• Antagonism of PP2A by HIV-1 Vif is normally unbiased of APOBEC3 family members proteins as a result, and regulates cell routine development in HIV-infected cells. (estme.org)
  • Many host proteins can also function as cofactors for viral entry, such as heparin sulfate proteoglycans, C-type lectins, neuropilin-1 and furin [ 10 - 13 ]. (ijbs.com)
  • After being entry, the viral and host membranes can fuse together and then release the positive sense, single-stranded RNA genome of SARS-CoV-2 that directly translates into the structural and nonstructural proteins [ 9 ]. (ijbs.com)
  • We set out to identify the SARS-CoV-2 pathogenic proteins that through host interactions cause the cellular damages underlying COVID-19 symptomatology. (biomedcentral.com)
  • We examined each of the individual SARS-CoV-2 proteins for their cytotoxicity in HEK 293 T cells and their subcellular localization in COS-7 cells. (biomedcentral.com)
  • We also used Mass-Spec Affinity purification to identify the host proteins interacting with SARS-CoV-2 Orf6 protein and tested a drug that could inhibit a specific Orf6 and host protein interaction. (biomedcentral.com)
  • All three proteins showed membrane localization in COS-7 cells. (biomedcentral.com)
  • Our study revealed Orf6 as a highly pathogenic protein from the SARS-CoV-2 genome, identified its key host interacting proteins, and Selinexor as a drug candidate for directly targeting Orf6 host protein interaction that leads to cytotoxicity. (biomedcentral.com)
  • However, functions of individual SARS-CoV-2 viral proteins, particularly how they affect human cells, remain largely unknown. (biomedcentral.com)
  • Understanding how SARS-CoV-2 hijacks host machinery and which of its proteins are key for its interaction will be crucial in identifying effective targets for COVID-19 therapeutic intervention. (biomedcentral.com)
  • Studies of the human immune deficiency virus (HIV-1) over the past 30 years have demonstrated the presence of prime pathogenic proteins that contribute to virulence and host disease progression, and the potential of targeting these proteins for effective therapeutic intervention [ 9 ]. (biomedcentral.com)
  • For example, HIV-1 Vpr protein interacts with host proteins to modify host cell energy metabolism, oxidative status and proteasome function. (biomedcentral.com)
  • In the present study, we examined potential contributory roles of membrane-associated, cholesterol-enriched lipid rafts/caveolae and their constituent proteins (e.g., caveolin-1 [Cav-1]) as potential sites for IL-1β−induced nitric oxide (NO) release in the isolated β-cell. (diabetesjournals.org)
  • Therefore, the examine of host virus interactions, espe cially the influence on the regulation of apoptotic proc esses by the diverse viral proteins is poorly defined but might support clarify these difficulties. (pkainhibitors.com)
  • So, if viral proteins inhibit host cell apoptosis this impact may possibly contribute for the viral persistence because the virus escapes the immunologi selleckchem cal assault. (pkainhibitors.com)
  • However, if viral proteins induce apoptosis while in the host cell, this may be an essential factor for liver cell destruction. (pkainhibitors.com)
  • As a result, some viruses are able to induce apoptosis in the host cell by way of their newly synthesized virus specific proteins, when virus unique proteins from other viruses act as anti apoptotic agents. (pkainhibitors.com)
  • These genes encode different alleles of a coagulase, the von Willebrand factor-binding protein, which has a role in determining the animal host specificity of S. aureus. (wikipedia.org)
  • Besides new methods for the study of conformational dynamics of biomolecules and protein folding, direct stochastic optical reconstruction microscopy ( d STORM) has been developed that enables super-resolution fluorescence imaging with standard organic fluorophores with a spatial resolution of ≤ 20 nm in the imaging plane in fixed and in living cells (Figure) [8]. (uni-wuerzburg.de)
  • Besides the function in viral life cycle, N protein can bind GSDMD to antagonize pyroptosis but promotes cell death via the Smad3-dependent G1 cell cycle arrest mechanism. (ijbs.com)
  • In this review article, we are focusing on the signaling mechanisms of SARS-CoV-2 N protein in viral replication, cell death and inflammation. (ijbs.com)
  • Of them, SARS-CoV-2 S protein binds to its cellular receptor, angiotensin-converting enzyme 2 (ACE2) [ 8 , 9 ], to enter the cells. (ijbs.com)
  • Additionally, the host serine protease TMPRSS2 is important for priming of the S protein for receptor interactions and entry [ 9 ]. (ijbs.com)
  • At the loxP site, plasmid DNA can be cleaved by the Cre protein of the P1 phage, at the cos site by the corresponding enzyme of the lambda phage. (genomequebecplatforms.com)
  • This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. (thermofisher.com)
  • Immunologic and confocal microscopic evidence also suggested a transient but significant stimulation of tyrosine phosphorylation of Cav-1 in β-cells briefly (for 15 min) exposed to IL-1β that was markedly attenuated by three structurally distinct inhibitors of protein tyrosine phosphorylation. (diabetesjournals.org)
  • E. g. in transfected HepG2, Jurkat T or COS seven cells endog enously expressing the core protein or even the total length HCV polyprotein, induction of apoptosis was observed. (pkainhibitors.com)
  • In contrast, stably transfected B cells expressing the core protein did not exert any apoptotic effect. (pkainhibitors.com)
  • Unlike the bacterial viruses, however, integrated SaPIs are mobilized by host infection with "helper" bacteriophages (specific SaPIs may require specific helper bacteriophages for mobilization, though Staphylococcus phage 80alpha appears to mobilize all known SaPIs). (wikipedia.org)
  • Alternative cargos include CRISPR/dcas9 with spacers targeting and inhibiting genes that regulate bacterial virulence, or the gene for lysostaphin, a powerful staphylolytic enzyme. (wikipedia.org)
  • The vectors of this series contain genes of the moderate bacteriophage P1, which ensure replication of the phage chromosome in infected bacterial cells. (genomequebecplatforms.com)
  • Recombinant DNAs based on them (insert size 150-200 kbp) are introduced into bacterial cells using electroporation. (genomequebecplatforms.com)
  • However, unlike the bacteriophage, which, during a latent (lysogenic) state, integrates its chromosome into the chromosome of the host bacteriophage, the P1 phage maintains the chromosome in the cytoplasm of bacterial cells in the form of a circular covalently closed molecule resembling a plasmid, the size of which is 100 kbp. (genomequebecplatforms.com)
  • A bacterial artificial chromosome (BAC) is a vector system based on the E. coli F-plasmid, cos sites of the lambda phage and loxP of the P1 phage, used for cloning long (150-350 kbp) DNA sequences. (genomequebecplatforms.com)
  • Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
  • Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
  • When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
  • Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
  • In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
  • They also carry other superantigen toxins as well as other virulence factors among which is a set of genes enabling their host strains to coagulate farm animal blood plasma. (wikipedia.org)
  • The F-plasmid encodes genes that regulate replication and control copy number (1-2 molecules per cell). (genomequebecplatforms.com)
  • After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
  • To resolve misconfigured cells we include a Bayesian fitted online artificial that is a different restriction to perform the genetic and the yeast genes, Qbeing P-splines. (scoutconnection.com)
  • Together, these results suggest that, in neurons, the GAKIN-PIP 3 BP complex transports PIP 3 to the neurite ends and regulates neuronal polarity formation. (silverchair.com)
  • online artificial photosynthesis 2016 cell and adjacent pLT42 transformed by Cre hash in general solutions, 2001 PNAS, 98:9209-14. (scoutconnection.com)
  • online artificial photosynthesis 2016: E22The applications for the complementary Building of fluid and resistive parameters are generally reasonably defined for a competing such indicator and a other construct cell. (scoutconnection.com)
  • SaPIs are used by the host bacteria to co-opt the phage reproduction cycle for their own genetic transduction and also inhibit phage reproduction in the process. (wikipedia.org)
  • These phage interference functions are advantageous both to the host cell, as they (partially) block predation by exogenous phages, and to the SaPI, as they enable it to keep pace with helper phage reproduction. (wikipedia.org)
  • Gaussian online artificial photosynthesis in Human Cells Catalyzed by Phage polynucleotide Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of event Mu can promote supernatant health in spline pairs, 1991 Mol. (scoutconnection.com)
  • The Beilhack group has been investigating immune cell interactions after allogeneic hematopoietic cell transplantation (HCT). (uni-wuerzburg.de)
  • Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
  • In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
  • Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
  • The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
  • The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
  • Leukocytic, hematopoietic cells are important in maintaining the body's defense against disease. (justia.com)
  • Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
  • These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
  • The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
  • The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
  • In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
  • PML occurs mainly in acquired immunodeficiency syndrome (AIDS) patients and less frequently after solid organ or allogeneic hematopoietic stem cell transplantations and in Multiple Sclerosis or Crohn's disease patients treated with immunomodulatory drugs [ 7 ]. (biomedcentral.com)
  • Much like bacteriophages, SaPIs can be transferred to uninfected cells and integrate into the host chromosome. (wikipedia.org)
  • COS-tat cell clones during long-term culture, while it is also likely that the Tat-expression construct is integrated into the host cell chromosome. (dna-pksignal.com)
  • J Cell Biochem 72:94-102. (cdc.gov)
  • Continuous interleukin-6 application in vivo via macroencapsulation of interleukin-6-expressing COS-7 cells induces massive gliosis. (uni-bielefeld.de)
  • The limited viral genome compels the virus to enlist host systems for its cellular infection, translation, replication and spread. (biomedcentral.com)
  • In PC12 cells and cultured hippocampal neurons, transport activity of GAKIN contributes to the accumulation of PIP 3 at the tip of neurites. (silverchair.com)
  • Bone marrow stromal cells reduce ischemia-induced astrocytic activation in vitro. (uni-bielefeld.de)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • The β-chemokines form a gradient that serves as a chemoattractant and potential proliferation signal for immune and other cells such as monocytes, macrophages, basophils, eosinophils, T lymphocytes and fibroblasts. (justia.com)
  • It is well established that inflammatory cytokines such as interleukin-1β (IL-1β) play a major regulatory role in the selective destruction of insulin-producing β-cells, resulting in the onset of type 1 diabetes ( 1 - 3 ). (diabetesjournals.org)
  • Furthermore, CD4 and CD8 T cells are concerned during the inflammatory system at the same time as the destruction of those cells by straight our site inducing cytotoxic effects through apop tosis or indirectly by secretion of various cytokines. (pkainhibitors.com)
  • Chemokines," which take their name from chemotactic cytokines, are small secreted polypeptides that regulate movement of immune cells into tissues (Baggiolini et al. (justia.com)
  • To address these complex challenges of a dysfunctional immune system caught between defective tolerance and compromised immunity we employ optical imaging methods, i.e. optical techniques that provide single cell resolution in the context of intact tissues. (uni-wuerzburg.de)
  • Yet, if tissue clearing is necessary, fixation of the tissues has limited real-time life cell imaging deep within tissues. (uni-wuerzburg.de)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • 18:217-242), the β-chemokines regulate the expression of adhesion molecules on endothelial cells and thereby indirectly affect diapedesis and extravasation of immune cells from the circulation into tissues. (justia.com)
  • Other shared functions of MCP-1, MCP-2, and MCP-3 include induction of N-acetyl β-D-glucosaminidase release, gelatinase B release, and granzyme A release which are believed to help the cells digest the extracellular matrix components necessary to enable them to migrate into tissues (Van Coillie et al. (justia.com)
  • Li-na ZHU , Chun-lei MEI , Zhong-xin ZHANG , 2008: The Function of SeMNPV IAP3 in Mammalian Cells, Virologica Sinica, 23, 183-188. (virosin.org)
  • 刘超红 , 焦成松 , 陈新文 , 2006: Cloning of HCV ns2 Gene and its Expression in Prokaryotic and Mammalian cells, Virologica Sinica, 21, 217-220. (virosin.org)
  • Effects of polychlorinated biphenyls on dopamine release from PC12 cells. (cdc.gov)
  • Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). (cellsignal.com)
  • While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
  • These residues covary across HIV-1 infections in vivo, favouring depletion of PPP2R5A-E. Through evaluation of stage mutants and taking place Vif variations, we further present that degradation of PPP2R5 family members subunits is normally both required and enough for Vif-dependent G2/M cell routine arrest. (estme.org)
  • To our knowledge, the results of the present study constitute the first demonstration of increased propagation of PML-type JCV in long term-culture of cell lines stably expressing HIV-1 Tat. (dna-pksignal.com)
  • Yet, to date allogeneic HCT is facing three major challenges: First, adequate immune reconstitution and control of infections, second, overreaching immune responses that can result in life-threatening graft-versus-host disease and, third, impaired immune effector functions that are not sufficient to control the underlying disease, e.g. eliminating remaining leukemic cells. (uni-wuerzburg.de)
  • As systemic CD8+ T-cell memory is critical in controlling LM infection, we addressed the issue as Ibrutinib chemical structure to whether it is modulated during pregnancy. (dna-pksignal.com)
  • a normal Listeria-specific adaptive immunity in particular CD8+ T-cell memory response suggesting that systemic listeriosis during pregnancy may be an immunopathology associated with placental infection. (dna-pksignal.com)
  • Furthermore, we developed a novel multicolour light-sheet fluorescence microscopy (LSFM) approach for deciphering immune and infection processes in large tissue specimens on a single-cell level in 3 dimensions [7]. (uni-wuerzburg.de)
  • Moreover, LM infection in pregnant and non-pregnant hosts evoked strong antigen-specific cytolytic CD8+ T cells that produced IFN-γ. (dna-pksignal.com)
  • It's been reported that induction of apoptosis is of good impor tance for your pathogenesis, and two big complications of HCV infection could possibly be relevant to apoptosis, i. e. the viral persistence as well as the direct or indirect destruction of liver cells. (pkainhibitors.com)
  • The Tat-expression plasmid (pcDNA-tat86) contains SV40 ori and is able to replicate in COS-7-derived cells expressing SV40 T antigen. (dna-pksignal.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
  • High-resolution microscopy of three dimensional environments has become powerful tools to dissect host-pathogen interactions. (uni-wuerzburg.de)
  • We will develop set-ups to study host-pathogen interactions in 3D tissue models under conditions closely approximating those of a natural environment. (uni-wuerzburg.de)
  • The GRK consortium provides an ideal environment to foster interdisciplinary collaboration and highly innovative research to quantitatively elucidate host-pathogen interactions at unprecedented molecular, cellular, microanatomical and functional detail in a unique setting that mimics the onset and progression of infectious diseases in a human tissue environment. (uni-wuerzburg.de)
  • Cells were labeled with antibodies against ß-tubulin (green) and Alexa 647 phalloidin (magenta). (uni-wuerzburg.de)
  • Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
  • The invention also provides cells producing the antibodies, and methods of making and using the same. (justia.com)
  • 1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
  • True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
  • Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
  • Human MCP-1, MCP-2 and MCP-3 all have chemotactic activity for a variety of cell types, including T lymphocytes and monocytes (Van Coillie et al. (justia.com)
  • C) Jurkat cells were labeled with an anti-ceramide C16 antibody and a secondary Alexa 647 labeled antibody. (uni-wuerzburg.de)
  • 5% cold fish gelatine and 0,1% ovalbumin 1x PBS for thirty min at RT plus the cells have been washed again 3 × 5 min with 1x PBS at RT, followed by the major antibody at four C overnight. (liverxreceptor.com)
  • Strikingly, the vital residues for PPP2R5 depletion discovered in our display screen included many previously driven to make a difference for Vif-dependent cell routine arrest in various other, unbiased research (31, 33, 44) (DeHart et al. (estme.org)
  • We had been originally puzzled because some Vif stage mutants had been markedly impaired within their ability to trigger cell routine arrest, yet maintained the capability to deplete at least some PPP2R5 family members subunits. (estme.org)
  • Furthermore, the power of Vif to trigger cell routine arrest didn't may actually correlate with depletion of anybody, particular PPP2R5 subunit. (estme.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Cellular RNAs are regulated at multiple stages, including transcription, RNA maturation and degradation. (nature.com)
  • early, during synthesis repeated cells used with microRNA transcription left rather taken for these methods. (scoutconnection.com)
  • This course examines the theories of host-parasite coevolution, including optimal host resource allocation to immune defense in light of parasite counter-strategies, and assesses the empirical evidence by which these theories are tested. (princeton.edu)
  • Finally, students will map immune mechanisms onto host phylogenies to understand the order in which different mechanisms arose over evolutionary time. (princeton.edu)
  • The moment the preferred serum titer was attained, immune spleens were removed through the mice, dissociated, and fused with SP2 o myeloma cells. (liverxreceptor.com)
  • Actually, because effective depletion of most PPP2R5 subunits must halt cell routine progression, they are not really Meclofenoxate HCl paradoxes in any way. (estme.org)
  • A subset of SaPIs have acquired a cos site and use the phage's TerS for packaging by the cos mechanism. (wikipedia.org)
  • 王平忠 , 白雪帆 , 潘 蕾 , 张颖 , 黄长形 , 2003: Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome, Virologica Sinica, 18, 420-422. (virosin.org)
  • How ever, conflicting data have been produced based on the experimental problems, i. e. procedures and cell lines utilized. (pkainhibitors.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • In human cell lines, tunicamycin enhances cytotoxicity stimulated by cisplatin. (sigmaaldrich.com)
  • 汪艳 , 刘春新 , 易有荣 , 崔冶建 , 朱珺 , 董长垣 , 2006: Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods, Virologica Sinica, 21, 253-256. (virosin.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Tunicamycin has also been used to study the functional effects of coxsackievirus and adenovirus receptor (CAR) glycosylation in COS-7 cells. (sigmaaldrich.com)
  • Disruption of lipid rafts (e.g., with cyclodextrin) markedly reduced IL-1β−induced gene expression of inducible NO synthase (iNOS) and NO release from β-cells. (diabetesjournals.org)
  • Overexpression of an inactive mutant of Cav-1 lacking the tyrosine phosphorylation site (Y14F) or an siRNA-mediated Cav-1 knock down also resulted in marked attenuation of IL-1β-induced iNOS gene expression and NO release from these cells, thus further implicating Cav-1 in this signaling cascade. (diabetesjournals.org)
  • Stable expression of Tat is an important feature for generating JCV propagation system using COS-tat cells. (dna-pksignal.com)
  • Effects of astrocyte-targeted production of interleukin-6 in the mouse on the host response to nerve injury. (uni-bielefeld.de)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • While completing original research, you will employ techniques used by cell and molecular biologists and developmental geneticists. (princeton.edu)
  • From many different viruses it really is renowned that they employ various apoptotic signaling elements inside the host cell for inhibition or activation of your endogenous suicide program. (pkainhibitors.com)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
  • The released SaPI particles infect other staphylococcal cells, injecting their DNA which integrates into a specific chromosomal attachment site of which there are 5 in S. aureus. (wikipedia.org)
  • Until now, 14 polyomaviruses with human hosts have been identified. (biomedcentral.com)
  • 5)Cell and Molecular Biology by Phillip Sheeler, Donald E. This free Designing for Human was shown on July 26, 2012 by Appendix. (scoutconnection.com)
  • We found that Orf6, Nsp6 and Orf7a induced the highest toxicity when over-expressed in human 293 T cells. (biomedcentral.com)
  • Treatment with Selinexor, an FDA-approved inhibitor for XPO1, attenuated Orf6-induced cellular toxicity in human 293 T cells. (biomedcentral.com)
  • Tunicamycin has been used to study the effect of N-linked glycosylation of human proton-coupled folate transporter (HsPCFT) in HeLa cells. (sigmaaldrich.com)
  • Important concepts and elements of molecular biology, biochemistry, genetics, and cell biology, are examined in an experimental context. (princeton.edu)