Partial Purification and Characterization of a Heat Stable α-Amylase from a Thermophilic Actinobacteria, Streptomyces sp. (hindawi.com)
Title: Purification and characterization of a novel alginate lyase from a marine streptomyces species isolated from seaweed Abstract: Alginate, a natural polysaccharide derived from brown seaweed, is finding multiple applications in biomedicine via its transformation through chemical, physical, and, increasingly, enzymatic processes. (up.pt)
I. O- Phosphorylethanolamine phospho-lyase: partial purification and characterization. (qmul.ac.uk)
Here, we used Translating Ribosome Affinity Purification (TRAP) combined with RNA-seq to allow the characterization of mRNA and sRNA populations associated to polysomes (referred as the translatome). (conicet.gov.ar)
The characterization of dynamic changes in the translatome of Medicago truncatula roots at early stages of the root nodule symbiosis led us to the identification of mRNAs that significantly increased or decreased their levels of association with polysomes, some of which play essential roles in nodulation (e.g., pectate lyase, SINA and NCR secreted peptides). (conicet.gov.ar)
1. O'Brien, W. E. and Barr, R. H. (1981) 'Argininosuccinate lyase: purification and characterization from human liver', Biochemistry, 20(7), pp. 2056-2060. (bomiprot.org)
These SP constitute an important ingredient of cell walls and get harvested by suitable extraction or precipitation method, followed by purification, characterization and biological studies (Fig. 2 ). (springer.com)
Enzyme6
The enzyme L-serine ammonia-lyase (EC 4.3.1.17) catalyzes the chemical reaction L-serine = pyruvate + NH3 (overall reaction) (1a) L-serine = 2-aminoprop-2-enoate + H2O (1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) (1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. (wikipedia.org)
The systematic name of this enzyme class is L-serine ammonia-lyase (pyruvate-forming). (wikipedia.org)
The optimal pH of this enzyme is 8.5 and the optimal temperature is 45¿C. It is a salt-tolerant alginate lyase with an optimal activity at 0.6 M NaCl. (up.pt)
The main breakdown products of the enzyme on alginate were disaccharides, trisaccharides, and tetrasaccharides, which demonstrated that this enzyme acted as an endo-type alginate lyase. (up.pt)
The enzyme activity of PGLA-rep4, measured at 235 nm with 0.2% apple pectin as the substrate, was 554.0 U/mL, and the specific enzyme activity after purification using a nickel column was 822.9 U/mg. (springeropen.com)
The T. vaginalis and P. putida alpha,gamma-lyases readily separate on a phenyl-Sepharose 6 FF column with the T. vaginalis enzyme eluting first. (unipr.it)
Pectate lyase1
Alkaline pectate lyase has developmental prospects in the textile, pulp, paper, and food industries. (springeropen.com)
Ammonia lyases1
Most of the ammonia lyases have affinity to both phenylalanine and tyrosine, with a strong preference for phenylalanine. (ruc.dk)
Alginate lyase2
In this study a novel alginate lyase, AlyDS44, was purified and characterized from a marine actinobacterium, Streptomyces luridiscabiei, which was isolated from decomposing seaweed. (up.pt)
AlyDS44 is a bifunctional alginate lyase, active on both polyguluronate and polymannuronate, though it preferentially degrades polyguluronate. (up.pt)
Specificity1
These properties of high recombinant expression in E. coli, a simple and effective high-yield purification procedure and high relative specificity for homocysteine with respect to cysteine, make rHCYase a promising candidate to use for the diagnosis of hyperhomocystenemia, which has been demonstrated to be a major risk factor for the onset and mortality of cardiovascular disease of all types. (unipr.it)
Enzymes3
Whereas bacteria such as Escherichia coli have been known for some time to cleave carbon-phosphorus (C-P) bonds in unactivated alkylphosphonates, the enzymes responsible for C-P lyase activity have resisted detection or purification. (embl-heidelberg.de)
As tyrosine can also be a precursor for naringenin production via tyrosine ammonia lyase (TAL), we evaluated in vivo the activity of several TAL enzymes in S. cerevisiae. (ruc.dk)
This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. (antibodies-online.com)
Aspergillus2
PRODUCT DESCRIPTION Phospholipase SBE-02LI is produced by submerged fermentation of Aspergillus niger followed by purification, formulation and drying. (sunsonenzymes.com)
PRODUCT DESCRIPTION Fungal α-amylase is produced by submerged fermentation of Aspergillus Oryzae followed by purification and formulation. (sunsonenzymes.com)
Pectin5
In this study, we selected BacPelA, the pectin lyase with the highest expression activity from Bacillus clausii , modified and expressed in Escherichia coli BL21(DE3). (springeropen.com)
The optimum pH and temperature of the modified pectin lyase (PGLA-rep4) were 11.0 and 70 °C, respectively. (springeropen.com)
After approximately 20 ns of molecular dynamics simulation, the structure of the pectin lyase PGLA-rep4 tended to be stable. (springeropen.com)
Hydrolase breaks the α-1,4 glycosidic bond of the pectin polymer main chain, while lyase breaks the main chain by trans-elimination, creating an unsaturated bond at the non-reducing end (Fu et al. (springeropen.com)
Argininosuccinate lyase (ASL) catalyzes the fourth reaction in this cycle, resulting in the breakdown of argininosuccinic acid to arginine and fumarate. (bomiprot.org)
2.Nagamani SC, Erez A, Lee B. Argininosuccinate lyase deficiency. (bomiprot.org)
Isolation1
A method for the isolation and purification of peptidoglycan heparan sulfate (HS) from bovine liver is described. (rpi.edu)
Polysaccharide1
The highly conserved regions of its amino acid sequences indicated that AlyDS44 belongs to the polysaccharide lyase family 7. (up.pt)
Protein5
PCC 6803 slr 1649-encoded protein also acts as a bilin lyase, and additionally contributes to linker attachment and/or stability of phycobilisomes. (biomedcentral.com)
PCC 6803 and indicate that after separating from a common ancestor protein, the phycoerythrin lyase from Guillardia theta has retained its capacity to couple a bilin group to other phycobiliproteins. (biomedcentral.com)
Recently Zhao and co-workers discovered that a CpeS-like protein functions as a phycocyanobilin-cysteine-beta84 lyase in Anabaena sp. (biomedcentral.com)
The recombinant homocysteine alpha,gamma-lyase (rHCYase) is expressed as the major protein in the host E. coli cells. (unipr.it)
PHENYLALANINE1
In this set up naringenin was produced from phenylalanine by action of a phenylalanine ammonia lyase (PAL) in a strain overproducing phenylalanine and tyrosine. (ruc.dk)
Genes1
Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. (embl-heidelberg.de)
Proteins4
Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins with a specific Grade. (antibodies-online.com)
Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins for a variety of species such as anti-Human N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Xenopus laevis N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Cow N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase). (antibodies-online.com)
Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins validated for a specific application such as ELISA, WB, SDS, AP. (antibodies-online.com)
The SnAvi-tag is a novel in-vivo fluorescent tag combining several features to allow for tandem-purification, western blotting and in-vivo subcellular localization of proteins fused to it. (uni-freiburg.de)
Biol1
The purification of uroporphyrin I and the nature of the Waldenström's uroporphyrin, as isolated from porphyria material (1945) J. Biol. (uba.ar)
Species1
Metabolism of ethanolamine and 1-aminopropan-2-ol in species of Erwinia and the roles of amino alcohol kinase and amino alcohol o- phosphate phospho-lyase in aldehyde formation. (qmul.ac.uk)
Activity4
The Trichomonas rHCYase is selective for homocysteine with respect to very low cysteinase activity in contrast to the alpha,gamma-lyase from Pseudomonas putida, which has very high cysteinase activity with respect to homocysteine. (unipr.it)
Del C. Ríos de Molina, M. Erythrocyte porphyrinogen carboxy-lyase activity in porphyria cutanea tarda and certain other human porphyrias. (uba.ar)
de Viale, L.C.S.M. 'Erythrocyte porphyrinogen carboxy-lyase activity in porphyria cutanea tarda and certain other human porphyrias' (1980) Clinica Chimica Acta. (uba.ar)
Has DNA lyase activity and introduces double-stranded breaks at abasic sites. (thermofisher.com)
Formulation1
PRODUCT DESCRIPTION Hemi-Cellulase SBE-02X is produced by submerged fermentation of Trichoderma reesei followed by purification, formulation and drying. (sunsonenzymes.com)
Detection1
Worldwide delivery on DNA & RNA Purification Kits, qPCR detection kits, mastermixes, polymerases and much more. (nzytech.com)
Coli1
Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. (unipr.it)
Properties1
Purification and Properties of an Alcohol Dehydrogenase Isozyme from a Methanol-using Yeast, Candida sp. (aminer.cn)
Include1
Other names in common use include serine deaminase, L-hydroxyaminoacid dehydratase, L-serine deaminase, L-serine dehydratase, and L-serine hydro-lyase (deaminating). (wikipedia.org)
Plants1
However, industrial production of flavonoids based on purification from plants or on their organic synthesis can be problematic. (ruc.dk)