QPCRAssaysAssayTissuesMicroarrayPrimersQuantifyAnalysisEnzymeReverseSensitivityProbe-basedMethodsMRNAReal timMeasurementDiagnosisMethodExpressionHighPotentialBackgroundReverse transcriTaqmanNuclear factor kappa-light-chain-enhancerQualitativeInfectionProteinMicroorganismsTranscriptionAllergicReal-timeRegulatoryPatientsTestFieldTargetReceptorWesternReportPlasmaSpecificSampleEffect
QPCR8
- nonetheless, until now, no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction (RT-qPCR) data. (techscience.com)
- SYBR Green Quantitative RT-qPCR Kit was used in this study and the PCR experiment was carried out in eppendorff realplex mastercycler. (ukessays.com)
- The expression levels of miR-128-3p and transforming growth factor beta receptors II (TGFBR2) were detected by quantitative polymerase chain reaction (qPCR). (degruyter.com)
- citation needed] Another method for population size estimation is a real-time polymerase chain reaction (qPCR). (wikipedia.org)
- qPCR is a molecular approach that measures the amplification of DNA over time rather than just at the end of the reaction. (wikipedia.org)
- The present study compared the airborne fungi collection performance of a two-stage cyclone sampler (active method) to the performance of the Personal Aeroallergen Sampler (passive method) using quantitative polymerase chain reaction (qPCR) assays. (cdc.gov)
- In two patients, plasma samples were negative for adenovirus by quantitative polymerase chain reaction (qPCR), but both patients were positive when retested using whole blood. (cdc.gov)
- Another type of PCR known as quantitative PCR (qPCR) measures the amount of pathogens in the sample. (medlineplus.gov)
Assays3
- Polymerase chain reaction (PCR) assays for two E. coli K-12 strains have been shown to be specific for the target strain and have sufficiently low limits of detection (less than 50 cells per PCR) for monitoring of bioprocess release. (bl.uk)
- The use of highly reproducible and accurate SYBR-based real-time polymerase chain reaction (PCR) assays instead of performing Taqman-type assays allows low-cost, high-throughput analysis of viral mRNA expression. (biomedcentral.com)
- Molecular assays (eg, quantitative polymerase chain reaction) are available but are more commonly used to detect viral reactivation. (msdmanuals.com)
Assay7
- A quantitative PCR assay, using a competitive internal standard, for one E. coli strain allows measurement of the concentration of the bacteria over a range of up to 6 orders of magnitude with a measurement error of ±0.11 logs. (bl.uk)
- The expressions of TLR4, NF-κ B and pro-inflammatory cytokines in the cortical were determined by real time polymerase chain reaction (RT-PCR), western blot, immunohistochemistry, or enzyme-linked immunosorbent assay (ELISA). (karger.com)
- Therefore, a TaqMan minor groove binder (MGB) probe-based quantitative reverse transcription polymerase chain reaction (qRT- PCR ) assay was developed to detect and quantify the CHIKV. (bvsalud.org)
- Here, we describe the development of an absolute quantitative real-time PCR assay to quantitate mRNA of the E7 oncogene from HPV 16 and HPV 18 in cervical cytobrush samples. (biomedcentral.com)
- Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)
- Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
- Plasma samples from the patients were subjected to a real-time quantitative polymerase-chain-reaction assay. (nih.gov)
Tissues4
- To demonstrate the presence of components of local RASs in humans we used the polymerase chain reaction (PCR) after reverse transcription to detect renin- angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human tissues. (jci.org)
- The levels of miR-127 in the tissues and plasma were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). (hindawi.com)
- The expression levels of VGLL3 mRNA and protein were analyzed in the freshly resected tumor and normal gastric tissues from GC patients by quantitative RT-PCR and Western blot, respectively. (researchgate.net)
- The results of a reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that miR‑320 was frequently downregulated in breast cancer tissues compared with adjacent normal tissues. (spandidos-publications.com)
Microarray1
- Quantitative real-time PCR analysis of 23 genes was used to validate the microarray results. (molvis.org)
Primers4
- After the primers bind to the complementary strand of the target DNA, it is extended by DNA polymerase using four deoxynucleotide triphosphate. (iflybio.com)
- To know in detail about designing primers for PCR reactions, click here . (iflybio.com)
- How to design primers for PCR (Polymerase Chain Reaction)? (iflybio.com)
- Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
Quantify2
- The use of real-time quantitative RT-PCR to quantify specific mRNAs allows for more rapid testing, higher sensitivity, increased simplicity, and more accuracy. (medscape.com)
- Methods: Utilizing immunohistochemistry, the localization of 5-HT and of 5-HT 1B/1D/1F receptors was examined in rat trigeminal ganglion (TG) and combined with quantitative polymerase chain reaction to quantify the level of expression for 5-HT 1B/1D/1F receptors in the TG. (lu.se)
Analysis3
- Quantitative analysis by the polymerase chain reaction. (jci.org)
- Quantitative analysis of DNA or RNA. (iflybio.com)
- In this 2007 photograph, Centers for Disease Control and Prevention (CDC) Biologist, Damien Danavall, was shown setting up a liquid handling robot, to perform a quantitative PCR (polymerase chain reaction) analysis, able to detect the presence of herpes simplex virus-2 (HSV-2). (cdc.gov)
Enzyme3
- Here, the breakthrough for DNA polymerase enzyme that could be used for PCR came with the introduction of Taq polymerase. (iflybio.com)
- During PCR, when the polymerase enzyme replicates the template that is bound with the probe, the 5′-3′ exonuclease activity of the enzyme cleaves the probe such that the reporter and quencher dyes separate and FRET no longer occurs. (iflybio.com)
- An enzyme called polymerase is added to the sample. (medlineplus.gov)
Reverse5
- The expression of Notch pathway genes in the limbal and central human corneal epithelium was compared by reverse transcription polymerase chain reaction (RT-PCR). (molvis.org)
- A TaqMan minor groove binder probe-based quantitative reverse transcription polymerase chain reaction for detection and quantification of chikungunya virus. (bvsalud.org)
- The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
- Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
- Interferon γ (IFN-γ) and interleukin (IL)-4 RNA expression was examined in T lamina propria lymphocytes by quantitative reverse transcription-polymerase chain reaction. (bmj.com)
Sensitivity1
- Quantitative RNA-polymerase chain reaction (RNA-PCR) is an extremely powerful analytical tool owing to its specificity and high level of sensitivity. (elsevierpure.com)
Probe-based1
- Therefore, primer dimers are an issue in reactions using both probe-based and SYBR Green I dye-based detection. (sigmaaldrich.com)
Methods2
- In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. (geneticsmr.com)
- Comparison of quantitative airborne fungi measurements by active and passive sampling methods. (cdc.gov)
MRNA3
- We were interested in determining if any quantitative differences existed in the expression of E7 mRNA in cells from women with normal epithelium and precancerous lesions. (biomedcentral.com)
- In order to accomplish this goal, we needed to develop a polymerase chain reaction (PCR) method that would allow us to make direct comparisons between patients in terms of amount of mRNA expressed in cervical specimens. (biomedcentral.com)
- Application of quantitative competitive polymerase chain reaction for measurements of mRNA from antioxidative enzymes in the diabetic rat retina and kidney. (lu.se)
Real tim1
- Here, we report a method of absolute quantitative real-time PCR utilizing SYBR-green fluorescence for the measurement of HPV E7 expression in cervical cytobrush specimens. (biomedcentral.com)
Diagnosis1
- Quantitative real-time polymerase chain reaction for malaria diagnosis and its use in malaria vaccine clinical trials. (ox.ac.uk)
Method2
- No analytical method currently exists that can accurately and rapidly quantitate the small quantities of DNA in RNA-PCR reaction mixtures. (elsevierpure.com)
- The use of quantitative real-time polymerase chain reaction (PCR), carried out in real-time during the clinical trials of vaccines designed to act against the liver stage of the parasite's life cycle, provides more information than the gold standard method of microscopy alone and increases both safety and accuracy. (ox.ac.uk)
Expression2
- Relative expression of transforming growth factor beta (TGF- β), myosin heavy chain beta (β-MHC), endothelial nitric oxide synthase (eNOS) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was studied. (ukessays.com)
- Changes in morphology were imaged, and changes in gene expression of CEC typical genes such as zonula occludens (ZO-1), sodium/potassium (Na/K)-ATPase, paired-like homeodomain 2 (PITX2), and collagen 8 (COL-8) were measured with real-time polymerase chain reaction. (lww.com)
High1
- Hence, Taq polymerase is resistant to high temperatures such as the denaturation temperature. (iflybio.com)
Potential2
- These data demonstrate the potential usefulness of capillary electrophoresis in a variety of quantitative PCR applications. (elsevierpure.com)
- There is low potential for the oligos of the reaction to hybridize to each other. (sigmaaldrich.com)
Background1
- The 'threshold line' is the point at which the reaction reaches a fluorescent intensity above background. (ukessays.com)
Reverse transcri1
- The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
Taqman1
- OPA1 mRNA was measured by Taqman quantitative PCR. (molvis.org)
Nuclear factor kappa-light-chain-enhancer1
- The downstream signaling of the complex proceeds inter alia via spleen associated tyrosine kinase (SYK) and the nuclear factor kappa-light-chain-enhancer (NF-κB) to induce the gene expression of pro-inflammatory cytokines, chemokines, and enzymes ( 4 , 5 ). (frontiersin.org)
Qualitative1
- Ideally, quantitative PCR is as sensitive as qualitative PCR and provides an estimate of the number of CMV genomes present in plasma. (medscape.com)
Infection2
- see Quantitative polymerase chain reaction) yield similar effectiveness in diagnosing and monitoring patients with active CMV infection. (medscape.com)
- A study of more than 3400 blood specimens from organ transplant recipients tested with CMV PCR and pp65 antigenemia found that quantitative real-time PCR for CMV DNA could be used in lieu of antigenemia for monitoring CMV infection and determining when to initiate preemptive treatment. (medscape.com)
Protein1
- 7,11 As intestinal contractility is positively correlated with the phosphorylation of the 20 kDa regulatory light chain of myosin ( p -MLC 20 ), 12 the effects of fruit bromelain on the extent of p -MLC 20 and levels of myosin light chain kinase (MLCK) protein were measured using western blotting. (rsc.org)
Microorganisms1
- Under anaerobic conditions, the microorganisms that catalyze these reactions use the solvents as electron acceptors while using a range of substrates (e.g., lactate, methanol, H 2 ) as electron donors/energy sources. (nih.gov)
Transcription4
- Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
- real-time semi-quantitative reverse transcription-polymerase chain reaction. (nih.gov)
- Decorin was examined in 18 donors and 32 patients with CP by quantitative reverse transcription polymerase chain reaction (QRT-PCR), western blotting, and immunohistochemistry of pancreatic specimens. (bmj.com)
- The expression of hsa_circRNA_101996, microRNA (miR)-143, and ten-eleven translocation (TET)-2 in GC tissues, the adjacent tissues, and cell lines were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). (jcancer.org)
Allergic1
- This study suggests that iodine directly promotes ferroptosis in activated B cells and attenuates GC reactions, leading to the alleviation of allergic symptoms. (nature.com)
Real-time1
- The use of real-time quantitative RT-PCR to quantify specific mRNAs allows for more rapid testing, higher sensitivity, increased simplicity, and more accuracy. (medscape.com)
Regulatory1
- Bromelain significantly reversed the decreased phosphorylation of the 20 kDa regulatory light chain of myosin ( p -MLC 20 ) and the expression of MLCK in constipated rats. (rsc.org)
Patients1
- The most commonly reported adverse drug reactions (≥ 25%) in IMLYGIC-treated patients were fatigue, chills, pyrexia, nausea, influenza-like illness, and injection site pain. (nih.gov)
Test1
- Other recommended test include Polymerase chain reaction (PCR) in bood and urine as well. (medscape.com)
Field1
- Quantitative polymerase chain reaction for transforming growth factor-beta applied to a field study of fish health in Chesapeake Bay tributaries. (nih.gov)
Target2
- In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
- Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
Receptor1
- Binding of the ligand to mincle leads to interaction with the signaling adapter FC receptor gamma chain (FCER1G) ( 3 ). (frontiersin.org)
Western1
- Interest in n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) has increased since it was found that their consumption in most Western populations, particularly those of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), is sub-optimal for protection against the most prevalent chronic diseases [ 5 ]. (biomedcentral.com)
Report1
- To report SUSPECTED ADVERSE REACTIONS, contact Amgen at 1-855-IMLYGIC (1-855-465-9442) or FDA at 1-800-FDA-1088 or www.fda.gov/medwatch. (nih.gov)
Plasma1
- Quantitative PCR has been used to detect plasma CMV. (medscape.com)
Specific3
- LBI suppressed OVA-specific IgE production by attenuating germinal center (GC) reaction in the draining lymph nodes. (nature.com)
- Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
- This is achieved by introducing a sample-specific barcode during the RT reaction using a 6-8 nt tag carried by either the oligo-dT or the template switch oligo (TSO). (biomedcentral.com)
Sample1
- An enzyme called polymerase is added to the sample. (medlineplus.gov)
Effect1
- The effect of bromelain on myosin light chain kinase (MLCK)-related intestinal contraction was also studied. (rsc.org)