• genome
  • In this paper, RAPD primers were used to test whether accelerated germination induced by qi energy was associated with changes in the genome. (healingtaousa.com)
  • Citrus has a small genome (1C = 0.62 pg) (Guerra, 1984), is diploid with a small number of chromosomes (n = 9) (Soost and Cameron, 1975) and is highly polymorphic. (scielo.br)
  • RAPD data of these two populations, one from the mountains of central Norway and the other from the coastal areas, were analyzed to determine whether these differences are due to divergence in the plant genome or they are merely products of environmentally induced changes. (uio.no)
  • Abstract
  • Burrows, P.R., 1988, The differentiation of Globodera pallida from G. rostochiensis using species-specific DNA probes, Nematologica (Abstract. (springer.com)
  • primer
  • For a RAPD band to be produced, the primer needs to match a binding site that is within approximately 2-3 kilobase pairs of another oppositely oriented binding site so that the single oligonucleotide can prime replication in both the forward and reverse directions. (bioline.org.br)
  • A total of 130 loci were generated outof which 98 were polymorphic by 15 primers with 05-14 loci/primer having fragment's size range from 400to 2500bp maximum. (thefreelibrary.com)
  • This is achieved one or two ways: 1) a DNA target is amplified at the same time via primer sets, or 2) different DNA targets are amplified independently via particular primers. (wikipedia.org)
  • genomic
  • This report summarizes the results of our efforts to generate useful induced mutants of the Philippine banana cultivars, 'Latundan' and 'Lakatan' through irradiation, and to evaluate the usefulness of DNA marker techniques, such as RAPD, microsatellites or SSR, and AFLP, to characterize the genomic alterations in induced mutants of the two Philippine banana cultivars. (fao.org)
  • Atienzar F, Evenden A, Jha A, Savva D, Depledge M. Optimized RAPD analysis generates high-quality genomic DNA profiles at high annealing temperature. (springer.com)
  • 1996
  • Ioannou, Y.A. and Chen, F.W. (1996) Quantitation of DNA Fragmentation in Apoptosis. (scirp.org)
  • Savva, D. (1996) Use of DNA Fingerprinting to Detect Genotoxic Effect. (scirp.org)
  • clones
  • It can also detect variation in DNA profiles of induced mutant clones which are otherwise morphologically indistinguishable, and detect variation between the induced mutant parent clones and their derived suckers. (fao.org)
  • restriction
  • Burrows, P.R., and Boffey, S.A., 1986, A technique for the extraction and restriction endonuclease digestion of total DNA from Globodera rostochiensis and G. pallida second stage juveniles, Revue Nematol . (springer.com)
  • The DNA is digested by a particular restriction enzyme, resulting in DNA pieces of varying molecular masses. (wikipedia.org)
  • On the other hand, the size of the DNA pieces are based on the sites where the restriction enzyme cuts. (wikipedia.org)
  • variation
  • Induced mutation (either by chemicals or irradiation), coupled with in vitro propagation technique such as shoot-tip culture, has been established as a tool to generate variation in a number of vegetatively propagated crops [2- (fao.org)
  • Freeman, J.L. and Rayburn, A.L. (2004) Metamorphosis in Xenopus laevis Is Not Associated with Large-Scale Nuclear DNA Content Variation. (scirp.org)
  • Small
  • Spin column chromatography was used to extract DNA from small broomrape plants collected from the original infested field in Clackamas County, Oregon (DNeasy Plant Mini Kit, Qiagen, Inc., Valencia, CA). The ITS region of the nrDNA was amplified with the universal primers ITS5 and ITS4 as described (13). (plantmanagementnetwork.org)
  • effects
  • Atienzar, F.A., Billinghurst, Z. and Depledge, M.H. (2002) 4-n-Nonylphenol and 17-β Estradiol May Induce Common DNA Effects in Developing Barnacle Larvae. (scirp.org)
  • changes
  • Most of these studies have focused on describing the frost damage mechanisms at the cellular level, trying to explain the resistance based on biochemical and physiological changes ( Guy, 1990 ). (ucv.cl)
  • identify
  • 1990) but, because members of the complex are difficult to identify by morphology, it is not known which species might be responsible for transmission in a given area. (bioline.org.br)
  • This technique took advantage of logarithmic spacing, and could be used to identify target bands ranging over a length of 20,000 nucleotides. (wikipedia.org)