• bacteria
  • While SILAC had been mostly used in studying eukaryotic cells and cell cultures, it had been recently employed in bacteria and its multicellular biofilm in antibiotic tolerance, to differentiate tolerance and sensitive subpopulations. (wikipedia.org)
  • Since 2014, IndieBio, a synthetic biology accelerator in San Francisco, has incubated several cellular agriculture startups, hosting Muufri (making milk from cell culture), Clara Foods (making egg whites from cell culture), Gelzen (making gelatin from bacteria and yeast), Afineur (making cultured coffee beans) and Pembient (biofabricating rhino horn). (wikipedia.org)
  • proliferation
  • This attachment is essential for proliferation - many adherent cell cultures will cease proliferating once they become confluent (i.e., when they completely cover the surface of cell culture vessel), and some will die if they are left in this confluent state for too long. (qiagen.com)
  • It is likely that two different media formulations will be required for each cell type: a proliferation media, for growth, and a differentiation media, for maturation. (wikipedia.org)
  • After more proliferation and longer days in vitro in the presence of EGF, cells eventually became immunoreactive to neurofilament, NSE, and GFAP. (wikipedia.org)
  • Prior to implantation, the NSCs were treated with neurogenin 2 protein to encourage proliferation of the intended inner ear cells. (wikipedia.org)
  • This can be explained in part by their intrinsically low telomerase activity, eradicating the risk of tumorigenesis through unbridled cell proliferation. (wikipedia.org)
  • MSCs
  • Our protocols will guide you through the nuances of culturing iPSCs, NSCs, and MSCs. (thermofisher.com)
  • Originally it was proposed that the MSCs were discarded from the embryo at the end of their life cycle but since the cells remained viable in the amniotic fluid and were able to proliferate in culture this hypothesis was overturned. (wikipedia.org)
  • Comparison of amniotic fluid-derived MSCs to bone-marrow-derived ones showed that the former has a higher expansion potential in culture. (wikipedia.org)
  • However, the cultured amniotic fluid-derived MSCs have a similar phenotype to both adult bone-marrow-derived MSCs and MSCs originating from second trimester fetal tissue. (wikipedia.org)
  • In animals, the MSCs seem to have a unique immunological profile which was observed after their isolation and in vitro culturing. (wikipedia.org)
  • Methods In Molecular Medicine
  • The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (springer.com)
  • Adherent
  • Adherent primary cells are particularly susceptible to contact inhibition, that is, they will stop growing when they have reached confluency. (qiagen.com)
  • Epithelium
  • This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
  • fibroblasts
  • Research is underway to generate stimulus-triggered acquisition of pluripotency (STAP) cells using human tissue: in February 2014, Charles Vacanti and Koji Kojima (Harvard researchers originally involved in the discovery and publication of STAP) claimed to have preliminary results of STAP cells generated from human fibroblasts, but concomitantly cautioned that these preliminary results require further analysis and validation. (wikipedia.org)
  • Hela cells and human fibroblast-derived iPS cells showed high telomerase activity while Muse was at nearly the same level as that in somatic cells such as fibroblasts (these data are shown without running control for the telomerase activity, the comparison is not scientific thought). (wikipedia.org)
  • spontaneously
  • Rodent primary cell cultures form continuous cell lines relatively easily, either spontaneously or following exposure to a mutagenic agent. (qiagen.com)
  • Muse cells are able to generate cells representative of all three germ layers from a single cell both spontaneously and under cytokine induction. (wikipedia.org)
  • Muse cells are shown to home into the damage site and spontaneously differentiate into tissue-compatible cells according to the microenvironment to contribute to tissue regeneration when infused into the blood stream. (wikipedia.org)
  • The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3' cells. (wikipedia.org)
  • proliferate
  • Suspension cells can survive and proliferate without being attached to a substratum. (qiagen.com)
  • Primary cultures are formed from cells that survive the disaggregation process, attach to the cell culture vessel (or survive in suspension), and proliferate. (qiagen.com)
  • These cultures will proliferate for a limited number of cell divisions, after which they will senesce. (qiagen.com)
  • The cells will proliferate for an extended time, but usually the culture will eventually cease dividing, similar to senescent primary cells. (qiagen.com)
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types would adhere to and proliferate upon polycarbonate fibers. (wikipedia.org)
  • undergo
  • In addition, cell lines cultured for extended periods of time can undergo phenotypic and genotypic changes that can lead to discrepancies when comparing results from different laboratories using the same cell line. (qiagen.com)
  • In 2D, the cells must undergo regular trypsinization in order to provide them with sufficient nutrients for normal cell growth. (wikipedia.org)
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • pluripotency
  • The expression 'pattern' of genes related to pluripotency in Muse cells was almost the same as that in ES and iPS cells, while the expression 'level' was much higher in ES and iPS cells and that in Muse cells. (wikipedia.org)
  • advancement
  • Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science. (springer.com)
  • A fundamental missing piece in the advancement of cultured meat is the availability of the appropriate cellular materials. (wikipedia.org)
  • After the creation of the cell lines, efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been the target of most criticisms of cellular agriculture and cultured meat production. (wikipedia.org)
  • Molecular Biology
  • A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). (wikipedia.org)
  • Carol Lynn Curchoe (born in 1979 in Manchester, Connecticut), formerly Carol George, is an American reproductive biologist specializing in Molecular biology, Cell biology and Biotechnology. (wikipedia.org)
  • stem cell th
  • Vemuganti is reported to have done pioneering work in stem cell therapy and was a member of the team led by V. S. Sangwan that developed a protocol for transplanting cultured stem cells for restoring vision in humans. (wikipedia.org)
  • This was subsequently put on clinical trial in 2011, reported to be the largest successful trial of stem cell therapy on humans, till then. (wikipedia.org)
  • Cellular grafts derived from retinal stem cell therapy is an active area of research to treat macular degeneration, retinitis, pigmentosa, and glaucoma. (wikipedia.org)
  • laboratory
  • In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
  • With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. (springer.com)
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • Geeta Kashyap Vemuganti is an Indian ocular pathologist and the head of the department at the Ophthalmic Pathology Service and Stem Cell Laboratory of the L. V. Prasad Eye Institute (LVPEI). (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Later, with the assistance of two philanthropists, they established Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory where he furthered his work. (wikipedia.org)
  • He has also served as an investigator in a number of clinical projects undertaken by Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory. (wikipedia.org)
  • adult
  • In 1992, Brent A. Reynolds and Samuel Weiss attempted to isolate EGF-responsive cells from an adult mouse central nervous system (CNS). (wikipedia.org)
  • Reynolds and Weiss found that, at 21 days, in vitro cultures of spheres and associated cells contained two of the major neurotransmitters of the adult striatum. (wikipedia.org)
  • However, foetal stem cells attained from the amniotic fluid are more stable and more plastic than their adult counterparts making it easier for them to be reprogrammed to a pluripotent state. (wikipedia.org)
  • biology
  • Unforeboding and arcediano Abdías frown his Italianize or accelerated by cell biology alberts 3rd edition calculation reinforcement. (bitradio.tk)
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  • perimeter and cell biology journal articles leggy Leonardo centas moving their celestron sky maps & luminous star finder yokes and defecated mischievously. (bitradio.tk)
  • Cellular agriculture capitalizes on breakthroughs in tissue-engineering, material sciences, bioengineering, and synthetic biology to design new ways of producing existing agricultural products like milk, meat, fragrances, and rhino horn from cells and microorganisms. (wikipedia.org)
  • The technique for producing STAP cells was subsequently studied by Obokata at the Brigham and Women's Hospital (BWH), while she was studying as a post doc under Charles Vacanti, and then at the RIKEN Center for Developmental Biology in Japan. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • With the advent of the field of stem cell biology, the potential of stem cells to form organs in vitro was realized early on with the observation that when stem cells form teratomas or embryoid body, the differentiated cells can organize into different structures resembling those found in multiple tissue types. (wikipedia.org)
  • organs
  • Attempts to create organs in vitro started with one of the first dissociation-reaggregation experiment where Henry Van Peters Wilson demonstrated that mechanically dissociated sponge cells can reaggregate and self-organize to generate a whole organism. (wikipedia.org)
  • The advent of the field of organoids, started with a shift from culturing and differentiating stem cells in 2D media, to 3D media to allow for the development of the complex 3-dimensional structures of organs. (wikipedia.org)
  • mouse embryo
  • A series of experiments, first turning a mouse embryo green by fluorescently tagging STAP cells, then videotaping the transformation of T-cells into pluripotent cells, finally convinced skeptics that the results were real. (wikipedia.org)
  • Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
  • human
  • The proliferative potential of some human finite cell cultures can be extended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). (qiagen.com)
  • Before undertaking any work with human or animal tissue (e.g., to establish a primary cell culture), it is necessary to ensure that the nature of the work conforms to the appropriate medical-ethical and animal-experiment legislation and guidelines. (qiagen.com)
  • Susceptibilities of Mycoplasma fermentans and Mycoplasma hyorhinis to membrane‐active peptides and enrofloxacin in human tissue cell cultures. (currentprotocols.com)
  • Human Cell 14:244‐247. (currentprotocols.com)
  • While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials, it has become clear that most do not. (wikipedia.org)
  • Muse cells are identified as cells positive for SSEA-3+, a well-known marker for undifferentiated human ES cells. (wikipedia.org)
  • showed that human dermal fibroblast-derived Muse cells were efficiently differentiated into melanin-producing functional melanocytes by a cocktail of cytokines. (wikipedia.org)
  • This was shown in human Muse cells infused into animal models with fulminant hepatitis, partial hepatectomy, muscle degeneration, skin injury, stroke and spinal cord injury. (wikipedia.org)
  • In 2013, Madeline Lancaster at the Austrian Academy of Sciences established a protocol for culturing cerebral organoids derived from stem cells that mimic the developing human brain's cellular organization. (wikipedia.org)