Recombination genes mice knockout. Medical search. Frequent questions

He developed a technique called homologous recombination that introduced targeted genetic modifications to cells and allowed the creation of so-called 'knockout mice' to help scientists understand how individual genes work. (wral.com)
To generate loss of function knockout mouse we will use gene-targeting technology to disturb the endogenous Zfyve27 gene of the mouse through homologous recombination in embryonic stem (ES) cells. (hsp-info.de)
To produce the loss of function mouse model for ZFYVE27, we employed a novel Bacterial Artificial Chromosome (BAC) mediated homologous recombination method to generate the knockout construct in C57BL/6 background. (hsp-info.de)
The BAC mediated homologous recombination technique is based upon the Red/GET recombination, in which the bacteriophage recombination genes are genetically engineered in E. coli to efficiently manipulate the BAC containing gene of interest with ease in short period of time. (hsp-info.de)
This method uses homologous recombination to disable a gene of interest to produce a genetic knockout. (cshl.edu)
In mammals, this process relies on the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) that generates crossover recombination events (COs) [ 1 ]. (nature.com)
Along with a recent study by Hannon and his colleagues that demonstrated germline transmission of RNAi, the current study establishes RNAi as a convenient alternative to traditional, laborious, and less flexible homologous recombination-based gene knockout strategies for studying the effects of reduced gene expression in a wide variety of settings. (sciencedaily.com)
The system is directed toward the production of gene targeting constructs by homologous recombination, and by methods generating transgenic animals. (cellmedicine.com)
The present invention is directed to methods for producing gene targeting constructs by homologous recombination using mouse genomic libraries arrayed in yeast shuttle vectors. (cellmedicine.com)
Two of these-homologous recombination (HR) and non-homologous end-joining (NHEJ)-are different routes for fixing the supposed damage. (the-scientist.com)
AND-34 −/− mice were generated by homologous recombination. (molvis.org)
The gene-targeting construct was launched into embryonal stem (ES) cells and heterozygous cyclin A2f/+ (Af denotes XI-006 the "floxed" allele) ES were obtained through homologous recombination (Figures 1A and 1B). (immune-source.com)
We characterized the importance of the homologous recombination aspect RAD54 for the developing mouse human brain cortex in normal circumstances or after ionizing rays exposure. (academicediting.org)
In the developing human brain RAD54-reliant homologous recombination made an appearance absolutely necessary for the fix of problems induced by ionizing rays during S and G2 stages however not for the fix of endogenous problems in normal conditions. (academicediting.org)
Entirely our data support the existence of -independent and RAD54-dependent homologous recombination pathways. (academicediting.org)
Nonhomologous end-joining (NHEJ) and homologous recombination (HR) constitute the two main pathways to repair DSB in mammalian cells. (academicediting.org)
This technology, commonly referred to as gene targeting or knockout, is based on homologous recombination between DNA sequences residing in the chromosome and newly introduced DNA to mutate any genes of interesting in mouse genome [ 1 ]. (ijbs.com)
This work led to his important conclusion that mammalian somatic cells possess an efficient enzymatic machinery for mediating homologous recombination [ 6 ]. (ijbs.com)
He reasoned that if this machinery could be efficient for homologous recombination between a newly introduced DNA molecule and the endogenous DNA sequence, any host gene could be mutated. (ijbs.com)
The correction occurred in one cell per 1,000 injected cells, a relatively high frequency, which makes it possible to use homologous recombination to manipulate endogenous genes of the mammalian genome. (ijbs.com)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)

The mismatch repair (MMR) pathway plays an important role in the maintenance of the genome integrity, meiotic recombination and gametogenesis. (biomedcentral.com)
Studies of gene knockout mice indicate that several members of the MMR family also participate in the meiotic recombination process and are involved in gametogenesis [ 17 , 18 ]. (biomedcentral.com)

In conditional knockout mice, a gene is inactivated in a specific tissue to study the function of individual genes and model human diseases. (cyagen.com)
Like Cre/LoxP system, the FLP/FRT system has been widely used for gene expression and generating conditional knockout mice, mediated by the FLP/FRT system. (vectorbiolabs.com)

The CIDEB knockout mice are resistant to high fat diet induced obesity and liver steatosis. (wikipedia.org)
It was previously reported that FFAR1 knockout ( Ffar1 −/− ) mice were resistant to high-fat diet-induced hyperinuslinemia, hyperglycemia, hypertriglyceridemia, and hepatic steatosis. (diabetesjournals.org)

Attenuated nephritis in inducible nitric oxide synthase knockout C57BL/6 mice and pulmonary hemorrhage in CB17 SCID and recombination activating gene 1 knockout C57BL/6 mice infected with Leptospira interrogans. (fiocruz.br)
Firstly, we identified two BAC genomic clones (RPCIB731C16470Q2 and RPCIB731A01447Q2) from the mouse genomic DNA library RPCI-23, which is derived from the C57BL/6 mouse strain containing full genomic sequence of Zfyve27 and 50 kb flanking sequence from either end of the gene. (hsp-info.de)
This study is designed to investigate FFAR1 function in vivo using a third line of independently generated Ffar1 −/− mice in the C57BL/6 background. (diabetesjournals.org)

A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. (nih.gov)
Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
This year, the Nobel Prize in Physiology or Medicine goes to Drs. Mario R. Capecchi, Martin J. Evans and Oliver Smithies for their discoveries of principles for introducing specific gene modifications in mice by the use of embryonic stem (ES) cells. (ijbs.com)

The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. (nih.gov)
The KO-first strategy has implications for functional genomics and proteomics across many model systems, including mouse, rat, and human pluripotent stem cells - serving as a functional genomics platform enabling systematic, genome-scale programs for proteomic mapping. (cyagen.com)
Since then, gene targeting has proven to be a powerful means of precisely manipulating the mammalian genome, generating at least ten thousand mutant mouse stains. (ijbs.com)
Here, we review the arrival of genetic screens in malaria parasites to analyse parasite gene function at a genome-scale and their impact on understanding parasite biology. (portlandpress.com)
The remaining recombination sites will be located in non-conserved regions of the genome. (taconic.com)

The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)
To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), were bred to the Rosa26R (R26R) reporter. (diabetesjournals.org)
Inducible and/or tissue-specific gene expression - knockout, over-expression, etc. (cyagen.com)
The Cre-Lox recombination system enables the generation of tissue-specific or inducible knockouts with a high level of control over the spatial and temporal expression of genes. (cyagen.com)

In conventional knockout models, one or more critical exons of the target gene are replaced with a drug-selection cassette. (cyagen.com)
In the Cre-Lox model development strategy, one or more critical exons of the target gene are flanked by LoxP sequences - known as the floxed gene - which can be recognized by Cre recombinase. (cyagen.com)
The BTK gene is large and consists of 19 exons that encode the 659 amino acids that form the Btk cytosolic tyrosine kinase. (medscape.com)

Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. (portlandpress.com)

Transgene expression using viral vectors provides an alternative, which allows for temporal expression of genes in mice with reduced cost and effort. (nature.com)

In addition, the aP2-Cre line can lead to germline recombination of floxed alleles in ∼2% of spermatozoa. (diabetesjournals.org)

For example, mice genetically deficient for adaptive immunity displayed accelerated bone healing. (hindawi.com)

Dot1l transcripts are abundant in mouse kidney and contain five alternative splicing variants (Dot1a-e) [17]. (bioskinrevive.com)
In addition, it expands the spectrum of pathogenic variants in the C12orf57 gene. (bvsalud.org)
The purpose of this work is three-fold: (1) to examine whether MMR gene polymorphisms are associated with increased risk of azoospermia or oligozoospermia, (2) to ascertain whether genetic variants in MMR genes result in sperm DNA damage and, thereby, increase male infertility, and (3) to investigate the biological activity of the significant functional variants. (biomedcentral.com)

This invention introduces novel methods and compositions for the preparation of a genomic library, specifically a mouse genomic library, for producing knock-out targeting vectors by arraying into pools bacteria transformed with the yeast-Escherichia coli shuttle vector comprising mouse genomic DNA fragments. (cellmedicine.com)
Further disclosed are the procedures for preparing a genomic library for use in producing knockout targeting vectors, including details of genomic DNA fragments, bacterial selection markers, yeast replication, and the amplification of shuttle vectors. (cellmedicine.com)

This model is amenable to additional genetic modifications and deletion of another cancer-related gene, Mmp9 , predictably alters the tumor microenvironment and facilitates cancer progression. (biologists.com)
Most mice with the whole-body TAZ deletion died before birth, apparently because of skeletal muscle weakness. (ubigene.us)
Physique 1 Generation of Cyclin A2f/f Mice In order to verify that deletion of the "floxed" cyclin A2 sequences resulted in a functionally null allele we crossed cyclin A2f/f mice with a "deleter" Meox2-Cre strain (Tallquist and Soriano 2000 and generated cyclin A2? (immune-source.com)
Using Hprt and the cystic fibrosis transmembrane conductance regulator ( Cftr ) genes, Capecchi and colleagues demonstrated that specific alterations (i.e. the F508 of CFTR, small deletion or insertion, and later, a loxP site for conditional mutations) that are placed several kb away from the selection marker could be co-transferred into ES cells through a double crossover event [ 12 ]. (ijbs.com)

A comprehensive expression analysis of ZFYVE27 in the total protein extract isolated from various tissues of mouse revealed a high level of expression primarily in the HSP affected tissues such as the brain, cerebellum and spinal cord. (hsp-info.de)
The Adipoq-Cre exhibited no observable recombination in any other tissues examined, whereas both aP2-Cre lines resulted in recombination in endothelial cells of the heart and nonendothelial, nonmyocyte cells in the skeletal muscle. (diabetesjournals.org)
In this study, we found heightened expression levels of GAC in early AD mouse brain tissues compared with those in control littermates. (shengsci.com)
By breeding floxed mice with tissue-specific Cre-expressing mice (Cre deleter strain), it is possible to specifically delete the floxed region and inactivate the gene in desired tissues, while the target gene remains functional in all other tissues. (cyagen.com)
Similar to lymphomas that form in the absence of p53, lymphomas that formed in mice with low p53 levels developed rapidly (reaching terminal stage after 66 days, on average), infiltrated lung, liver, and spleen tissues, and showed little apoptosis or "programmed cell death. (sciencedaily.com)
In contrast, lymphomas that formed in mice with intermediate p53 levels developed less rapidly (reaching terminal stage after 95 days, on average), did not infiltrate lung, liver, or spleen tissues, and showed high levels of apoptosis. (sciencedaily.com)

However, the recombination efficiency of FLPe in cells remains very low relative to other recombinases because of its non-mammalian origin. (vectorbiolabs.com)

Our analysis suggests that the identified mutation in SPG33 family will most likely result in a loss of function of the protein due to the misfolding of C-terminal FYVE finger domain therefore a knockout mouse will be an appropriate model system to study this disease. (hsp-info.de)
the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
Barth syndrome is a rare metabolic disease in boys caused by mutation of a gene called tafazzin or TAZ. (ubigene.us)
We previously published a proof-of-principle of the efficiency of AAV-mediated transfer of the FKRP gene to correct the myopathology in a KI mouse model reproducing the most frequent mutation (L276I) in LGMD-R9. (mdaconference.org)
To demonstrate this principle, he generated recipient cell lines carrying an artificial target, a defective neomycin resistance gene that was stably integrated into host chromosome, and was able to repair it by injecting the same gene carrying a different mutation into the nucleus of these cells [ 7 ]. (ijbs.com)
Introduction of these types of mutation is a challenge in the early stages of gene targeting. (ijbs.com)
A minimum of four (4) mice mutant for the selected mutation. (taconic.com)

Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. (diabetesjournals.org)
Mice lacking TAZ just in their cardiac tissue, which all survived to birth, showed the same features. (ubigene.us)
For conditional knockouts, it may be possible for Cyagen to breed founders directly to your tissue-specific Cre mice or even develop the Cre strains necessary for your study. (cyagen.com)
A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
The system allows for the generation of mice with specific overexpression of AAVR through breeding with available Cre mouse lines, as well as the option to detarget AAV from other tissue when paired with Aavr -KO mice. (nature.com)

This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. (nih.gov)
Rad51b c.92delT/c.92delT mice exhibited meiotic DNA repair defects due to RAD51 and HSF2BP/BMRE1 accumulation in the chromosome axes leading to a reduction in the number of crossovers. (nature.com)

CIDEB knockout mice have been generated by homolog recombination technique. (wikipedia.org)

This line was used to generate connecting tube/collecting duct (CNT/CD)-specific or mice [29] which drive Cre recombinase expression under the control of regulatory elements of the mouse gene. (bioskinrevive.com)
In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice. (taconic.com)

In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
Studies of these mutant mice have yielded enormously useful information in virtually all fields of biological and biomedical sciences. (ijbs.com)
Indeed, gene targeting in ES cells can theoretically be used to generate mutant mice for all genes in the near future. (ijbs.com)

To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
The impact of sphingolipids on MDR1 gene expression in cancer appears to reflect the myriad intracellular signaling pathways of ceramide and its metabolites. (sphingolipidclub.com)

The 'knockout-first' (KO-first) allele overcomes the limitations of constitutively-expressed mutations, flexibly producing reporter knockouts, conditional knockouts, and null alleles through exposure to site-specific cyclization recombination (Cre) and flippase (FLP) recombinases - such as in crosses to FLP and cre mice. (cyagen.com)
Knockout-first alleles can also be readily modified in cells using dual recombination-mediated cassette exchange. (cyagen.com)
The KO-first allele design also forms the basis for generation of lacZ-tagged conditional alleles as part of the International Knockout Mouse Consortium (IKMC) effort organized by the International Mouse Phenotyping Consortium (IMPC). (cyagen.com)

RESULTS Generation and Characterization of Conditional Cyclin A2 Knockout Mice To obtain a conditional cyclin A2 allele we inserted sites into the first and seventh intron of the murine cyclin A2 gene (Physique 1A). (immune-source.com)
2003 Cyclin A2f/f mice were viable and phenotypically normal (data not shown) consistent with our expectation that this "floxed" cyclin A2 allele is usually functionally wild-type. (immune-source.com)
The constitutive KO allele can be generated by Cre-mediated recombination. (taconic.com)

amyloid peptide, a major constituent of the amyloid plaque, can result from mutations in the gene encoding amyloid precursor protein, a protein which when normally processed will not produce the .beta. (justia.com)
CHARGE (Coloboma of the eye, Heart defects, Atresia of the choanae, Retardation of growth, Genital anomalies and Ear abnormalities) syndrome is a disorder caused by mutations in the gene encoding CHD7, an ATP dependent chromatin remodelling factor, and is characterised by a diverse array of congenital anomalies. (bvsalud.org)
Mutations in the gene coding for Fukutin related protein (FKRP) can lead to different diseases, ranging from very severe multi-organ syndromes like Walker Warburg Syndrome (WWS) and Muscle Eye Brain disease (MEB), to muscle defects as Congenital Muscle Dystrophy (CMD) and the mildest but more frequent form Limb Girdle Muscle Dystrophy type 2i (LGMD2i or LGMD-R9 FKRP-related). (mdaconference.org)

By employing a conditional knock-in approach using the human EGFR cDNA mice humanised for EGFR ( hEGFR KI/KI )were generated. (silverchair.com)

Here we generate a robust system for in vivo transgene delivery by combining the versatility of AAV-mediated transgene delivery with the specificity of cell-type-specific Cre-mediated recombination. (nature.com)

In the targeting vector, we will replace the endogenous gene with green fluorescent protein, which will enable us to determine the endogenous expression pattern of Zfyve27. (hsp-info.de)
These studies led to his successful targeted disruption of the first endogenous gene, Hprt, in ES cells in 1987, as mentioned earlier [ 2 ]. (ijbs.com)

In humans, individuals carrying rare loss of function mutations in the CIDEB gene are protected against different aetiologies of liver disease. (wikipedia.org)
Because humans share the vast majority of their genes with mice, gene-targeted mice are used to reproduce diseases that occur in humans. (utah.edu)
1983 Subsequently cyclin A genes have been found in all multicellular organisms including humans (Pines and Hunter 1990 While only a single cyclin A gene is present in the genomes of and cultured fibroblasts XI-006 or other cell types blocked DNA synthesis consistent with the essential function for cyclin A in DNA replication (Girard et al. (immune-source.com)
Mouse models have milder disease than humans. (medscape.com)
The demonstration of what could happen was great, and they used mice because it's a more tractable system, but what we cared about were humans. (medscape.com)

Although conditional knockout (cKO) mouse models are used in similar applications to KO models, conditional models provide precise control of gene expression to yield greater experimental capabilities. (cyagen.com)
Compared with constitutively expressed gene mutations, conditional models provide greater temporal and regional control of gene expression. (cyagen.com)
cKO models allow precise control of gene expression for a functional protein target, providing an in vivo system to evaluate potential off-target effects of a drug. (cyagen.com)
In the mouse model, forced expression of the Myc oncogene in B-cells causes the mice to develop B-cell lymphomas by 4 to 6 months of age. (sciencedaily.com)
The HSA-FKRPdel mouse model was then used for a dose effect study of an AAV9 expressing FKRP under the expression of a muscle promoter. (mdaconference.org)
In the present study we examined the relationship between ceramide, ceramide metabolites and expression of the MDR1 gene in human breast cancer cell lines. (sphingolipidclub.com)
It has been shown that approximately 2 kb of 5'-flanking DNA of the human GFAP gene is sufficient to direct transgene expression to astrocytes and confer developmental/injury-induced regulation. (vectorbiolabs.com)
FLPo is a codon-optimized version of FLPe that greatly increases the protein expression and the FRT recombination efficiency in mouse cells. (vectorbiolabs.com)

Furthermore, the adaptation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated protein 9 (CRISPR/Cas9) technology has dramatically improved gene editing efficiency at the single gene level. (portlandpress.com)
Subsequent screening for thermostable mutants resulted in the identification of FLPe which has a 4-fold higher recombination efficiency at 37oC than original FLP. (vectorbiolabs.com)

The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
The polyuria phenotype in mice and in patients with DN may be partially due to upregulated Aqp5. (bioskinrevive.com)
Outcomes mice and explanation of their polyuria phenotype on a standard pellet Na+ diet plan are detailed inside our related manuscript [30]. (bioskinrevive.com)
Early Retinal Defects in Fmr1-/y Mice: Toward a Critical Role of Visual Dys-Sensitivity in the Fragile X Syndrome Phenotype? (shengsci.com)
Since the phenotype of this model is relatively modest, we developed a muscle specific FKRP knock-out mouse model, based on Cre-lox recombination technology. (mdaconference.org)
Moreover, hEGFR KI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr -/- mice and has not been previously described. (silverchair.com)

The study illustrates the ease with which RNAi "gene knockdowns" can be used to create a full range of mild to severe phenotypes (something that geneticists dream about), as well as the potential of RNAi in developing stem cell-based and other therapeutic strategies. (sciencedaily.com)

Although the mechanisms of gene activation are still unclear, ceramide's role as a messenger of cytotoxic response to chemotherapy might be linked to the MDR pathway. (sphingolipidclub.com)

Inoculation of RAG-2 null mice with multiple myeloma cells causes tumor formation in the bone marrow and osteolytic bone disease, similar to human patients. (biologists.com)
Quickly we utilized a conditional knockout range (range [29] to inactivate and therefore abolish histone H3 K79 methylation in Aqp2-expressing cells which can be found in the CNT/Compact disc [30]. (bioskinrevive.com)
Repair of mitomycin-C-induced chromosomal aberrations was impaired in RAD51B/Rad51b-c.92delT human and mouse somatic cells in vitro and in explanted mouse bone marrow cells. (nature.com)
Transduction of Adeno-Associated Virus Vectors Targeting Hair Cells and Supporting Cells in the Neonatal Mouse Cochlea. (shengsci.com)
Getting the g ene in Further tests showed that TAZ gene therapy provided durable treatment of the animals' cardiomyocytes and skeletal muscle cells, but only when at least 70 percent of heart muscle cells had taken up the gene. (ubigene.us)
This results in the permanent inactivation of the target gene in all cells of the body throughout development. (cyagen.com)
Now, researchers at CSHL have shown that RNAi can be used to set the level of gene activity in stem cells on "low," "medium," or "high. (sciencedaily.com)
The new study indicates that stable suppression of deleterious genes by RNAi--in which adult stem cells are isolated, modified ex vivo, and then re-introduced into the affected individual--might be an effective strategy for treating human disease. (sciencedaily.com)
To test the effect of decreasing p53 to particular levels via RNA interference, the scientists reconstituted the blood cells of mice by first irradiating the animals to destroy their endogenous, bone marrow supply of hematopoietic stem cells, and then injected the mice with a fresh supply of hematopoietic stem cells that had been engineered through RNAi to produce low, medium, or high levels of p53. (sciencedaily.com)
The other two involve the ATM and ATR genes, which initiate a chain of events that stops cells from growing and dividing. (the-scientist.com)
Excellence Professor of Pathology and Laboratory Medicine at the University of North Carolina, Chapel Hill, are being honored for developing gene targeting - the ability to alter particular genes in cultured cells and transfer the targeted genes to laboratory mice. (utah.edu)
Cyclin A2f/+ ES cells were then injected into mouse blastocysts and homozygous cyclin A2f/f animals were generated using standard procedures (Geng et al. (immune-source.com)
In this important study, Drs. Thomas and Capecchi used both replacement and insertional vectors to introduce a neomycin resistance (neo r ) gene into exon 8 of the Hprt gene, showing that clones of transfected cells had lost HPRT function (becoming 6-TG resistant), and had gained Neo r activity. (ijbs.com)
In their paper, they concluded 'It is hoped that this combination of using ES cells as the recipient cell line and site-specific mutagenesis achieved by gene targeting will provide the means for generating mice of any desired genotype" [ 2 ]. (ijbs.com)
To solve this, Drs. Mansour, Thomas and Capecchi developed a strategy, called "Positive-Negative Selection" for enriching ES cells containing a targeted disruption of any transfected gene [ 8 ]. (ijbs.com)

However, a technical obstacle has to be overcome to achieve this goal because unlike Hprt , none of the other genes has a selective advantage after disruption. (ijbs.com)

This laboratory manual provides cutting-edge methods for generating and characterizing mouse models that accurately recapitulate many features of human cancer. (cshlpress.com)
Efforts to make a mouse model using traditional methods had been unsuccessful. (ubigene.us)
Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)

Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)

Dot1a binds Af9 and represses several aldosterone-upregulated genes including and promoter promotes H3 di-methyl K79 (H3m2K79) and inhibits transcription [24] [27]. (bioskinrevive.com)
The GFAP(2.2) promoter is based on human glial fibrillary acidic protein gene (GFAP), which is the major intermediate filament protein in astrocytes. (vectorbiolabs.com)

Cell death-inducing DFFA-like effector b, also known as CIDEB, is a human gene. (wikipedia.org)
Clinical and cell-based studies as well as investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological and pathophysiological contexts, underscoring their functional pleiotropy. (frontiersin.org)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
In mice with high B-cell p53 levels, lymphomas did not develop at an accelerated rate, and these mice did not experience decreased survival rates compared to control mice. (sciencedaily.com)
Q54831852 WW https://www.jax.org/jax-mice-and-services/find-and-order-jax-mice/biospecimens/deltagen-knockout-es-cell-lines CC Part of: Deltagen knockout ES cell lines. (cellosaurus.org)
1997 In the work explained below we decided to revisit the requirement for cyclin A function in cell proliferation using conditional cyclin A knockout mice. (immune-source.com)
Analyses of Cyclin A-null Fibroblasts We next derived fibroblasts from conditional cyclin A2 knockout embryos and cultured them culture (Physique 2A) and normally re-entered the cell cycle from quiescence (Physique 2B). (immune-source.com)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)

We selected and genotyped 21 tagging single nucleotide polymorphisms (SNPs) in five MMR genes ( MLH1, MLH3, PMS2, MSH4 and MSH5 ) using the SNPstream 12-plex platform in a case-control study of 1,292 idiopathic infertility patients and 480 fertile controls in a Chinese population. (biomedcentral.com)

Knockout mice also have been used to study and model varieties of cancer, obesity, heart disease and other illnesses. (wral.com)
Jessica Fowler and colleagues made a mouse model lacking the immunoglobulin recombination enzyme, RAG-2. (biologists.com)
Rapid advances in gene editing and genetic technologies have revolutionised our ability to model human genetic disease and provided new hope for gene therapies. (biologists.com)
The laboratory mouse is an important model for addressing questions in cancer biology. (cshlpress.com)
This mouse model will serve as critical means to elucidate the molecular mechanism involved in SPG33 pathology and will also allow us to examine why the nerves degenerate in HSP. (hsp-info.de)
Here, we show that this is a pathogenic hypomorphic variant in a mouse model. (nature.com)
Investigations on an in vitro neuroinflammation model revealed that GAC is increased in primary mouse microglia following pro-inflammatory stimulation. (shengsci.com)
Here, we present a comprehensive neuroanatomical survey of a Chd7 haploinsufficient mouse model of CHARGE syndrome. (bvsalud.org)
The study, published in the February issue of Nature Genetics, focussed on the role of a tumor suppressor gene called p53 in a mouse model of lymphoma. (sciencedaily.com)
This new mouse model, named HSA-FKRPdel, has a normal life span but present a severe dystrophic process in the skeletal muscle. (mdaconference.org)
The enhanced efficacy and exquisite specificity of SELECTIV has broad utility in development of new mouse model systems and expands the use of AAV for gene delivery in vivo. (nature.com)
CRISPR/Cas9 screens, which have revolutionised human and model organism research, have not yet been implemented in malaria parasites due to the need for more complex CRISPR/Cas9 gene targeting vector libraries. (portlandpress.com)
Here we show that three different humanized mice strains are highly susceptible to VARV infection, establishing the first small animal model using VARV. (cdc.gov)

Mice lacking the epidermal growth factor receptor (EGFR) develop epithelial defects and a neurodegenerative disease and die within the first month of birth. (silverchair.com)

Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard. (taconic.com)
Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)

The scientists, led by Greg Hannon and his CSHL colleague, Scott Lowe, knew that completely deleting the p53 gene causes lymphomas to develop much sooner, and in a more aggressive, highly-invasive form, than lymphomas that develop when the p53 gene is present. (sciencedaily.com)
Gene targeting is now practiced routinely by thousands of scientists all over the world, enabling them to address the most complex and critical biological problems, including the causes and treatment of birth defects and many other disorders, such as cancer, diabetes, and atherosclerosis. (utah.edu)

Pu, Wang, and colleagues then used gene therapy to replace TAZ, injecting an engineered virus under the skin (in newborn mice) or intravenously (in older mice). (ubigene.us)

Fibroblasts To rule out the possible contribution from cyclin A1 we crossed cyclin A2f/f mice with cyclin A1-null animals (Liu et al. (immune-source.com)

The CIDE null mice show decreased lipogenesis. (wikipedia.org)
In addition, the CIDEB null mice also have improved insulin sensitivity and enhanced hepatic fatty acid oxidation and whole body metabolism. (wikipedia.org)

While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)

Homozygous hEGFR KI/KI mice are viable and live up to six months. (silverchair.com)

NSMCE2, a novel super-enhancer-regulated gene, is linked to poor prognosis and therapy resistance in breast cancer. (nih.gov)
There is no cure or specific treatment, but new research at Boston Children's Hospital suggests that gene therapy could prevent or reverse cardiac dysfunction. (ubigene.us)
TAZ gene therapy also prevented cardiac dysfunction and scarring when given to newborn mice, and reversed established cardiac dysfunction in older mice -- whether the mice had whole-body or heart-only TAZ deletions. (ubigene.us)
Simply scaling up the dose of gene therapy won't work: In large animals like us, large doses risk a dangerous inflammatory immune response. (ubigene.us)
Giving multiple doses of gene therapy won't work either. (ubigene.us)

Cranial imaging studies are challenging in CHARGE syndrome patients, but high-throughput magnetic resonance imaging (MRI) techniques in mouse models allow for the unbiased identification of neuroanatomical defects. (bvsalud.org)
However, these mice are growth retarded and show skin and hair defects similar to Egfr -/- mutants. (silverchair.com)

RESULTS: Trio exome sequencing has revealed a novel pathogenic compound heterozygous variant c.145A >T (p.Lys49Ter) and c.224_242del (p.Val85GlufsTer88) in exon 2 of the C12orf57 gene. (bvsalud.org)

Anatomy8

Organisms8

Diseases4

Chemicals and Drugs43

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes75

Disciplines and Occupations1

Information Science4

Health Care1

Homologous21

Transgenic Mice1

Meiotic recombination2

Conditional knockout mice2

Resistant to high fat diet induced2

C57BL3

Embryonic3

Genome5

Inducible4

Exons3

5000 genes2

Temporal expression of ge1

Germline1

Genetically1

Variants3

Genomic2

Deletion4

Tissues6

Mammalian1

Mutation7

Tissue6

Chromosome2

Homolog1

Recombinase2

Mutant mice3

Pathways3

Alleles3

Allele3

Mutations in the gene3

CDNA1

Generate1

Endogenous gene2

Humans5

Expression8

Efficiency2

Phenotype6

Phenotypes1

Pathway1

Cells15

Disruption1

Methods3

Chromatin1

Promoter2

Cell8

MSH41

Model13

Receptor1

Murine2

Scientists2

Colleagues1

Fibroblasts1

Null mice2

Analyses1

Viable1

Therapy5

Defects2

Exon1