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  • vectors
  • Virtually all plasmid vectors in common use encode one or more antibiotic resistance genes as a selectable marker, for example a gene encoding ampicillin or kanamycin resistance, which allows bacteria that have been successfully transformed to multiply uninhibited. (wikipedia.org)
  • Vectors are generally derived from plasmids or viruses, and represent relatively small segments of DNA that contain necessary genetic signals for replication, as well as additional elements for convenience in inserting foreign DNA, identifying cells that contain recombinant DNA, and, where appropriate, expressing the foreign DNA. (wikipedia.org)
  • vector
  • Bacteria that have not taken up the plasmid vector are assumed to lack the resistance gene, and thus only colonies representing successful transformations are expected to grow. (wikipedia.org)
  • There are commercially available plasmids that have these features incorporated (such as the pPICZα vector). (wikipedia.org)
  • Once the vector is set up with flanking restriction sites, all manipulations (i.e., mutagenesis, sequencing, expression) can be performed in the same plasmid. (wikipedia.org)
  • Genome
  • Its plasmid collection contains plasmids used for functions such as genome engineering (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity. (wikipedia.org)
  • facilitates
  • Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to not-for-profit laboratories around the world. (wikipedia.org)
  • biomolecules
  • Depending on the column size, CIM monolithic columns are primarily used for the purification or analysis of large biomolecules which are being used for cancer gene therapy, treatment of viral infectious diseases and treatment of genetic diseases. (wikipedia.org)
  • coli
  • E. coli strains are often genetically modified to contain a mutant recA allele and thereby ensure the stability of extrachromosomal segments of DNA, known as plasmids. (wikipedia.org)
  • Plasmid pHT01 is generally stable in both B. subtilis and Escherichia coli, and can be used for protein expression in these host strains. (wikipedia.org)
  • sequence
  • Sequence Analyzer-An Addgene software tool for creating plasmid maps from sequences with annotated features and restriction sites. (wikipedia.org)
  • cell
  • I am purifying plasmid DNA using Promega miniprepa DNA Purification System from Novablue cell transformated by Clontech pEGFP-N1 containing the insert. (bio.net)
  • Miniprep of large number of plasmids can also be done conveniently on filter paper by lysing the cell and eluting the plasmid on to filter paper. (wikipedia.org)
  • Transformants retain the plasmid throughout cell divisions such that it can be recovered and used in other applications. (wikipedia.org)
  • The MS2-GFP fusion protein was expressed by transferring it to a cell with a plasmid (Robert Singer's lab). (wikipedia.org)
  • buffer
  • After washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. (clontech.com)
  • scientists
  • The organization covers the operating costs of maintaining and improving the collection by charging a nominal fee to scientists requesting plasmids. (wikipedia.org)
  • System
  • By using the standard Universal Biological Material Transfer Agreement (UBMTA) and implementing electronic signatures, Addgene's electronic MTA (eMTA) system expedites the approval process for plasmid orders. (wikipedia.org)
  • expression
  • (A) Human ES (H9) cells or Human Episomal iPSCs were transfected with Lipofectamine Stem Reagent or FuGENE HD Regent according to the accompanying protocol and either a 5 kb or 11 kb DNA plasmid expressing GFP and examined for GFP expression 24 hours posttransfection. (thermofisher.com)
  • Once the vector is set up with flanking restriction sites, all manipulations (i.e., mutagenesis, sequencing, expression) can be performed in the same plasmid. (wikipedia.org)