• Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • The biological activity of HTS02 is assessed by a tubulin polymerization assay. (cytoskeleton.com)
  • Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
  • To a synthetic chemist, it provides an opportunity to synthesize a library of compounds based on a core scaffold and to screen the derivatives for various biological activities. (ijpsonline.com)
  • While the epitope of most B7-H3 antibodies has not been described, one of the clinically tested antitumor antibodies (8H9) was found to bind to the IgV domain 19 . (nature.com)
  • It has been shown that within the variable regions of even fully human antibodies, somatic mutations and other alterations in sequence information can stimulate a powerful reaction on the part of the host. (genengnews.com)
  • Antimicrobial activity was screened in vitro against Pseudomonas sp. (ijpsonline.com)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • Triticum aestivum L. root growth inhibition test and Allium cepa L. assay were used to assess the cytotoxic potential of phosphonates. (japsonline.com)
  • The activity of this enzyme is commonly assayed using a leucine arylamide chromogenic substrate such as leucyl beta-naphthylamide. (lookformedical.com)
  • Dr J J Keating (University College Cork) is one of a small group of chemists who synthesise drugs of abuse such as, for example, MDMA (more commonly known as ecstasy) and want the world to know about it! (soci.org)
  • Cyclophosphamide (CTX) is a commonly used alkylating anti-tumor drug. (peroxidasessignaling.com)
  • Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
  • 2013. Brine shrimp lethality assay 'an effective prescreen': Microwave-assisted synthesis, BSL toxicity and 3DQSAR studies-based designing, docking and antitumor evaluation of potent chalcones. (cytoskeleton.com)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
  • The present work focused on (1) synthesis of β-amino- and β-iminophosphonates via a nucleophilic addition reaction of benzylamine and 2-aminopirydine to 1,2-alkadienephosphonates, i.e., dialkyl esters and amidoesters, as well as dimethyl- and diphenylphosphine oxides, and (2) investigation of cytotoxicity and anticancer potential of synthesized compounds. (japsonline.com)
  • It persisted at this high level till antimalarial drugs were administered. (who.int)
  • Regardless of the therapeutic approach, the heterogeneity of pancreatic cancer cells and the complexity of its tumor microenvironment contribute to the emergence of drug resistance. (frontiersin.org)
  • When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)
  • Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
  • In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
  • N- trans -Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. (hindawi.com)
  • H3.3 mutant gliomas are also particularly sensitive to NOTCH pathway gene knockdown and drug inhibition, reducing their viability in culture. (nature.com)
  • Cell proliferation assays demonstrated that treatment with the more than 10,000 molecular weight fraction significantly inhibited viability in DLD‑1 cells. (spandidos-publications.com)
  • All the starting compounds have never been studied in the discussed reaction. (japsonline.com)
  • It is reported that the compounds bearing O-H bonds behave as antioxidants but along with them, amines having N-H bond functions as the antioxidant, which have attracted much research attention because cyclic amines have always been the central structure in many currently used drugs [ 11 ]. (ijpsonline.com)
  • The present study aimed to investigate components other than phenolic compounds that may have antitumor effects against colorectal cancer (CRC). (spandidos-publications.com)
  • Therefore, AGEs in MSpf may be potential compounds for the development of antitumor drugs for the treatment of CRC with fewer adverse effects compared with existing antitumor drugs. (spandidos-publications.com)
  • It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
  • This one-day conference held on 1 July 2008, and sponsored by SCI, the RSC, and ICI brought together scientists from the Republic of Ireland and from Northern Ireland who have varied interests in drug discovery and development and a common interest in synthesis. (soci.org)
  • Professor Maguire underlined the central importance of organic synthesis to the field of chemical biology and the interfaces with drug development and process development. (soci.org)
  • Of course, clandestine synthesis of ecstasy and other drugs of abuse is widespread. (soci.org)
  • Treatment options are limited at best and drug resistance is common. (frontiersin.org)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • Chemotherapeutic drugs result in not only strong resistance but also more side effects [ 4 ]. (hindawi.com)
  • Diagnostic testing continues to improve, but multi-drug resistance to treatment is an increasing challenge. (cdc.gov)
  • Tubulin polymerization must also be responsive to polymerization enhancers (paclitaxel) and inhibitors (nocodazole) at 5 µM drug concentration. (cytoskeleton.com)
  • Some Mek inhibitors showed promising antitumor activity, although schedules and doses associated with low systemic toxicity need to be defined. (biomedcentral.com)
  • Finally, several clinical trials are currently ongoing using MEK inhibitors in combination with chemotherapeutic drugs (including dacarbazine or paclitaxel). (biomedcentral.com)
  • Dr. Baker and his colleagues were successful in synthesizing a peptide containing two mutations, which provoked a greatly reduced T-cell reaction in healthy individuals. (genengnews.com)
  • These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
  • Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)
  • The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
  • The major concern of chemotherapy is the side effects of existing drugs ( Baldo and Pham, 2013 ). (japsonline.com)
  • Reciprocal editing of cells generally produced reciprocal effects on tumorgenicity in xenograft assays. (nature.com)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • This symposium presents a remarkable opportunity to convene with other researchers, clinicians, and industry leaders to discuss the extraordinary progress and immense potential of new drug therapies and technologies in paving the way for a brighter future in cancer treatment. (nyas.org)
  • Besides, recent efforts have focused on developing targeted therapies for pancreatic cancer, including drugs that target specific molecular alterations like the KRAS oncogene, which is frequently mutated in pancreatic cancer ( 9 - 11 ). (frontiersin.org)
  • 2019). Thus, screening effective immunopotentiator is benefit for aquaculture, which may reduce the use of antibiotics. (peroxidasessignaling.com)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • In 2D culture, all cells can proliferate at a relatively constant rate in a planar space, and can be provided uniform access to nutrients, oxygen or drugs. (medsci.org)
  • Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. (hindawi.com)
  • Herein, genome-wide transcriptome profiling (RNA-Seq) was used to screen the differentially expressed genes (DEGs) in FO-treated Huh7 cells, with the aim of uncovering the pharmacologic pattern. (hindawi.com)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • However, the lack of ideal immunosuppressive models has limited the screening and evaluation of immunopotentiator in fish. (peroxidasessignaling.com)
  • When ingested in culinary quantities, fenugreek is usually devoid of adverse reactions. (drugs.com)
  • Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
  • SPPS allows a rapid assembly of a peptide chain through successive reactions of amino acid derivatives on an insoluble porous support. (rgcb.res.in)
  • The first example of the reaction of amines with substituted allenephosphonic acid derivatives was published in 1966 ( Pudovik and Khusainova, 1966 ) when it was shown that diethylamine and piperidine add to dialkyl 3-methyl-1,2-butadienephosphonates on the 1,2-double bond to afford 1,2-aducts. (japsonline.com)
  • thus, these drugs have no effect on wild type RAS at the tested doses (4) . (cytoskeleton.com)
  • Premedicate Kyprolis infusions with dexamethasone prior to all Cycle 1 doses and if infusion reaction symptoms develop or reappear. (aetna.com)
  • The clandestine chemist has to switch their method of production according to the availability of different starting materials, thus a number of synthetic routes are available to a given drug molecule. (soci.org)
  • Histamine (4-(2-Aminoethyl)-1 H -imidazole, see Scheme 2 ) is a molecule that performs various functions in the body, the most important being the gastric acid secretion and the triggering of the symptoms of an allergic reaction such as vasodilatation, bronchoconstriction, bronchial muscle contraction, pain, and itching. (hindawi.com)
  • Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
  • The drug underwent significant rounds of modifications to produce a drug suitable for in vivo applications, these efforts resulted in ARS-1620 which has been used by the scientific community to further define the utility of this class of drugs (5) . (cytoskeleton.com)
  • Keating's programme aims to elucidate full impurity profiles for drugs in relation to the full synthetic pathway employed. (soci.org)
  • Switches occur when patients receive medicines formally designated as biosimilars, but may also occur after manufacturing process changes have occurred, if the process changes lead to structural modifications or changes in the impurity profile of the biologic drug [ 5 ]. (springer.com)
  • Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
  • Recently, a drug that targets mutant RAS G12C specifically has shown promising clinical results and is now the first FDA approved RAS-targeting drug for the treatment of NSCLC, read on for a summary of this drugs journey from discovery to approval, and roadblocks that still lie ahead. (cytoskeleton.com)
  • We obtained protein components from maple syrup by ammonium sulfate precipitation, and treatment with the protein fraction of maple syrup (MSpf) was found to exhibit a potential antitumor effect. (spandidos-publications.com)
  • Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • The second section will address novel agents associated with liver injury in 2014 (referred to as "signals"), especially in terms of clinical, research and drug development implications. (wjgnet.com)
  • Protein quantitation was performed using the Pr ecision Red Protein Assay Reagent (Cat. (cytoskeleton.com)
  • An ASSAY UNIT is defined as the amount of HTS02 protein needed to achieve a tubulin polymerization signal of OD340 of 0.1 - 0.15 in 30 minutes at 37°C in G-PEM buffer. (cytoskeleton.com)
  • The protein concentration for HTS02 in this assay is approximately 4 mg/ml. (cytoskeleton.com)
  • Although the RAS family of proteins was discovered nearly four decades ago there has not been a viable drug therapy developed to effectively blocks mutant RAS dysfunction. (cytoskeleton.com)
  • Drug discontinuation and therapy reconciliation remain the mainstay in patent's management to minimize occurrence of acute liver failure. (wjgnet.com)
  • A physico-chemical screening test for chemical carcinogens: The ke test. (cdc.gov)
  • Alternative drugs have been developed to target Farnesyl transferase and downstream MEK targets, but these have failed for various reasons (reviewed in (1, 2)) . (cytoskeleton.com)
  • Having in mind all these facts and the procedures described previously, we have investigated the same reaction applying an atom economy, environment-friendly, and easy for industrial application synthetic protocol, as well as different starting 1,2-alkadienephosphonates. (japsonline.com)
  • Drug-induced liver injury (DILI) and herb-induced liver injury is a hot topic for clinicians, academia, drug companies and regulators, as shown by the steadily increasing number of publications in the past 15 years. (wjgnet.com)
  • Zhu K, Aykas DP and Rodriguez-Saona LE: Pattern recognition approach for the screening of potential adulteration of traditional and bourbon barrel-aged maple syrups by spectral fingerprinting and classical methods. (spandidos-publications.com)
  • It has been formulated to be compatible with 96 or 384-well format polymerization assays such as in Biochem™ kits BK004 or BK011P or CytoDYNAMIX Screen™ BK004. (cytoskeleton.com)
  • As urinary screens, they are noninvasve and apble on a large scale with current labo techniques For short-term esue, glucaric acid, thloetbers, and mug tests are usefl. (cdc.gov)
  • Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
  • Since the clandestine chemist is unlikely to engage in rigorous purification of their products, most drugs of abuse contain significant impurities. (soci.org)
  • Incorporation of the non-acidic thiol, mercaptoethanol into the reaction mixture, caused significant activation of the invertase. (who.int)
  • This may be of concern to the user, but Keating is most interested in the opportunities this gives for forensic linkage of recovered drugs to their production site. (soci.org)
  • Hence, relatively straightforward analytical detection of this pyridine can instantly link, or not, a particular batch of drug to a particular clandestine production facility. (soci.org)
  • Finally, the gene expression of Atf4, Ire1α, Xbp1s, Xbp1t, Grp78 and Chop was measured from these cDNA samples by qPCR (quantitative polymerase chain reaction). (helsinki.fi)
  • Indoles of commercial importance include drugs such as indomethacin (antiinflammatory, an antipyretic and an analgesic) and indoxole (an antiinflammatory and antipyretic drug). (ijpsonline.com)