• The initiative is projected to take 10 years (until 2021), and will focus on analysing homozygous mutant mice generated on an isogenic C57BL/6N background by the International Knockout Mouse Consortium. (wikipedia.org)
  • In an analysis of 97 studies, we characterized the ALS progression for the high transgene copy control SOD1 G93A mouse on the basis of disease onset, overall lifespan, and disease duration for male and female mice on the B6SJL and C57BL/6J genetic backgrounds and quantified magnitudes of differences between groups. (nih.gov)
  • C57BL/6 background mice show delayed onset of symptoms, increased lifespan, and an extended disease duration compared to their sex-matched B6SJL counterparts. (nih.gov)
  • Female B6SJL generally experience extended lifespan and delayed onset compared to their male counterparts, while female mice on the C57BL/6 background show delayed onset but no difference in survival compared to their male counterparts. (nih.gov)
  • The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. (mmrrc.org)
  • On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. (mmrrc.org)
  • Furthermore, 129-strain ES cells often contribute to the germline of the chimeras, and when breeding chimeras with C57BL/6 test mice, the dominant agouti color in offspring signifying germline transmission is also easily detected in the G1 generation litters. (umassmed.edu)
  • However, the resulting targeted G1 mice are of a mixed 129 x C57BL/6 genetic background. (umassmed.edu)
  • Depending upon the study, these mice often have to be backcrossed 9 more generations with C57BL/6 mice to place the mutant allele on an inbred C57BL/6 background. (umassmed.edu)
  • Recently, researchers from the Sanger Institute (UK) have isolated stable and germline competent embryonic stem (ES) cells from C57BL/6N mice (1). (umassmed.edu)
  • These ES cells allow for the generation of gene‐targeted mice on a C57BL/6 genetic background without the need for multiple backcrossing of mice. (umassmed.edu)
  • To generate suitable C57BL/6 embryonic stem cells for gene targeting experiments, the Sanger team established a male cell line (JM8) from the N-substrain of C57BL/6 mice. (umassmed.edu)
  • When these C57BL/6N-based JM8 cells were injected into blastocysts from albino mice, a high proportion of chimeras displayed ES cell contribution to both germline and somatic tissues. (umassmed.edu)
  • However, a disadvantage of this combination is that a slightly mixed C57BL/6 genetic background (C57BL/6N x C57BL/6J) is produced when breeding the resulting chimeras with C57BL/6J Tyrc-Brd albino mice to test for germline transmission in the G1 generation. (umassmed.edu)
  • Of course, one could breed the chimeras with inbred C57BL/6N mice to maintain a pure C57BL/6N substrain background- but then one could not use coat color in the G1 generation to identify germline-transmitting chimeras, as all the offspring would be of a black coat color regardless of germline transmission. (umassmed.edu)
  • The non-agouti mutation in C57BL/6 strains is due to an 11.8 Kbp retrotransposon located in the first intron of the agouti gene that abolishes the expression of the Agouti gene. (umassmed.edu)
  • The Sanger group performed gene targeting in the JM8 ES cells to delete the retrotransposon from the agouti locus and restore agouti gene function, permitting the visualization of ES cell-derived mice on an inbred C57BL/6 background by agouti coat color. (umassmed.edu)
  • Since these JM8A3 cells are heterozygous for the corrected agouti allele [A tm1brd ], crossing the resulting brown-on-white chimeras with C57BL/6N test mice yield embryonic stem cell-derived offspring with either agouti or black coats. (umassmed.edu)
  • The UMass Chan Transgenic Animal Modeling Core (TAMC) also has JM8-strain ES cells for targeting, and routinely injects either JM8.F6-strain ES cells or JM8.A3-strain ES cells into albino C57BL/6J Tyrc-Brd blastocysts. (umassmed.edu)
  • The most dramatic observation was that both Dmd(mdx) and Dmd(mdx3cv) mice showed extreme fatigue after testing, while mice from the same C57BL strains appeared unaffected. (ox.ac.uk)
  • Shank1 mutant mice were generated previously and backcrossed with C57BL/6 and 129SvJae strains (gift from M. Sheng and R. Jaenisch Massachusetts Institute of Technology Cambridge MA) (Hung 14 (DIV14) with 4% paraformaldehyde in PBS. (gasyblog.com)
  • The predicted mean onset was 99.86±2.161 days for mixed sex B6SJL groups and 111.4±2.857 days for the mixed C57BL/6 mice (Fig. 2A). (pkc-inhibitor.com)
  • If strain is taken as the independent covariate the main effect estimates onset as 99.27±1.79 days for the DL-Adrenaline B6SJL mice and 109.9 ± 2.063 days for the C57BL/6. (pkc-inhibitor.com)
  • Within the B6SJL group female mice showed later onsets than male mice (= 0.001) and the male C57BL/6 mice showed onsets earlier than both the mixed and female C57BL/6 mice ( (pkc-inhibitor.com)
  • N1 were then mated again to C57BL/6NCrl breeders, to generate N2 mice, identified by PCR and/or sequencing. (mmrrc.org)
  • Among several associations, we focus on a missense mutation in Lipe linked to an increase in yellow fundus spots in C57BL/6J mice. (bvsalud.org)
  • Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. (cdc.gov)
  • It also lead to an exacerbation of disease phenotype in transgenic mice expressing different SOD1 mutants 34 , 35 . (nature.com)
  • transgenic mice. (nih.gov)
  • To visualize the glutamatergic neurons, we used MS-DBB slices from transgenic mice in which the green fluorescent protein is expressed specifically by vesicular glutamate transporter 2-positive neurons and characterized their properties using whole-cell patch-clamp technique. (jneurosci.org)
  • To address these questions, we used transgenic mice in which the expression of the VGLUT2 gene is linked with that of the enhanced green fluorescent protein (eGFP). (jneurosci.org)
  • transgenic mice generated by using BAC clone, cTG: conditional transgenic mice, KO: knockout mice, KI: knock-in mice, cKO: conditional knockout mice, CRIPR/Cas9: Endonuclease-mediated mice. (riken.jp)
  • The International Mouse Phenotyping Consortium (IMPC) is an international scientific endeavour to create and characterize the phenotype of 20,000 knockout mouse strains. (wikipedia.org)
  • IMPReSS, the successor of EMPReSS, is built on the concept of a "phenotype pipeline": a sequence of individual procedures performed on a mouse at a specified age and organized to minimize interference from one procedure to the next. (wikipedia.org)
  • Phenotype data collected from EMPReSS protocols is available at Europhenome. (wikipedia.org)
  • To identify functional categories overrepresented in the differentially expressed genes, we used integrated gene-network analysis, gene ontology enrichment and mouse phenotype ontology analysis. (biomedcentral.com)
  • We generated mice with combined inactivation of the genes coding for SOCS-3 and PTP-1B in brain cells, examined their sensitivity to hormone action, and analyzed the contribution of each gene to the resulting phenotype. (diabetesjournals.org)
  • Surprisingly, the Nestin-Cre mice used to mediate gene inactivation displayed a phenotype. (diabetesjournals.org)
  • Effective high-throughput mouse phenotyping is therefore critically important to establish baseline genotype/phenotype understanding for functional analysis of the KOMP resource. (jax.org)
  • We have also integrated global recommendations of the International Mouse Phenotyping Consortium (IMPC) , which identified important parameters that will collectively summarize the consequences of removing the function of a single gene in the context of the whole animal. (jax.org)
  • These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. (mmrrc.org)
  • A minimum of four (4) mutant mice, heterozygous for the selected mutation on a mixed 129S5;B6 background. (taconic.com)
  • N2 heterozygous mutant mice were mated for production of phenotyping cohorts, and N2 or N2F1 mice were used for cryopreservation purposes. (mmrrc.org)
  • The majority of the homozygous mutant mice exhibited retinal depigmentation spots in the fundus test. (mmrrc.org)
  • RT-PCR analysis revealed that the transcript was absent in the homozygous mutant mice. (mmrrc.org)
  • Homozygous mice are phenotypically normal. (pkd-rrc.org)
  • Homozygous mice develop congenital hepatic fibrosis. (pkd-rrc.org)
  • The standardized phenotyping protocols used to characterize mutant strains are coordinated and presented on The International Mouse Phenotyping Resource of Standardised Screens (IMPReSS) . (mousephenotype.org)
  • To address the question of specificity for the allele encoding the Na V 1.4-R669H variant as a cause of HypoPP and to produce a model system in which to characterize functional defects of the mutant channel and susceptibility to paralysis, we generated knockin mice carrying the ortholog of the gene encoding the Na V 1.4-R669H variant (referred to herein as R669H mice). (jci.org)
  • INFRAFRONTIER will also provide open access to all newly developed disease models through the European Mouse Mutant Archive (EMMA). (infrafrontier.eu)
  • INFRAFRONTIER ® and European Mouse Mutant Archive - EMMA ® are registered trademarks at the European Union Intellectual Property Office (EUIPO). (infrafrontier.eu)
  • Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications. (jove.com)
  • Data parameters are associated with biomedical ontology terms in order to facilitate data sharing and to aid in the identification of phenotypic mouse-models of human diseases. (wikipedia.org)
  • Level I phenotypic analysis is performed on mice from this generation. (mmrrc.org)
  • Testing of SHIRPA, a mouse phenotypic assessment protocol, on Dmd(mdx) and Dmd(mdx3cv) dystrophin-deficient mice. (ox.ac.uk)
  • Broad phenotypic characterization of emerging mouse models will add important value and greatly enhance their utility to the scientific community. (jax.org)
  • However, one still has to genotype all of the G1 generation mice from germline transmitting chimeras or risk losing some black colored mice that are ES cells derived. (umassmed.edu)
  • IMPReSS was launched in 2011 to help the IMPC achieve its goal of characterizing a knockout mouse strain for every gene and will continue to be actively developed for the ten year life-time of the project. (wikipedia.org)
  • Around 30% of all targeted gene knockouts in mice result in embryonic or perinatal death. (wikipedia.org)
  • Cerebellar and hippocampal tissue samples from three En2 -/- and wild type (WT) littermate mice were assessed for differential gene expression using microarray hybridization followed by RankProd analysis. (biomedcentral.com)
  • Samples were hybridized with GeneChip Mouse Gene 1.0 ST (Affymetrix) and scanned at the UCLA Microarray Core Facility. (nih.gov)
  • The targeted mouse gene is Pdgfc (platelet-derived growth factor, C polypeptide), the ortholog of human PDGFC. (mmrrc.org)
  • Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. (mmrrc.org)
  • In an in vitro mammalian cell gene mutation test performed similarly to OECD guideline 476, L5178Y/ TK+/- mouse lymphoma cells were exposed to sodium arsenate at concentrations of 26, 34, 46, 61 and 81 μg/mL in the absence of metabolic activation and the exposure duration was 3 hours. (europa.eu)
  • An in vitro gene mutation assay was conducted with sodium arsenite in the supF gene of pZ189 shuttle vector system in DNA repair proficient GM0637 human fibroblasts to determine whether arsenic alone induces mutation and whether the combination of arsenic and UV irradiation leads to a yield of mutants greater than the sum of the arsenic or UV treatments alone. (europa.eu)
  • Sequence analysis of genomic region or mutated gene: Sequencing of transgenes, targeted genes or any other mutated genes of TG, BAC-TG, cTG, KO, KI, CRISPR/Cas9, cKO and spontaneous/ENU-induced mutant strains. (riken.jp)
  • Exon 1 ENSMUSE00000468303 and flanking splicing regions were constitutively deleted from the Bloc1s4 gene ENSMUSG00000060708 using CRISPR Cas9 gene editing technology in mouse zygotes. (mmrrc.org)
  • Those studies used mice lacking serotonergic neurons resulting from the loss of function mutations in the gene encoding the LIM homeobox transcription factor 1 ( Lmx1b ). (aspetjournals.org)
  • More importantly, we used mice deficient in the gene encoding tryptophan hydroxylase 2 ( Tph2 ), which could not synthesize 5-HT in the brain. (aspetjournals.org)
  • The extensive library of single-gene knockout strains generated through the KOMP program will become significantly more valuable with comprehensive assessments of the functional consequences of removing one gene. (jax.org)
  • The laboratory has focused on optimizing the conditions for viral transduction, including selecting and modifying viral strains for study in animal models, optimizing growth conditions for transduced hematopoietic cells, and designing gene vectors to ensure appropriate placement and expression of the inserted gene. (nih.gov)
  • They routinely provide mouse and rat model development services using all standard approaches such as gene targeting, the generation of chimeric mice from gene-targeted ES cells and gene editing technologies such as TALENs or CRISPR/Cas9. (infrafrontier.eu)
  • Gene mutation (Bacterial reverse mutation assay / Ames test): negative with and without metabolic activation in Salmonella typhimurium strains TA 98, TA 100, TA 1535 and TA 1537 (OECD TG 471) (Hüls AG, 1998a). (europa.eu)
  • Thus, the DHFR DD-based approach allows for control of protein abundance in a variety of mouse models, laying the foundation to use this strategy for the conditional control of gene therapies to potentially treat multiple eye diseases. (bvsalud.org)
  • The mouse strains are characterized in a broad based phenotyping pipeline that is focused on revealing insights into human disease by measuring embryonic, neuromuscular, sensory, cardiovascular, metabolic, respiratory, haematological, and neurological parameters. (wikipedia.org)
  • In 2013 the IMPC published the Bloomsbury report on mouse embryo phenotyping, outlining a standard pipeline for the screening of embryonic-lethal knockouts in homozygote mutants. (wikipedia.org)
  • Aggregation of mutant superoxide dismutase 1 (SOD1) is a pathological hallmark of a subset of familial ALS patients. (nature.com)
  • We have established clear experimental protocols and provide specific guidelines for working, with conformational/misfolded SOD1-specific antibodies. (nature.com)
  • Interestingly, it has been also reported that wtSOD1 can acquire an aberrant conformation, implying a possible shared pathological pathway between mutant SOD1 -linked FALS and SALS 27 . (nature.com)
  • The SOD1 G93A mouse model of amyotrophic lateral sclerosis (ALS) is the most frequently used model to examine ALS pathophysiology. (nih.gov)
  • There is a lack of homogeneity in usage of the SOD1 G93A mouse, including differences in genetic background and gender, which could confound the field's results. (nih.gov)
  • The often-reported increase in lifespan for female mice was observed only for mice on the B6SJL background, implicating a strain-dependent effect of sex on disease progression that manifests despite identical mutant SOD1 expression. (nih.gov)
  • We demonstrate that cDC2 are also expanded in mice carrying the orthologous PTPN22 619W mutation. (frontiersin.org)
  • Figure 1 Homology in domain structures and amino acid sequences between human and mouse NLRP1 and the present knock-in mutation. (frontiersin.org)
  • The previously reported pathogenic mutation in NLRP1 in humans, and the missense mutation we inserted into the BALB/c mice in this study. (frontiersin.org)
  • The protocols used to assess these phenotypes have been standardized across the IMPC partners and are available at IMPReSS. (wikipedia.org)
  • Mouse strains generated by the IMPC partners are deposited at the KOMP repository and the European Mutant Mouse Archive. (wikipedia.org)
  • In many cases, strains carrying one of two types of alleles will be archived - a null allele used in the primary IMPC phenotyping pipeline and a conditional ready allele that allows tissue restricted knockouts via the Cre-Lox Recombination and FLP-FRT recombination systems. (wikipedia.org)
  • Both mouse clinics are engaged in large scale mouse production projects like the IMPC and have a capacity of 100 to 150 model development projects per year. (infrafrontier.eu)
  • The effects of these mutations cannot therefore be studied in live adult mice, except as heterozygote mutants. (wikipedia.org)
  • This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. (biomedcentral.com)
  • A license from third party patent owner(s) may be required for for-profit entities to use mouse models derived from CRISPR-Cas9 technologies and it is the Users sole responsibility to determine whether such a license is necessary. (mmrrc.org)
  • Lipe-/- mice generated using CRISPR-Cas9 technology are found to develop accumulation of subretinal microglia, a retinal degeneration with decreased visual function, and an abnormal retinal lipid profile. (bvsalud.org)
  • The European Mouse Phenotyping Resource for Standardized Screens (EMPReSS), the predecessor for IMPReSS, developed more than a 150 standardized protocols for the characterization of mutant mouse strains across European research institutes as part of the EUMODIC and EUMORPHIA projects. (wikipedia.org)
  • Slc17a7: PV+ neurons 0.19 ± 0.01 excitatory neurons 0.78 ± 0.06 N = 4 mice. (gasyblog.com)
  • We report here that the Fgf2 null mutant mice show an ∼40% decrease in cortical glutamatergic pyramidal neurons. (jneurosci.org)
  • Because Fgf2 −/− mice lacked 45% of cortical neurons at birth, the progenitor loss is compensated only in part during neurogenesis. (jneurosci.org)
  • Because the dorsal PVE was depleted of progenitors but the ganglionic eminences were not affected in Fgf2 −/− mice, we predicted that these mice should lack a population of pyramidal neurons, but their cortical GABA interneurons should have remained unchanged. (jneurosci.org)
  • This method can be applied to testing other pathogens and their mutants, as well as virulence infect in mice. (jove.com)
  • The SHIRPA protocol was proposed as a rapid, comprehensive screening method for qualitatively abnormal phenotypes in the mouse (Rogers et al. (ox.ac.uk)
  • In mice, cDCs (CD11c + MHCII + ) are sub-divided into functionally distinct phenotypes defined as cDC1 (CD8 + IRF8 + XCR1 + Clec9a + CD24 + ) and cDC2 (IRF4 + CD11b + SIRPα + ) whilst in humans the equivalent DC subsets are defined by expression of CD8 + IRF8 + XCR1 + Clec9a + CD141 + (cDC1) and IRF4 + CD1c + (cDC2) ( 1 - 4 ). (frontiersin.org)
  • Perform quantitative and qualitative assessment of phenotypes for each mutant line using automated calling procedures and technical, statistical and scientific review. (jax.org)
  • The pipeline also includes additional disease-relevant phenotypes based on proven protocols and expertise from additional internal and external investigators that comprise our panel of JAX KOMP2 Domain Experts. (jax.org)
  • All work involving infectious H5N1 influenza was performed in government-approved biosafety level 3-enhanced containment facilities with experimental protocols in compliance with applicable federal statutes and institutional guidelines. (cdc.gov)
  • Finally, different experimental protocols (tremor, rotarod, etc.) for onset determination result in notably different onset means. (nih.gov)
  • Join our discussion on the use of reagents and experimental protocols. (pkd-rrc.org)
  • The immunosensor was successfully applied in the detection and quantification of PB1-F2 in infected mouse lungs and cell lines, providing temporal expression profiles of PB1-F2 during viral infection. (omicsonline.org)
  • For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. (taconic.com)
  • After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. (biomedcentral.com)
  • These data suggest that the SHIRPA primary screen is effective not only in identifying subtle neuromuscular mutants, but also in distinguishing qualitative differences between mutants with neuromuscular abnormalities. (ox.ac.uk)
  • Onset category Differences in reported mean onsets between articles that defined their onset determination criteria were evaluated through regression with genetic background strain and the onset category included as categorical predictors (Fig. 3). (pkc-inhibitor.com)
  • (C) Differences between the three mouse NLRP1 paralogs. (frontiersin.org)
  • Our computational biology work investigates the precise makeup of both mouse and human genomes and the similarities (and important differences) important for translating preclinical research to clinical benefit. (jax.org)
  • In addition, these ES cells are the foundation for two large-scale knockout mouse programs designed to provide targeted BL/6 ES cells to the scientific community (EUCOMM and KOMP). (umassmed.edu)
  • The International Mouse Phenotyping Resource of Standardised Screens (IMPReSS) coordinates and presents standardized protocols that are used by mouse research clinics to assess biological characteristics of mutant mouse strains. (wikipedia.org)
  • We would like to ask all the users to confirm the quality and characteristics of our mouse resources as soon as possible, before starting your full scale experiments. (riken.jp)
  • Data was stratified by both the sex … Fig. 2 The effect of G93A mouse genetic background strain and sex on the age at disease onset. (pkc-inhibitor.com)
  • Fig. 3 The variation in onset times by onset determination criteria and genetic background strain. (pkc-inhibitor.com)
  • UV irradiation (320 J/m2) increased the yield of mutants 3.5-fold above the background rate. (europa.eu)
  • All key studies were conducted according to appropriate OECD guidelines, or protocols similar to appropriate guidelines, and in compliance with GLP. (europa.eu)
  • The study of the differential expression profile in bone tissues between Tnni2del175k mutant mice and their wild-type littermates using mRNA array. (nih.gov)
  • No significant increases in mutant frequency were observed following treatment of sodium arsenate in the absence of metabolic activation. (europa.eu)
  • Under the test conditions, sodium arsenate is not considered as mutagenic in mouse lymphoma L5178Y cells. (europa.eu)
  • Poisson distribution followed by student's t-test was used to assess the effects of sodium arsenite on mutant frequencies. (europa.eu)
  • Sodium arsenite alone produced significant increases in the pZ189 mutant frequency at the concentration of 5.0 µM, whereas 1 and 2.5 µM arsenite were not mutagenic. (europa.eu)
  • To test whether this approach could be effectively applied to a wide variety of aged and disease-related ocular mouse models, we evaluated the DHFR DD system in the eyes of aged mice (up to 24 months), a light-induced retinal degeneration (LIRD) model, and two genetic models of retinal degeneration (rd2 and Abca4 -/- mice). (bvsalud.org)
  • This protocol is significant for creating and determining mind-body desired mutations in the genome of Pseudomonas aeruginosa, as well as the testing the effect of mutations on virulence reduction in a reproducible mouse model. (jove.com)
  • Adult females of all genotypes were blood-fed on a single human subject during mutant generation, for behavioral assays, and calcium imaging. (elifesciences.org)
  • To unravel recurrent ASD-related neuropathological mechanisms, we took advantage of the En2 -/- mouse model and performed transcriptome profiling on cerebellar and hippocampal adult tissues. (biomedcentral.com)
  • We generated Nlrp1b (the mouse homologue of human NLRP1 ) gain-of-function knock-in ( Nlrp1b KI) mice with UVB irradiation-induced autoinflammatory skin lesions. (frontiersin.org)
  • We demonstrated that UVB irradiation induces IL-1β upregulation and IL-1β-dependent inflammation via caspase-1 activation in these Nlrp1b KI mice. (frontiersin.org)
  • RNA sequencing revealed the upregulation of inflammasome pathway-related genes, keratinocyte stress marker genes, and keratinocyte differentiation marker genes in the Nlrp1b KI mice after UVB irradiation. (frontiersin.org)
  • The skin inflammation and hyperkeratosis from UVB irradiation in the Nlrp1b KI mice were inhibited by both intraperitoneal and subcutaneous administration of anti-IL-1β antibodies before UVB irradiation. (frontiersin.org)
  • Genetic manipulation of the mouse genome has been customarily performed using ES cells derived from the agouti 129-inbred strain of mouse. (umassmed.edu)
  • Recent progress in genome-editing technology will be used to develop many new rat mutants - in order to model human diseases which cannot be treated effectively today. (infrafrontier.eu)
  • The mixed sex mice were the only sex category to significantly differ on the basis of the genetic DL-Adrenaline background (= 0.002). (pkc-inhibitor.com)
  • A) Linear regression was performed with the mean age at onset for each group modeled as the response with mouse sex DL-Adrenaline and genetic background factored as categorical … When the onset data are modeled as a Coxproportional hazards the predicted survival curve for disease onset shows a difference ( (pkc-inhibitor.com)
  • PCR tests for the genetic background of inbred and wild-derived strains by using microsatellite or single nucleotide polymorphism (SNP) markers. (riken.jp)
  • This knock-in mouse model has a triple HA tag at the C-terminal tail of PKHD1 and produces a fully functional fibrocystin protein. (pkd-rrc.org)
  • In contrast, no change in pyramidal or granule cell number is detected in the hippocampus of Fgf2 −/− mice. (jneurosci.org)
  • It's the first time that the European research infrastructure for modeling human diseases via mammalian genomes expands its unique services to generate not only mouse, but rat models for its users. (infrafrontier.eu)
  • We tested two dystrophin-deficient mutants Dmd(mdx) and Dmd(mdx3cv), both of which are indistinguishable from wild-type by a simple visual assessment, at different ages, using the primary screen of the SHIRPA protocol. (ox.ac.uk)
  • It takes more than two years for the exponential age-related increase in morbidity and mortality to become obvious in well-cared-for, wild-type laboratory mice and rats of a healthy strain. (sens.org)
  • Each strain of dystrophin-deficient mice showed a different profile in locomotor activity and deficiencies in the wire maneuver, righting reflex, and negative geotaxis tests. (ox.ac.uk)
  • Electroencephalographic (EEG) recording also showed decreased sleep in Tph2 -deficient mice. (aspetjournals.org)
  • Fundus spots in mice often correlate with the accumulation of activated subretinal microglia. (bvsalud.org)
  • The DHFR DD was effectively degraded in all model systems, including rd2 mice, which showed significant defects in chymotrypsin proteasomal activity. (bvsalud.org)
  • The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. (mmrrc.org)
  • Genetic QC tests are performed at deposition or before distribution, and the test results and/or protocols are posted in the web catalog. (riken.jp)
  • This mouse model has a deletion of PKHD1 exons 3 through 4. (pkd-rrc.org)
  • But you can easily identify the germline transmitting chimeras by the presence of agouti coat colors in the G1 generation, and performing tail biopsies and genotyping any offspring from that proven chimera will subsequently identify germ-line transmitted mice. (umassmed.edu)
  • In a new Trans-national Access Call INFRAFRONTIER offers open access to its infrastructure and expertise for researchers who need rat mutant models to be generated for their scientific aims. (infrafrontier.eu)
  • Moreover, trimethoprim (TMP) administration stabilized the DHFR DD in all mouse models. (bvsalud.org)
  • Moreover, myeloperoxidase (MPO), an abundant enzyme of inflammatory cells (neutrophils), - involved in the principal defense mechanisms of innate immunity -was also effective in oxidative biodegradation of CNT in biochemical models and in cells yielding the products that did not cause pulmonary inflammation in mice [14] . (cdc.gov)
  • Despite the limited number of animals used in the study, our bioinformatic analysis indicates the En2 -/- mouse is a valuable tool for investigating molecular alterations related to ASD. (biomedcentral.com)
  • Animal experimentation: For routine strain maintenance, animals were blood-fed on live mice or human subjects. (elifesciences.org)
  • Materials and Methods Animals All animal protocols were authorized by the Institutional Animal Care and Use Committee (IACUC) of the University or college of Massachusetts Medical School. (gasyblog.com)
  • The detection and evaluation of concentration of influenza virus proteins in biological samples is critical in a broad range of medical and biological investigations regarding the concern over potential outbreaks of virulent influenza strains in animals and humans. (omicsonline.org)
  • BAL cytology indicated a robust and early (day 1) accumulation of neutrophils - slightly weaker in MPO k/o mice vs w/t animals ( Fig. 1d ), followed by a sequential appearance of macrophages in both groups of animals (with a peak at day 7) (data not shown) [4] . (cdc.gov)
  • The key advantage of this technique is a chrome validation and a reproducibility of mouse infection model. (jove.com)
  • To slow down this process, we use a frozen stops, bag them, hang them on multiple rungs on modification before testing them in the mouse model. (jove.com)
  • This mouse model lacks PKHD1 exon 67. (pkd-rrc.org)
  • Brain-specific inactivation of these genes individually in the mouse partially protects against diet-induced obesity (DIO) and insulin resistance. (diabetesjournals.org)