Purification protein binding. Medical search. Frequent questions

The successfully synthesized and characterized photoaffinity trehalose-functionalized nanoprobe was employed in optimizing the protein receptor's capture, isolation, and identification. (uml.edu)
When the recommended protocol is followed, each isolation requires the following volumes of buffer: 0.75 ml of Lysis/Binding Buffer, 0.75 ml of Wash Buffer and 0.4 ml of Elution Buffer. (neb.com)
Identification and isolation of damage-recognition protein complexes from zebrafish(Danio rerio) early embryos. (ncl.edu.tw)

The Cobalt Chelating Resin is specifically designed for the purification of proteins that associate with Cobalt ions, including 6x histidine tagged proteins. (gbiosciences.com)
Immobilized metal affinity chromatography (IMAC) resin utilizing cobalt (Co 2+ ) for the purification of 6x histidine tagged proteins. (gbiosciences.com)
The chromatography medium provides very high binding capacity for histidine-tagged proteins and shows negligible leakage of Ni 2+ ions. (sigmaaldrich.com)
Ni Sepharose High Performance precharged with Ni2+ for high-performance puriﬁcation of histidine-tagged proteins. (sigmaaldrich.com)
To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)

The bifunctional fusion protein was expressed in Escherichia coli, recovered on a cellulose column and purified by elution at alkaline pH, ProtA-CBD was used to purify IgG from rabbit serum and its ability to bind IgC from different sources was determined. (huji.ac.il)
Scientists working with recombinant proteins expressed in Escherichia coli probably use at least one liquid chromatography technique to purify their protein of interest. (the-scientist.com)
Representatives of all three families were cloned and the proteins were expressed heterologously in escherichia coli. (europa.eu)

100mg of a 50kDa 6XHis tagged proteins to a ml of resin. (gbiosciences.com)

Labeling proteins with the small Strep- tag II enables efficient, standardized purification of recombinant proteins expressed in prokaryotic and eukaryotic systems, and delivers highly pure proteins. (qiagen.com)

Eukaryotic High-Mobility Group B (HMGB) proteins alter DNA elasticity while facilitating transcription, replication and DNA repair. (shengsci.com)
There are two major P450 classes in terms of the native RP systems, namely, the prokaryotic Class I P450 consisting of three stand-alone components (redoxin reductase/redoxin/P450) that are all cytosolically soluble proteins and the two-component eukaryotic Class II P450 comprised of P450 and cytochrome P450 reductase (CPR), both of which are membrane-bound proteins. (nature.com)
Compared to the membrane-bound eukaryotic P450s, bacterial P450s in soluble form have better application value and potential. (nature.com)
The DNA damage-recognition problem in human and other eukaryotic cell: the XPA damage binding protein. (ncl.edu.tw)

and (ii) CBD-fusion proteins bind directly to cellulose at high density. (huji.ac.il)

But liquid chromatography frequently requires a considerable amount of optimization, and usually involves several different chromatographic steps to rid the sample of contaminants.1 The ideal solution would be to create a resin that is completely specific to the target protein, enabling one-step purification. (the-scientist.com)
The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
Size exclusion chromatography showed that the native protein formed a dimer, whereas the His-tagged protein was a monomer. (europa.eu)
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. (gbiosciences.com)
Strep- Tactin is available as Superflow chromatography resin for larger scale purification (up to 9 mg/ml resin). (qiagen.com)
Strep- Tactin resin is also available in prefilled cartridges for automated purification on liquid chromatography systems. (qiagen.com)
Strep -tagged proteins can be purified using batch or gravity-flow methods, or FPLC-compatible columns can be filled with Strep -Tactin Superflow Plus for purification on liquid chromatography systems. (qiagen.com)
Strep -Tactin Superflow Plus is available in 5 ml cartridges for automated purification on liquid chromatography systems such as the FPLC, ÄkTA, and BioLogic systems, or manual purification using a syringe. (qiagen.com)
The ability of protein A affinity chromatography to achieve 95% purity in one simple step was the runway that got recombinant immunotherapy off the ground and made it available to millions. (bioprocessintl.com)

Characterization of DNA recognition by the human UV-damaged DNA-binding protein. (ncl.edu.tw)

The miniF plasmid C protein: sequence, purification and DNA binding. (shengsci.com)
The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. (shengsci.com)
in others, the target protein is expressed from a plasmid that encodes for an "affinity tag" specific to a particular ligand. (the-scientist.com)
Plasmid preparations can be contaminated with host DNA, RNA, and proteins. (bioprocessintl.com)
All the fluorinated compounds are unable to transfect RD-4 cells, if used alone, but they are all able to deliver a plasmid carrying an enhanced green fluorescent protein (EGFP) expression cassette, when co-formulated with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE) in a 1:2 ratio. (bvsalud.org)

When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
Because poly(A) addition by Poly(A) Polymerase is distributive, the tailed mRNA is heterogeneous in length, gel purification of tailed mRNA is not recommended. (neb.com)
Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)

Cells continuously monitor protein synthesis to prevent accumulation of aberrant polypeptides. (shengsci.com)
A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)

Importantly, all of these enzymes catalyzed N -glycosylation reactions in vitro with no purification or processing needed. (biorxiv.org)
METHODS: Hybridomas were generated from Balb/C mice that had been immunized with autologous serum proteins reacted with MDI in vitro. (cdc.gov)
Some of the mAbs tested enhanced the in vitro light chain activity of BoNT/B, suggesting that BoNT/B may undergo conformational change upon binding some mAbs. (cdc.gov)

Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor. (qiagen.com)
Protein Expression and Purification, molecular modelling, and molecular dynamics simulation. (umassmed.edu)
Protein Expression and Purification, Computational Modeling, Molecular Dynamic Simulations, X-ray Crystallography, Enzyme Activity and Enzyme Inhibition Assays, Structure Aided Drug Design. (umassmed.edu)

Conventional coimmunoprecipitation (coIP) and pull-down assays were used to test for stable protein complexes. (jneurosci.org)
Following purification, differences in the relative affinities of the mAbs was determined through competitive binding assays, and suggested differences in epitope recognition. (cdc.gov)

The binding efficiency was dependent on the sequence, relative orientation, and spacing of the repeated motifs of response elements. (nih.gov)
citation needed] The proteins encoded by this gene are transcription factors that bind to a sequence in the promoter of different genes, called sterol regulatory element-1 (SRE1). (wikipedia.org)
Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
Sre1p specifically bound DNA containing the 5'-(G/A)ATC(T/A)GATAA-3' sequence, and that binding was both iron- and zinc-dependent. (nih.gov)
Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (jci.org)
Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). (iucr.org)

Cytochrome P450 enzymes (P450s or CYPs) are a superfamily of heme-thiolate proteins widespread in all kingdoms of life 1 . (nature.com)
In the structures with the inhibitor bound at Q(P), no density is observed at Q(D), which suggests that the occupancy of this site can vary and argues against a structurally obligatory role for quinol binding to Q(D). A comparison of the Q(P) site of the E. coli enzyme with quinone-binding sites in other respiratory enzymes shows that an acidic residue is structurally conserved. (rcsb.org)
Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. (gbiosciences.com)
Utilizing computational methodologies, including molecular modeling and conventional molecular dynamics simulations to understand the relationship between the three-dimensional structures, dynamics and the biological functions of DNA-binding enzymes and proteins central to Type I Diabetes. (umassmed.edu)

Muscleblind-like proteins (MBNLs) are regulators of RNA metabolism. (shengsci.com)

Mutations in the conserved cysteine residues in the cysteine-rich region led to a decrease in bound iron. (nih.gov)
This resin binds to six histidine residues (6x His), a common tag used in protein purification. (gbiosciences.com)

Extra column washes before elution may help in minimizing binding reagent carry over. (neb.com)
Specific binding/wash and elution buffers are available. (gbiosciences.com)
Strep- Tactin Superflow Plus allows highly efficient purification of proteins (see figures Efficient purification of Strep -tagged GFP. GFP was expressed in 60 ml E. coli culture and a 5 ml aliquot was applied to Strep -Tactin Superflow Plus. Protein was eluted using 2.5 mM desthiobiotin, giving a total protein yield of 3.1mg. C: Cleared lysate; E: Elution fractions; F: Flow-through; M: Markers; W: Wash. "> Efficient purification of Strep-tagged GFP with Strep-Tactin Superflow Plus and Highly specific detection of Strep-tagged proteins in any expression system. Purified Strep -tagged thioredoxin (10 ng) was mixed with crude cell lysate from mammalian (NIH-3T3), insect (Sf9), and bacterial ( E. coli ) cells (each 10 µg total protein). After electrophoresis and western transfer, the Strep -tagged protein was detected using the Strep -tag Antibody and chemiluminescent detection. "> Highly specific detection of Strep-tagged proteins in any expression system ). (qiagen.com)
After washing, proteins are eluted using low concentrations of biotin (the natural ligand of streptavidin) or desthiobiotin (a stable, reversibly binding biotin analog, see figure Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin. "> Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin ). (qiagen.com)
Bring all three buffers (Lysis/Binding, Wash and Elution Buffers) to a final concentration of 8M Urea or 6M Guanidine. (neb.com)
500 mM imidazole in the elution buffer ensures complete elution of the target protein. (sigmaaldrich.com)
For linear gradient elution, a shallow gradient, over 20 column volumes, may separate proteins with similar binding strengths. (sigmaaldrich.com)
After elution, regenerate the column by washing it with 5 to 10 column volumes of binding buffer. (sigmaaldrich.com)

The nanoprobe could magnetically isolate the trehalose-bound protein complex without additional affinity purification. (uml.edu)
Affinity purification of proteins with a 6x His tag. (gbiosciences.com)
Strep -tagged proteins are expressed using the pQE TriSystem Vector and obtained at very high purity from cell lysates using Strep -Tactin Superflow Plus affinity purification procedures. (qiagen.com)

Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
The optimal concentration of imidazole needed in the sample and buffer to obtain the best purity and yield differs from protein to protein. (sigmaaldrich.com)
The binding of 12-hr-old and 84-hr-old extracts to CPD- and 6-4PPs under various concentration of NaCl supported the possibility that different damage-recognition protein were expressed in zebrafish according to their developmental condition. (ncl.edu.tw)
We also demonstrated that antibody-capture is effective for purification and concentration of BoNTs from complex matrices such as clinical samples. (cdc.gov)
Endopep-MS currently uses an antibody-affinity concentration/purification step before the enzymatic reaction with the substrate, and the choice of antibody is critical for the success of this assay [ 5 ]. (cdc.gov)

Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
Deep sequencing of RNA molecules cross-linked to immunoprecipitated protein particles (CLIP-seq) revealed. (shengsci.com)
The binding sites for the two quinone molecules are termed Q(P) and Q(D), indicating their positions proximal (Q(P)) or distal (Q(D)) to the site of fumarate reduction in the hydrophilic flavoprotein and iron-sulfur protein subunits. (rcsb.org)
The ABCD4 gene provides instructions for making a protein that is involved in the conversion of vitamin B12 (also known as cobalamin) into one of two molecules, adenosylcobalamin (AdoCbl) or methylcobalamin (MeCbl). (medlineplus.gov)

Next, equilibrate 300 microliters of IgG Sepharose beads with three re-suspensions in purification buffer before adding the beads to the cleared cell lysate for a two-hour incubation with rotating at four degrees Celsius. (jove.com)

We report here that SRE1, a gene encoding a GATA-type protein, bound to promoter sequences of genes involved in siderophore biosynthesis. (nih.gov)
The loss of iron led to a approximately 2.5-fold decrease in DNA-binding affinity, indicating that iron was directly involved in SRE1 regulation of iron-uptake genes. (nih.gov)

These syntaxin 1 protein complexes may play distinct roles in presynaptic biology. (jneurosci.org)
Unlike the embryonic extract, the extracts of 84-h-old larvae produced only low-shifting binding complexes when incubated with a CPD-specific probe. (ncl.edu.tw)

Sterol regulatory element-binding transcription factor 1 (SREBF1) also known as sterol regulatory element-binding protein 1 (SREBP-1) is a protein that in humans is encoded by the SREBF1 gene. (wikipedia.org)
Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as transcription, splicing, and translation, but also in newer fields such as RNA interference and gene regulation by non-coding RNAs. (abcam.com)
A novel cross-phylum family of proteins comprises a KRR1 (YCL059c) gene which is essential for viability of Saccharomyces cerevisiae cells. (nih.gov)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
In the lysosomal membrane, the ABCD4 protein interacts with another protein called LMBD1 (produced from the LMBRD1 gene). (medlineplus.gov)
ABCD4 gene variants involved in this condition lead to production of an abnormal ABCD4 protein that is unable to function. (medlineplus.gov)

A high-performing capture method is a critical bedrock asset for developing industrial purification processes. (bioprocessintl.com)

The small Strep- tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep- Tactin resins. (qiagen.com)

We show also that unstable RAR-RXR heterodimers were formed in solution, and that C-terminal sequences and the DNA-binding domains of both receptors were required for efficient formation of stable heterodimers on response elements. (nih.gov)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
C protein binds strongly to pifO, and weakly to sequences in the ori-1 region. (shengsci.com)
Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. (gbiosciences.com)

The protein was homogeneous by the criteria of gel filtration on Sephadex G-100 and electrophoresis on sodium dodecyl sulphate-polyacrylamide gels, had molecular weight of 36 000 ± 1000 and, unlike the chicken egg white protein (Mr 32 000 ± 2000), was devoid of covalently-bound carbohydrate. (iisc.ac.in)

CLIP is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP) but differs from RIP in the use of UV radiation to cross-link RNA-binding proteins to the RNA that they are bound to. (abcam.com)
2011. "iCLIP -Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution. (abcam.com)
2014. "iCLIP: Protein-RNA interactions at nucleotide resolution. (abcam.com)
The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
A trehalose-functionalized photoaffinity nanoprobe that could photo-crosslink the protein receptor on mycobacteria was developed to investigate these specific interactions further. (uml.edu)

The second part is a pair of regulatable protein expression vectors that permit in vivo production of the target protein at levels appropriate for genetic experiments. (scipeeps.com)
1 The ideal solution would be to create a resin that is completely specific to the target protein, enabling one-step purification. (the-scientist.com)
the target protein binds to the column, and unwanted proteins are eluted. (the-scientist.com)
The binding capacity can vary depending on the size of the target protein, binding conditions and the accessibility of the His-tag. (neb.com)

The iminodiacetate binds divalent cobalt ion with a capacity of 20-40μmoles Co 2+ /ml resin. (gbiosciences.com)

A highly specific system for efficient enzymatic removal of tags from recombinant proteins. (qiagen.com)

Abstract: For the first time, a protein capable of bonding to fatty acids has been purified and described in a parasitic protozoan. (hebron.edu)

Wash with binding buffer until the absorbance reaches the baseline. (sigmaaldrich.com)

Because staphylococcal Protein A (ProtA) binds specifically to IgG, it has been used for many immunological manipulations, most notably antibody purification and diagnostics. (huji.ac.il)
Novus Biologicals™ AbPure Magnetic Antibody Purification Kit is designed to be compatible with Lightning-Link, Thunder-Link PLUS, InnovaCoat GOLD and LATEX conjugation kits. (fishersci.com)
The binding activity expressed in embryos was apparently unrelated to human DNA repair damage-recognition proteins XPA、RPA-70 and RPA-32, since those polypeptides recognized by an anti-human XPA、RPA-70 and RPA-32 antibody were detected only in 84-h-old zebrafish extracts. (ncl.edu.tw)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Strep- tagged proteins bind with high affinity and specificity to Strep- Tactin, an engineered form of streptavidin (see table Protein binding capacities of Strep- Tactin matrices). (qiagen.com)

A competitive binding assay indicated that both CPDs and 6-4PPs were recognized by the same binding activity expressed in 12-h-old zebrafish. (ncl.edu.tw)
In the early 1970s, Belsey et al and Haddad et al developed a competitive binding assay to measure serum vitamin D levels. (medscape.com)

Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast. (jove.com)
Purifying the SNB-BAR proteins from yeast cells allows the acquisition of native homo and hetero SNX-BAR dimers while minimizing the toxicity and insolubility frequently encountered with non-native expression systems. (jove.com)
Our methods can be used to understand the lipid specificities of all yeast SNX-BARs or to produce recombinant SNX-BAR proteins from any other organism. (jove.com)
Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus. (nih.gov)

The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. (cshlpress.com)

Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
1994). High-res- belongs to the family of intracellular lipid bind- olution crystal structures (Sacchetti ni et al. (lu.se)
They form an integral part of the ATP-sensitive potassium channel complex which has two intracellular nucleotide folds that bind to sulfonylureas and their analogs. (bvsalud.org)

The bifunctional chimaeric protein can bind up to 23.4 mg/ml human IgC at a ratio of 1 mol of ProtA-CBD/2 mol of human IgC, and can purify up to 11.6 mg/ml rabbit IgC from a serum. (huji.ac.il)
This assay used vitamin D-binding protein (DBP) obtained from rat serum as the binding agent and exploited the capacity of vitamin D in test samples to displace bound [3 H]25(OH)D3 from DBP. (medscape.com)

Co-crystallization studies of the E. coli QFR with the known quinol-binding site inhibitors 2-heptyl-4-hydroxyquinoline-N-oxide and 2-[1-(p-chlorophenyl)ethyl] 4,6-dinitrophenol establish that both inhibitors block the binding of MQH(2) at the Q(P) site. (rcsb.org)
Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)

Crystal structure of C. elegans SMG8-SMG9 core complex reveals that SMG8-SMG9 heterodimer is formed through the contacts between a C-terminal guanine nucleotide-binding (G) domain of SMG9 and an N-terminal G-like domain of SMG8. (nature.com)

engineering proteins, nucleic acids, or nanomaterials with novel structures, dynamics, and functions for basic biology/biomedical applications and effective therapeutic approaches. (umassmed.edu)

Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)

This enzyme helps break down certain protein building blocks (amino acids), fat building blocks (fatty acids), and cholesterol. (medlineplus.gov)

By periodically monitoring the karyotype of the cells during the scale-up process, it is possible to guarantee the presence of the target marker, assuring that the cell will in fact produce the desired protein. (genengnews.com)
Lower expression of ACBP/DBI protein in KYSE-70 cells is consistent with the predicted expression pattern. (cellsignal.com)

This study introduces pretreatment methods via extruders that disrupt the molecular structure of post-consumer polyester waste, improving binding sites for enzyme reaction. (uml.edu)

Resuspend the pellet in 15 milliliters of purification buffer to achieve a final volume of about 30 milliliters and load the cell suspension onto a homogenizer chilled to four degrees Celsius. (jove.com)
Add 250 μl of Lysis/Binding buffer to the column. (neb.com)
Centrifuge column at 800 x g for 1 minute, discard the Lysis/Binding buffer. (neb.com)
Equilibrate the column with 5 to 10 column volumes of binding buffer at a ﬂow velocity of 150 cm/h. (sigmaaldrich.com)

It was similar to the chicken riboflavin-binding protein in its behavior on ion-exchange celluloses and affinity to interact with the flavin and its coenzymes, but differed significantly in amino acid composition in that it completely lacked proline and contained less of methionine and arginine. (iisc.ac.in)
The body uses methionine to make proteins and other important compounds. (medlineplus.gov)

Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex. (jci.org)

In vivo , BoNT cleaves proteins necessary for nerve signal transmission. (cdc.gov)
This method can detect all seven known BoNT serotypes and involves incubating BoNT with a peptide substrate that mimics each toxin's natural in vivo neuronal protein target. (cdc.gov)

Remove the clarified protein lysate supernatant and transfer to a new microcentrifuge tube on ice, retain a 2 µl aliquot of the clarified lysate for SDS-PAGE analysis. (neb.com)
Add up to 500 μl of the protein sample lysate to the column. (neb.com)

Immobilization is required for most of these applications, Here we describe a genetic-engineering approach to immobilizing ProtA on cellulose, by fusing it to cellulose-binding domain (CBD) derived from the cellulose-binding Protein A of Clostridium cellulovorans. (huji.ac.il)

We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. (wikipedia.org)

The ability to bind functionally active CBD-affinity reagents to cellulosic microtitre plates was demonstrated. (huji.ac.il)

Anatomy15

Organisms11

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment67

Phenomena and Processes71

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science3

Health Care4

Isolation3

Histidine tagged proteins5

Escherichia3

6xHis-tagged proteins1

Prokaryotic and eukaryotic1

Eukaryotic4

Fusion proteins1

Chromatography9

Characterization1

Plasmid5

MRNA6

Synthesis3

Vitro3

Expression and purification3

Assays2

Sequence6

Enzymes4

Metabolism1

Residues2

Elution8

Affinity purification3

Concentration5

Molecules4

Sepharose1

Genes2

Complexes2

Gene6

Processes1

Purity1

Sequences4

20001

Interactions5

Target protein4

Divalent1

Enzymatic1

Abstract1

Wash1

Antibody3

Specificity2

Competitive bindi2

Yeast4

Protocols1

Intracellular3

Serum2

Coli2

Nucleotide1

Nucleic1

Stability1

Amino acids1

Cells2

Enzyme1

Buffer4

Methionine2

Ribonucleoprotein1

Vivo2

Lysate2

Immobilization1

Nuclear2

Functionally1