Reagent nucleic acid hybridization. Medical search. Frequent questions

These samples would have to be separated in their own lanes inside the membranes and then the membrane would have to be rotated to a different angle where it would result in simultaneous hybridization with many different DNA probes. (wikipedia.org)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
Following appropriate sample preparation, the next step in solving the puzzle is to provide sufficient access for in situ hybridization probes to their targets. (bio-techne.com)
The proprietary formulations of these reagents have been optimized to provide enhanced access of in situ hybridization probes to nucleic acid targets across a wide variety of tissue sample types including FFPE, fresh‑frozen (FF), and fixed frozen tissue as well as tissue microarray (TMA) and cultured adherent and non-adherent cells. (bio-techne.com)
Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
Hybridization capture-based enrichment, which uses small bits of nucleic acid termed oligonucleotides as capture probes, is one method that allows for targeted sequencing. (qsstudy.com)
However, while hybridization capture-based enrichment is an effective method for targeted sequencing, commercially produced biotinylated capture probes are costly and can only be utilized for a limited number of reactions, making the per-sample cost for each capture process prohibitive. (qsstudy.com)
DSS offers a complete solution for FISH from reagents, probes, hybridization chambers to FL microscopes with accurate filters and software. (dssimage.com)
DIG-labeled hybridization probes are highly sensitive and suitable for low-(single)-copy gene detection of rare mRNA in Southern and northern blots. (thomassci.com)
DIG-labeled DNA probes have also been used for labeling of DNA during in situ hybridization. (thomassci.com)
My research focuses on the synthesis of modified nucleosides, oligonucleotides, metallated oligonucleotides, and their conjugation to develop hybridization probes. (utu.fi)
Modified nucleosides and nucleic acids, Oligonucleotide chemistry, developing covalently mercurated hybridization probes. (utu.fi)
The capture agents are then qualified by hybridization with synthetic probes to optimize hybridization and wash conditions for the genotyping panel. (arrayit.com)

Similar to solving a jigsaw puzzle, successfully performing the technique of in situ hybridization (ISH) benefits from a systematic, step-wise approach that concentrates on one stage of the process at a time, then moves onto the next. (bio-techne.com)
The ACD in situ hybridization kits (RNAscope, BaseScope™, miRNAscope™, and DNAscope™) can be used with formalin-fixed, paraffin-embedded (FFPE) samples, fresh-frozen (FF) or fixed frozen tissues, and both adherent and non-adherent cultured cells. (bio-techne.com)
In Situ Hybridization (ISH) is a powerful technique that allows you to identify and localize nucleic acid targets in formalin-fixed tissue sections and cells. (ahdiagnostics.dk)
Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
Abbott Molecular ThermoBrite® System offers an easy, safe, system for in-situ hybridization procedures. (dssimage.com)
What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)

RNAscope Protease III Reagent: A moderate protease treatment used with the chromogenic kits for cultured cells and with the fluorescent kits for fixed frozen tissue as well as cultured cells. (bio-techne.com)
RNAscope Protease IV Reagent: A strong protease treatment used with both the chromogenic and fluorescent kits for fresh-frozen tissue. (bio-techne.com)
More specifically, this invention relates to asymmetric benzoxanthene dyes useful as fluorescent labeling reagents. (justia.com)
The Label IT Nucleic Acid Labeling Reagents covalently attach CX-rhodamine, fluorescein, digoxin, biotin, Cy™3, Cy™5, dinitrophenol (DNP), and TM-rhodamine labels to nucleic acid bases within DNA or RNA without impacting hybridization performance, and therefore can be used in almost any molecular biology application (excitation and emission wavelengths for each fluorescent Label IT Reagent are listed in Table 1). (pdfmedarticles.com)
STREPTAMER(R) Best CELLer fluorescent MHC I Streptamer(R) reagents for detection and isolation of antigen-specific CD8+ T cells by flow cytometry select your. (biosave.com)
It is altered hybridization efficiency for each genotype call that leads to different fluorescent intensities in scanned microarray images (Figure 3). (arrayit.com)

FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)

Lastly, the Label IT® Nucleic Acid Labeling and Modifying kits include a few extra components compared to the Tracker™ kits: Denaturation Reagent D1, Neutralization Buffer N1 and G50 microspin purification columns. (mirusbio.com)
The G50 microspin purification columns are used to purify labeled nucleic acids from excess Label IT® reagents after the Label IT® reaction. (mirusbio.com)
Purification can also be performed with ethanol precipitation, which is described step-by-step in the protocols for all Label IT® kits and the page " Post-labeling Purification of Nucleic Acids with the Label IT® Reagents . (mirusbio.com)
We recommend using the Label IT® Nucleic Acid Labeling and Modifying kits if requiring a fluorophore or label that is unavailable in the Tracker™ formats and/or if the extra components (Denaturation Reagent D1, Neutralization Buffer N1 and G50 microspin purification columns) are required. (mirusbio.com)
Store all other supplied reagents, including the G50 microspin purification columns, at 4°C. Do not use the G50 microspin columns if they have been stored at -20°C or colder. (pdfmedarticles.com)
AF488 Nick Translation Labeling Kit contains all reagents (except template and materials for purification of the probe) required for nick translation labeling providing a highly efficient, easy-to-perform and rapid labeling technology. (jenabioscience.com)

Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
Grace Bio-Labs HybriWell ™ sealing system seals securely to a microscope slide surface in seconds to accommodate waterbath hybridizations, such as protein or nucleic acid hybridization assays. (sigmaaldrich.com)
The resultant DNA is suited for application in FISH, microarray gene expression profiling and other nucleic acid hybridization assays. (jenabioscience.com)
However, because reagents for these assays came from human volunteers, the reagents were available only in limited quantities and only at certain facilities. (cdc.gov)

The Selection Reagent differentiates hybridized from unhybridized probe, eliminating the generation of signal from unhybridized probe. (cdc.gov)
These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (thomassci.com)
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (thomassci.com)
The new guidelines further mention two specific antigen detection and nucleic acid probe-hybridization tests. (genomeweb.com)
Its key features include a comprehensive microbial databases are increasingly comprehensive, it is unlikely sequence database for probe design and protocols for sam- that more than a fraction of the existing microbial ple preparation, amplification, labeling, hybridization, and sequence space has been explored. (cdc.gov)
The initial and important step of FISH, CISH and ISH is to prepare your tissue for hybridization, before adding probe. (ahdiagnostics.dk)
One PCR labeling reaction (50 Î¼l) will typically yield enough probe for 20 ml hybridization solution. (thomassci.com)

It notably includes recommendations for use of nucleic acid amplification tests, or NAATs, for Trichomonas vaginalis and routine trichomonas screening for high-risk populations. (genomeweb.com)
Nucleic acid amplification tests (NAATs) are the recommended test method. (cdc.gov)
Thus, even in developed countries, only a small minority of clinical laboratories are capable of performing nucleic acid amplification tests (NAT's). (ref.ac.uk)

Label IT® CX-Rhodamine Labeling Kit Label IT® Fluorescein Labeling Kit Label IT® TM-Rhodamine Labeling Kit *Each Full Size Kit contains sufficient reagents to Label 100 µg of nucleic acid. (pdfmedarticles.com)
Each Trial Size Kit contains sufficient reagents to Label 25 µg of nucleic acid. (pdfmedarticles.com)

An amplification and labeling kit as well as all of the instruments, tools, reagents and buffers are provided to allow microarray-based genotyping at the customer location. (arrayit.com)

Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
In genomics, DNA-DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. (wikipedia.org)
The temperatures at which labelled DNA comes off reflects the amount of similarity between sequences (and the self-hybridization sample serves as a control). (wikipedia.org)
Critics argue that the technique is inaccurate for comparison of closely related species, as any attempt to measure differences between orthologous sequences between organisms is overwhelmed by the hybridization of paralogous sequences within an organism's genome. (wikipedia.org)
Molecular diagnostics refers to a set of laboratory tests that sequences the deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or cell proteins of an individual to analyze biomarkers of potential diseases. (researchandmarkets.com)
These oligonucleotides (also known as "oligos") are biotin-tagged and intended to hybridize to their targets based on nucleic acid sequence complementarity, allowing enabling simple capture and isolation of target sequences from biological samples. (qsstudy.com)
Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)

The assay can also be used as an analyte-specific reagent on urine or urethral swabs from men, if validated in a CLIA lab. (genomeweb.com)
Development of a dry-reagent-based nucleic acid-sensing platform by coupling thermostabilised LATE-PCR assay to an oligonucleotide-modified lateral flow biosensor. (peoriaobserver.com)
In this study, we report for the first time the development of a dry-reagent-based nucleic acid-sensing platform by combining a thermostabilised linear-after-the-exponential (LATE)-PCR assay with a one-step, Bio Med Frontiers hybridization-based nucleic acid lateral flow biosensor. (peoriaobserver.com)
Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). (caltech.edu)

Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)

This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly-deoxythymidine molecules that are covalently attached to the magnetic particles. (cdc.gov)

Not only must cell membranes be permeabilized for best results, but the crosslinking of nucleic acids to proteins that occurs during preparation of fixed samples can mask targets and must be relieved. (bio-techne.com)
Relative to proteins, complex nucleic acid-based systems can be designed more readily due to the countable nature of base-pairing interactions and readily available physical models. (caltech.edu)

We have adopted a staged strategy for molecular primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic pathogen surveillance and discovery. (cdc.gov)
Label IT® reagents are available in several formats, which provides researchers with a variety of options for their nucleic acid labeling experiments. (mirusbio.com)
Please reference the page " Adjust Labeling Density Using Label IT® Reagents " for more information on this topic. (mirusbio.com)
Instead, one might choose to use the Label IT® Nucleic Acid Labeling or Modifying kits if the fluorophore or label of interest is unavailable in the Tracker™ formats. (mirusbio.com)
In addition to Cy®3, Cy®5, fluorescein, CX-Rhodamine, TM-Rhodamine and biotin reagents, the Label IT® Nucleic Acid Labeling Kits are also available with MFP488, digoxin and DNP reagents. (mirusbio.com)
The Denaturation Reagent D1 and Neutralization Buffer N1 are primarily used for hybridization experiments (see Page 6 of the Label IT® Nucleic Acid Labeling Kit Full Protocol ). (mirusbio.com)
Mirus' Label IT Nucleic Acid Labeling Technology represents a new class of labeling reagents designed to efficiently and reproducibly attach marker molecules to nucleic acids (DNA or RNA) in a simple one-step reaction within minutes. (pdfmedarticles.com)
The simplicity of the Labeling reaction, and the fact that only a single reagent is required, suggests that this technology can be widely used in a variety of nucleic acid Labeling applications. (pdfmedarticles.com)
The Labeling efficiency of these reactions is dictated by the enzyme's ability to incorporate a 'Labeled-nucleotide' precursor into a growing nucleic acid chain. (pdfmedarticles.com)
If there is a need to increase or decrease the density of Labels in the final product, simply modify the ratio of Labeling reagent to nucleic acid during the Labeling reaction or adjust the incubation time of the labeling reaction. (pdfmedarticles.com)
Use only purified nucleic acid (A260/A280 between 1.8 and 2.2) in the Labeling reactions. (pdfmedarticles.com)
Label IT Nucleic Acid Labeling Reagents for efficient, direct, non-enzymatic labeling of DNA and RNA. (fishersci.com)
This product is designed as a safer chemical.Â The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. (thomassci.com)
There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. (thomassci.com)

Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)
In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods. (justia.com)
The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

Current membrane-based lateral flow tests are rapid because no complex reagent additions, washing or incubation steps are required. (ref.ac.uk)
The simplicity of the user experience after the test begins is maintained by using a disposable 3-channel lateral flow device to automatically deliver reagents to the test region in three successive stages without user interaction. (caltech.edu)

Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. (thomassci.com)
Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon. (aidstar-one.com)

Among the products & services included in the report, in 2023, the kits & reagents segment is expected to account for the largest share of the Europe molecular diagnostics market. (meticulousresearch.com)
These features of nucleic acids enable us to design novel interaction pathways and functions by providing well-behaved molecular mechanisms. (caltech.edu)
This thesis will first demonstrate the use of these nucleic acid molecular mechanisms in closed-loop genetic circuits and infectious disease testing using cgRNAs and HCR, respectively, then provide updated tools for the nucleic acid design community to exploit the programmable nature of nucleic acids. (caltech.edu)
NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)
Based on product, the reagent segment of the infectious disease molecular diagnostics market is leading because it aids in rapid and sensitive amplification of RNA and DNA, allowing for faster results by speeding up processing. (clothingsuite.com)

Two generic platform technologies that greatly improve the performance of rapid tests have since been developed: the S ignal A mplification S ystem ( SAS ) for protein-based targets (Lin et al, JCM 2008), and S imple AM plification B ased A ssay ( SAMBA ) for nucleic acid-based targets (Lee et al, JID 2010). (ref.ac.uk)

During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
Nucleic acid nanotechnologies have provided a platform where biologically relevant molecules can be engineered to perform programmable functions. (caltech.edu)
Using cheap reagents and an easy experimental workflow, TEQUILA-seq allows us to deeply sequence the full-length RNA molecules for any gene set across many biological samples," said co-senior author Yi Xing, Ph.D., head of CHOP's Center for Computational and Genomic Medicine. (qsstudy.com)

once labeled, nucleic acids can be further coupled to NH 2 reactive substrates or chemicals. (mirusbio.com)

The increasing approval rate of novel product lines, including devices and reagents for Bloodstream Infections (BSI) diagnostics, will offer more profitable and attractive possibilities for the blood culture testing market. (globalmarketestimates.com)
Our platform is universal for nucleic acid-based genetic screening, testing, diagnostics, genotyping and single nucleotide polymorphism (SNP) analysis. (arrayit.com)

However, the lack of access to RT-qPCR instruments, reagents and trained instrument operators can limit the utility of this diagnostic tool, especially in resource-constrained developing countries. (nature.com)

However, abundance of host RNA (especially rRNA) and other environmental nucleic acids can reduce the abundance of pathogen specific reads of interest, reduce depth of coverage and increase surveillance costs. (bvsalud.org)

NOTE: All the Label IT Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra. (fishersci.com)
Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. (bvsalud.org)

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. (thomassci.com)
NOTE: the Label IT® reagent (µl):nucleic acid (µg) ratio (v:w) listed in the table is the recommended starting value prescribed in each kit's respective protocol. (mirusbio.com)

Dilution of concentrated acids: formula etc. (biologicalstaincommission.org)

It was used as negative control in DNA-DNA hybridization experiments using genomic DNA preparations of Vibrio parahaemolyticus and Vibrio alginolyticus. (thomassci.com)
HyperChrome is the perfect Instrument for denaturation & hybridization procedures during FISH experiments. (dssimage.com)

A specific fragment pattern is detected after hybridization of the PCR product to 10μg human genomic DNA followed by chemiluminescent detection. (thomassci.com)

RNAscope Hydrogen Peroxide Reagent: For blocking endogenous peroxidase activity with use of the chromogenic kits. (bio-techne.com)
RNAscope Protease Plus Reagent: A mild protease treatment used with the chromogenic kits for FFPE and fixed frozen tissue. (bio-techne.com)

We provide bovine and human serum albumins and transport protein reagents for diverse applications, including cell culture, protein quantitation, and antibody-related studies. (sigmaaldrich.id)

and reagents for use in turbidimetry and flow cytometry, as well as develops liquid-based pharmacodiagnostics. (danelfin.com)

In 1969, one such method was performed by Mary Lou Pardue and Joseph G. Gall at the Yale University through radioactivity where it involved the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation, which is identified as autoradiography. (wikipedia.org)

1. Prior to each use, warm the vial containing the Label IT Reagent to room temperature and quick spin to collect the pellet. (pdfmedarticles.com)

The modern approach is to carry out DNA-DNA hybridization in silico utilizes completely or partially sequenced genomes. (wikipedia.org)
DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. (bvsalud.org)

RNAscope kits already include pretreatment reagents appropriate for their most typical use, but certain combinations of kits and tissues will require additional reagents. (bio-techne.com)

In the first stage we acid extracted from clinical materials and cultured isolates. (cdc.gov)

Reagent is stable for 6 months after reconstitution. (pdfmedarticles.com)
Unreconstituted Label IT Reagent and all other reagents are stable for up to 1 year from the date of purchase. (pdfmedarticles.com)
The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation. (vectorlabs.com)

It is a bacterial infectious disease referred to the National Tuberculosis phenol-chloroform reagent was added that is considered the second most Institute in Sana'a city with suspected and the mixture was vortexed for about important cause of death due to an pulmonary TB based on their presen- 10 s and heated at 80 °C for 20 min. (who.int)

Two examples of these mechanisms are the conditional guide RNA (cgRNA) and the hybridization chain reaction (HCR). (caltech.edu)
We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)

By eliminating the need for radioactive labels, safety is enhanced and the environmental impact of reagent disposal is greatly reduced, resulting in decreased costs for analysis. (justia.com)
It is a hybridization-based analysis of SNPs, mutations, deletions, insertion-deletion mutations (indels), and other minor sequence variations from any organism of interest. (arrayit.com)

In addition, these types of reactions generate Labeled products that are not representative of the starting nucleic acid but rather consist of a series of Labeled samples over a variable size range. (pdfmedarticles.com)
Where my research was focused on the biomimetic total Synthesis of complex natural products mainly bioactive lactones from carbohydrate scaffolds and Artemisinic acid glycoconjugates synthesis. (utu.fi)

In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. (justia.com)
I later moved to the University at Albany, State University of New York (SUNY), USA for my postdoctoral research, where I worked on α-functionalization of Cysteine type amino acids with various alkyl halides using photocatalysis and dimeric compounds synthesis through dearomatization reactions. (utu.fi)

Anatomy1

Organisms4

Chemicals and Drugs25

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes15

Information Science4

Health Care3

Probes14

Situ6

Fluorescent6

Polymerase Chain Re1

Purification6

Assays4

Probe7

Amplification tests3

Contains sufficient reagents2

Microarray1

Sequences7

Assay4

Isothermal amplification1

Covalently1

Proteins2

Labeling14

Sequencing3

Lateral flow2

Deoxyribonucleic2

Molecular5

Targets1

Molecules3

Substrates1

Diagnostics2

Instruments1

Pathogen1

Technology2

Ratio2

Dilution1

Experiments2

Genomic1

Chromogenic2

Protein1

Flow cytometry1

Radioactivity1

Temperature1

Utilizes2

Kits1

Isolates1

Stable3

Tuberculosis1

Reaction2

Analysis2

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