ac.ir)
This entry briefly introduces the different stages of T cell differentiation and will discuss recent findings on the epigenetic regulation of this process. (encyclopedia.pub)
Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)

Specific genes that can be activated by inducers are called inducible genes. (dub-signal.com)

The UF/IFAS strawberry breeding program has identified several important gene regions controlling disease resistance traits that are directly relevant to Florida growers. (ufl.edu)
We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)

This enables us to identify candidate genes underlying glucosinolate diversity, trichome density, and study the genetics of biochemical variation for glucosinolate and saponins. (pacb.com)
CRISPR technology enables scientists to target and modify specific genes within an organism's DNA, similar to using a pair of molecular scissors. (thebreakthrough.news)
This mechanism enables differentiated cells in a multicellular organism to express only the genes which are necessary for their own activity. (ac.ir)

Sequence-specific activation of gene expression refers to CRISPR activation (CRISPRa). (wikipedia.org)
CRISPR, on the other hand, can be used to precisely change DNA sequence, switching it from one naturally occurring variant to another naturally occurring variant. (ufl.edu)

The Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants by maintaining homeostasis of water, salt ions and ROS. (cornous.com)
In this review, our goal is to introduce epigenetics and its different applications in plants, especially in production of transgenic plants, plants tolerate to biotic and abiotic stresses and understanding the mechanisms of gene silencing. (ac.ir)

Since then we have succeeded generating knockout for several genes of Leishmania major and Leishmania braziliensis . (sbmt.org.br)

A transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary-phase cells and the persisters. (bvsalud.org)

The aim of this review is to summarize the current status of CRISPR-Cas research, including the mechanisms of action, the classification of different types and subtypes of these systems, and the development of new CRISPR-Cas-based molecular biology tools. (pjmonline.org)

Identification of the transcription start site of a target gene and considering the preferences of sgRNA improves efficiency, as does the presence of accessible chromatin at the target site. (wikipedia.org)

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. (thermofisher.com)

Typical protocols to make gene edited (GE) varieties also use transgenes, even though these may be undesirable in the final crop product. (frontiersin.org)

Anatomy3

Organisms33

Diseases2

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes59

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care2

Geographicals1

Cas937

Regularly interspaced6

Proteins13

Genetic locus4

Protein7

Bacteria4

Genomic locus2

Transcriptional2

System16

Interference2

Sequences2

RRNA genes1

Synthetic2

Genome-wide1

Species1

Expression of ge2

20201

Effector1

Characterization1

Vectors1

Target genes1

Multiple genes1

Escherichia3

Variants1

Upregulation1

Encodes2

Plasmid1

Modulate2

Phenotypic1

Organisms3

Editing24

Endonucleases1

Insertion1

Epigenetic4

Inducible1

Resistance2

Enables3

Sequence2

Transgenic2

Leishmania1

Differential gene expression1

Mechanisms1

SgRNA1

Viral1

Crop1