Protocols culture techniques. Medical search. Frequent questions

It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). (cshlpress.com)

This protocol includes example calculations and can be modified easily to plate cells in different volumes and concentrations. (ohsu.edu)
Described in this protocol is the lipofectamine transfection of plated cells with cDNA and/or siRNA. (ohsu.edu)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
The choice of method depends principally on the nature of the cells themselves, but also on the goals and purpose of the 3D culture. (sigmaaldrich.com)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. (sigmaaldrich.com)
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. (sigmaaldrich.com)
In scaffold-based culture, cells are supported in all dimensions either by an artificial structure or by a polymer network known as a hydrogel. (sigmaaldrich.com)
In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. (wikipedia.org)
Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. (wikipedia.org)
When microorganisms from this culture are transferred into fresh media, nutrients trigger the growth of the microorganisms which will go through lag phase, a period of slow growth and adaptation to the new environment, followed by log phase, a period where the cells grow exponentially. (wikipedia.org)
Subculture is therefore used to produce a new culture with a lower density of cells than the originating culture, fresh nutrients and no toxic metabolites allowing continued growth of the cells without risk of cell death. (wikipedia.org)
It is often important to record the approximate number of divisions cells have had in culture by recording the number of passages or subcultures. (wikipedia.org)
In the case of plant tissue cells, somaclonal variation may arise over long periods in culture. (wikipedia.org)
The protocol for subculturing cells depends heavily on the properties of the cells involved. (wikipedia.org)
Cell density in these cultures is normally measured in cells per milliliter for large eukaryotic cells, or as optical density for 600nm light for smaller cells like bacteria. (wikipedia.org)
Adherent cells, for example many mammalian cell lines, grow attached to a surface such as the bottom of a cell culture flask or petri dish. (wikipedia.org)
In order to reduce the costs associated with the maintenance regime of cultures, efforts were initiated on the establishment of a cryopreserved biobank for long term maintenance of cultures, thus minimizing the efforts associated with handling of material, as successfully frozen cultures, in theory, could be maintained effectively indefinitely, with the advantage of the stability of cells characteristics. (scielo.br)
Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
The culture medium from CD4 + T cell differentiation procedures should be analyzed to confirm that the cells are secreting cytokines relevant to the desired cell subset. (rndsystems.com)
Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)
By using a selective disaggregation technique after intact spheroids had been exposed to the drug it was possible to show directly that the inner spheroid cells were most resistant (D0 = 0.25 μ/106 cells). (researchgate.net)
Culture cells in standard serum-containing or serum-free medium appropriate for the cell type. (sigmaaldrich.com)
Moreover, it has recently been shown that mouse and human ES cells can develop into a three-dimentional (3D) optic cup in culture that remarkably resembles the embryonic vertebrate eye 20 , 21 . (nature.com)
Alvetex 3D cell culture enables cells maintain their in vivo morphology, behavior and responsiveness within an in vitro model system. (reprocell.com)
In conventional 2D cell culture, cells come into contact with the flat surface of the culture vessel (e.g. (reprocell.com)
This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. (jove.com)
This protocol allows us to monitor endogenous mitophagy complexes in human tissues such as beta cells, facilitating mitophagy research in pivotal translationally-relevant tissues. (jove.com)
Contributors present electrophysiological methods to examine single-channel activity in cultured cells, to study synaptic plasticity and circuit dynamics in brain slice preparations, and to perform whole-cell recordings in awake and freely moving animals. (cshlpress.com)
V6.5 ES cells were differentiated into neural progenitor cells (NPCs) using established techniques (Meissner et al. (nih.gov)
1996). Briefly, after expansion, ES cells were trypsinized and cultured in bacterial dishes for 4 days in the absence of LIF to promote embryoid body formation. (nih.gov)
After 5-7 days selection in ITSFn, adherent cells were trypsinized, triturated to a single cell suspension, and re-plated on tissue culture dishes coated with 15 ug/ml poly-L-ornithine (Sigma) and 1 ug/ml human plasma fibronectin (Gibco). (nih.gov)
Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
Proving the presence of GPER in uterine non-pregnant and pregnant smooth muscle culture cells, by PCR techniques. (umfcv.ro)
Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)

After isolation according to standard protocols, culture four to six times 10 to the third human islet equivalents in 10 milliliters of complete pancreatic islet medium for at least one day at 37 degrees Celsius. (jove.com)

A large collection of established methods and protocols in biochemistry, biotechnology, biomedicine and life sciences. (lu.se)

Embryoid bodies were then plated on tissue-culture plastic dishes and cultured in serum-free, defined media (ITSFn). (nih.gov)

A new technique for the assay of infectivity of human adenovirus 5 DNA. (sigmaaldrich.com)

By coupling sequencing and pairwise competition experiments, we demonstrated that the first mutation λ evolved en route to the innovation would only evolve in the presence of the ancestral host, whereas later steps in λ's evolution required the shift to a resistant host. (elifesciences.org)
The Alvetex Scaffold 12 Well Plate is primarily suitable for short term culture experiments where the medium is replaced every 1-2 days. (reprocell.com)

Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
In vitro tissue culture and in vivo animal models. (environmentalchemistry.com)
Proficient on HPLC, in vitro tissue culture and in vivo animal tumor models, and WinNonlin - Industry-Standard PK/PD Modeling and Analysis. (environmentalchemistry.com)
Solicited models include patient-derived cell lines, patient-derived xenograft (PDX) mouse models, and 3D human tissue model culture systems established from racially/ethnically diverse patient populations. (sbir.gov)
3D human tissue model culture systems: While immortalized cell lines have been standard practice in cancer research for decades, adequate modeling of the heterogeneity of human cancer is an unmet need. (sbir.gov)
For example, in one study, culture of samples obtained via sonication of prostheses was more sensitive than conventional periprosthetic-tissue culture for microbiologic diagnosis of prosthetic hip and knee infection, especially in patients who had received antimicrobial therapy within the 14 days preceding surgery. (medscape.com)
From these isolates, 23 strains from scab material and previously lyophilized samples were viable in tissue-culture. (who.int)

In addition, the culture was immunofluorescently labeled with Cy2 conjugated to antibodies that target peroxisomal membrane protein 70 ( PMP 70 ), an abundant and integral membrane component of peroxisomes. (fsu.edu)

We are happy to work together with fellow researchers to troubleshoot or discuss ways to improve techniques. (ohsu.edu)
Furthermore, it has also been responsible for the specialized training of personnel, including researchers which went to initiate other algae culture collections in Brazil. (scielo.br)
In the culture vessel comparison, the researchers used a "traditional" large-capacity (6,320 cm 2 surface area)10-layer vessel and Corning HYPER technology allowing for a more compact (1,720 cm 2 ) 10-layer vessel. (corning.com)
The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects). (benthamscience.com)
In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
This notion is supported by the growing evidence from researchers such as Aida Walqui and colleagues and their work with English learners at WestEd (2019), and Ron Ritchhart and his work around the cultures of thinking out of Harvard's Project Zero (2015). (ascd.org)

The technique of in vitro propagation of Arbuscular mycorrhizal fungi has been developed over the past few decades and opens up areas of studying plant-fungi interactions. (nepjol.info)
The objective of this paper is to find out the in vitro culture of Arbuscular Mycorrhizal Fungi using Root Organ Culture technique. (nepjol.info)
Ascertain of root colonization of these fungi could be affected in vitro without undertaking complex and complicated culture conditions. (nepjol.info)
Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. (stemcell.com)
Managed several discovery oncology projects, supervised several associates, and characterized with comprehensive in vitro and in vivo techniques numerous novel compounds for a variety of chemotherapeutic targets. (environmentalchemistry.com)

Adherent cell culturing is a space intensive task, running the risk of scores of traditional flasks sprawling across lab benches or filling incubators to reach required surface areas. (corning.com)
The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)

A project to develop guidelines to assess ventilation effectiveness in underground coal mines using improved sampling protocols and defining optimal instrument locations for continuous-reading airflow and methane monitors. (cdc.gov)
Having interpreted the above, the clinician, in cooperation with the patient, can better select the appropriate veneering technique, along with the optimal substrate for the veneers. (medscape.com)

Array comparative genomic hybridization (CGH) was performed following the standard protocol provided by Agilent Technologies (Agilent Technologies, Santa Clara, CA, United States). (hindawi.com)

I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
is a compilation of several examples of molecular biology protocols. (benthamscience.com)

For protocols to stain membrane-associated molecules or intracellular molecules (using either detergents or alcohol to permeabilize the cell membrane), please visit Flow Cytometry Protocols on our website. (rndsystems.com)

The use of optogenetic tools to study cellular and circuit dynamics in organotypic slice cultures is also covered. (cshlpress.com)

https://open.oregonstate.education/generalmicrobiology/chapter/microbial-growth/ "Bacterial Culture Techniques. (wikipedia.org)

High signal levels from all three of the fluorophores employed to stain the culture are present. (fsu.edu)

If the appropriate safety equipment or protocols are unavailable, consider referring specimens to an equipped reference laboratory that meets the recommendation above. (cdc.gov)
Because postexposure use of zidovudine is a consideration that requires prompt action, employees who handle blood or any specimens requiring universal precautions should be familiar with this protocol. (cdc.gov)

Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. (cshlpress.com)

Current Protocols in Essential Laboratory Techniques is covering good laboratory practices and resources to be used in the undergraduate laboratory to equip the novice researcher with a solid life science skill set. (lu.se)

After successful amplification and detection, the technique expanded to examine real field soil samples from two sugar beet fields near Sidney, MT area and a sugar beet field at Foxholm, ND. (usda.gov)

Includes step-by-step techniques, procedures, and practical overviews. (lu.se)

When biologists from the Cer Groupe immunobiology laboratory in Belgium wanted to improve viral production yields for use in diagnostic kits, they ran a performance comparison for stacked (multi-layer) culture vessels. (corning.com)
There is a need for both improved diagnostic methods and more efficient treatment protocols. (medscape.com)
Foolproof intraoperative diagnostic techniques, improved implant designs, and better local antibiotic delivery systems must be developed to face the menace of infection associated with joint replacement surgery. (medscape.com)
Currently used diagnostic tests rely on culture and serology but with some limitations. (cdc.gov)

Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)
Spheroid culture can be achieved in diverse environments including low-attachment microplates, bioreactors, and microfluidics culture systems. (sigmaaldrich.com)
Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)

In addition to adding more robust discussion practices, we also want to make these practices more culturally responsive by centering them around the principles of youth culture and collectivist practice. (ascd.org)

Hydrogela are the most widely used systems for 3D cell culture. (sigmaaldrich.com)
Protocols are now widely used in adult learning communities and a growing number of classrooms across the country. (ascd.org)

Compatible with the majority of down stream analytical techniques. (reprocell.com)

This could form an economically viable technique for root organ culture of Arbuscular mycorrhizal fungi. (nepjol.info)

Performs other molecular, cell biological, or biochemical techniques related to these specific lines of research. (umd.edu)

For microorganisms there is a tendency to adapt to culture conditions, which is rarely precisely like the microorganism's natural environment, which can alter their biology. (wikipedia.org)
Traditionally, strains at CCMA-UFSCar were maintained in replicate as metabolically active cultures under suboptimal growth conditions, which must be sub-cultured every 2-3 months (Vieira, pers. (scielo.br)

Cell lines and microorganisms cannot be held in culture indefinitely due to the gradual rise in metabolites which may be toxic, the depletion of nutrients present in the culture medium, and an increase in cell count or population size due to growth. (wikipedia.org)

All sequences were compared by alignment with the pure culture and a C. beticola actin gene sequence from GenBank. (usda.gov)
All sequences as well as C. beticola from amended field soil and pure culture were compared by alignment with a C. beticola actin gene sequence from GenBank. (usda.gov)

This protocol outlines an evaluation of a large-scale care home improvement programme in the West Midlands. (biomedcentral.com)

Multiplex detection techniques enable efficient screening for many cytokines simultaneously. (rndsystems.com)
This technique will enable rapid screening for potential C. beticola problems prior to planting sugar beet, or other host crops and provide information on the effect of soil applied biological control agents and other management techniques on C. beticola and potential inoculum build up or control in field soils. (usda.gov)
This system will enable rapid screening for inoculum potential of C. beticola in soil and determine the effect of soil applied biocontrol agents and other management techniques on C. beticola and inoculum potential in field soils. (usda.gov)

We are committed to ensuring the reproducibility of our findings by electing to share our protocols and present them in a clear and accessible way. (ohsu.edu)
Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (sigmaaldrich.com)

Typically, subculture is from a culture of a certain volume into fresh growth medium of equal volume, this allows long-term maintenance of the cell line. (wikipedia.org)
These cell types can be subcultured by simply taking a small volume of the parent culture and diluting it in fresh growth medium. (wikipedia.org)
For example, the Corning ® HYPERFlask ® cell culture vessels are designed to maximize growth capacity with a minimal footprint. (corning.com)
By the end of the studied period (14th day), the enzymatic method was more efficient in promoting culture growth. (bvsalud.org)

Cell culturing is integral to diverse research fields including cell therapy, gene therapy, diagnostics, and vaccine development, among others. (corning.com)
Beyond the retail aspects of more integral versions working and better ebook Bombs, IEDs, and Explosives : Identification, Investigation, and Disposal Techniques of field benefits and move, waivers merger has the focus to parse all channels of a book effect, also expand both technology and meta operations, and by- data are. (literary-liaisons.com)
Let's take a closer look at the tattooing process and the associated rituals that are an integral part of Maui culture. (hawaiistar.com)

We implemented the app at a university hospital in South Korea to assess the potential for its utilization in a clinical environment by reviewing the usage data among a small group of users and by assessing their feedback and the data related to blood culture sampling. (jmir.org)
Although this app could be used in the clinical setting, improvements in the app functions, environment network, and internal policy of blood culture testing are needed to ensure hospital-wide use. (jmir.org)
It is believed that developing a positive safety culture in healthcare organisations is associated with a beneficial impact on safety outcomes and a lower incidence of clinical and other errors that may result in harm. (biomedcentral.com)

Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
Typical protocols to make gene edited (GE) varieties also use transgenes, even though these may be undesirable in the final crop product. (frontiersin.org)
Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)

These techniques are remarkably adapted to biomedical images when trained on large datasets and powerful computer graphics cards. (brookes.ac.uk)

The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. (benthamscience.com)

3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

Please see our website for a generalized protocol or video on how to use these antibody arrays. (rndsystems.com)

As with the original protocol, total DNA was extracted from the samples and subjected to PCR. (usda.gov)
The PCR products from the soil samples as well as C. beticola pure culture and amended soil controls were run on agarose gels that proved successful amplification of the target pathogen. (usda.gov)
The comparisons confirmed that the excised PCR amplified fragments from amended soil, various field soil samples and the pure culture were all from C. beticola. (usda.gov)
This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples. (jove.com)
In total, 356/644 (55.3%) of all blood culture samples obtained at the MICUs were examined using the app, including 254/356 (71.3%) with blood collection volumes of 5-7 mL and 256/356 (71.9%) with blood collection from the peripheral veins. (jmir.org)

We developed a PCR technique for rapid detection of C. beticola in field soils. (usda.gov)

Understand and discuss the historical and contemporary role of printmaking media in art, design, and culture. (ccri.edu)
Gain familiarity with the tools, media, and techniques inherent to relief processes. (ccri.edu)

One advantage of this technique is its ability to visualize important mitophagy complexes in vivo in tissues with a rare availability or in small sample numbers. (jove.com)

They come in a variety of formats, from techniques to gather focused feedback on a project or piece of writing before revising-like a tuning protocol-to interactive processes like the gallery walk protocol, in which students build on each other's knowledge about a topic or content to promote higher-order thinking through cooperative learning. (ascd.org)
Develop facility with the tools, materials, and techniques inherent to basic printmaking processes. (ccri.edu)
Mobile health apps can be used to resolve problems with blood culture tests, and such apps can hence ensure that point-of-care guidelines are followed and processes are monitored for blood culture tests. (jmir.org)
Safety culture refers to the way that patient safety is considered within an organisation and the structures and processes put in place to support it [ 1 ]. (biomedcentral.com)

Developing a safety culture is associated with beneficial impacts on safety outcomes, but the complex needs of care home residents, coupled with staffing pressures in the sector, pose challenges for positive safety practices to become embedded at the individual and organisational levels. (biomedcentral.com)

This handbook provides both a theoretical and practical guide to techniques currently used in assisted reproduction. (ivf.net)

Overnight culture of peripheral blood was prepared according to standard laboratory protocols. (hindawi.com)

Instead of uploading word docs that can become outdated very quickly, we use protocols.io as a public medium to share our protocols and techniques. (ohsu.edu)
7. Drop the solution evenly over the cell culture medium on the plate. (sigmaaldrich.com)
EV-iPSCs were obtained by differentially ultracentrifuging iPSCs culture medium. (bvsalud.org)

Anatomy10

Organisms10

Diseases7

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment90

Phenomena and Processes21

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture3

Humanities3

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Named Groups1

Health Care43

Geographicals3

Human Pluripotent Stem1

Cells39

Isolation1

Methods and Protocols1

Serum-free1

Assay1

Experiments2

Tissue8

Antibodies1

Researchers6

Vitro5

Adherent2

Optimal2

Genomic1

Molecular Biology2

Intracellular1

Slice Cultures1

Bacterial1

Stain1

Specimens2

Bacteria1

Laboratory Techniques1

Examine1

Step-by-s1

Diagnostic4

Spheroid3

Practice1

Widely2

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Conditions2

Indefinitely1

Actin2

Outlines1

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According to standard1

Medium3