AffinityElectrophoresisCoupled to agarose beads followedBeadsImmunoaffinity chromatographyHPLCCharged resinPolyacrylamideAntibodyFPLCPurityBufferProteinCellulosePolymeraseMixtureBiotechnologyCompoundsBulkSequenceConcentrationLayerCommonlyDetectionInhibitCross-linkedConsistsSynthesisEnzymeTypeColumnAbilityStableSolidConditionsSizeThinAdditionRemoveHuman
Affinity11
- Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. (wikipedia.org)
- Like general chromatography, but using dyes to apply on a support matrix of a column as the stationary phase that will allow a range of proteins with similar active sites to bind to, refers to as pseudo-affinity. (wikipedia.org)
- This include topics on Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Gas Chromatography Mass Spectrometry (GC-MS), Liquid Chromatography Mass Spectrometry (LS-MS), and Affinity Chromatography. (elac.edu)
- A serine protease was purified from poultry organic dust by benzamidine-agarose affinity chromatography. (cdc.gov)
- Owing to the presence of a His tag , EK1 can be easily removed after the cleavage reaction by affinity chromatography with Ni-IDA Agarose Beads. (abmgood.com)
- In addition, DDDDK is a part of the octapeptide FLAG tag (DYKDDDDK), which can be utilized as a fusion tag for recognition by antibody, and for detection of fusion protein expression with Western blot analysis, as well as for purification of the fusion protein by Anti-FLAG affinity chromatography. (abmgood.com)
- It is not generally necessary to deactivate the EK enzyme, however to prevent further degradation of the target protein after the reaction is finished, removal of enterokinase via affinity chromatography is recommended. (abmgood.com)
- Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates. (goldbio.com)
- GSTCSTIL fragments had Levosimendan been portrayed in BL21-Rosetta and natively purified by single-step affinity chromatography using glutathione-agarose based on the manufacturer's process. (stemcellresearchformichigan.com)
- Specific substrates generally bind with sufficient affinity that they remain bound during multiple ubiquitin transfer reactions, resulting in processive substrate modification [ 9 , 10 ]. (biomedcentral.com)
- Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
Electrophoresis3
- Techniques include protein isolation, purification and characterization, protein assays, immunoassay: Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, Sodium Dodecyl Sulfate Polyacrylamide (SDS-PAGE), agarose gel electrophoresis, and Polymerase Chain Reaction (PCR) technology. (elac.edu)
- This was caused by additional excretion of high density lipoproteins or both high and low density lipoproteins in the urine which could be identified in these fractions by agarose electrophoresis and immunodiffusion. (uni-muenchen.de)
- The physicochemical characteristics of C 60 -Dex-NH 2 was examined with elemental analyses, gel permeation chromatography, solid-state nuclear magnetic resonance ( 13 C, HPDEC), agarose gel electrophoresis, and dynamic light scattering. (researcher-app.com)
Coupled to agarose beads followed2
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. (caslab.com)
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. (rockland.com)
Beads5
- In fast-protein liquid chromatography (FPLC) using Blue MX-R immobilized on poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads, it was seen to separate lysozyme and bovine serum albumin (BSA), purified lysozyme from chicken albumin. (wikipedia.org)
- Thiolsulphinate groups have been introduced on to pearl-polymerized agarose, cross-linked dextran, microcrystalline cellulose, polyacrylamide-dextran co-polymers, co-polymerized (ethylene glycol)-methacrylate, acrylic beads and porous glass. (fq.edu.uy)
- IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. (goldbio.com)
- Flag-STIL destined to agarose beads was cleaned 3 x in NP40 buffer as soon as in kinase buffer (50?mM Tris pH?7.5, 10?mM MgCl2, 10?M MnCl2, 1?mM DTT) accompanied by an incubation with 2.5C5?g ZzCPlk4-His in the current presence of 5?Ci [-32P]-ATP (PerkinElmer) in kinase assay buffer supplemented with 33?M ATP for 15C20?min in 30C. (stemcellresearchformichigan.com)
- The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. (jacksonimmuno.com)
Immunoaffinity chromatography1
- Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. (jacksonimmuno.com)
HPLC1
- It can carry out in a conventional way by using as a packed column, or in high-performance liquid chromatography (HPLC) column. (wikipedia.org)
Charged resin1
- A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (lookformedical.com)
Polyacrylamide1
- The commonly used supporting matrix would be cross-linked agarose (sepharose), sephadex, polyacrylamide, and silica. (wikipedia.org)
Antibody2
- Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. (rockland.com)
- The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species. (jacksonimmuno.com)
FPLC1
- Nickel agarose resin is suitable for use with FPLC, but with pressures no more than 20 kPa . (goldbio.com)
Purity1
- However, a good yield of the DNA library was achieved by using trypticase in the culture media and high purity agarose as the top agar during the amplification. (gla.ac.uk)
Buffer4
- One unit is defined as the amount of TEV Protease that is required to cleave >90% of 3 µg of control substrate in a 30 µl reaction for 1 hour at 30°C in 1X TEV Protease Reaction Buffer supplemented with 1 mM DTT. (abmgood.com)
- Enzyme supplied with 20X Reaction Buffer. (abmgood.com)
- GoldBio Nickel Agarose works very well with the His-Tag Buffer Set. (goldbio.com)
- Reactions had been stopped with the addition of test buffer, elution for 10?min in RT and heating system at 95C. (stemcellresearchformichigan.com)
Protein2
- On agarose as supporting matrix, it was seen to purify cholesteryl ester transfer protein. (wikipedia.org)
- The kit is suitable for 50 reactions using 10µl Immobilized Protein A/G Agarose. (gbiosciences.com)
Cellulose1
- The two activities copurify during chromatography using phosphocellulose, heparin-agarose, or double-strand DNA-cellulose, and during velocity sedimentation in a glycerol gradient containing 0.5 M KCl. (neb.com)
Polymerase3
- Labelling of the library by nick-translation and random priming did not achieve decoration of the whole chromosome 21 but direct labelling of Biotin-ll-dUTP by polymerase chain reaction (PCR) amplification was found to be efficient and overcame the problem of non-homogenous painting of the target chromosome. (gla.ac.uk)
- Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
- Under polymerization conditions, mismatch excision by the exonuclease occurs prior to polymerization by polymerase gamma, and this excision can be inhibited by adding to the reaction a high concentration of dNTP substrates and/or nucleoside 5'-monophosphates. (neb.com)
Mixture2
- Visionlearning Vol. Download our nonpositive mixture for stone and need chromatography! (senecadevelopmentne.com)
- Chromatography is common way in which components of a mixture are separated. (monmouthcollege.edu)
Biotechnology1
- Synthesis of Lactulose in Continuous Stirred Tank Reactor With β-Galactosidase of Apergillus oryzae Immobilized in Monofunctional Glyoxyl Agarose Support" Biotechnology and Bioingenieering. (abtbeads.com)
Compounds4
- A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
- A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
- Also, thin layer chromatography (TLC) plates can be visualized if the compounds or a coloring reagent is fluorescent. (newworldencyclopedia.org)
- Monmouth also uses some instruments that have particular roles, such as measuring the heat or energy of a reaction, or the surface tension of a layer of compounds. (monmouthcollege.edu)
Bulk1
- Nickel Agarose Resin is available in bulk or in prepacked columns. (goldbio.com)
Sequence1
- Unlike other site-specific proteases that cut within the recognition sequences leaving extra amino acids in the cleaved peptide products, the EK1 cleaves after the C-terminal end of the lysine residue, and the fragment produced from the cleavage reaction does not inherit any residues from the DDDDK recognition sequence. (abmgood.com)
Concentration1
- With agarose, degrees of thiolsulphinate substitution ranging from 100 to 500 μmol/g of dried derivative can be easily prepared from the same thiol-agarose batch by varying the concentration of the reagent used in the second oxidation step. (fq.edu.uy)
Layer1
- Chromatography thin layer. (lookformedical.com)
Commonly2
- Commonly used reactive dyes for chromatography can be separated according to their color index name or functional group. (wikipedia.org)
- It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
Detection1
- Biological molecules can be tagged with a fluorescent chemical group (fluorophore) by a simple chemical reaction , and the fluorescence of the tag enables sensitive and quantitative detection of the molecule. (newworldencyclopedia.org)
Inhibit1
- Lower fidelity is observed using reaction conditions that inhibit the exonuclease activity, strongly suggesting that the exonuclease proofreads errors during polymerization. (neb.com)
Cross-linked1
- IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. (goldbio.com)
Consists1
- The procedure consists of a thiolation and two oxidation reactions. (fq.edu.uy)
Synthesis2
- Glycosynthase Reaction Meets the Flow: Continuous Synthesis of Lacto-N-Triose II by Engineered β-Hexosaminidase Immobilized on Solid Support. (abtbeads.com)
- Cationic macromolecules are attractive for use as small interfering RNA (siRNA) carriers due to their performance in non-immunological reactions, customization during synthesis, and low costs compared to viral carriers. (researcher-app.com)
Enzyme1
- similar to that of CYP2C9, thereby obviating kinetic approaches for determining the contribution of the former enzyme to this microsomal drug-metabolizing reaction. (aspetjournals.org)
Type2
- C29H20ClN7O11S3 + C24H38O19 → C53H57N7O30S3 + HCl Cibacron Blue FG3-A + Sepharose → Blue Sepharose + HCl The dyes used in this type of chromatography are inexpensive and generally available as they are from textile industries called reactive dye. (wikipedia.org)
- Using heparin chromatography, we separated mature HPV type 16 PsVs into three fractions (I, II, and III) according to their heparin-binding affinities. (biomedcentral.com)
Column1
- These were overcome by cleaning with Sephadex G-50 column chromatography and freeze drying of eluate. (gla.ac.uk)
Ability1
- The thiolsulphinate-agarose had the ability to bind up to 500 μmol of low-Mr thiols/g of dried gel. (fq.edu.uy)
Stable1
- Its activity is stable over a broad temperature range, and it completes the reaction from 4°C to 60°C. (abmgood.com)
Solid1
- Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
Conditions1
- The experimental conditions for the introduction of thiolsulphinate groups on to agarose have been optimized, as this is one of the most widely used supports in chromatography. (fq.edu.uy)
Size1
- 6% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. (abtbeads.com)
Thin1
- Chromatography on thin layers of adsorbents rather than in columns. (lookformedical.com)
Addition1
- In addition, as tris has a corrosive effect on metals such as copper and aluminium, it needs to be stored separately to avoid unnecessary chemical reactions. (hbdsbio.com)
Remove1
- Yes, this method can be used to remove enterokinase following the cleavage reaction. (abmgood.com)
Human3
- Because it is produced using human cell line, it has the appropriate glycosylation to catalyze highly accurate cleavage reaction with minimum non-specific activity. (abmgood.com)
- No reaction was observed against Human IgM or Human IgA. (rockland.com)
- Immunodiffusion studies showed in about 40% precipitin lines against anti-human albumin, but no reactions against anti-human apoHDL and anti-human apoLDL. (uni-muenchen.de)