• quantify
  • A volumetric method to identify, quantify, and localize MAT in rodent bone has been recently developed, requiring osmium staining of bones and μCT imaging, followed by advanced image analysis of osmium-bound lipid volume (in mm3) relative to bone volume. (wikipedia.org)
  • cell
  • The CCD camera operates in TDI (time delay integration) mode that electronically tracks moving objects by moving pixel content from row to row down the rows of pixels in synchrony with the velocity of the object (cell) in flow as measured by the velocity detection system. (emdmillipore.com)
  • Mutation frequencies are then determined for sorted mother cells, and the age of the mother cells is determined using flow cytometry by staining with a fluorescent reagent that detects bud scars formed on their cell surfaces during cell division. (jove.com)
  • Now you can image FISH spots or other sub-cellular features no matter where they lie in the cell. (merckmillipore.com)
  • In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus. (wikipedia.org)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (Fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • Modern flow cytometers are able to analyze many thousand particles per second, in "real time," and, if configured as cell sorters, can actively separate and isolate particles at similar rates having specified optical properties. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. (wikipedia.org)
  • This has led to the commercial availability of automated image cytometers, ranging from simple cell counters to sophisticated high-content screening systems. (wikipedia.org)
  • Cell sorters are flow cytometers capable of sorting cells according to their characteristics. (wikipedia.org)
  • This allows a time-lapse cytometer to be placed inside a conventional cell culture incubator to facilitate continuous observation of cellular processes without heat building up inside the incubator. (wikipedia.org)
  • The early history of cytometry is closely associated with the development of the blood cell counting. (wikipedia.org)
  • When no cell is in the gap, electricity flows unabated, but when a cell is sucked into the gap the current is resisted. (wikipedia.org)
  • In recent years, acoustic tweezers has found many important applications in the area of manipulating sub-millimeter objects, such as flow cytometry, cell separation, cell trapping, single-cell manipulation, and nanomaterial manipulation. (wikipedia.org)
  • data
  • b) analyzing the image data collected to detect the disease condition in a subpopulation of the cells. (google.com)
  • 2. The method of claim 1 , further comprising the steps of imaging a diseased population of cells known to exhibit the disease condition to obtain image data indicative of the disease condition, to identify at least one marker indicative of the disease condition. (google.com)
  • 4. The method of claim 3 , wherein the step of analyzing the image data comprises the step of determining if the population of cells includes a larger proportion of lymphocytes than would normally be present in a healthy population of cells. (google.com)
  • d) analyzing the new image data to evaluate an effectiveness of the treatment. (google.com)
  • 6. The method of claim 1 , wherein the step of imaging the population of cells to obtain the image data comprises the step of imaging the population of cells while there is relative movement between the population of cells and an imaging system used to image the population of cells. (google.com)
  • Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing flow cytometry data using extensive computational resources and tools. (wikipedia.org)
  • Open standards, data and software are also key parts of flow cytometry bioinformatics. (wikipedia.org)
  • Flow cytometry data is in the form of a large matrix of intensities over M wavelengths by N events. (wikipedia.org)
  • cells
  • Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. (google.com)
  • Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. (google.com)
  • For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected. (google.com)
  • Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. (wikipedia.org)
  • To detect specific molecules when optically characterized, cells are in most cases stained with the same type of fluorochromes that are used by image cytometers. (wikipedia.org)
  • Conventional flow and image cytometers have the disadvantage of not being able to observe cells over time. (wikipedia.org)
  • The rapid decrease in the cost of digitally storing and processing video information has led to the development of image cytometers which monitor cultured cells using time-lapse video recordings. (wikipedia.org)
  • provides
  • Magnetic resonance imaging (MRI) provides MAT assessment in the vertebral skeleton in conjunction with μCT-based marrow density measurements. (wikipedia.org)
  • offers
  • 2016 Group Exhibition: December 3, 2016 - February 4, 2017 The Image Flow's gallery offers rotating exhibits in different photographic styles for all members of our community to enjoy. (kasi-time.com)
  • device
  • The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster, filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen. (wikipedia.org)