• assay
  • Thus, our goal was to develop a PCR-based assay specific for small broomrape DNA and sensitive enough to detect a single small broomrape seed. (plantmanagementnetwork.org)
  • Metal content was analyzed by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay was used, and genomic DNA isolation from frozen plant samples was performed to obtain DNA fingerprints of investigated plant. (hindawi.com)
  • genome
  • In this paper, RAPD primers were used to test whether accelerated germination induced by qi energy was associated with changes in the genome. (healingtaousa.com)
  • Citrus has a small genome (1C = 0.62 pg) (Guerra, 1984), is diploid with a small number of chromosomes (n = 9) (Soost and Cameron, 1975) and is highly polymorphic. (scielo.br)
  • RAPD data of these two populations, one from the mountains of central Norway and the other from the coastal areas, were analyzed to determine whether these differences are due to divergence in the plant genome or they are merely products of environmentally induced changes. (uio.no)
  • electrophoresis
  • A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. (wikipedia.org)
  • In DNA electrophoresis, the TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA) are the usual buffers of choice. (wikipedia.org)
  • primer
  • Instead of using primer pairs as in traditional PCR, RAPD reactions use a single short primer (usually 10 bases in length) of randomly chosen sequence. (bioline.org.br)
  • For a RAPD band to be produced, the primer needs to match a binding site that is within approximately 2-3 kilobase pairs of another oppositely oriented binding site so that the single oligonucleotide can prime replication in both the forward and reverse directions. (bioline.org.br)
  • This is achieved one or two ways: 1) a DNA target is amplified at the same time via primer sets, or 2) different DNA targets are amplified independently via particular primers. (wikipedia.org)
  • 1996
  • Ioannou, Y.A. and Chen, F.W. (1996) Quantitation of DNA Fragmentation in Apoptosis. (scirp.org)
  • Savva, D. (1996) Use of DNA Fingerprinting to Detect Genotoxic Effect. (scirp.org)
  • One hundred thirty-two specimens were collected from Brazil and Northern Argentina ( Figure 1 and Table I ). Tissue collection and DNA extraction are described in Dergam (1996). (scielo.br)
  • Small
  • Spin column chromatography was used to extract DNA from small broomrape plants collected from the original infested field in Clackamas County, Oregon (DNeasy Plant Mini Kit, Qiagen, Inc., Valencia, CA). The ITS region of the nrDNA was amplified with the universal primers ITS5 and ITS4 as described (13). (plantmanagementnetwork.org)
  • different
  • Heavy metals also induce several cellular stress responses and damage to different cellular components such as membranes, proteins, and DNA. (hindawi.com)
  • thus, exposure to different metals can induce direct DNA damage and significant changes in metal content in the plant and also endogenous total antioxidants level. (hindawi.com)
  • effects
  • Atienzar, F.A., Billinghurst, Z. and Depledge, M.H. (2002) 4-n-Nonylphenol and 17-β Estradiol May Induce Common DNA Effects in Developing Barnacle Larvae. (scirp.org)
  • thus, effects of heavy metal stress on DNA damages and total antioxidants level in Urtica dioica leaves and stems were investigated. (hindawi.com)
  • changes
  • Most of these studies have focused on describing the frost damage mechanisms at the cellular level, trying to explain the resistance based on biochemical and physiological changes ( Guy, 1990 ). (ucv.cl)
  • analysis
  • Recently, advances in molecular biology have led to the development of a number of selective and sensitive assays for DNA analysis in ecogenotoxicology. (hindawi.com)
  • To address this issue, a kit for Southern Blot analysis was developed in 1990, providing the first marker to combine target DNA and probe DNA. (wikipedia.org)
  • addition
  • In addition, RAPD-PCR was investigated as a possible means for routine identifications in lieu of morphological identification keys. (bioline.org.br)
  • obtain
  • Our objective was to identify members of the Albitarsis Complex by RAPD-PCR from additional localities and obtain reared associated sibling voucher specimens for furture morphological studies. (bioline.org.br)
  • identify
  • 1990) but, because members of the complex are difficult to identify by morphology, it is not known which species might be responsible for transmission in a given area. (bioline.org.br)
  • This technique took advantage of logarithmic spacing, and could be used to identify target bands ranging over a length of 20,000 nucleotides. (wikipedia.org)
  • useful
  • The RAPD technique has been useful in studying polymorphism, identifying genes of interest, and permitting the determination of phylogenetic relationships and intraspecific diversity at a molecular genetic level. (uio.no)
  • sites
  • On the other hand, the size of the DNA pieces are based on the sites where the restriction enzyme cuts. (wikipedia.org)
  • Results
  • Results suggested that heavy metal stress influences antioxidant status and also induces DNA damages in U. dioica which may help to understand the mechanisms of metals genotoxicity. (hindawi.com)
  • this generally results in slower mobility of the DNA. (wikipedia.org)