• The MA5-15692 immunogen is purified recombinant fragment of human ALDH1A1 expressed in E. Coli. (thermofisher.com)
  • A human recombinant protein (amino acids T6-Q342) was used as the immunogen for the ALDH1A1 antibody. (nsjbio.com)
  • Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
  • This Review summarizes the more recent biochemical, cellular and mouse model studies that underscore the importance of the ER acetylation process in maintaining protein homeostasis and autophagy within the secretory pathway, and its impact on developmental and age-associated diseases. (biologists.com)
  • The elevated expression can be repressed by adenomatosis polyposis coli (APC) , a tumor suppressor protein related to APC/beta-catenin signaling pathway. (caslab.com)
  • The engineered strain converts glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase AOR and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. (brenda-enzymes.info)
  • Although attractive fermentation performances were reached, significant improvements are required to eliminate recombinant plasmids, antibiotic resistances and inducible promoters, and increase ethanol tolerance. (biomedcentral.com)
  • RA production by hRALDH2 is efficiently inhibited by acetaldehyde but not by ethanol itself. (xenbase.org)
  • We conclude that acetaldehyde is the teratogenic derivative of ethanol responsible for the reduction in RA signaling and induction of the developmental malformations characteristic of FASD. (xenbase.org)
  • Acetaldehyde phenocopies the malformations induced by ethanol. (xenbase.org)
  • For this reason, the low cost and high-efficiency propanol production with the help of biosynthetic pathways from microorganisms is one of the major challenges in microbial fuel cells. (mrforum.com)
  • In this report, the authors devised synthetic genomic strategies to introduce essential amino-acid biosynthetic pathways into mammalian cells. (elifesciences.org)
  • Altogether, this work was found to be of substantial interest as it provides pioneering evidence that mammalian systems may be permissive to the restoration of essential amino acid biosynthetic pathways and is thus anticipated to have a broad impact in the fields of synthetic biology, biotechnology and beyond. (elifesciences.org)
  • In an in vitro reaction setup with NER-proficient and NER-deficient xeroderma pigmentosum group A (XPA) cell extracts, NER reactions were observed in the presence of XPA recombinant proteins in acetaldehyde-treated plasmids. (bvsalud.org)
  • Comprehensive Search for Novel Circulating miRNAs and Axon Guidance Pathway Proteins Associated with Risk of End Stage Kidney Disease in Diabetes. (cdc.gov)
  • These miRNAs targeted proteins in the axon guidance pathway. (cdc.gov)
  • Some circulating miRNAs and axon guidance pathway proteins represent potential targets for new therapies to prevent and treat this condition. (cdc.gov)
  • Conversion of glycerol to 2,3-BDO occurs through the oxidative pathway, where glycerol is converted to dihydroxyacetone phosphate (DHAP) via glycerol-3-phosphate in the presence of electron acceptors (e.g. (biomedcentral.com)
  • In addition to the light- or H2O2-induced formation of FICZ, a number of other enzymatic pathways have been identified to convert Trp to FICZ via the precursor I3A. (wikipedia.org)
  • A growing number of studies support the appreciation that LAB can also significantly, positively and negatively, contribute to the sensorial profile of wine through many different enzymatic pathways. (frontiersin.org)
  • Here, we report a new strain with chromosomally integrated ethanologenic pathway under the control of a constitutive promoter, without recombinant plasmids and resistance genes. (biomedcentral.com)
  • Over the last 15 years I have developed a research pathway in auxin biosynthesis going back to my degree and PhD work at the Technical University of Munich where I studied the nitrilase pathway in maize auxin biosynthesis and maize tryptophan synthase complex. (brookes.ac.uk)
  • Based on this work I won a fellowship from the Korean Federation of Science and Technology Societies to investigate the subcellular localisation of maize auxin biosynthesis at Dankook University in Seoul which lead to a publication that showed for the first time that both steps of the TAA/YUC pathway of auxin biosynthesis can be ER-localised. (brookes.ac.uk)
  • Major genomic deletions in independent eukaryotic lineages have led to repeated ancestral loss of biosynthesis pathways for nine of the twenty canonical amino acids. (elifesciences.org)
  • Previous studies have highlighted that EAA biosynthesis tends to be more energetically costly, raising the possibility that these pathways were lost from organisms with access to abundant EAAs. (elifesciences.org)
  • 13 C-tracing verified de novo biosynthesis of valine and further revealed build-up of pathway intermediate 2,3-dihydroxy-3-isovalerate. (elifesciences.org)
  • Whole genome sequencing across the tree of life has revealed the surprising observation that nine essential amino acid (EAA) biosynthesis pathways are missing from the metazoan lineage ( Payne and Loomis, 2006 ). (elifesciences.org)
  • Acetaldehyde inhibits retinoic acid biosynthesis to mediate alcohol teratogenicity. (xenbase.org)
  • Ergothioneine production by Corynebacterium glutamicum harboring heterologous biosynthesis pathways. (hokudai.ac.jp)
  • Previously, we found that acetaldehyde induces reversible intra-strand GG crosslinks in DNA similar to those induced by cis-diammineplatinum(II) that is subsequently repaired by NER. (bvsalud.org)
  • These findings show that acetaldehyde induces NER repairable mutagenic DNA lesions. (bvsalud.org)
  • Acetaldehyde induces a reduction in retinoic acid signaling. (xenbase.org)
  • Although FICZ itself has not been convincingly identified in the mouse colon, the precursors of FICZ e.g. indole-3-pyruvate, indole-3-acetaldehyde (I3A), and tryptamine, have been found. (wikipedia.org)
  • The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. (brenda-enzymes.info)
  • Pyruvate is first converted into acetaldehyde by pyruvate decarboxylase. (jove.com)
  • In the absence of oxygen, the pyruvate molecules are then converted into different organic molecules through a variety of pathways depending on the organism involved. (jove.com)
  • In the first analyses of the metabolism of FICZ, human recombinant CYP1A1 enzyme and S9 fractions prepared from rat liver and mouse Hepa-1 cells were used. (wikipedia.org)
  • for example, recombinant viruses can be genetically engineered to express immunomodulatory genes or tumor-specific antigens to stimulate an anti-cancer immune response. (mdpi.com)
  • Purified recombinant fragment of human ALDH1A1 expressed in E. Coli. (thermofisher.com)
  • Kinetic studies using human recombinant CYP1A1, -1A2, and -1B1 expressed in E. coli showed that the catalytic efficiencies (specificity constant kcat/Km) were 5-50-fold higher for FICZ than for the standard substrates 7-ethoxyresorufin and 7-metoxyresorufin and at least 5000-fold higher compared with the hydroxylation of estrogens. (wikipedia.org)
  • We provide biochemical and developmental evidence identifying acetaldehyde as responsible for this inhibition. (xenbase.org)
  • Enzymatic kinetic analysis of human RALDH2 shows a preference for acetaldehyde as a substrate over retinaldehyde. (xenbase.org)
  • One synthetic pathway produced valine at a sufficient level for cell viability and proliferation. (elifesciences.org)
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • The data revealed GZFL obviously suppressed the proliferation and triggered the apoptosis of acetaldehyde-stimulated LX-2 cells. (bvsalud.org)
  • Recombinant viruses are a novel therapeutic tool that can be utilized in the treatment of various diseases, including cancer. (mdpi.com)
  • The genome-based cg3035 modulated strain was further engineered by attenuating the expression of pta and cat , inserting a strong P eftu promoter in the upstream region of glycolytic enzymes such as pfkA , gap , and pyk , and redirecting carbon flux to the pentose phosphate pathway. (biomedcentral.com)
  • The recombinant strain produces 0.182 g/l of 3-methyl-1-butanol and 0.144 g/l of isobutanol after 12 h of incubation. (brenda-enzymes.info)
  • The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. (nih.gov)
  • There exists sufficient evidence against acetaldehyde suggesting it to cause a variety of DNA lesions and be carcinogenic to humans. (bvsalud.org)
  • Another approach is recombinant oncolytic viruses that can replicate specifically in tumor cells and induce toxic effects leading to cell lysis and apoptosis. (mdpi.com)
  • Additionally, a minimally or non-acidic gastric environment contains an altered microbiome that can increase the production of acetaldehyde and N-nitrosamines. (bvsalud.org)
  • The mechanistic data for all types of can be used to identify and organize fects (e.g. is able to induce genotox- ionizing radiation ( IARC, 2012f) are mechanistic information related to icity or affect similar pathways), but particularly informative with regard to cancer induction. (who.int)
  • In addition to the oxidative branch, glycerol is also metabolized through the reductive pathway, which results in the generation of 1,3-PDO (Fig. 1 ). (biomedcentral.com)
  • transcriptional profiling for aspergillusnidulans hoga mapk signaling pathway in response to fludioxonil and osmotic stress. (liverpool.ac.uk)
  • Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Translocation of GLUT4 to the plasma membrane. (caslab.com)
  • In addition, GZFL prevented acetaldehyde-induced activation of LX-2 cells via downregulation of TGF-ß1, p-Smad2, p-Smad3, CUGBP1, and upregulation of p-STAT1 and Smad7. (bvsalud.org)
  • Increasing the dosage of downstream ilvD boosted pathway performance and allowed for long-term propagation of second-generation cells in valine-free medium at 3.2 days per doubling. (elifesciences.org)
  • CRISPR-Cas9 inactivation involving TaPsIPK1 in grain confers broad-spectrum resistant against Pst without having influencing important agronomic traits in 2 numerous years of field Anti-CD22 recombinant immunotoxin tests. (plasignaling.com)