Loading...
  • quantify
  • A volumetric method to identify, quantify, and localize MAT in rodent bone has been recently developed, requiring osmium staining of bones and μCT imaging, followed by advanced image analysis of osmium-bound lipid volume (in mm3) relative to bone volume. (wikipedia.org)
  • cell
  • The CCD camera operates in TDI (time delay integration) mode that electronically tracks moving objects by moving pixel content from row to row down the rows of pixels in synchrony with the velocity of the object (cell) in flow as measured by the velocity detection system. (emdmillipore.com)
  • TDI imaging combined with spectral decomposition allows the simultaneous acquisition of multiple spectral images (Multispectral Imaging) of each cell in flow. (emdmillipore.com)
  • Quantitation of conjugate formation and representative cells images show that without specific peptide ADAP (green) is distributed around the T cell and in the presence of specific peptide ADAP moves to the synapse between the APC and T cell. (emdmillipore.com)
  • In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus. (wikipedia.org)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • Modern flow cytometers are able to analyze many thousand particles per second, in "real time," and, if configured as cell sorters, can actively separate and isolate particles at similar rates having specified optical properties. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. (wikipedia.org)
  • This has led to the commercial availability of automated image cytometers, ranging from simple cell counters to sophisticated high-content screening systems. (wikipedia.org)
  • Cell sorters are flow cytometers capable of sorting cells according to their characteristics. (wikipedia.org)
  • The historic information available for each cell allow time-lapse cytometers to predict the fate of a cell or to characterize its state without using the phototoxic fluorochromes that are commonly used by flow and image cytometers. (wikipedia.org)
  • This allows a time-lapse cytometer to be placed inside a conventional cell culture incubator to facilitate continuous observation of cellular processes without heat building up inside the incubator. (wikipedia.org)
  • The early history of cytometry is closely associated with the development of the blood cell counting. (wikipedia.org)
  • When no cell is in the gap, electricity flows unabated, but when a cell is sucked into the gap the current is resisted. (wikipedia.org)
  • Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (wikipedia.org)
  • In recent years, acoustic tweezers has found many important applications in the area of manipulating sub-millimeter objects, such as flow cytometry, cell separation, cell trapping, single-cell manipulation, and nanomaterial manipulation. (wikipedia.org)
  • magnetic
  • Magnetic resonance imaging (MRI) provides MAT assessment in the vertebral skeleton in conjunction with μCT-based marrow density measurements. (wikipedia.org)
  • markers
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)