Putative human protein serine threonine kinases. Medical search. Frequent questions

The method, named Predikin, identifies key conserved substrate-determining residues in the kinase catalytic domain that contact the substrate in the region of the phosphorylation site and so determine the sequence surrounding the phosphorylation site. (biomedcentral.com)
Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)
The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
All of the conserved aa residues of the mouse pim-1 gene, which are homologous to known protein kinases are conserved in the predicted human protein. (drugbank.com)
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors. (lookformedical.com)
is a kinase enzyme , in particular a protein kinase , that phosphorylates the OH group of the amino-acid residues serine or threonine , which have similar side chains. (cloudfront.net)
While serine/threonine kinases all phosphorylate serine or threonine residues in their substrates, they select specific residues to phosphorylate on the basis of residues that flank the phosphoacceptor site, which together comprise the consensus sequence . (cloudfront.net)
Since the consensus sequence residues of a target substrate only make contact with several key amino acids within the catalytic cleft of the kinase (usually through hydrophobic forces and ionic bonds ), a kinase is usually not specific to a single substrate, but instead can phosphorylate a whole 'substrate family' which share common recognition sequences. (cloudfront.net)
The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. (rupress.org)
PTKs catalyze the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates. (umbc.edu)
Putative target residues can be predicted by searching for matches to the consensus for a particular kinase. (biomedcentral.com)
Kinases enzymatically catalyse the transfer of a phosphate group from a donor, such as ADP or ATP, to a protein at serine, threonine or tyrosine residues. (ddw-online.com)
IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. (thermofisher.com)
Predicated on series evaluation and a 3D style of pUL97, the viral kinase displays structural similarity to CDK2 in the catalytic middle and in functionally essential residues from the ATP binding site [16]. (niepokorny.org)
The forming of pUL97 oligomers and ADOS dimers is dependant on a self-interaction domain, located within proteins 231-280, that facilitates solid autophosphorylation taking place at N-terminal residues [35 generally,36,37]. (niepokorny.org)

This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. (wikipedia.org)
Some family members, such as latrophilins and gliomedin, are membrane-bound proteins containing the olfactomedin domain in the extracellular N-terminal region, while the intracellular C-terminal domain of these proteins is essential for the transduction of extracellular signals to the intracellular signaling pathway. (nih.gov)

Serine/threonine-protein kinase N1 is an enzyme that in humans is encoded by the PKN1 gene. (wikipedia.org)
The protein encoded by this gene belongs to the protein kinase C superfamily. (wikipedia.org)
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. (origene.com)
The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. (antibodypedia.com)
A full-length (6.1-kb) human genomic pim-1 gene, together with its immediate 5'-upstream promoter sequence (Ppim) was isolated and sequenced. (drugbank.com)
The human pim-1 gene shares an overall nucleotide (nt) sequence identity of 53% with the previously reported murine pim-1 gene. (drugbank.com)
The gene codes for a predicted Pim-1 protein of 313 amino acids (aa) with an Mr of 35,690 and a pI of 5.7. (drugbank.com)
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. (senescence.info)
Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. (senescence.info)
Recently, reduced expression of CDK10 has been observed in several cancerous human tissue, suggesting that CDK10 is a tumor suppressor gene. (jcancer.org)
The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene. (lookformedical.com)
In this report, we describe a testis zinc finger protein (Tzfp) that binds to the upstream flanking sequence of the Aie1 gene. (lookformedical.com)
The gene codes for a protein kinase. (cloudfront.net)
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium- mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. (string-db.org)
A variety of approaches, including biochemical purification, gene isolation by homology, and genetic screens, have been successfully used for the identification of putative protein kinases and phosphatases. (rupress.org)
The TEL gene is a frequent fusion partner of other tyr kinase oncogenes, including Tel/Abl, Tel/PDGFRbeta, and Tel/Jak2, found in patients with leukemia and myeloproliferative disorders. (umbc.edu)
Protein kinases are one of the largest and most influential of gene families: constituting some 2% of the proteome, they regulate almost all biochemical pathways and may phosphorylate up to 30% of the proteome. (wormbook.org)
Protein kinases are critical to cellular signalling and post-translational gene regulation, but their biological substrates are difficult to identify. (biomedcentral.com)
The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. (affbiotech.com)
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)

Protein kinases, the enzymes responsible for protein phosphorylation, make up almost 2% of protein-encoding genes in the human genome [ 1 ] and an estimated 30-50% of human proteins are phosphorylated [ 2 ]. (biomedcentral.com)
The deduced aa sequence of the human Pim-1 has 94% identity with the murine Pim-1 whereas the nt sequences of the two genes are 88% identical. (drugbank.com)
A curated database of candidate human ageing-related genes and genes associated with longevity and/or ageing in model organisms. (senescence.info)
Database of human genes associated with cellular senescence. (senescence.info)
While the catalytic domain of these kinases is highly conserved , the sequence variation that is observed in the kinome (the subset of genes in the genome that encode kinases) provides for recognition of distinct substrates. (cloudfront.net)
Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has been reported. (bgu.ac.il)
Degenerate oligonucleotide primers corresponding to conserved sequence motifs, which distinguish the PKC family from other protein kinases, were employed in polymerase chain reactions (PCR) to amplify partial core sequences of putative PKC genes from a human peripheral blood lymphocyte-derived cDNA library. (bgu.ac.il)
This domain occurred 415 times on human genes ( 944 proteins). (umbc.edu)
To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
Such radiations include genes involved in spermatogenesis, chemosensation, Wnt signaling and FGF receptor-like kinases. (wormbook.org)
Protein kinases constitute one of the largest and most important of protein families, accounting for ~2% of genes in a variety of eukaryotic genomes. (wormbook.org)
We identified 438 protein kinase genes, including 20 atypical kinases, and an additional 25 kinase fragments or pseudogenes. (wormbook.org)
We also show that human CDK targets are enriched for proteins that contain clustered consensus matches and, by searching human cell cycle genes, we predict several putative CDK targets, including the human orthologs of Schizosaccharomyces pombe CDC5 (CDC5L) and S. cerevisiae Cdc20p (CDC20). (biomedcentral.com)
A search of human gut metagenomes for circular contigs encoding phage hallmark genes resulted in the identification of 3738 apparently complete phage genomes that represent 451 putative genera. (biomedcentral.com)
We study genes, proteins and signaling pathways that might be essential for RGC and optic nerve development, function, survival, and regeneration. (nih.gov)
We are particularly interested in genes encoding olfactomedin domain-containing proteins. (nih.gov)
Several genes encoding olfactomedin domain-containing proteins are expressed in the eye. (nih.gov)

AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. (senescence.info)
Glioblastoma (GBM), a very aggressive and incurable tumor, often results from constitutive activation of EGFR (epidermal growth factor receptor) and of phosphoinositide 3-kinase (PI3K). (sdbonline.org)
The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. (lookformedical.com)
Types include those acting directly as membrane-bound receptors ( Receptor protein serine/threonine kinase ) and intracellular kinases participating in Signal transduction . (cloudfront.net)
Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. (thermofisher.com)
Transient receptor potential canonical 6 (TRPC6) is a cation selective, DAG-regulated, Ca 2+ -permeable channel activated by the agonists of G q -protein-coupled heptahelical receptors. (mdpi.com)

Catalytic domain of the Protein Tyrosine Kinase, Abelson kinase. (umbc.edu)
Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. (embl.de)
A further 91 subfamilies were found in all three metazoan kinomes, including the tyrosine kinase (TK) group and the TKL group, which mediate much of the complexity of intercellular signal transduction. (wormbook.org)

A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. (lookformedical.com)

Although Doublecortin does not contain a kinase domain, it is homologous to the amino terminus of a predicted kinase protein, indicating a likely role in signal transduction. (nih.gov)
The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. (wikipedia.org)
Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
By phosphorylating substrate proteins, kinases modify the activity, location and affinities of up to 30% of all cellular proteins, and direct most cellular processes, particularly in signal transduction and co-ordination of complex pathways. (wormbook.org)
Mathematical modeling of signal transduction networks has previously been used to map out thermodynamical using rate equations is increasingly attracting attention as a properties of protein-folding models (6,7). (lu.se)

A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. (lookformedical.com)
Many of these pathways are highly conserved, and 53 distinct kinase functions and subfamilies appear to have been conserved between yeasts, nematodes, insects and vertebrates, with a further 91 subfamilies of kinases being seen throughout metazoan genomes. (wormbook.org)

The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. (wikipedia.org)
These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). (senescence.info)
This study reports that LKB1 binds to Phosphoinositide-dependent kinase (PDK1) by a conserved binding motif. (sdbonline.org)
Human homologs of the AKT8 oncogenic protein were identified in 1987.By 1995 it had been found that Akt kinases function as mitogen-activated kinases downstream from cell surface receptors that activate phosphoinositide 3-kinase . (cloudfront.net)
The PTKc family is part of a larger superfamily that includes the catalytic domains of other kinases such as protein serine/threonine kinases, RIO kinases, and phosphoinositide 3-kinase (PI3K). (umbc.edu)

To understand the role of autophagy in the pathogenesis of glial tumors in vivo, an established Drosophila melanogaster model of glioma was used based on overexpression in larval glial cells of an active human EGFR and of the PI3K homolog Pi3K92E /Dp110. (sdbonline.org)

Many kinases are inhibited by a pseudosubstrate that binds to the kinase like a real substrate but lacks the amino acid to be phosphorylated. (cloudfront.net)
Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. (cancerindex.org)

These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. (senescence.info)
The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)

Aurora kinase c. (lookformedical.com)
Aurora kinase C is a chromosomal passenger protein that interacts with aurora kinase B in the regulation of MITOSIS. (lookformedical.com)
An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. (lookformedical.com)

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. (antibodies-online.com)
It phosphorylates and activates several downstream kinases, including AMP-dependent kinase, AMPK . (sdbonline.org)
These were formerly included in EC number '2.7.1.37', which was a general EC number for any enzyme that phosphorylates proteins while converting ATP to ADP (i.e. (cloudfront.net)

We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
The relative contribution of substrate recruitment and peptide specificity to protein kinase substrate specificity varies between protein kinases. (biomedcentral.com)
The prediction of peptide specificity is therefore the basis for most of the available computational methods aimed at predicting substrates of protein kinases. (biomedcentral.com)
Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. (string-db.org)
Because of the low specificity of the CDK consensus, however, databases of protein sequences are expected to contain large numbers of matches by chance. (biomedcentral.com)
Structures of Ric-8B in complex with Galpha protein folding clients reveal isoform specificity mechanisms. (stanford.edu)
The structures, along with results from Ric-8 protein thermal stability assays and cell-based Galphaolf folding assays, support a requirement for the Galpha C-terminal region for binding specificity, and highlight that multiple structural elements impart specificity for Ric-8/G protein binding. (stanford.edu)

Primary structure of the putative human oncogene, pim-1. (drugbank.com)
Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)
NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)

Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. (thermofisher.com)

Within the cDNA, open reading frames (ORF) were defined encoding proteins of 367 and 358 amino acids respectively, as well as conserved kinase domains typical of the superfamily of Ser/Thr kinases. (elsevierpure.com)

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. (string-db.org)

Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. (embl.de)
The human and mouse homologues of TSSK 1 and TSSK 2, together with TSSK 3 and SSTK/FKSG82, constitute a kinase subfamily closely related to the calmodulin kinases and SNF/nim 1 kinase subfamilies. (elsevierpure.com)

Despite representing one of the largest classes of therapeutic targets, most inactive-state G protein-coupled receptors (GPCRs) have remained inaccessible for cryo-EM because their small size and membrane-embedded nature impedes projection alignment for high-resolution map reconstructions. (stanford.edu)
Adhesion G-protein-coupled receptors (aGPCRs) are characterized by the presence of auto-proteolysing extracellular regions that are involved in cell-cell and cell-extracellular matrix interactions1. (stanford.edu)

The new features significantly enhance the ability of Predikin to analyse protein kinases and their substrates. (biomedcentral.com)
Protein kinases and their substrates regulate essentially all cellular processes through complex regulatory networks, in which phosphorylated proteins act as switches that tune the response of the cell to environmental stimuli. (biomedcentral.com)
In general, a protein kinase acts on a discrete set of substrates to ensure that signalling fidelity is maintained. (biomedcentral.com)
Thus, the two substrates of this enzyme are ATP and a protein , whereas its two products are ADP and phosphoprotein . (cloudfront.net)
Furthermore to people indirect results on cell routine legislation, the viral proteins kinase pUL97 straight cross-talks with CDKs since it mimics CDKs in phosphorylating partly-identical substrates and evidently possesses commonalities in protein framework and functionality. (niepokorny.org)

In enzymology , the term serine/threonine protein kinase describes a class of enzymes in the family of transferases , that transfer phosphates to the oxygen atom of a serine or threonine side chain in proteins . (cloudfront.net)
Kinase enzymes are involved in many types of cellular signalling and are implicated in several disease processes. (ddw-online.com)

Protein phosphorylation can regulate enzyme function, mediate protein-protein interactions, alter subcellular localization, and control protein stability. (rupress.org)
A relatively well understood example is the network that controls progression of the cell cycle, where cyclin-dependent kinases (CDKs) couple with various cyclins over the cell cycle to regulate critical processes [ 2 - 4 ]. (biomedcentral.com)
Mammalian Ric-8 proteins act as chaperones to regulate the cellular abundance of heterotrimeric G protein alpha subunits. (stanford.edu)

The Ppim has no TATA- or CAAT-box sequences but does contain a number of nt sequences similar to the putative binding sites of several presumptive transcription factors. (drugbank.com)
The deduced primary structure contains consensus sequences characteristic of protein kinase catalytic domains and, based on its amino acid sequence and domain structure, is a member of the PKC family. (bgu.ac.il)
All sequences and supporting data are available at http://kinase.com , and all but 8 sequences are now identical to wormpep (v. 141) sequences. (wormbook.org)
Therefore, many of the matches in protein sequences are likely to be false-positive predictions. (biomedcentral.com)

Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
Distribution of 212 kinase subfamilies throughout four kinomes: the yeast Saccharomyces cerevisiae , the nematode worm Caenorhabditis elegans , the fruit fly Drosophila melanogaster and human. (wormbook.org)

Abl (or c-Abl) is a ubiquitously-expressed cytoplasmic (or nonreceptor) tyr kinase that contains SH3, SH2, and tyr kinase domains in its N-terminal region, as well as nuclear localization motifs, a putative DNA-binding domain, and F- and G-actin binding domains in its C-terminal tail. (umbc.edu)

AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. (senescence.info)

It consists of six exons and five introns and contains a protein-coding region that is identical in nt sequence to a full-length human pim-1 cDNA. (drugbank.com)
The complete cDNA sequence was determined by anchored PCR cloning and sequencing the entire coding sequence, using cDNA derived from a human leukemic T cell line (Jurkat). (bgu.ac.il)
Most protein kinases share a common ePK (eukaryotic protein kinase) catalytic domain, and can be identified by sequence similarity with Blast or profile hidden Markov models (HMMs). (wormbook.org)
The remaining atypical protein kinases (aPK) belong to several families, some of which have structural, but not sequence similarity to ePKs. (wormbook.org)
The classification is based on sequence similarity within the kinase domain, the presence of additional domains, known biological functions, and conservation across divergent genomes. (wormbook.org)
Our data suggest that regulatory modules may exist in protein sequence as clusters of short sequence motifs. (biomedcentral.com)
instead of attempting to predict individual phosphorylation sites, we search for proteins that contain high densities of strong and weak consensus matches that are closely spaced in the primary amino acid sequence. (biomedcentral.com)
Together with advanced approaches for sequence assembly and powerful methods of sequence analysis, this revised framework offers the opportunity to discover and classify unknown phage taxa in the human gut. (biomedcentral.com)

These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. (senescence.info)
Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
Pleckstrin is one of the rare proteins to contains two PH domains. (embl.de)

Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. (origene.com)
In human, aurora-A has focused a lot of attention, since its overexpression has been found to be correlated with the grade of various solid tumours. (lookformedical.com)
Ectopic kinase overexpression in any culture cell line leads to polyploidy and centrosome amplification. (lookformedical.com)

An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. (thermofisher.com)
Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. (thermofisher.com)

We show that cyclin-dependent kinase (CDK) consensus motifs are frequently clustered in CDK substrate proteins. (biomedcentral.com)

We demonstrated that Olfm1 interacts with several proteins essential for neuronal and synaptic activity. (nih.gov)
We herein demonstrated that CADM1 interacts with Hippo pathway core kinases and enhances the phosphorylation of YAP1, and also that the membranous co-expression of CADM1 and LATS2 predicts a favorable prognosis in lung adenocarcinoma. (cancerindex.org)
Furthermore, pyrin interacts with proline-serine-threonine phosphatase-interacting protein (PSTPIP1), also known as CD2-binding protein 1 (CD2BP1), which is a tyrosine-phosphorylated protein involved in cytoskeletal organization and thereby involved in immunologic cellular interactions. (medscape.com)

Serine/threonine kinases play a role in the regulation of cell proliferation, programmed cell death ( apoptosis ), cell differentiation, and embryonic development. (cloudfront.net)
Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
Cryopyrin (also called NALP3, PYPAF1, or NACHT, leucine-rich repeat [LRR], and PYD domains-containing protein 3) is a member of the APAF-1/NOD1-like protein family, which regulates apoptosis and inflammation. (medscape.com)
In healthy individuals, cryopyrin and apoptosis-associated speck-like protein (ASC) interact via the oligomerization of ASC to induce both apoptosis and activate nuclear factor (NF)-kappa B. (medscape.com)

Notably, the next SD includes an invariant lysine residue (K355), the substitute mutation which resulted in an entire lack of kinase activity [16,30,31]. (niepokorny.org)

However, despite impressive advances in the technologies for identifying, validating and in vitro screening of compounds for their effect on individual putative targets, it is unclear whether this has improved the rate of new drugs entering the marketplace (1). (ddw-online.com)
This well defined function, combined with the fact that kinases are usually amenable to the development of in vitro screens for the identification of compounds that inhibit or enhance kinase activity, has made them the subject of many target-based drug discovery efforts. (ddw-online.com)

Although the human gut offers a rich source of phage genomic diversity, the virome so far has been explored to a much lesser extent than the whole community (metagenome), composed of viruses, bacteria, and archaea. (biomedcentral.com)

LKB1 itself is a constitutively active kinase, which is regulated by posttranslational modifications and direct binding to phospholipids of the plasma membrane. (sdbonline.org)
The resulting BCR-Abl fusion protein is constitutively active and associates into tetramers, resulting in a hyperactive kinase sending a continuous signal. (umbc.edu)

Mammalian aurora-A belongs to a multigenic family of mitotic serine/threonine kinases comprising two other members: aurora-B and aurora-C. In this review we will focus on aurora-A that starts to localize to centrosomes only in S phase as soon as centrioles have been duplicated, the protein is then degraded in early G1. (lookformedical.com)

the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. (liverpool.ac.uk)

STP, serine/threonine protein phosphatase. (rupress.org)

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. (string-db.org)
May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. (string-db.org)

Protein phosphorylation in particular plays a significant role in a wide range of cellular processes and is a very important posttranslational modification . (cloudfront.net)
Levels of cellular protein phosphorylation are modulated both by protein kinases and phosphatases. (rupress.org)
To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. (rupress.org)
Since kinases perform such a variety of distinct basic cellular functions, it is not surprising to see that 53 subfamilies and functions are present in all four kinomes ( Figure 1 ). (wormbook.org)
Protein kinases are ubiquitous components of cellular signalling networks [ 1 ]. (biomedcentral.com)

TA binding stabilizes breaks in the middle of transmembrane helices 6 and 7 that facilitate aGPCR coupling and activation of heterotrimeric G proteins. (stanford.edu)

In contrast, the expression levels in the brain and testes are considerably lower, and no transcripts were detected in several human carcinoma cell lines. (bgu.ac.il)
At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
Expression of the serine/threonine kinase hSGK1 in chronic viral hepatitis. (lshtm.ac.uk)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. (elsevierpure.com)
Similar to the mouse, tissue expression by northern and dot blot analysis revealed that human TSSK 1 and 2 messages are expressed exclusively in the testis. (elsevierpure.com)
Dive into the research topics of 'Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. (elsevierpure.com)
We demonstrated that hypoxia preconditioning enhanced the expression of Pim-1 kinase in MSCs in a time-dependent manner, as detected by quantitative PCR (qPCR) and western blot. (biomedcentral.com)

Analysis of phage genomes identified in whole-community human gut metagenomes resulted in the delineation of at least three new candidate families of Caudovirales and revealed diverse putative mechanisms underlying phage-host interactions in the human gut. (biomedcentral.com)
Thus, circularity can be used as one feature to identify putative complete phage genomes in viromes and metagenomes. (biomedcentral.com)

They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)

Cyclin-dependent kinase (CDK) 10, is reported to play an essential role in the progression from the G2 to M phase of the cell cycle. (jcancer.org)
Endogenous regulation of the cell cycle depends on phosphorylation and dephosphorylation of the cyclin- cyclin-dependent kinase (CDK)-cyclin-dependent kinase inhibitor (CDKI) pathway. (jcancer.org)
Current scientific trials are looking into cyclin-dependent kinase (CDK) inhibitors, such as for example roscovitine, an inhibitor of CDK1, -2, -5, -7, and -9, that lowers viral DNA synthesis, creation of late protein and infectious pathogen particles [4]. (niepokorny.org)
CDKs are cyclin-dependent serine-/threonine-specific proteins kinases, the experience of which depends upon cyclin binding. (niepokorny.org)

AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. (senescence.info)
Domain commonly found in eukaryotic signalling proteins. (embl.de)

We investigated whether impaired regulation of bone morphogenetic protein-2 (BMP-2) via epigenetic pathways is associated with renal cell carcinoma (RCC) pathogenesis. (oncotarget.com)
Seems to act as a scaffold protein in multiple signaling pathways. (string-db.org)
Through extensive biochemical and genetic analysis, we now know that pathways are not simply switched on with kinases and off with phosphatases. (rupress.org)

High-resolution structures of GPR56 and LPHN3 in their active, G-protein-coupled states, reveal that after dissociation of the extracellular region, the decrypted TA peptides engage the seven-transmembrane domain core with a notable conservation of interactions that also involve extracellular loop 2. (stanford.edu)

This makes kinase signaling particularly amenable to comparative studies, and kinase activity a particularly good readout of the physiological state of any cell. (wormbook.org)

Defects in these networks result in a variety of disease states making protein kinases important targets for drug design [ 3 ]. (biomedcentral.com)
We show that it is possible to predict proteins that are likely to be targets of CDKs in S. cerevisiae by searching for proteins that contain clustered matches to the CDK consensus. (biomedcentral.com)

Anatomy18

Organisms8

Diseases2

Chemicals and Drugs147

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes54

Disciplines and Occupations1

Information Science4

Residues19

Signaling pathway2

Gene21

Genes17

Receptor7

Tyrosine kinase3

Family of serine1

Signal transduction5

Highly conserved2

Phosphoinositide5

Homolog1

Binds3

Including cell proliferation2

Aurora kinase4

Phosphorylates3

Specificity7

Oncogene3

Intracellular1

Superfamily1

Calmodulin-dependent protein1

Subfamily2

Receptors2

Substrates5

Enzymes2

Regulate3

Sequences4

Saccharomyces2

Cytoplasmic1

Phosphatidylinositol1

Sequence8

Pleckstrin4

Overexpression3

Casein2

Substrate proteins1

Interacts3

Apoptosis4

Mutation1

Compounds2

Genomic1

Constitutively active2

Mitotic1

Homologous1

Phosphatase1

Neuronal2

Cellular5

Heterotrimeric1

Expression8

Genomes2

Downstream1

Cyclin4

Eukaryotic2

Pathways3

Interactions1

Amenable1

Targets2