• This product can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. (abcam.com)
  • Abcam's CytoPainter Live Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations and flow cytometric investigations of cellular functions. (abcam.com)
  • Image of CPA cells stained with CytoPainter Live Cell Labeling Kit - Green Fluorescence (ab187964) in a black wall/clear bottom 96-well plate. (abcam.com)
  • Flow cytometry uses fluorochrome-labeled cells to identify and sort them based on their size, granularity, and fluorescence intensity. (dadangoray.com)
  • Fluorescence-activated cell sorting (FACS) is a more advanced version of flow cytometry that can sort cells at high speeds based on their fluorescence intensity and other criteria. (dadangoray.com)
  • Researchers are continuously more engaged in high-parameter flow cytometry assays, analyzing twenty or more markers at high throughput, for deeper insights into the immune network of the host organism. (biolegend.com)
  • Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Attune Cytometric Software is a powerful tool designed to streamline and enhance flow cytometry data analysis. (lfdtrade.com)
  • With its user-friendly interface and advanced features, Attune Cytometric Software provides researchers with a comprehensive solution for interpreting complex data obtained from flow cytometry experiments. (lfdtrade.com)
  • Whether it's identifying rare cell populations, performing sophisticated gating strategies, or generating publication-quality plots, Attune Cytometric Software empowers researchers to unlock valuable insights and accelerate their discoveries in the field of cytometry analysis. (lfdtrade.com)
  • Attune Cytometric Software is a powerful tool used in flow cytometry analysis. (lfdtrade.com)
  • Furthermore, Attune Cytometric Software supports a wide range of file formats, making it compatible with different flow cytometry platforms. (lfdtrade.com)
  • Overall, Attune Cytometric Software empowers researchers in flow cytometry studies by offering robust analysis tools, intuitive user interface, and automation capabilities. (lfdtrade.com)
  • Cytometric software refers to computer programs and applications designed for the analysis and interpretation of data generated by cytometry experiments. (lfdtrade.com)
  • The CYBERTRACK performance is validated using two real flow cytometry datasets, which demonstrate that the population dynamics detected by CYBERTRACK are consistent with our prior knowledge of lymphocyte behavior. (biomedcentral.com)
  • Table-based software solutions are commonly used in cytometry to efficiently handle and analyze large datasets. (lfdtrade.com)
  • The deposition of Cu in lysosomes isolated from macrophages was also determined by ICP-MS. Cell viability (metabolic activity) was assessed using the Alamar Blue assay, and oxidative stress was monitored by a variety of methods including a luminescence-based assay for cellular glutathione (GSH), and flow cytometry-based detection of mitochondrial superoxide and mitochondrial membrane potential. (biomedcentral.com)
  • This means it is imperative to understand the expression level of the target, the capabilities of the cytometer, and the properties of fluorophores used for detection, especially when multiple fluorophores will be combined in a panel. (biolegend.com)
  • Fluorochrome detection refers to a technique used to identify and quantify specific biomolecules using fluorescent dyes. (dadangoray.com)
  • In summary, fluorochrome detection is a powerful technique used to identify and quantify specific biomolecules in cells and tissues. (dadangoray.com)
  • The detection of cell populations in flow cytometry data has long been dependent on "manual gating" by visual inspection. (biomedcentral.com)
  • Modern flow cytometers allow the simultaneous detection of nearly 20 protein markers per cell with a throughput of thousands of cells per second. (biomedcentral.com)
  • Flow cytometers generally provide less data than image cytometers, but have a significantly higher throughput. (wikipedia.org)
  • Progress toward a high-throughput & high-resolution pipeline to quantify nanoscale aggregates in brain tissue. (weisslab.ca)
  • Ultrahigh throughput 3D localization in an imaging flow cytometer,' Technion & Tel Aviv Biomed. (weisslab.ca)
  • This allows a time-lapse cytometer to be placed inside a conventional cell culture incubator to facilitate continuous observation of cellular processes without heat building up inside the incubator. (wikipedia.org)
  • These instruments combined a fluorescent microscope with a spectrophotometer to quantify cellular nucleic acids and their relation to cell growth and function. (wikipedia.org)
  • Flow cytometry is a widely used technology for identifying and quantifying cellular properties and cell populations by measuring expression levels of surface and intracellular proteins at the single-cell level. (biomedcentral.com)
  • During a typical flow cytometry experiment, samples are stained using fluorophore-labeled antibodies that recognize and bind specific cell surface or intracellular markers, or a variety of fluorescent chemical probes. (biolegend.com)
  • Successively the manual task of counting, using a microscope, is taken over by small automated image cytometers. (wikipedia.org)
  • Therefore, there is a great need for tools to systematically analyze time-series flow cytometry data. (biomedcentral.com)
  • However, such research is currently limited by a lack of a systematic mathematical framework to adequately model and analyze time-series flow cytometry data. (biomedcentral.com)
  • By offering seamless integration with various flow cytometers and supporting multiple file formats, this software enables scientists to efficiently analyze, visualize, and extract essential information from their experimental results. (lfdtrade.com)
  • It provides researchers with the ability to analyze and interpret complex data generated from flow cytometers. (lfdtrade.com)
  • To address this, we used flow cytometry to examine changes in the temporal expression of microglia and macrophages after SCI and intravenous morphine. (biomedcentral.com)
  • Modern flow cytometry technology has enabled the simultaneous analysis of multiple cell markers at the single-cell level, and it is widely used in a broad field of research. (biomedcentral.com)
  • Critically, as flow cytometry has advanced, the number of fluorescent readouts that can be measured simultaneously has increased, along with the sample processing speed. (biolegend.com)
  • Using flow cytometry, researchers can measure cell size and granularity as well as investigate factors including viability, cell cycle, and protein expression. (biolegend.com)
  • Time-series flow cytometry data are indispensable for investigating the dynamics of cell populations that could not be elucidated by static time-point analysis. (biomedcentral.com)
  • Our results indicate that CYBERTRACK offers better understandings of time-dependent cell population dynamics to cytometry users by systematically analyzing time-series flow cytometry data. (biomedcentral.com)
  • Time-series flow cytometry data offer information on longitudinal cell population dynamics that could not be elucidated by conventional static time-point data. (biomedcentral.com)
  • Transcripts/peptides of Notch1 and Delta-like ligand (DLL)-4 were detected in platelets isolated from human blood by RT-qPCR, Western analysis and flow cytometry. (elifesciences.org)
  • An important challenge in the analysis of flow cytometry data is the classification of individual cells into canonical cell types, that is, subset populations such as T and B cells. (biomedcentral.com)
  • The amount of this intrinsic absorption can be used to determine the physiological health of an organ-for example, whether the tissue is getting sufficient oxygenated blood flow. (nationalacademies.org)
  • however, they are not designed for time-series flow cytometry data. (biomedcentral.com)
  • We propose a simple and efficient statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), to perform clustering and cell population tracking for time-series flow cytometry data. (biomedcentral.com)
  • CYBERTRACK assumes that flow cytometry data are generated from a multivariate Gaussian mixture distribution with its mixture proportion at the current time dependent on that at a previous timepoint. (biomedcentral.com)
  • To address this problem, we propose a new statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), for the automatic clustering and tracking of a mixture proportion of cell populations in time-series flow cytometry data. (biomedcentral.com)
  • Its comprehensive features make it an indispensable asset for analyzing and interpreting data obtained from flow cytometers. (lfdtrade.com)
  • The software typically allows users to import raw data files from cytometers, apply gating strategies to identify different subpopulations within the sample, and generate graphical representations such as histograms, dot plots, or contour plots. (lfdtrade.com)
  • citation needed] Since the introduction of the digital camera, in the mid-1990s, the automation level of image cytometers has steadily increased. (wikipedia.org)
  • Cytometry, particularly flow cytometry, is a technique used in biology and medicine to measure various characteristics of cells or particles. (lfdtrade.com)
  • Cytokine expression was assessed in Tregs by flow cytometry and in skin samples and cultivated cells by immunostaining, immunoblotting, and/or qRT-PCR. (biomedcentral.com)
  • The Cellomics Arrayscan VTI instrument is an imaging tool that will help our scientists make quicker and more precise analyses, capturing, for instance, changes in cell size, shape and intensity. (lu.se)
  • Our flow cytometry studies showed that 3 consecutive days of morphine administration after an SCI significantly increased the number of microglia and macrophages around the lesion. (biomedcentral.com)
  • This allows biomolecules to be identified and quantified in cells or tissues, making it an essential tool for many biological studies. (dadangoray.com)
  • When tandem dyes are incorporated into a flow cytometry experiment, it is possible to generate multiple readouts from the same laser. (biolegend.com)
  • In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. (wikipedia.org)
  • This has led to the commercial availability of automated image cytometers, ranging from simple cell counters to sophisticated high-content screening systems. (wikipedia.org)
  • citation needed] Cell sorters are flow cytometers capable of sorting cells according to their characteristics. (wikipedia.org)
  • The early history of cytometry is closely associated with the development of the blood cell counting. (wikipedia.org)
  • At both high (x ray) and low (radio frequency [RF]) energies the body is mostly transparent, thus allowing the non-invasive imaging of the internal structure of organs and bones. (nationalacademies.org)
  • Flow cytometry, which first began to develop in the 1950s, is a technology for characterizing the phenotype and functionality of individual cells within a heterogeneous population. (biolegend.com)
  • Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. (wikipedia.org)
  • We found that wild type (WT) C57BL/6 mice, subjected to hind limb ischemia (HLI) and supplemented with carnosine (1g/L) in drinking water, had improved blood flow recovery and limb function, enhanced revascularization and regeneration of myocytes compared with HLI mice placed on water alone. (frontiersin.org)
  • Similarly pretreatment of WT C57/Bl6 mice with carnosine showed enhanced blood flow in the ischemic limb following HLI surgery. (frontiersin.org)
  • Flow cytometers operate by aligning single cells using flow techniques. (wikipedia.org)
  • Oocysts from 21 human stool specimens were separated from other stool components using discontinuous density gradient centrifugation and purified further by flow cytometry. (cdc.gov)