Regulation loci genes bacterial. Medical search. Frequent questions

An infectious transfer and expression system for genomic DNA loci in human and mouse cells. (ox.ac.uk)
To demonstrate that genomic loci transferred in this way are functional, the complete human hypoxanthine phosphoribosyltransferase (HPRT) locus contained within a 115-kilobase BAC insert was shown to be expressed when delivered by infection into both a human HPRT-deficient fibroblast cell line and a mouse primary hepatocyte culture derived from Hprt-/- mice. (ox.ac.uk)
The advantage of CRISPR is that its specificity depends largely on a guide RNA (gRNA) that can be readily programmed to target different genomic loci. (nature.com)
Recent research has uncovered a large number of genomic loci encoding long or small RNAs with low protein-coding potential. (biomedcentral.com)
26 genomic loci were identified whose haploinsufficiency can modify these CDK8- or CycC-specific phenotypes. (sdbonline.org)

Other genes were found to have a role in the pathogenesis of the disease through defects in sensing and processing of signals that are related to regulation of innate and adaptive immunity. (medscape.com)
I also have a strong background in bacterial pathogenesis of both Animal and Plant Hosts. (ncl.ac.uk)
Genome-wide association studies and subsequent meta-analyses of these two diseases as separate phenotypes have implicated previously unsuspected mechanisms, such as autophagy, in their pathogenesis and showed that some IBD loci are shared with other inflammatory diseases. (bioseek.eu)
Although a number of bacteria and viruses have been show to influence serotonin signaling, no study has investigated the effect that serotonin has on bacterial pathogenesis. (grantome.com)

We demonstrate that the vast majority of differentially regulated enhancers on acute stimulation are subject to tolerance and that expression quantitative trait loci, disease-risk variants and eRNAs are enriched in these regulatory regions and related to context-specific gene expression. (ox.ac.uk)

Type I toxin-antitoxin (TA) systems are widespread genetic modules in bacterial genomes. (mdpi.com)
My laboratory uses, bacterial genetics, protein biochemistry, structural biology, bioinformatics and microscopic techniques to validate our genetic findings. (ncl.ac.uk)
For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)
Botstein is honored for contributions that include developing methods for defining genetic pathways, mapping genomes, and analyzing gene expression. (genestogenomes.org)
We show enrichment in differential enhancers for tolerance involving transcription factors NFκB-p65, STATs and IRFs and prioritize putative causal genes directly linking genetic variants and disease risk enhancers. (ox.ac.uk)
We further delineate similarities and differences in epigenetic landscape between stem cell-derived macrophages and primary cells and characterize the context-specific enhancer activities for key innate immune response genes KLF4, SLAMF1 and IL2RA.ConclusionsOur study demonstrates the importance of context-specific macrophage enhancers in gene regulation and utility for interpreting disease associations, providing a roadmap to link genetic variants with molecular and cellular functions. (ox.ac.uk)
In a genetic screen for suppressors of Arabidopsis (Arabidopsis thaliana) accelerated cell death 6-1 (acd6-1), a small constitutive defense mutant whose defense level is grossly in a reverse proportion to plant size, we identified an allele of the canonical flowering regulatory gene FLOWERING LOCUS K HOMOLOGY DOMAIN (FLK) encoding a putative protein with triple K homology (KH) repeats. (nsf.gov)
Mutant analysis revealed complex genetic interactions between FLK and several major SA pathway genes. (nsf.gov)
Functional characterization of the identified regions found relationships between methylation at these loci and nearby genetic variation contributing to chronic painful TMD and with gene expression of proximal genes. (iasp-pain.org)
The substance does not meet the criteria for classification for genetic toxicity according to or Regulation No.1272/2008/EC or Directive 67/548/EEC. (europa.eu)
To uncover the genetic basis for lower immobility time, we first performed quantitative trait locus (QTL) mapping using CS and C57BL/6J mice, which revealed significant QTLs on chromosomes (Chrs) 4 (FST) and 5 (TST and FST). (fujita-hu.ac.jp)
With the advances in BMT and gene therapy, patients now have a better likelihood of developing a functional immune system in a previously lethal genetic disease. (medscape.com)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

We also observe considerable overlap between susceptibility loci for IBD and mycobacterial infection. (bioseek.eu)
Lupus susceptibility loci promote anti-bacterial host defenses. (uh.edu)
Susceptibility loci for lupus: a guiding light from murine models? (jci.org)

The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation. (elifesciences.org)
Linkage and comparative mapping studies indicate that the transferred chromosome segment contains a number of candidate genes for hypertension, including genes encoding a brain dopamine receptor and a renal epithelial potassium channel. (jci.org)
These findings demonstrate that BP regulatory gene(s) exist within the differential chromosome segment trapped in the SHR-Lx congenic strain and that this region of chromosome 8 plays a major role in the hypertension of SHR vs. BN-Lx rats. (jci.org)
Notably, mutations in the TPP1 OB-fold domain compromise telomerase-dependent telomere extension but not telomere end protection [ 18 , 21 ], indicating that TPP1 performs a role in telomerase regulation which is distinct from its contribution to chromosome end protection. (prolekarniky.cz)
Lateral gene transfers resulted in different linkages between the ospC gene and loci of the chromosome or other plasmids. (cdc.gov)

GBS production demonstrate differences in GBS isolates of the same clonal of -h/c is encoded by the genes of the cyl operon ( 5 , 6 ) origin but varying phenotypes. (cdc.gov)

The bacterially produced tryptophan derivative indole is known to influence intestinal barrier function, and has also been shown to regulate virulence gene expression in EHEC. (grantome.com)
Regulation of bacterial virulence gene expression by the host environment. (jci.org)

It induces both local effects in the gut, as well as alterations in distant organs through stimuli generated by bacteria, structural bacterial components, and microbial metabolites [ 7 ]. (biomedcentral.com)
He started working with the organism as a Yale graduate student spurred by the recent discovery in bacteria of operons-clusters of genes with related function controlled by a shared regulatory element transcribed into a single messenger RNA. (genestogenomes.org)
Upon detection of environmental alterations, bacteria adjust their gene expression in order to obtain a phenotype that is suitable for the environment to which they are exposed ( Federle, 2012 ). (frontiersin.org)
This study also identified 43 growth-impairing inserts carrying multiple loci targeting 105 genes, of which 25 have homologs reported as essential in other bacteria. (biomedcentral.com)

Book Chapter in Genes, Genomes and Genomics. (uh.edu)
The population of P. aeruginosa in the Philippines was diverse, with clonal expansions of XDR genomes belonging to multi-locus sequence types ST235, ST244, ST309, and ST773. (who.int)

A significant number of bacterial species encode multiple copies of the filament building block - flagellin. (ncl.ac.uk)
We have studied how bacterial species such as the pathogen Salmonella enterica maintain a discrete number of flagellar per cell during cell growth and division. (ncl.ac.uk)
We complement our study on flagellar abundance with research to understanding how a filament is assembled from multiple flagellins, a trait maintained by many bacterial species. (ncl.ac.uk)
and 16 were "novel" essential genes with no homologs reported to have an essential role in other bacterial species. (biomedcentral.com)

Moreover, major phenotypic changes have been reported in several bacterial strains with a deregulated methyltransferase, but the corresponding methylome has rarely been described. (pacb.com)
Building on excellent preliminary studies expressing the Campylobacter jejuni N-linked pgl glycan, we will combine novel synthetic biology approaches to develop a new platform technology for optimal glycan expression using (i) 'refactoring' of glycan clusters (deconstructing and rebuilding in a tunable modular format) and combinatorial optimisation, (ii) engineered regulation, and (iii) our bank of rationally designed E. coli host strains. (ukri.org)
There were no significant increases in the frequency of revertant colonies recorded for any of the bacterial strains, with any dose of the test item, either with or without metabolic activation (S9-mix) and the test item was considered to be non-mutagenic (OECD 471, EU Method B.13/14, OPPTS 870.5100 and relevant Japanese guidelines). (europa.eu)
Sub-objective 4.1: Analysis of ExPEC isolated from chickens and humans: biofilm assays, virulence gene profiles, antimicrobial resistance profiles, whole genome comparison of ExPEC strains isolated from chicken and human infections. (usda.gov)
We will use omic technologies to analyze a large variety of strains of each of the pathogens to identify genes and proteins necessary for pathogens to survive stresses encountered in food environments and cause human illness. (usda.gov)
dimers, oligomeric reaction products with tall-oil fatty acids and triethylenetetramine in bacterial cells in vitro (McGarry, 2012), negative results were obtained in Salmonella typhimurium strains TA98, TA100, TA102, TA1535 and TA1537, in the presence and in the absence of metabolic activation (S9 mix). (europa.eu)
To identify the quantitative trait gene on Chr 5, we narrowed the QTL interval to 0.5 Mb using several congenic and subcongenic strains. (fujita-hu.ac.jp)
In addition, we sequenced 99 ospC flanking sequences from different lineages and compared the complete cp26 sequences of 11 strains as well as the cp26 bbb02 loci of 56 samples. (cdc.gov)
Over time, OspC genes were transferred between strains through recombinations involving the whole or parts of the gene and one or both flanks. (cdc.gov)

In addition, Esr41 activated the expression of flagellar Class 3 genes by indirectly inducing the transcription of fliA, which encodes the sigma factor for flagellar synthesis. (nih.gov)

We identified 30 loci that predict tolerance, many of which are in genes involved in the regulation of immunity and metabolism. (datadryad.org)
Fink's work in the budding yeast Saccharomyces cerevisiae led to our deep understanding of gene expression control in eukaryotic metabolism. (genestogenomes.org)
Most DEGs were involved in plant hormone signal transduction, plant-pathogen interaction and phenylalanine metabolism, and the genes significantly up-regulated in PI 114490 at 6 DPI were associated with defense response pathways. (frontiersin.org)
When differentiated human THP-1 cells were infected with MAP, the expression of various genes associated with stress responses and metabolism was altered in both host and MAP at 3 h post-infection. (biomedcentral.com)
Notably, alterations in gene sets involved in arginine metabolism were observed in both the host and MAP, along with significant downregulation of NOS2 expression. (biomedcentral.com)
Extensive regulation by σS of genes involved in metabolism and membrane composition, and down-regulation of the respiratory chain functions, were important features of the σS effects on gene transcription that might confer fitness advantages to bacterial cells and/or populations under starving conditions. (hal.science)

Recently, In the traditional clinical view of invasive GBS patho- cases of the severe, life-threatening syndromes of necrotiz- genesis, a bacterial isolate enters a normally sterile site from ing fasciitis ( 1 ) and toxic shock syndrome due to GBS have a focus of mucosal colonization or recent acquisition of the been reported in neonates ( 2 ) and immunocompromised pathogen. (cdc.gov)
In addition to late flowering, the flk mutants exhibited decreased resistance to the bacterial pathogen Pseudomonas syringae and increased resistance to the necrotrophic fungal pathogen Botrytis cinerea. (nsf.gov)
Thus, this study provides mechanistic support for flk suppression of acd6-1 and establishes that FLK is a multifunctional gene involved in regulating pathogen defense and development of plants. (nsf.gov)
Borrelia burgdorferi , an emerging bacterial pathogen, is maintained in nature by transmission from one vertebrate host to another by ticks. (cdc.gov)
By means of DNA sequencing of a large sample collection of the pathogen from across the United States, we studied the gene for the bacterium's highly diverse OspC protein, protective immunity against which develops in animals. (cdc.gov)

When LEE expression is activated, flagellar gene expression is down-regulated because bacterial flagella induce the immune responses of host cells at the infection stage. (nih.gov)
Therefore, this inverse regulation is also important for EHEC infection. (nih.gov)
Urinary tract infections (UTIs) are a common bacterial infection with symptoms that include urinary frequency, urgency to void, dysuria and abdominal pain. (ncl.ac.uk)
At present it is assumed that the relationship between an individual's susceptibility and bacterial virulence determines the balance between tolerance of invading pathogens and the mounting of an immune response, which in turn dictates the course of infection and subsequent recurrence. (ncl.ac.uk)
We find enrichment for context-specific eQTL involving endotoxin response and specific infections and delineate specific differential regions informative for GWAS variants in inflammatory bowel disease and multiple sclerosis, together with a context-specific enhancer involving a bacterial infection eQTL for KLF4. (ox.ac.uk)

Regulatory genes which encode a cyclic AMP receptor protein required for L-arabinose utilization in E. coli. (nih.gov)
This is because they invariably consist of multi-gene loci which encode several enzymes that synthesise complex glycan structures. (ukri.org)

Discussion Differentially expressed genes inC. jejuniNCTC 11168 and itsluxSmutant InVibriospp, AI-2 functions as an extracellular signalling molecule. (hsdpathway.com)
Overall, the numbers of differentially expressed genes (DEGs) were higher in the resistant tomato line PI 114490 than in the susceptible line OH 88119, and the numbers of DEGs were higher at 6 DPI than at 6 HPI. (frontiersin.org)
A majority of loci were similarly differentially methylated in acute TMD consistent with observed transience or persistence of symptoms at follow-up. (iasp-pain.org)

We demonstrate the utility of our new approach by applying it to identify genes used by Escherichia coli to survive in a wide range of different concentrations of the biocide Triclosan. (biorxiv.org)

dimers, oligomeric reaction products with tall-oil fatty acids and triethylenetetramine in a bacterial reverse mutation assay (Ames test). (europa.eu)

DNA methylation can serve to control diverse phenomena in eukaryotes and prokaryotes, including gene regulation leading to cell differentiation. (pacb.com)
The rare unmethylated GATC motifs were preferentially located in putative promoter regions, and most of them were methylated after Dam methyltransferase overexpression, suggesting that DNA methylation is involved in gene regulation. (pacb.com)
Our findings bring key insight into bacterial methylomes and encourage further research to decipher the role of loci protected from DNA methylation in gene regulation. (pacb.com)
These findings reveal epigenetic contributions to chronic painful TMD through methylation of the genes FMOD, PM20D1, ZNF718, ZFP57, and RNF39, following the development of acute painful TMD. (iasp-pain.org)

We report here that a small regulatory RNA (sRNA), Esr41, mediates LEE repression and flagellar gene activation. (nih.gov)
Here, we systematically delineate the acute innate immune response to endotoxin in terms of human macrophage enhancer activity and contrast with endotoxin tolerance, profiling the coding and non-coding transcriptome, chromatin accessibility and epigenetic modifications.ResultsWe describe the spectrum of enhancers under acute and tolerance conditions and the regulatory networks between these enhancers and biological processes including gene expression, splicing regulation, transcription factor binding and enhancer RNA signatures. (ox.ac.uk)
Identification and characterization of the host resistance as well as regulatory genes can provide insights into understanding the mechanisms of host resistance and the use of host resistance to develop new cultivars with durable resistance. (frontiersin.org)
a transcriptional cis regulatory region that when located between an enhancer and a gene's promoter prevents the enhancer from modulating the expression of the gene. (insdc.org)
a regulatory region that controls epigenetic imprinting and affects the expression of target genes in an allele- or parent-of-origin-specific manner. (insdc.org)

We use in vivo and in vitro models to determine the biological function of uncharacterized candidate genes for bone mineral density, bone formation, and bone strength. (cuanschutz.edu)
When Fink's career began, the discovery of DNA and the molecular biology revolution had changed little about the study of yeast genetics because there was no method to introduce DNA into a yeast cell, a critical step for tinkering with genes in vivo . (genestogenomes.org)
Furthermore, this analyses confirmed the positive roles of CDK9 and Yorkie in regulating Mad-dependent gene expression in vivo. (sdbonline.org)

During my career I have gained valuable experience working with a range of bacterial pathogens. (ncl.ac.uk)
Targeting of novel essential pathways is expected to play an important role in the discovery of new antibacterial agents against bacterial pathogens, such as Pseudomonas aeruginosa , that are difficult to control because of their ability to develop resistance, often multiple, to all current classes of clinical antibiotics. (biomedcentral.com)

The locus of enterocyte effacement (LEE) in EHEC encodes a type three secretion system and effector proteins, all of which are essential for bacterial adherence to host cells. (nih.gov)
In eukaryotic cells, chromatin comprises a complex consisting of DNA, RNA, and proteins where covalent modifications correlate with regulation of gene expression [ 1 ]. (biomedcentral.com)
They cloned the HIS4 locus, yeast's best candidate for an operon and showed that it in fact encoded a single multifunctional protein, rather than multiple proteins under common regulation. (genestogenomes.org)
Increasing evidence indicates the importance of posttranscriptional regulation of plant defense by RNA binding proteins. (nsf.gov)
Downstream genes involved in type VII secretion systems, cell wall synthesis (polyketide biosynthesis proteins), and iron uptake were changed in response to the intracellular environment of macrophages. (biomedcentral.com)
The HLA-DQB1 gene has many different normal variations, allowing each person's immune system to react to a wide range of foreign proteins. (medlineplus.gov)

The flagellum is a unique molecular machine driving bacterial movement through liquid environments. (ncl.ac.uk)
Molecular and in silico studies of such non-coding RNAs (ncRNAs) have expanded our understanding of gene transcriptional regulation and the generation of organism complexity. (biomedcentral.com)
Drosophila has been a powerful system for deciphering the conserved mechanisms that regulate hormone signaling, sugar and lipid homeostasis, and the molecular mechanisms underlying the nutritional regulation of development. (sdbonline.org)
This study also provides a firm basis for future studies to address molecular mechanisms of indirect regulation of gene expression by σS. (hal.science)

Translational gene-reporter fusion experiments revealed that Esr41 regulates ler expression at a post-transcriptional level, and pch transcription, probably via an unknown target of Esr41. (nih.gov)
Fink and his students showed that many genes related to amino acid biosynthesis were controlled by a single master regulator-the transcription factor Gcn4p. (genestogenomes.org)
2) sequence segment located between the promoter and the first structural gene that causes partial termination of transcription. (insdc.org)

Furthermore, AtRAD54, which performs an important role in the homologous recombination repair pathway, was involved in the pairing of homologous loci with γ-irradiation. (nature.com)
We found that AtRAD54, which performs an important role in the homologous recombination (HR) repair pathway, was involved in the approach of two homologous loci under γ-irradiation. (nature.com)
Our results suggest that the transient reduction in inter-allelic distance and increase in pairing frequency of homologous loci after DSB result in partial chromatin reorganisation of interphase nuclei and that AtRAD54 contributes to the subcellular movement of homologous loci in the HR repair pathway. (nature.com)

Ge- analysis of these variants provides insight into the potential netic analysis found a 3-bp deletion in the covR gene of contribution of specifi c bacterial virulence factors to these the HH/LC variant. (cdc.gov)

The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated adenines. (pacb.com)

We used RNAi to confirm that a subset of mapped genes have a role in defence, including putative wound repair genes grainy head and debris buster. (datadryad.org)

My expertise utilise bacterial genetics to study fundamental aspects of the bacterial life cycle and host-microbe interactions. (ncl.ac.uk)
We hypothesize that by focusing on a cellular phenotype and employing systems genetics methodologies, we will have increased success in discovering genes that specifically modulate bone formation, as compared to traditional mapping studies of higher-level phenotypes. (cuanschutz.edu)

Our lab is interested in identifying genes that participate in the regulation of bone mechanical integrity and bone mass but do so via interactions with environmental modulators. (cuanschutz.edu)
Among soil microorganisms, arbuscular mycorrhizal (AM) fungi and bacterial Rhizobium can promote plant growth as well as control fungal diseases [ 2 ], suggesting that there is an interconnection of signaling pathways mediating root symbiotic and pathogenic interactions, and miRNAs are implicated in these biotic relationships. (biomedcentral.com)
in addition, their comprehensive miRNA profiling experiments enable them to further decipher miRNA-based regulation modules in response to specific biotic interactions. (biomedcentral.com)

These results are consistent with a previous model that miRNAs might have been derived from inverted-duplication of targeted protein-coding genes [ 7 ]. (biomedcentral.com)
One of the few antigens against which mammals develop protective immunity is the highly polymorphic OspC protein, encoded by the ospC gene on the cp26 plasmid. (cdc.gov)
The most well-studied of these genes is HLA-DQB1 , which provides instructions for making part of a protein that plays an important role in the immune system. (medlineplus.gov)

Fink continued to pursue the question of how eukaryotes regulate their genes, as well as many others that piqued his interest. (genestogenomes.org)

Transcriptional regulators enable the microbes to sense and respond to environmental stimuli, by altering gene expression. (frontiersin.org)
MAP upregulates stress-responsive global gene regulators, such as two-component systems and sigma factors, in response to oxidative and cell wall stress. (biomedcentral.com)

100 kilobases, a technique that may prove useful for analyzing gene expression from the human genome. (ox.ac.uk)

Bacterial artificial chromosomes (BACs) and P1-based artificial chromosomes (PACs) have proved excellent tools for the human genome sequencing projects. (ox.ac.uk)
All these phenotypes were rescued in transgenic mice with bacterial artificial chromosomes containing wild-type Usp46. (fujita-hu.ac.jp)

Sequence analysis of the gelE flanking regions revealed three genes (fsrA, fsrB and fsrC) upstream of gelE that show homology to the genes in a locus (agr) that globally regulates the expression of virulence factors in Staphylococcus aureus and one open reading frame (sprE) with homology to bacterial serine protease downstream of gelE. (uth.edu)

We aimed to identify novel essential genes in P. aeruginosa by shotgun antisense screening. (biomedcentral.com)
To also target low expressed essential genes, we included some variant steps that were expected to overcome the non-stringent regulation of the promoter carried by the expression vector used for the shotgun antisense libraries. (biomedcentral.com)
For the first time in P. aeruginosa , we applied regulated antisense RNA expression and showed the feasibility of this technology for the identification of novel essential genes. (biomedcentral.com)
The discovery of novel essential genes or pathways that have not yet been targeted by clinical antibiotics can underlie the development of alternative effective antibacterials to overcome existing mechanisms of resistance. (biomedcentral.com)
A recent refinement of this approach allows the roles of essential genes in conditional stress survival to be inferred by altering their expression. (biorxiv.org)

AlbaTraDIS automatically identified all well characterised Triclosan resistance genes, including the primary target, fabI . (biorxiv.org)
This was further supported by long-read sequencing of one representative XDR isolate, which revealed the presence of an integron carrying multiple resistance genes, including bla VIM-2 , with differences in gene composition and synteny to other P. aeruginosa class 1 integrons described before. (who.int)

Our in-depth knowledge of flagellar assembly and its regulation allow us to investigate the interaction of the host innate immune system during UTIs. (ncl.ac.uk)

Transposon mutagenesis has been proven to be a powerful tool to identify genes and networks which are involved in survival and fitness under a given condition by simultaneously assaying the fitness of millions of mutants, thereby relating genotype to phenotype and contributing to an understanding of bacterial cell biology. (biorxiv.org)
AlbaTraDIS can identify genes which are up or down regulated, or inactivated, between multiple conditions, producing a filtered list of genes for further experimental validation as well as several accompanying data visualisations. (biorxiv.org)

Acquisitions of OspC genes that are novel for the region confer to recipients unique identities to host immune systems and, presumably, selective advantage when immunity to existing types is widespread among hosts. (cdc.gov)

The MICA allele is a polymorphic MHC class I-related A gene ( MICA ) family. (medscape.com)
Most loci contribute to both phenotypes, and both directional (consistently favouring one allele over the course of human history) and balancing (favouring the retention of both alleles within populations) selection effects are evident. (bioseek.eu)
Despite the gene's repetitive and GC-rich sequences, 13% of RPGR gene copies showed mutation correction and conversion to the wild-type allele. (nature.com)

Although the original disease-causing mutation would still be present in patient iPSCs, precise mutation correction is possible through gene editing techniques adapted from the bacterial clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system 3 . (nature.com)
In this study, we generated iPSCs from a patient with a mutation in the retinitis pigmentosa GTPase regulator ( RPGR ) gene, which causes an aggressive, X-linked variant of RP (XLRP). (nature.com)
We tested whether CRISPR could precisely edit the pathogenic mutation and produce gene-corrected iPSCs for eventual use in autologous transplantation. (nature.com)
1978. Appendix II: The bacterial mutation test. (cdc.gov)
Under the conditions of the study, the test article did not induce mutation at the hprt locus of L5178Y mouse lymphoma cells, in the presence and absence of S9 mix. (europa.eu)
Negative results were obtained in in vitro mammalian cytogenicity and gene mutation studies. (europa.eu)

For example, even if comprehensive insertion libraries are produced, it is inevitable that some genes, especially the shortest ones, could elude insertion and be spuriously annotated as essential, while transposon insertions that occur at gene ends and do not fully inactivate the function could lead to genes being incorrectly classified as non-essential. (biomedcentral.com)
a software application for rapid large-scale comparative analysis of TraDIS experiments that predicts the impact of transposon insertions on nearby genes. (biorxiv.org)

It is an example of positive control or regulation of gene expression in the bacterial operon. (nih.gov)

The multi-locus sequence type, presence of antimicrobial resistance (AMR) determinants, and relatedness between the isolates were derived from the sequence data. (who.int)

Yeast transformation allowed the introduction of a DNA molecule from any organism into yeast cells and enabled the manipulation of the expression of those genes. (genestogenomes.org)

On the host side, upregulation of inflammatory cytokine genes was observed along with pattern recognition receptor genes. (biomedcentral.com)
5 × 10-8) associated with chronic painful TMD, including loci near genes involved in the regulation of inflammatory and neuronal response. (iasp-pain.org)

This finding suggested that eukaryotes were not likely to have bacterial-style operons. (genestogenomes.org)

2006. Regulation of B-cell tolerance by the lupus susceptibility gene Ly108. (uh.edu)

Genes and Immunity 3: S13-S20. (uh.edu)
By acquisition of the complete part or a large part of a novel ospC gene, an otherwise adapted strain would assume a new serotypic identity, thereby being comparatively fitter in an area with a high prevalence of immunity to existing OspC types. (cdc.gov)

To investigate the response of LuxS01 and wild type strain to exogenously added AI-2, cells from Venetoclax in vitro experiments A and B were harvested in late exponential phase for RNA extraction and microarray gene expression analysis. (hsdpathway.com)

We also found that DNA double-strand breaks (DSBs) induce the approach of the homologous loci with γ-irradiation. (nature.com)
Intriguingly, an N-terminally truncated form of TIN2 lacking the TPP1 interaction domain can still induce significant telomerase-dependent telomere extension [ 8 , 22 ], suggestive of a TPP1-independent role for TIN2 in telomerase regulation. (prolekarniky.cz)

These results suggest that homologous loci approach each other to repair DSBs and AtRAD54 mediates these phenomena. (nature.com)

The MtING2 PHD finger bound H3K4me2/3 peptides weakly in vitro , but analysis of gene‐edited mutants indicated that it was dispensable to MtING2 function in wild‐type plants. (nsf.gov)
The substance is not genotoxic in vitro in bacterial or in mammalian cell systems. (europa.eu)
dimers, oligomeric reaction products with tall-oil fatty acids and triethylenetetramine is not mutagenic in bacterial cells or in mammalian cells in vitro . (europa.eu)
Accordingly, the specific aims of this application are: 1) In vitro mechanistic studies on virulence gene regulation by tryptophan derivatives. (grantome.com)

The recent completion of the human genome sequence allows genomics research to focus on understanding gene complexity, expression, and regulation. (ox.ac.uk)

dnaH is a gene involved in DNA replication. (wikipedia.org)

role of a plant ING2 gene in development, flowering, and gene expression, which likely involves an epigenetic mechanism. (nsf.gov)

We found that the distributions and frequencies of types of OspC genes differed between populations of B. burgdorferi in the Northeast, the Midwest, and California. (cdc.gov)

One live-cell imaging technique, a chromatin-tagging system that is based on the bacterial operator/repressor system, has been a powerful technique for analysing chromatin dynamics and arrangement in real time 11 , 12 . (nature.com)
We revealed that the homologous loci kept a constant three-dimensional distance in the nucleus using live-cell imaging with a bacterial operator/repressor system. (nature.com)
Mting2 mutants had reduced expression of activators of flowering, including the FT ‐like gene MtFTa1 , and increased expression of the candidate repressor MtTFL1c , consistent with the delayed flowering of the mutant. (nsf.gov)

Gene co-expression network analysis emphasizes this relationship, with pathways shared between host responses to mycobacteria and those predisposing to IBD. (bioseek.eu)

We identified the bacterial membrane bound histidine sensor kinase (HK) CpxA as a sensor of serotonin and indole. (grantome.com)

The main module page also shows the location of these motifs within the upstream sequences of the module member genes. (systemsbiology.net)
cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. (systemsbiology.net)

therefore, it is also important for gene regulation and DNA replication in A. thaliana as well as other organisms. (nature.com)

This regulation led to reduced EHEC adhesion to host cells. (nih.gov)
Through CpxA, both serotonin and indole decrease expression of the locus of enterocyte effacement (LEE) in EHEC, which encodes a type three secretion system (T3SS), effectors and an adhesin necessary for EHEC to form lesions on enterocytes leading to intestinal disease. (grantome.com)

RNA-seq data showed that FLK regulates expression abundance of some major defense- and development-related genes as well as alternative splicing of a number of genes. (nsf.gov)

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is one form of gene editing that can be used to precisely modify a gene of interest without otherwise compromising the favorable traits of an elite variety (Rani et al. (ufl.edu)
To generate healthy patient-derived cells, mutations might be repaired with new gene-editing technology based on the bacterial system of clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9, thereby yielding grafts that require no patient immunosuppression. (nature.com)

Anatomy14

Organisms4

Diseases1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes59

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Health Care1

Genomic5

Pathogenesis4

Quantitative trai1

Genetic14

Susceptibility loci3

Chromosome5

Varying phenotypes1

Virulence gene expression2

Bacteria4

Genomes2

Species4

Strains9

Activated the expression of fl1

Metabolism6

Pathogen5

Infection5

Encode2

Differentially3

Escherichia1

Reverse mutation assay1

Methylation4

Regulatory5

Vivo3

Pathogens2

Proteins6

Molecular4

Transcription3

Pathway3

Variants1

Bacterium1

Putative1

Genetics2

Interactions3

Protein3

Regulate1

Regulators2

Analyzing gene expression1

Artificial chromosomes2

Downstream1

Essential genes5

Resistance genes2

Innate1

Identify genes2

Confer1

Allele3

Mutation6

Transposon insertions2

Operon1

Antimicrobial resistance1

Organism1

Inflammatory2

Operons1

Tolerance1

Immunity2

Strain1

Induce2

Phenomena1

Vitro4

Sequence1

Involved in DNA replication1

Epigenetic1

Populations1

Repressor3

Pathways1

Histidine1

Sequences2

Replication1

EHEC2

Regulates expression1

Short Palindromic Repeats2