• The 332 amino acid recombinant protein has a predicted molecular mass of approximately 36.7 kD. (biolegend.com)
  • 1. Dilute the recombinant mouse Cathepsin B to 10 μg/ml in activation buffer (25 mM MES, 1 mM DTT, pH 5.0) and incubate the protein at 25°C for 3 min. (biolegend.com)
  • Cathepsin D is a protein that in humans is encoded by the CTSD gene. (wikipedia.org)
  • A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human TPP1. (novusbio.com)
  • The yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein. (iyte.edu.tr)
  • We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
  • Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
  • Cathepsin E Protein, Human (HEK293, His) is an approximately 46.0 kDa mouse cathepsin Dwith a His tag. (medchemexpress.com)
  • Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1 17), a propeptide (residues 18 53), and a mature chain (residues 54 396) [3] . (medchemexpress.com)
  • The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet. (medchemexpress.com)
  • The main function of cathepsin D is to degrade proteins and activate precursors of bioactive proteins in pre-lysosomal compartments. (wikipedia.org)
  • The main physiological functions of cathepsin D consist of metabolic degradation of intracellular proteins, activation and degradation of polypeptide hormones and growth factors, activation of enzymatic precursors, processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death. (wikipedia.org)
  • Cathepsin E functions by breaking down proteins through the hydrolysis of peptide bonds at a specific peptide sequence site. (medchemexpress.com)
  • And Cathepsin E plays an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing [2] . (medchemexpress.com)
  • Explore our comprehensive collection of recombinant Fc receptor proteins! (acrobiosystems.com)
  • This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85561. (novusbio.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Cathepsin K (CTSK) in serum, plasma, tissue homogenates and other biological fluids. (srbiosystem.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Cathepsin K (CTSK) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (srbiosystem.com)
  • Cathepsin B, also known as CTSB, is a lysosomal cysteine protease. (biolegend.com)
  • While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. (biolegend.com)
  • Lysosomal cathepsin B (CTSB) has been proposed to play a role in the induction of acute inflammation. (frontiersin.org)
  • These observations suggest that during Mtb infection, lysosomal release of activated CTSB and possibly other cathepsins inhibitable by CA07-Me is critical for the induction of inflammasome-mediated IL-1β processing by regulating NLRP3-inflammasome assembly in the cytosol. (frontiersin.org)
  • CTSB Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 330 amino acids (18-339a.a.) and having a molecular mass of 36.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). (lifetein.com)
  • Cathepsin B, Cathepsin B1, CTSB. (lifetein.com)
  • CTS protease activity also measured by zymograph electrophoresis of Cathepsins. (histoready.com)
  • Cathepsin D is an aspartic endo-protease that is ubiquitously distributed in lysosomes. (wikipedia.org)
  • Cathepsin-D is an aspartic protease that depends critically on protonation of its active site Asp residue. (wikipedia.org)
  • Along with Asp-protonation, lower pH also leads to conformational switch in cathepsin-D : the N-terminal segment of the protease moves out of the active site as pH drops. (wikipedia.org)
  • Cathepsin E is an aspartic protease and a member of the peptidase A1 family of proteases. (medchemexpress.com)
  • As an intracellular, hydrolytic aspartic protease, Cathepsin E is mainly expressed in cells of the immune and gastrointestinal systems, lymphoid tissues, erythrocytes, and cancer cells [1] . (medchemexpress.com)
  • A serine protease which inactivates nucleases Biomolecule/Target: Proteinase K, Recombinant, NGS Grade Alternates names: Protease K, Endopeptidase K, Tritirachium. (telospub.com)
  • Moreover, cathepsin inhibition abolished the interaction between NLRP3 and ASC, measured by immunofluorescence imaging in H37Rv-infected macrophages, demonstrating a critical role of the enzyme in NLRP3-inflammasome activation. (frontiersin.org)
  • Little is known, however, about the mechanism by which the propeptide of FhCL3, a cathepsin L peptidase secreted by the infective newly excysted juveniles (NEJs), regulates the inhibition and activation of the mature enzyme before it is secreted into host tissues. (biomedcentral.com)
  • Using recombinant variants of ppFhCL3 we demonstrated the critical importance of a pair of propeptide residues (Tyr 46 Lys 47 ) for the interaction with the propeptide binding loop (PBL) of the mature enzyme and other residues (Leu 66 and Glu 68 ) that allow the propeptide to block the active site. (biomedcentral.com)
  • Our enzyme kinetics data demonstrates high potency and selectivity of the ppFhCL3 for its cognate FhCL3 enzyme, information that could be utilised to design inhibitors of parasite cathepsin L peptidases. (biomedcentral.com)
  • Journal Article] Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type. (nii.ac.jp)
  • Among the most potent novel inhibitors, one peptide, RKLLW-NH2, shares the amphiphilic character of the nonamer fragment VMNGLQNRK of the autoinhibitory, substrate-like, but reverse-binding prosegment of human cathepsin L which blocks the active center of the enzyme. (uni-bielefeld.de)
  • Cathepsin L (Cat L) enzyme, found in all stages of the liver flukes, plays a significant role in the biological functions of the parasites, such as migration, nutrition, and immune evasion ( 2 ). (brieflands.com)
  • The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. (eurogentec.com)
  • The recombinant human enzyme is catalytically active at pH 6.0 and is inhibited by compound E-64. (qmul.ac.uk)
  • Whereas the other cysteine Cathepsins (e.g. (justia.com)
  • Cathepsin S is exclusive amongst mammalian cysteine cathepsins in being lively and steady at impartial pH. (teitell-lab.com)
  • We determined that FhCyLS-2 is a broad specificity inhibitor of cysteine cathepsins from both the host and F. hepatica, suggesting a dual role in the regulation of exogenous and endogenous proteolysis. (bvsalud.org)
  • The activity of Cathepsin B is determined by its ability to cleave the fluorogenic peptide substrate, Z-Leu-Arg-AMC (Z=Benzyloxycarbonyl, and AMC=7-amino-4-methylcoumarin) after activation. (biolegend.com)
  • Analysis of the culture supernatant revealed that the recombinant human CatC was secreted as a 58-kDa molecule, indicating that human CatC was accumulated in the culture supernatant as proform composed of the residual propart, the activation peptide, and the heavy and light chains. (iyte.edu.tr)
  • Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. (cusabio.com)
  • This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm. (eurogentec.com)
  • This invention relates to a compound of formula I and their use as inhibitors of Cathepsin C, pharmaceutical compositions containing the same, and methods of using the same as agents for treatment and/or prevention of diseases connected with dipeptidyl peptidase I activity, e.g. respiratory diseases. (justia.com)
  • Thus, inhibitors of Cathepsin C could potentially be useful therapeutics for the treatment of neutrophil-dominated inflammatory diseases such as chronic obstructive pulmonary disease (COPD), pulmonary emphysema, asthma, multiple sclerosis, and cystic fibrosis (Guay et al. (justia.com)
  • By screening a combinatorial pentapeptide amide collection in an inhibition assay, we systematically evaluated the potential of 19 proteinogenic amino acids and seven nonproteinogenic amino acids to serve as building blocks for inhibitors of human cathepsin L. Particularly efficient were aromatic, bulky, hydrophobic amino-acid residues, especially leucine, and positively charged residues, especially arginine. (uni-bielefeld.de)
  • This random approach for the design of inhibitors was introduced to compensate for the inaccuracy induced by shifted docking of combinatorial compound collections at the active center of cathepsin L. Thereby, we obtained structurally defined pentapeptide amides which inhibited human cathepsin L at nanomolar concentrations. (uni-bielefeld.de)
  • Highly potent inhibitors of human cathepsin L identified by screening combinatorial pentapeptide amide collections", EUROPEAN JOURNAL OF BIOCHEMISTRY , vol. 267, 2000, pp. 5085-5092. (uni-bielefeld.de)
  • The association rate constants for the binding of recombinant human cystatin C to papain, ficin, actinidin and recombinant rat cathepsin B were 1.1 x 10(7), 7.0 x 10(6), 2.4 x 10(6) and 1.4 x 10(6) M-1.s-1, whereas the corresponding dissociation rate constants were 1.3 x 10(-7), 9.2 x 10(-6), 4.6 x 10(-2) and 3.5 x 10(-4) s-1. (lu.se)
  • Part of the parasite's wide-ranging virulence is attributed to the expression and secretion of large amounts of papain-like cysteine peptidases, namely cathepsin L and cathepsin B [ 3 ]. (biomedcentral.com)
  • Extracellular recombinant proCatC was further activated by cysteine endoprotease papain in vitro and its activity was confirmed by assays using a synthetic substrate. (iyte.edu.tr)
  • Cathepsin O is a lysosomal cysteine peptidase of family C1 (papain family). (qmul.ac.uk)
  • The zoonotic worm parasite Fasciola hepatica secretes an abundance of cathepsin L peptidases that are associated with virulence, invasiveness, feeding and migration. (biomedcentral.com)
  • Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics? (brieflands.com)
  • Description: A sandwich ELISA kit for detection of Cathepsin K from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (srbiosystem.com)
  • It has been surprisingly found that the bicyclic compounds of the present invention possess potent Cathepsin C activity, high selectivity against other Cathepsins, e.g. (justia.com)
  • These values are at least 1000-fold lower than those K i obtained for human cathepsin L (HsCL) and human cathepsin K (HsCK) demonstrating the selectivity of the ppFhCL3 for parasite cathepsins L. By exploiting 3-D structural data we identified key molecular interactions in the specific binding between the ppFhCL3 and FhCL3 mature domain. (biomedcentral.com)
  • Recombinant Human Cathepsin B is produced by our Mammalian expression system and the target gene encoding Arg18-Ile339 is expressed with a 6His tag at the C-terminus. (bonopusbio.com)
  • Knock-out of CTSD gene would cause intestinal necrosis and hemorrhage and increase apoptosis in thymus, indicating that cathepsin D is required in certain epithelial cells for tissue remodeling and renewal. (wikipedia.org)
  • Entrez Gene: CTSD cathepsin D". Barrett AJ (April 1970). (wikipedia.org)
  • 1. Santamaría, I., Pendás, A.M., and López-Otín, C. Genomic structure and chromosomal localization of a human cathepsin O gene (CTSO). (qmul.ac.uk)
  • After 15 days, we performed osteoclasts identification through tartrate resistant acid phosphatase (TRAP) staining and osteoclasts activity assessment through TRAF6, NFATC1 and Cathepsin K gene expression. (unina.it)
  • Neutrophils are recruited to the site of joint inflammation and release Cathepsin G, elastase and proteinase 3, proteases which are believed to be responsible for cartilage destruction associated with rheumatoid arthritis. (justia.com)
  • Proteinase K, Recombinant, NGS Grade is available at Gentaur for Next week delivery. (telospub.com)
  • Cathepsin D can also be found in the extracellular space and it is one of the few cathepsins, that shows some activity at neutral pH. (wikipedia.org)
  • Cloning and sequence analysis of cDNA for human cathepsin D". Proceedings of the National Academy of Sciences of the United States of America. (wikipedia.org)
  • Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G. (rndsystems.com)
  • The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. (rndsystems.com)
  • Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans. (teitell-lab.com)
  • ATP1B2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (68-246) and having a molecular mass of 27.8kDa. (neuromics.com)
  • Pharmacological inhibition of cathepsin activity with CA074-Me resulted in a substantial reduction of both mature IL-1β production and caspase-1 activation in infected macrophages. (frontiersin.org)
  • Cathepsin D enzymatic activity induces hydrolytic modification of apolipoprotein B-100-containing lipoproteins, including LDL, which means it may be involved in atherosclerosis as well. (wikipedia.org)
  • Mouse Cathepsin B, amino acids (His18-Phe339) (Accession# P10605), with C-terminal 10x His tag, was expressed in CHO cells. (biolegend.com)
  • Add 50 μl of the diluted, activated Cathepsin B (0.0025 µg/well) to a black well and start the reaction by adding 50 μl of 40 μM substrate. (biolegend.com)
  • After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3). (rndsystems.com)
  • The catalytic sites of cathepsin D include two critical aspartic residues (amino acid 33 and 231) located on the 14 kDa and 34kDa chains. (wikipedia.org)
  • Over-expression of cathepsin D stimulates tumorigenicity and metastasis as well as initiation of tumor apoptosis. (wikipedia.org)
  • High specific activity, heat-labile alkaline phosphatase purified from a recombinant source. (telospub.com)
  • Similar to other aspartic proteases, cathepsin D accommodates up to 8 amino acid residues in the binding cleft of the active site. (wikipedia.org)
  • Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. (eurogentec.com)
  • Shrimp Alkaline Phosphatase, Recombinant is available at Gentaur for Next week delivery. (telospub.com)
  • Our results showed that pro-osteoclastogenesis mediators RANKL, IL-6, IL-33, OPN and TNF-α were overexpressed in ex vivo skin organ cultures stimulated with recombinant IL-33, OPN and TNF-α in a time, but not dose, dependent manner. (unina.it)
  • The potential of recombinant ALP to block the interactions of leukocytes with the endothelial lining was concomitantly investigated in vitro and in vivo . (biomedcentral.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Rat Cathepsin K (CTSK) in samples from serum, plasma, tissue homogenates or other biological fluids. (srbiosystem.com)
  • The ultimate form of mature cathepsin D is composed of 337 amino acid residues, 196 amino acid residues in the heavy chain and 141 in the light chain. (wikipedia.org)
  • Similarly the activation markers of mature osteoclasts, TRAF6, NFATC1 and Cathepsin K, were enhanced. (unina.it)
  • Explore our series of recombinant neural factors to support the culture and differentiation of nerve cells. (acrobiosystems.com)
  • Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp. (uni-bielefeld.de)