The distal region of mouse chromosome 7 (Chr 7) contains at least ten imprinted genes, several of which are expressed from the maternal homologue in the placenta. (biomedcentral.com)
Taken together, our results demonstrate that all the distal chromosome 7 imprinted genes implicated in placental function are silenced by IC2 and Kcnq1ot1 on the paternal allele. (biomedcentral.com)
Haploid1
Haploid yeast cells expressing a SARS-CoV-2 protein fused to a high affinity GFP binding protein (GPB) are mated to the yeast GFP strain library. (microbialcell.com)
Proteins8
Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking. (microbialcell.com)
Here we discuss three innovative applications of the yeast model system to reveal functions and investigate variants of proteins encoded by the SARS-CoV-2 virus. (microbialcell.com)
Directed evolution and yeast display as described by Zahradník et al generates SARS-CoV-2 proteins with enhanced binding properties or activities. (microbialcell.com)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
Single-cell proteomics aims to characterize biological function and heterogeneity at the level of proteins in an unbiased manner. (bvsalud.org)
Our algorithm RefQuant takes advantage of this and confidently quantifies twice as many proteins per single cell compared to our previous work (Brunner et al, PMID 35226415), while our workflow currently allows routine analysis of 80 single cells per day. (bvsalud.org)
METHODS: To identify responsible surface proteins, we performed deep proteome profiling on minute numbers of dormant patient-derived xenograft (PDX) leukemia stem cells isolated from mice. (bvsalud.org)
Robust genetics, cell biology, molecular biology, and biochemistry complement deep and detailed genome annotation, a multitude of genome-scale strain collections for functional genomics, and substantial gene conservation with Metazoa to comprise a powerful model for modern biological research. (microbialcell.com)
FIGURE 1: Complementary yeast-based technologies to probe SARS-CoV-2 biology. (microbialcell.com)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
These results describe the complexity of the cell composition, regulation and organization for this organ, and serve as an important reference map for understanding human biology and disease. (stanford.edu)
Model organisms such as yeast, fruit flies, and worms have advanced the study of genomics, eukaryotic biology, and evolution. (cshlpress.org)
Abnormalities2
Factors that may place patients at increased risk of fetal genetic disorders include older maternal or paternal age, parental carrier of a balanced chromosomal rearrangement, parental aneuploidy or aneuploidymosaicism, parental carrier of a genetic disorder, prior child with a structural birth defect, previous fetus or child with autosomal trisomy or sex chromosome aneuploidy, ultrasonographic evidence of a fetus with structural abnormalities, and a high-risk genetic screening test result. (wikipedia.org)
The results show the potential for fully rescuing trans placental abnormalities that are caused by imprinting defects. (biomedcentral.com)
Assay3
Importantly, SPI is an in vivo functional assay, with a fitness readout that is amenable to quantitative analysis of protein variants and perturbation by small molecules. (microbialcell.com)
To ensure accurate quantitative assessment, the positive samples of the assay must dilute linearly and in parallel with the standard curve. (egfrsignals.com)
Splicing activity was assayed using an in vivo assay in transfected mammalian cells with rhodopsin ( RHO ) and transducin ( GNAT1 ) splicing templates. (molvis.org)
Protein5
The basis of SPI is to force an ectopic association between a query protein and each protein in the yeast proteome. (microbialcell.com)
Growth defects that result are termed SPIs and indicate that the query protein in specific contexts disrupts the normal homeostasis of the cell [2] . (microbialcell.com)
Synthetic Protein Interaction (SPI) technology by Klemm et al allows unbiased identification of toxic viral-eukaryotic protein interactions in yeast. (microbialcell.com)
Upon expression of the SARS-CoV2-GBP chimera, SPIs are identified as yeast strains having a growth defect due to the forced interaction of the viral query protein with the yeast target protein. (microbialcell.com)
The microtubules in cells that make smaller amounts of this protein were more disorganised, and these cells were unable to fuse with each other to form muscle cells. (elifesciences.org)
Melanoma2
Applying this to primary cutaneous melanoma, we discovered proteomic signatures of cells within distinct tumor microenvironments, showcasing its potential for precision oncology. (bvsalud.org)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Analyses1
Through systematic analyses, we find cell compositions that differ substantially across regions of the intestine and demonstrate the complexity of epithelial subtypes, and find that the same cell types are organized into distinct neighbourhoods and communities, highlighting distinct immunological niches that are present in the intestine. (stanford.edu)
Differentiation4
The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. (elifesciences.org)
Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)
We also map gene regulatory differences in these cells that are suggestive of a regulatory differentiation cascade, and associate intestinal disease heritability with specific cell types. (stanford.edu)
Of translational importance, molecular or pharmacological targeting of ADAM10 reduced PDX leukemia burden, cell homing to the murine bone marrow and stem cell frequency, and increased leukemia response to conventional chemotherapy in vivo. (bvsalud.org)
In this study, we used a combination of NGS, human-induced pluripotent-stem-cell-derived cardiomyocytes (iPSC-CMs) and nanopore long-read sequencing to identify the causal variant in a multi-generational pedigree of DCM. (stanford.edu)
Tissues3
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Spatial1
The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. (stanford.edu)
Molecular1
The Kopito laboratory seeks a molecular understanding of how cells maintain the fidelity of their proteomes. (stanford.edu)
Tumor2
The imprinted region on distal mouse chromosome 7 (Chr 7) shares syntenic homology with human chromosome 11p15.5, a region associated with Beckwith-Wiedemann syndrome (BWS) and Wilms tumor. (biomedcentral.com)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
Depletion2
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
Depletion of oMAP4 impairs cell elongation and cell-cell fusion. (elifesciences.org)
Chemotherapy1
Elderly less likely to benefit from simultaneous radio- & chemotherapy for lung cancer An analysis of elderly patients treated in a phase II trial of radiotherapy combined with chemotherapy in non-small cell lung cancer has shown that they were less likely to benefit than younger patients if the two treatments were given at the same time. (bionyt.dk)
Methods1
The research design is an inferential quantitative, using sampling and snowball sampling methods with total sample are 406. (preprints.org)
Mechanism1
This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
Human2
Here, we leverage a set of genome-edited human PLIN2 reporter cell lines in a series of CRISPR-Cas9 loss-of-function screens, identifying genetic modifiers that influence PLIN2 expression and post-translational stability under different metabolic conditions and in different cell types. (stanford.edu)
Organization of the human intestine at single-cell resolution. (stanford.edu)
Organization1
Here, to better understand these differences, we evaluated the organization of single cells using multiplexed imaging and single-nucleus RNA and open chromatin assays across eight different intestinal sites from nine donors. (stanford.edu)
Model2
Recently, the yeast model has demonstrated its utility in a perhaps unexpected area, that of eukaryotic virology. (microbialcell.com)
published in Microbial Cell [1] , are establishing the utility of the yeast model to address these critical questions. (microbialcell.com)
Expression1
Kcnq1ot1 expression can be abolished on the paternal chromosome by deleting IC2 (IC2KO allele). (biomedcentral.com)
Findings2
Micro-deletions or micro-duplications, such as DiGeorge syndrome and Cri-du-Chat syndrome, sex-linked disorders, such as hemophilia and Duchenne muscular dystrophy, and specific genetic mutations previously demonstrated to be present in a family or suspected based on fetal ultrasound findings, such as sickle cell disease, Tay-Sachs disease, neurofibromatosis, and cystic fibrosis. (wikipedia.org)
Handgrip strength test is good indicator of survival in lung cancer patients A simple test of handgrip strength is a good indicator of short- and long-term survival in patients with early, stage I non-small cell lung cancer (NSCLC), according to new findings to be presented at ESTRO 37 - Europe's largest radiation oncology conference. (bionyt.dk)
Target1
In practical applications, the online insect identification system is often unable to locate and identify the target pest accurately due to factors such as small target size, high similarity between species and complex backgrounds. (chinaagrisci.com)
Types2
The localization of individual cell types, cell type development trajectories and detailed cell transcriptional programs probably drive these differences in function. (stanford.edu)
Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
Samples1
We demonstrate automated and complete dimethyl labeling of bulk or single-cell samples, without losing proteomic depth. (bvsalud.org)
Muscle cells8
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. (elifesciences.org)
Skeletal muscles-which enable animals to move-are made up of large elongated muscle cells that span the entire length of the muscle. (elifesciences.org)
Cells called myoblasts elongate and fuse together at their tips to make the muscle cells. (elifesciences.org)
The filaments act as a template that helps the sarcomeres to align as the muscle cells form. (elifesciences.org)
The experiments show that the filaments progressively become more ordered as the myoblasts develop into muscle cells. (elifesciences.org)
This, in turn, allows the muscle cells to become very long. (elifesciences.org)
Making more oMAP4 alone is not sufficient to form the elongated muscle cells. (elifesciences.org)
Function1
An intriguing aspect to the association of these genes with retinal disease is that splicing occurs in every cell of the body so the genes must have a general housekeeping function, yet the disease pathology is restricted to the rod photoreceptors of the retina. (molvis.org)
Repair1
recombinational DNA repair (promoted during meiosis because homologous chromosomes pair at that time) and complementation (also known as heterosis , hybrid vigor or masking of mutations). (wikizero.com)
Release5
Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)
It was found that GSOs reversed GLU-induced reductions in cell viability and the LDH release rate, and rescued ROS accumulation. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Cold Spring Harbor Laboratory Press (CSHLP) announced the release of T-Cell Memory, available on its website in hardcover and eBook formats. (cshlpress.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
Fusion1
[7] In eukaryotes, true sexual reproduction by meiosis and cell fusion is thought to have arisen in the last eukaryotic common ancestor , possibly via several processes of varying success, and then to have persisted. (wikizero.com)
Work1
My work has been very multi-disciplinary incorporating quantitative, population, genetic, and behavioral approaches. (dukecancerinstitute.org)
Make1
The Honeycomb Game is a word-building game: make words by clicking on cells in the honeycomb below. (claylane.uk)
Made2
REFERENCES NOTE TO READERS OF THE CRITERIA DOCUMENTS While every effort has been made to present information in the criteria documents as accurately as possible without unduly delaying their publication, mistakes might have occurred and are likely to occur in the future. (inchem.org)
Keep doing this until you have made a continuous chain of touching cells, forming a word of three letters or more. (claylane.uk)
Found1
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Control1
Therefore, the next challenge is to understand how other processes-such as the selective stabilisation of some microtubules and the movement of cell materials along the microtubules-cooperate to control muscle fibre formation. (elifesciences.org)