Recognition genes dna copy number variations. Medical search. Frequent questions

The concept of the replicator as a genetic entity has proven very useful in the quest to identify replicator DNA sequences and initiator proteins in prokaryotes, and to some extent also in eukaryotes, although the organization and complexity of replicators differ considerably between the domains of life. (wikipedia.org)
This uses fluorescent-labelled DNA probes complementary to specific sequences of interest, such as those from within regions commonly deleted or duplicated in particular syndromic disorders. (dnalabsindia.com)
Multiplex ligation-dependent probe amplification (MLPA) is an application of the polymerase chain reaction (PCR) in which it is not the patient's DNA that is amplified by PCR but, instead, carefully designed probe sequences. (dnalabsindia.com)
The two probes carry an additional sequence, not complementary to human DNA sequences, including PCR primer binding sites and additional 'stuffer sequences' designed so that the PCR product is of a very specific size. (dnalabsindia.com)
Single-stranded sequences of DNA from across the genome (or from specific areas of the genome of particular interest) are laid out in a structured array of microdots. (dnalabsindia.com)
The length of these sequences has become shorter with each new generation of the technology: originally consisting of bacterial artificial chromosomes (BACs) with many kilobases of human DNA inserted, they now usually consist of oligonucleotides (oligos) of some 20+ bases. (dnalabsindia.com)
In addition, 85 DNA segments were identified, including some genic sequences. (biomedcentral.com)
The length of these repeating sequences , at one time dismissed as mere junk DNA, in the gene that codes for a key hormone receptor determined male-female relations and parenting behaviors in a species of voles. (news-medical.net)
Hammock and Young were particularly intrigued with microsatellites, repeating sequences of letters in the genetic code peppered throughout these regulatory areas of the vasopressin receptor gene. (news-medical.net)
for example, the repeating letter sequences are much longer in monogamous than in polygamous vole species, and even within a species, there are differences in the number of letters in the sequence among individuals. (news-medical.net)
According to Young, far from being junk, the repetitive DNA sequences, which are highly prone to mutate rapidly, may ultimately exert their influence through complex interactions with other genes to produce individual differences and social diversity. (news-medical.net)
The pluripotency of the initial cell and the establishment of cell types depend to a large extent on the coordinated deployment of hundreds of transcription factors that bind to specific DNA sequences to activate or repress the transcription of cell lineage genes 1 . (nature.com)
We have adapted this fundamental strategy to develop a high-throughput practical assay for the recognition of active CRMs named FIREWACh (enhancer DNA sequences which are specifically active in ESCs15 upstream of the minimal promoter within FpG5. (californiaehealth.org)
Therefore the combined use of two enzymes with Isoforskolin unique recognition sequences raises genomic protection and better captures the diversity of regulatory elements within ESC chromatin. (californiaehealth.org)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
Hidden Markov Models (HMM) are often used for analyzing Comparative Genomic Hybridization (CGH) data to identify chromosomal aberrations or copy number variations by segmenting observation sequences. (biomedcentral.com)
Continuous observation sequences from either DNA microarrays or next generation sequencing experiments, note that the proportion of mapped reads in an interval is frequently used as a continuous measure of copy number, to detect chromosomal aberrations or copy number variations lead to the same fundamental computational problem and share characteristics of the data. (biomedcentral.com)

For epigenetic analyses, the researchers used a DNA methylation microarray that evaluated over 0.85 million 5'-cytosine-phosphate-guanine-3' (CpG) sites. (news-medical.net)
They calculated the absolute age differences for 16 look-alike pairs according to their chronological and epigenetic age, i.e., per their date of birth and DNA methylation clock, respectively. (news-medical.net)
In general, the results revealed that These individuals share similar genotypes , but differ in the methylation (epigenetic modification) of DNA and in the microbiome landscape. (wireservice.ca)
For example, a DNA methyltransferase gene in the wheat pathogen Zymoseptoria tritici (synonym Mycosphaerella graminicola ) was amplified to tens of copies, all of which were inactivated by Repeat-Induced Point mutation (RIP) including the original, resulting in loss of cytosine methylation. (biomedcentral.com)
Droplet digital PCR (ddPCR) allows absolute quantification of nucleic acids and has potential for improved non-invasive detection of DNA methylation. (biomedcentral.com)
Implementation of a robust internal control, i.e., the 4Plex, and an algorithm for automated threshold determination, PoDCall, in methylation-specific ddPCR increase the precision of DNA methylation analysis. (biomedcentral.com)
The aims of the present study were to develop a robust internal control for ddPCR DNA methylation analyses and demonstrate its value in terms of increased precision of the normalized methylation data. (biomedcentral.com)
DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)
129 genomic copies/L for CpG methylation capture with MDA. (cdc.gov)

Incomplete, erroneous, or untimely DNA replication events can give rise to mutations, chromosomal polyploidy or aneuploidy, and gene copy number variations, each of which in turn can lead to diseases, including cancer. (wikipedia.org)
Family history remains one of the major risk factors that contribute to cancer, and recent studies have identified several genes whose germline mutations are associated with cancer. (nature.com)
8 Genetic tests based on these highly penetrant gene mutations have shown their usefulness, but they can explain only a small fraction (5-10%) of patients. (nature.com)
Mutations in the genes BRCA1, BRCA2 or PIK3CA have important implications in the cellular DNA repair mechanisms, and are associated with sensitivity and improved response to platinum-based chemotherapy and PAPR inhibitors. (unilabs.ch)
Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. (cancerindex.org)
Basically we have to take into account that Expression of genes (Any number of copies Rna which produces a cell of a specific gene) that determines tissue growth, and It is very likely that people with the same mutations, or SNPs, may have a similar oval face shape, or a similar nose. (wireservice.ca)
After reminding the reader of the basic concepts of mitochondrial genetics, she offers examples of disorders due to mutations in mitochondrial DNA and mutations in nuclear DNA. (medlink.com)
This includes some cell lines contaminated with bacteria or carrying genes and mutations associated with cancer. (neurosciencenews.com)

To ensure complete and accurate duplication of the entire genome and the correct flow of genetic information to progeny cells, all DNA replication events are not only tightly regulated with cell cycle cues but are also coordinated with other cellular events such as transcription and DNA repair. (wikipedia.org)
The PCR reaction of the probe sequence, not the human sequence, can then proceed, yielding a quantity of product that relates directly to the number of copies of the target DNA in the patient's genome. (dnalabsindia.com)
The B sub-genome (1620) showed more number of markers than the A sub-genome (1337). (biomedcentral.com)
High resolution trait mapping in peanut ( Arachis hypogaea L. 2n = 4x = 40) demands a considerably large number of evenly distributed genome-wide markers to identify marker-trait associations. (biomedcentral.com)
But, analysis of whole genome re-sequencing (WGRS) data from a large number of diverse genotypes is expected to capture all AIPs when the short reads are analyzed using the computational method polymorphic TEs and their movement detection (PTEMD) [ 23 ] for the de novo discovery of AIPs. (biomedcentral.com)
Authors are encouraged to attempt these aneuploid analyses when reporting scRNA-seq data, much like copy-number variants are commonly reported in bulk genome sequencing data. (techscience.com)
These findings will have future implications in forensics, in reconstructing a criminal's face from DNA, and in genetic diagnosis: an image of a patient's face will already provide clues to the genome he has, Esteller says. (wireservice.ca)
Illumina sequencing exposed a total of 84 240 elements in the two NFR-DNA libraries that were found to be normally 154 bp in length and to align with unique positions in the mouse research genome. (californiaehealth.org)
In contrast random DNA fragments with a similar size distribution generated by digestion Isoforskolin of the mouse genome displayed only poor correspondence with DNaseI-accessible areas as expected (AUROC=0.52 Fig. 2a and Supplementary Number 1). (californiaehealth.org)
Number 2 NFR-derived DNAs correspond to accessible chromatin areas located throughout the genome Separate analysis of the HaeIII and RsaI NFR DNAs showed that both NFR populations displayed comparable positioning with DNaseI-accessible sites but the genomic areas targeted by each enzyme were largely unique and non-overlapping (Fig. 2b). (californiaehealth.org)
Transposable elements (TEs) can be key drivers of evolution, but the mechanisms and scope of how they impact gene and genome function are largely unknown. (biomedcentral.com)
To further test the effects of transposon-aided gene amplifications on genome evolution and architecture, the repetitive fraction of the significantly expanded genome of the banana pathogen, Pseudocercospora fijiensis , was analyzed in greater detail. (biomedcentral.com)
These results indicate that the interplay of TEs and RIP can result in different and unpredictable fates of amplified genes, with variable effects on gene and genome evolution. (biomedcentral.com)
TEs have been detected in the genomes of both prokaryotic and eukaryotic organisms [ 1 ], and have been rightly labeled as 'drivers of genome evolution' [ 2 ] due to their direct and indirect impacts on genes and genomes. (biomedcentral.com)
For example, Alu elements, at more than a million copies comprising 11% of the human genome, are a major contributor to primate genome evolution and the standing genetic diversity in human populations [ 3 ]. (biomedcentral.com)
Indeed, a number of genes identified as potential manipulators of the host cell phenotype are absent from the T . equi genome. (biomedcentral.com)
During cell division processes, genome duplication involves DNA dosage increase at discrete times in phase, and introduces considerable variations in gene copies [13C15]. (cckn-ia.org)
Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing. (cdc.gov)
SWGA is a robust method that allows direct whole-genome sequencing (WGS) of specimens containing very low numbers of T. pallidum, which has been challenging until now. (cdc.gov)

DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
DEAF-1 recognizes several TTCG motifs within the portion of the Dfd autoregulatory region termed 'module E.' In addition, DEAF-1 binds several similar motifs within a Dfd response element ( DRE ) from the 1.28 gene that enhances maxillary gene expression during embryogenesis (Pederson, 2000). (sdbonline.org)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
The results were validated both by gene expression analysis and immunohistochemistry study in an independent 55 clinical samples from Fudan University Shanghai Cancer Center. (cancerindex.org)
Copy number variations (CNVs) can create new genes, change gene dosage, reshape gene structures, and modify elements regulating gene expression. (biomedcentral.com)
As with all types of genetic variation, CNVs may influence phenotypic variation and gene expression. (biomedcentral.com)
Thus, CNVs are considered likely major sources of genetic variation, and may influence phenotypic variation and gene expression. (biomedcentral.com)
CNVs also have an impact on fitness and gene expression. (biomedcentral.com)
Clinical information, follow-up information, and gene expression profiles were obtained from the TCGA and GEO databases. (frontiersin.org)
Weighted gene co-expression network analysis (WGCNA) was used to identify key genes for mRNA vaccines. (frontiersin.org)
Three immune subtypes were classified based on immune-related gene expression profiles. (frontiersin.org)
The researchers first demonstrated in cell cultures that the vole vasopressin receptor microsatellites could modify gene expression. (news-medical.net)
Cellular identity is now accepted to be the result of the expression of specific combinations of genes (Fig. 1 ). (nature.com)
Finally, higher-order 3D chromosome folding is also thought to modulate gene expression and might contribute to inheritance 15 . (nature.com)
The deconvolution of cellular components is the reverse process of the convolution of cell subtypes in body tissues based on gene expression characteristics. (medicaltrend.org)
Some major effects of Alu -element amplifications include alterations of gene expression from insertions near gene promotors, insertional mutagenesis and repeat-mediated non-homologous recombination that can lead to disease, and 'exonization' of Alu elements yielding alternative splicing of transcripts. (biomedcentral.com)
We describe aspects of phero-perception, stress responses, immune function and regulation of gene expression that support the persistence of B. glabrata in the field and may define this species as a suitable snail host for S. mansoni. (westminster.ac.uk)
They can also interact with nucleic acids, like DNA and RNA, regulating gene expression. (atlasbars.com)
In budding yeast, acetylation of histone 3 suppresses transcription activity to buffer changes in DNA dose for expression homeostasis of other genes during DNA replication [13]. (cckn-ia.org)
Cell division cycle has global effects on mRNA and protein synthesis, and is also an important source of gene expression noise [10C13]. (cckn-ia.org)
Numerous cellular proteins detect DNA damage and induce senescence , a permanent change of state characterized by morphological and gene expression changes. (massgenomics.org)

Once bound to replicators, initiators (often with the help of co-loader proteins) deposit replicative helicases onto DNA, which subsequently drive the recruitment of additional replisome components and the assembly of the entire replication machinery. (wikipedia.org)
Epigenetic components (for example, Polycomb PRC1/2 and Trithorax group proteins) maintain the 'off' states of certain genes and the 'on' states of others, in a cell-type- and time-specific manner (the bottom panels show three genes, depicted schematically as chromatinized templates, in which transcription is triggered by specific transcription factors and silent or active states are maintained by PRC1/2 or Trithorax proteins, respectively). (nature.com)
Following the isolation of trypsinogen and chymotrypsinogen as specific proteins, DNase 1 was isolated in crystalline form from bovine pancreas and shown to be a heat sensitive protein that could cleave thymus DNA (16). (pancreapedia.org)
TE-rich genomic islands in expanded fungal ( P. fijiensis , L. maculans ) and oomycete ( Phytophthora infestans ) genomes carry genes that code for lineage-specific, putative small, secreted proteins. (biomedcentral.com)
Proteins interact with other molecules in a number of ways. (atlasbars.com)
Furthermore, proteins can also interact with carbohydrates, which are important for cell signaling and recognition. (atlasbars.com)

Somatic mutation and DNA copy number variation of BLCA were visualized by cBioPortal. (frontiersin.org)

Genomic analyses clustered only one couple together per copy number variation (CNVs). (news-medical.net)
Several approaches have been developed to improve our recognition of such copy number variants (CNVs). (dnalabsindia.com)
There is extensive variation in CNVs among healthy individuals, although finding a variant in a healthy individual does not prove that the CNV has no pathogenic significance: penetrance of the disease phenotype may be incomplete and interactions with environmental factors, other CNVs or other genetic variants may be required for its pathological effects to manifest. (dnalabsindia.com)
CNVs are thus considered major sources of genetic variation. (biomedcentral.com)
These CNVs contain known genes. (biomedcentral.com)
Further discussion of CNVs is important, as they are linked to variation among rice varieties, and are likely to contribute to subspecific characteristics. (biomedcentral.com)
Copy number variations (CNVs), or copy number polymorphisms (CNPs), are forms of structural variation (SV) that are alterations in DNA resulting in the cell having an abnormal number of copies of one or more segments of DNA. (biomedcentral.com)

A key prerequisite for DNA replication is that it must occur with extremely high fidelity and efficiency exactly once per cell cycle to prevent the accumulation of genetic alterations with potentially deleterious consequences for cell survival and organismal viability. (wikipedia.org)
5 Two distinct types of multiple endocrine neoplasias are associated with the RET and MEN1 6 genes while VHL alterations result in kidney and other types of cancer. (nature.com)
When neoplasms arise, they are modulated by the interactions of multiple genes based on a great diversity of genetic alterations, which leads to high tumoral heterogeneity. (nature.com)
With the increasing implementation of NGS screening in clinical oncology, the number of actionable genomic alterations has expanded in recent years. (unilabs.ch)
Whereas over 900 tools are available to analyze scRNA-seq data (as monitored on https://www.scrna-tools.org/), only a handful output aneuploidy data or its sub-chromosomal equivalent copy number alterations (CNAs). (techscience.com)

Furthermore, they evaluated the face gene enrichment in 19,277 SNPs of 3,730 genes by applying a hypergeometric test and a 10,000 iterations Monte Carlo simulation. (news-medical.net)

Two control design approaches were tested: (a) targeting a genomic region shared across members of a gene family and (b) combining multiple assays targeting different pericentromeric loci on different chromosomes. (biomedcentral.com)

We have shown that colorectal cancer cell lines defective in DNA MMR exhibit an increased sensitivity to both camptothecin, a topoisomerase I inhibitor, and etoposide, a topoisomerase II inhibitor. (aacrjournals.org)

Array comparative genomic hybridization in patient one and patient two revealed copy-number variant (CNV) deletions, respectively, ~ 1.45 Mb in size involving FOXF1 and an ~ 0.7 Mb in size involving FOXF1 enhancer and leaving FOXF1 intact. (researchgate.net)
Indeed, if hybridization intensity was an exact, un-biased measurement of DNA concentration before amplification, the sequence of hybridization intensities of probes along a chromosome would yield a piece-wise constant function in ArrayCGH experiments. (biomedcentral.com)
In recent years, many real-time monitoring methods, such as single molecule fluorescent in situ hybridization (smFISH), have been developed to estimate mRNA copy numbers in different cell cycle stages. (cckn-ia.org)

In addition, the inherited germline variants from these gene signatures were predominately enriched in T cell function, antigen presentation, and cytokine interactions, likely impairing the adaptive and innate immune response thus favoring a pro-tumorigenic environment. (nature.com)
Finally, 13 key immune genes were identified. (frontiersin.org)
However, given that only a small number of patients respond to immune checkpoint blockade and other immunotherapy strategies, more new technologies are needed to decipher the tumor cells and tumor immune microenvironment ( TME). (medicaltrend.org)
A common feature of GSEA-based methods is the need to establish a specific gene set for each immune cell subpopulation of interest. (medicaltrend.org)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
They are large, complex molecules that play a vital role in a number of biological functions including digestion, muscle contraction, and immune response. (atlasbars.com)
Recent advances have been made in the of host factors, polymorphisms, and candidate genes associated characterization of the immune response to low-molecular- with occupational asthma may improve our understanding of weight agents. (cdc.gov)

It was found that the ratio of the average number of mRNA copies in phase and are degraded almost completely before cytokinesis [12]. (cckn-ia.org)
From the measurements of [12], we estimated that the median of 21-Deacetoxy Deflazacort cytoplasmic CLB2 mRNA copy numbers is 10 in phase, and 5 in phase. (cckn-ia.org)

The multi-subunit shelterin complexes bind along mammalian telomeres, shielding the natural chromosome ends from engaging the DNA damage signaling and repair machinery [ 1 ]. (prolekarniky.cz)
The largest regions showing copy gain and loss are of 37.4 kb on chromosome 4, and 180.7 kb on chromosome 8. (biomedcentral.com)
Since hundreds of genes are present on chromosome arms, high-quality inferences of aneuploidy can be made from scRNA-seq datasets. (techscience.com)
Topoisomerases are nuclear enzymes that modify the topological state of DNA and participate in fundamental metabolic processes such as replication, transcription, repair, recombination, and chromosome segregation (1) . (aacrjournals.org)
The structure of the human and mouse gene contains eight coding exons and is located on chromosome 16 (28,41). (pancreapedia.org)

More than five decades ago, Jacob, Brenner, and Cuzin proposed the replicon hypothesis to explain the regulation of chromosomal DNA synthesis in E. coli. (wikipedia.org)
A fundamental feature of the replicon hypothesis is that it relies on positive regulation to control DNA replication onset, which can explain many experimental observations in bacterial and phage systems. (wikipedia.org)
In addition, a dramatic fruit size change due to a CNV with an insertion of 6-8 kb that affected gene regulation, was described during tomato breeding [ 27 ]. (biomedcentral.com)

For the genomic information, the researchers ran single nucleotide polymorphisms (SNP) microarray that interrogated 4,327,108 genetic variants selected from the International HapMap and 1,000 Genomes Projects and targeted genetic variation down to 1% minor allele frequency (MAF). (news-medical.net)
Seven of the 13 White look-alike doubles did not cluster genetically, indicating alternative purposes for shared genetic variation between look-alike pairs. (news-medical.net)
Little is known, however, about their contribution to genetic variation in rice. (biomedcentral.com)
taken together, The results suggest that shared genetic variation not only affects similar physical appearance, but may also influence common habits and behaviors. (wireservice.ca)
The named and biochemical disorders may or may not have been caused by their initially described genetic variation, but potentially by other gene variations. (medlink.com)

Depletion of TIN2 causes profound telomere deprotection phenotypes including destabilization of the shelterin complex, activation of telomeric DNA damage signaling, and increased apoptosis [ 9 , 11 - 14 ]. (prolekarniky.cz)
While it is considered primarily to be a digestive enzyme that functions to break down DNA in the alimentary tract (15, 32, 38) other functions have been suggested including degradation of DNA in apoptosis (45). (pancreapedia.org)

Regarding RNA sequence data, the principles of calculation methods are mainly divided into gene set enrichment analysis ( GSEA ) and deconvolution. (medicaltrend.org)
The significance of our research is the development of an SWGA DNA enrichment method that expands the range of clinical specimens that can be directly sequenced to include samples with low numbers of T. pallidum. (cdc.gov)

In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes. (neurotree.org)

T . equi has lost the putative genes for host cell transformation, or the genes were acquired by T . parva and T . annulata after divergence from T . equi . (biomedcentral.com)

The sources of these analyses are mainly DNA and RNA sequencing, especially the latter. (medicaltrend.org)
Previous analyses revealed that TE-mediated gene amplifications can have variable effects on fungal genomes, from inactivation of function to production of multiple active copies. (biomedcentral.com)
These analyses identified a housekeeping gene, histone H3, which was captured and amplified to hundreds of copies by a hAT DNA transposon, all of which were inactivated by RIP, except for the original. (biomedcentral.com)

Liu Y, Zhang YZ, Imoto S . Microbial Gene Ontology informed deep neural network for microbe functionality discovery in human diseases. (google.com)

The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)

In another wheat pathogen, Pyrenophora tritici-repentis , a histone H3 gene was amplified to tens of copies with little evidence of RIP, leading to many potentially active copies. (biomedcentral.com)

In peanut, the DNA polymorphism is very low despite enormous phenotypic variations. (biomedcentral.com)
The fact that the genotypic polymorphism is very limited despite enormous phenotypic differences among peanut genotypes signifies the requirement for a large number of markers. (biomedcentral.com)

The panel covered known genetic causes of channelopathies and cardiomyopathies and included a sequence analysis and copy number variation analysis of various genes [3]. (rnacentral.org)

The inactivation of MMR genes, usually hMSH2 or hMLH1, is associated with human colorectal cancers and is responsible for the characteristic microsatellite instability (MSI)+ phenotype of these tumors. (aacrjournals.org)

Most patients carry a missing or damaged p53 gene, a tumor suppressor whose activity is impaired in almost 50% of all cancers. (nature.com)
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)

Deformed epidermal autoregulatory factor-1 (DEAF-1) is a transcription factor that was originally shown to bind the autoregulatory enhancer of the Deformed ( Dfd ) Hox gene, which is activated in embryonic head segments of Drosophila (Gross, 1996). (sdbonline.org)
We show that, during embryonic devel-opment, the extramacrochaetae gene is expressed in complex patterns that comprise derivatives of the three embryonic layers. (biologists.com)
In view of gene dosage, a cell cycle is divided into an early stage and a late stage to measured in a mouse embryonic stem cell line. (cckn-ia.org)

We discuss the interplay between epigenetics and DNA sequence variation as well as the implications of epigenetics for cellular memory and plasticity. (nature.com)

One biologically relevant variable is readily inferred from scRNA-seq gene count tables regardless of individual gene representation within single cells: aneuploidy. (techscience.com)
Intuitive "eyeball" calls are uncommon in bulk RNA-seq or DNA-seq determination of aneuploidy and CNAs, so it is unsurprising that a simple stacking of read counts is insufficient for quality inference of aneuploidy in scRNA-seq data. (techscience.com)

However, it should be noted that when considering multiple tests, such as P-value analysis, the specific gene (MT-TW) did not show significant results [4]. (rnacentral.org)

Immunity genes are activated in the Drosophila fat body by Rel and GATA transcription factors. (sdbonline.org)

Gene signatures derived from the genes containing functionally germline variants significantly distinguished recurred and non-recurred patients in two ER+ breast cancer independent cohorts ( n = 200 and 295, P = 1.4 × 10 −3 ). (nature.com)
Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. (cancerindex.org)

This recognition comes as ultra-deep sequencing technologies coupled with error-reducing sequencing techniques have enabled the detection of mutant cells occupying less than one percent of an examined tissue sample ( Martincorena, 2019 ). (techscience.com)

B cells use unconventional strategies for the production of a seemingly unlimited number of antibodies from a very limited amount of DNA. (cipsm.de)

The moms plasma viral fill, Compact disc4+ T-cell count number, and HIV-1 genotype and subtype are summarized in Desk 1. (acusticavisual.net)

In general, cancer recurrence and metastasis are the result of the interactions of multiple mutated genes. (nature.com)
The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)

Extra-macrochaetae sequesters these basic-region-helix-loop-helix transcription factors as heterodimers inefficient for binding to DNA. (biologists.com)
The maintenance phase often involves a plethora of non-DNA sequence specific chromatin cofactors that set up and maintain chromatin states through cell division and for extended periods of time-sometimes in the absence of the initial transcription factors 3 . (nature.com)
Promoters and enhancers establish precise gene transcription patterns. (californiaehealth.org)
To see that GFP+ cells harbored LV transgenes with cloned NFR-DNAs with the capacity of activating transcription genomic DNA was ready Isoforskolin in the GFP+ transduced cells and utilized as template to recuperate the NFR-DNAs from integrated LV using PCR. (californiaehealth.org)
As usual, we use the mean, the noise, and the noise strength to characterize stochastic gene transcription. (cckn-ia.org)
It remains an essential and widely open question to quantify how the transition of cell cycle phases, the variation of DNA content and transcription kinetics in different phases, and the random partition of mRNAs in daughter cells affect the dynamics and noise of gene transcription. (cckn-ia.org)
Open in a separate window Fig FASLG 1 Coupling gene 21-Deacetoxy Deflazacort transcription with cell cycle.Actively dividing eukaryote cells go through phases in one cell. (cckn-ia.org)

Different types of markers have been developed and employed for diversity analysis, DNA finger-printing, trait mapping and genomics-assisted breeding (GAB). (biomedcentral.com)
Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. (techscience.com)
DNA saliva for multitomics analysis. (wireservice.ca)
Park H, Imoto S , Miyano S. Comprehensive information-based differential gene regulatory networks analysis (CIdrgn): Application to gastric cancer and chemotherapy-responsive gene network identification. (google.com)
Our analysis identified 50 genes that will be useful for definitive phylogenetic classification of T . equi and closely related organisms. (biomedcentral.com)

A CNV was described as early as 1936, with the duplication of the Bar gene in Drosophila melanogaster [ 6 ]. (biomedcentral.com)
The function of the Drosophila extramacrochaetae ( emc ) gene has been characterized almost exclusively in relation to the pat-terning of the fly's sensory organs (chaetae and other types of sensilla). (biologists.com)

Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes. (wikipedia.org)
This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses. (wikipedia.org)
Synthesis of daughter strands starts at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated. (wikipedia.org)
Although the specific replication origin organization structure and recognition varies from species to species, some common characteristics are shared. (wikipedia.org)
DNA replication is divided into different stages. (wikipedia.org)
During initiation, the replication machineries - termed replisomes - are assembled on DNA in a bidirectional fashion. (wikipedia.org)
These assembly loci constitute the start sites of DNA replication or replication origins. (wikipedia.org)
In the elongation phase, replisomes travel in opposite directions with the replication forks, unwinding the DNA helix and synthesizing complementary daughter DNA strands using both parental strands as templates. (wikipedia.org)
The necessity to regulate origin location likely arises from the need to coordinate DNA replication with other processes that act on the shared chromatin template to avoid DNA strand breaks and DNA damage. (wikipedia.org)
The model postulates that a diffusible, trans-acting factor, a so-called initiator, interacts with a cis-acting DNA element, the replicator, to promote replication onset at a nearby origin. (wikipedia.org)
Nonetheless, research subsequent to Jacob's, Brenner's and Cuzin's proposal of the replicon model has discovered many additional layers of replication control in bacteria and eukaryotes that comprise both positive and negative regulatory elements, highlighting both the complexity and the importance of restricting DNA replication temporally and spatially. (wikipedia.org)
The DNA mismatch repair (MMR) system is involved in the correction of base/base mismatches and insertion/deletion loops arising during replication. (aacrjournals.org)

The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)

The top category of Deaf1-regulated genes consists of glycolytic enzymes, which are suppressed by Deaf1 and Gsk3 but are upregulated by Deaf1 RNAi. (sdbonline.org)

Initially these disorders were named according to symptoms (later the cause was found to be mitochondrial dysfunction and more specifically maternally inherited mitochondrial DNA variations). (medlink.com)
Now, a variety of genes that cause mitochondrial disease have been discovered. (medlink.com)
T can be summarized as follows: This mutation firstly loses the appropriate base pair interaction, from Watson-Crick to T-G mismatching, in the anticodon-stem of MT-TW gene and induces defects in mitochondrial tRNATrp-associated translation machinery most likely due to inadequate anticodon recognition of mitochondrial tryptophan by its altered conformation [1]. (rnacentral.org)

to find regulatory genes of the achaete-scute complex (AS-C). The AS-C comprises four proneural genes that confer to cells the ability to become neuroblasts or sensory organ mother cells ( Campuzano and Modolell, 1992 , review). (biologists.com)

The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)

Our Core Cancer panels consist of essential gene sets that provide necessary information for genetic tumor biomarker profiling at the molecular level. (unilabs.ch)
Moreover, the rise of high-throughput sequencing has spawned an increasing number of studies on personalized cancer vaccines ( 13 ). (frontiersin.org)

In a recent study published in Cell Reports , researchers identified look-alike humans, who were genetically unrelated, using facial recognition (FR) algorithms for multi-omics studies. (news-medical.net)
As a control for a high similarity score, the team ran facial recognition algorithms on the photographs of monozygotic twins obtained from the University of Notre Dame twins database. (news-medical.net)

The defects suggest faulty specification of different cell types and result in impairment of processes as diverse as cell proliferation and commitment, cell adhesion and cell recognition. (biologists.com)
Although the technology to produce safe and effective iPSCs exists, study authors report they encountered an unexpected number of wobbly production processes for the cells. (neurosciencenews.com)

It also occurs at regions of specialized cell-cell contact and/or cell recognition, like the epidermal part of the muscle attachment sites and the differentiating CNS. (biologists.com)

These days, smartphones and closed-circuit television (CCTV) cameras use facial recognition software, igniting interest in normal-range face variations. (news-medical.net)
In no iteration of random gene sets did facial genes exceed the number of face genes represented in the 19,277 SNP selection. (news-medical.net)

These loci strongly correlated with annotated DNaseI-accessible loci in ESCs (AUROC = 0.86 Fig. 2a and Supplementary Number 1) and comprised approximately 4% of the total DNA within accessible chromatin of ESCs (Supplementary Notice). (californiaehealth.org)

Because MMR is assumed to modulate cytotoxicity to various chemotherapeutic agents that act upon DNA, our objectives have been to define its possible involvement in the cytotoxicity of topoisomerase inhibitors. (aacrjournals.org)

However, these changes are superimposed in individuals with reduced brain volume, especially in the hippocampus, and other developmental abnormalities, such as reduced dendritic arborizations, decreased number of spines, spine atrophy, and abnormalities of spine orientation in pyramidal neurons. (medscape.com)

Complicating this picture is that there are 2 sources of genetic material-maternal inherited mtDNA and nuclear DNA. (medlink.com)

In view of gene dosage, a cell cycle is divided into and stages. (cckn-ia.org)

The sample partitioning inherent for ddPCR considerably reduces the competition from any background DNA, allowing detection of minimal amounts of a target of interest. (biomedcentral.com)

Anatomy16

Organisms1

Diseases13

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology3

Phenomena and Processes56

Disciplines and Occupations4

Information Science6

Named Groups2

Health Care6

Sequences17

Methylation10

Mutations8

Genome19

Expression21

Proteins6

Somatic1

CNVs7

Alterations5

SNPs1

Assays1

Colorectal cancer cell lines1

Hybridization3

Immune7

MRNA2

Chromosome5

Regulation3

Genetic variation5

Apoptosis2

Enrichment2

Autism1

Putative1

Analyses3

Ontology1

Molecules1

Histone1

Phenotypic2

Sequence Analysis1

Inactivation1

Tumor suppressor2

Embryonic3

Cellular1

Aneuploidy2

Specific gene1

Immunity1

Variants2

20191

Antibodies1

Subtype1

Interactions2

Transcription7

Analysis5

Drosophila2

Replication12

Characteristics1

Enzymes1

Mitochondrial3

Regulatory1

Methods1

Cancer2

Algorithms2

Processes2

Epidermal1

Facial2

Chromatin1

Modulate1

Abnormalities1

Sources of genetic1

Dosage1

Detection1