• PT181
  • This mode of regulation is essentially the same as that of the countertranscript-driven attenuators that control copy number in pT181, pAMbeta1 and pIP501 and related Staphylococcal plasmids. (wikipedia.org)
  • strains
  • Plasmid cured strains, however, do not show this dependency on Ca 2+ and are thus, Ca 2+ independent (CI). (springer.com)
  • The IncP-1 plasmid group (IncP plasmids in Escherichia coli) of which RK2 is a part has been described as "highly potent, self-transmissible, selfish DNA molecules with a complicated regulatory circuit" RK 2 was first isolated in connection with an outbreak of antibotic-resistant Pseudomonas aeruginosa and Klebsiella aerogenes in Birmingham in 1969, as one of a family of plasmids implicated in transfer of Ampicillin resistance between bacterial strains. (wikipedia.org)
  • Malgorzata Adamczyk and Grazyna Jagura-Burdzy: "Spread and survival of promiscuous IncP-1 plasmids", Acta Biochimica Polonica, Vol 50, no. 2/2003, p. 425-453 LEWIS C. INGRAM, M. H. RICHMOND, AND R. B. SYKES: "Molecular Characterization of the R Factors Implicated in the Carbenicillin Resistance of a Sequence of Pseudomonas aeruginosa Strains Isolated from Burns", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 1973, p. 279-288 Thomas CM et al. (wikipedia.org)
  • bacteria
  • In this study, we determined the distributions of the well-known NAPs Fis, H-NS, HU, IHF, and Lrp and the newly found NAPs MvaT and NdpA among the whole-sequenced 1382 plasmids found in Gram-negative bacteria. (hindawi.com)
  • A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. (wikipedia.org)
  • coli
  • In E. coli, multiple plasmid copies appear to cluster together, creating a few multiplasmid clusters in each cell. (wikipedia.org)
  • Two such mutants, PFF1cop254D and PFF1cop271C, increase the copy number of PFF1 in E. coli from approximately 39-40 to about 501 and 113 plasmids per cell, respectively. (wikipedia.org)
  • pBR322
  • For example, pBR322 is a low copy number plasmid (~20 copies/cell) from which several very high copy number cloning vectors (~1000 copies/cell) have been derived. (wikipedia.org)
  • Fragment
  • When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. (addgene.org)
  • sequences
  • Repeated DNA Sequences Recombine 1,000 Times More Frequently in a Plasmid Than in the Chromosome of BacillusSubtilis. (moluna.de)
  • genetic elements
  • Plasmid publishes original research on genetic elements in all kingdoms of life with emphasis on maintenance, transmission and evolution of extrachromosomal elements . (elsevier.com)
  • transfer
  • Plasmid welcomes topics such as horizontal gene transfer, including antibiotic resistance transfer, and molecular aspects of microbial ecology. (elsevier.com)
  • It should be noted that plasmids with the NAP gene homolog also carried the relaxase gene involved in the conjugative transfer of plasmids more frequently than did those without the NAP gene homolog, implying that plasmid-encoded NAP homologs positively contribute to transmissible plasmids. (hindawi.com)
  • cell
  • 1)development of cell plating methods for the methanosarcina, 2) screening and isolation of plasmids from the acetogenic methanogens, and 3) construction of gene libraries for M. acetivorans. (dtic.mil)
  • studies
  • These studies should provide a firm basis for understanding the regulation of acetate utilization in the methanogen, Methanosarcina acitivorans. (dtic.mil)