• This study presents a technique for repetitive blood sampling in non-anesthetized rats by a single proficient individual. (bvsalud.org)
  • For stereotaxic surgery using rats or other small animals. (ugobasile.com)
  • Using this protocol, centers were formally certified to perform TMS-based neurophysiological measures in this clinical trial of stroke patients. (longdom.org)
  • Much effort has been devoted to the development and testing of semiautomatic and automated tissue segmentation algorithms as well as to the optimization of scanning protocols (2) . (ajnr.org)
  • Bayesian segmentation models provide additional advantages, drawing on empirical information as to the location of tissue classes in stereotaxic space (4) . (ajnr.org)
  • Otherwise functional and molecular imaging techniques may investigate on tumor cellularity and tissue ultrastructure and tumor metabolism and vascularity, providing noninvasive assessment of tumor biology thus improving the care planning process. (radiologykey.com)
  • We used pharmacological, molecular, and immunohistochemical techniques to assess the role of CRF-CRFR1 signaling in CeA in stress-induced hyperalgesia. (nature.com)
  • Whereas optogenetic techniques provide exquisite molecular and temporal specificity for testing the function of specific cell types in sensory acuity, they currently carry inherent and technical limitations that can lead to different levels of opsin expression and activation or suppression strength across individual animals. (jneurosci.org)
  • Current Protocols in Molecular Biology (SUPPL). (cshl.edu)
  • Molecular biology techniques and ELISA were used to examine mitochondrial function, oxidative stress, and the inflammatory response. (biomedcentral.com)
  • Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons. (jove.com)
  • 1 Non-invasive localization techniques such as scalp electroencephalography (EEG), positron emission tomography (PET), magnetic resonance imaging (MRI), ictal single-photon emission computed tomography (SPECT), magnetoencephalography (MEG), and EEG-functional MRI (EEG-fMRI) can help in the identification of the EZ, which sometimes allows some patients to go directly on to resective surgery. (dovepress.com)
  • Intuitive touch screen allows user to easily define complex injection protocols. (ugobasile.com)
  • Modern imaging techniques define the anatomic region infected, the evolution of the disease, and help in better management of these patients. (pediatricneurosciences.com)
  • The stimulation is easily carried out by employing direct current (DC) over the scalp [ 6 ] without any invasive techniques. (biomedcentral.com)
  • In this technique, two electrodes are typically placed on two precise areas of the scalp to complete the flow circuit [ 8 ]. (biomedcentral.com)
  • LORETA developed by Roberto Marquis Pascal in the mid 1990's is a technique based on the best of the inverse solutions which allows quantification and visualization of intracortical activity including paleocortical activity derived from scalp electrodes. (icdrc.org)
  • Subsequently, functional radiosurgery was compared to microsurgical, chemical, and thermal electrode-based techniques to create a lesion. (surgicalneurologyint.com)
  • Importantly, we provide an analytical pipeline containing two free, open source and cross-platform software programs, Occam and post-prOccam, that enable the fully automated analysis of one- and two-photon Ca 2+ imaging datasets from oligodendroglia obtained by either ex vivo or in vivo Ca 2+ imaging techniques. (biorxiv.org)
  • We focus on the used (dried) blood microsampling techniques, collection conditions, stability of the microsamples, sample extraction, analytical methods, method validation, correlation studies with conventional venous blood samples and patient satisfaction. (bvsalud.org)
  • Challenges remain in determining the optimum manner for incorporating these techniques into routine clinical practice, as part of a multimodal protocol. (radiologykey.com)
  • In multi-site clinical trials, this technique has some advantages over other neuroimaging methods owing to its relatively low cost, low personnel and equipment infrastructure requirements, and greater ease in consistently applying technology to collect and analyze data. (longdom.org)
  • Materials and methods: A standardization process was developed to ensure TMS protocol adherence and data quality, and each clinical site was required to successfully complete standardization procedures prior to collecting patient data. (longdom.org)
  • We report diverse ex vivo and in vivo experimental protocols to obtain reproducible Ca 2+ imaging data from oligodendroglia in demyelinated lesions. (biorxiv.org)
  • In conclusion, we demonstrate new techniques capable of interfacing with live neurons, and extendable to in vivo applications. (eneuro.org)
  • Stereotaxic space, a coordinate system applied across scan data to relate data from different patients and different time points in a common 3D space, has been used to advance segmentation techniques. (ajnr.org)
  • To segment the EEG frequencies by using the microstate segmentation technique applied to the frequency domain, and to compare with the results of Herrmann. (icdrc.org)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Conclusion: Although TMS methodological approaches vary, a protocol with standardized procedures was successfully developed and implemented. (longdom.org)
  • Whole-brain volumetric microscopy techniques such as serial two-photon tomography (STPT) can provide detailed information on the roles of neuroinflammation and neuroplasticity throughout the whole brain post-stroke. (frontiersin.org)
  • However conventional imaging techniques, which demonstrate important structural information with a high sensitivity, have limited specificity, especially after previously applied radiation and/or chemotherapy. (radiologykey.com)
  • A major advantage of optogenetics is the ability to genetically-target specific cell populations in heterogeneous brain regions and the technique has been successfully applied to a number of model organisms (invertebrates to nonhuman primates) 4-6 . (jove.com)
  • Optical manipulation techniques, such as optogenetics and caged compounds, enable remote control of neural activity and behavior with fine spatiotemporal resolution. (eneuro.org)
  • Our purpose was to review the role of stereotactic radiosurgery used for functional indications and to discuss future applications and potential techniques. (surgicalneurologyint.com)
  • And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)
  • Radiosurgical techniques were used to create brain lesions without neurophysiologic guidance. (surgicalneurologyint.com)
  • Talairach J, Tournoux P. Co-planar Stereotaxic Atlas of the Human Brain. (icdrc.org)
  • Diffusion tensor imaging (DTI) is an advanced MR technique that describes the movement of water molecules, useful to study microstructural differences among different tumor types and grades. (radiologykey.com)
  • While these techniques result in larger signals which are easier to measure, intracellular or patch-clamp techniques are very challenging in live animals, making extracellular recordings the technique of choice for many investigators. (hindawi.com)
  • Methods: A systematic review of the literature was conducted according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) protocol guidelines. (bvsalud.org)
  • MRS and immunoblotting techniques were used to measure the neurochemical metabolism levels and cholinergic-related proteins, respectively. (biomedcentral.com)
  • 7. Cutting-edge histology/imaging techniques including quantitative bright-field and fluorescence microscopy, confocal microscopy is required. (postdocjobs.com)
  • They will plan and perform MR guided stereotaxic surgeries in nonhuman primates. (nih.gov)
  • In addition to clinically sound techniques, a surgical plan, as described in the ASP, should also consider the availability of personnel to provide anesthetic induction, aseptic preparation of the surgical site, and post- operative care appropriate to the surgical procedure.9 Investigators must assure that the challenges of consecutive surgeries within one work session are adequately addressed. (nih.gov)
  • 8. Performs routine biomedical experimental protocols including immunohistochemistry, immunocytochemistry, ELISA, and neuronal cell culture. (postdocjobs.com)
  • All experimental protocols of this study were approved by the Institutional Ethics Committee of Linyi People's Hospital. (biomedcentral.com)
  • It includes experimental protocols used to evaluate the physiologic effects of immobility. (nih.gov)
  • The consortium is also an effective platform for developing and testing viral techniques for use in nonhuman primates as it allows functional validation of virally delivered constructs in the slice. (nih.gov)
  • Translation of viral techniques from rodents to primates is challenging, but it is an important step towards development of next-generation therapeutics. (nih.gov)
  • In addition, the scientist in this position will have the opportunity to develop cutting edge, next-generation viral techniques for use in non-human primates, gain experience in planning and organizing projects, and mentor fellows. (nih.gov)
  • Mundinano IC, Flecknell PA, Bourne JA (2016) MRI-guided stereotaxic brain surgery in the common marmoset. (nih.gov)
  • Plan coordinates of stereotaxic injections from MRI files. (nih.gov)
  • 5. Performs stereotaxic surgery technique, including cisternal infusions, on rats and mice. (postdocjobs.com)
  • Current techniques to measure LH release and pulsatility require long-term training for mice to adapt to manipulation stress, certain restraint, the presence of the investigator, and working on individual animals, reducing its usefulness for many research questions. (bvsalud.org)
  • This paper presents a technique to remotely activate specific neuronal populations using Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) technology coupled with automated sequential blood sampling in conscious, freely moving, and undisturbed mice. (bvsalud.org)
  • In seven mice, using a 25 μL syringe (Hamilton Co., NV, USA) with 33 g needle (20° beveled point, Hamilton Co., NV, USA) mounted on a stereotaxic arm, 3 μL of QD solution was manually injected over 1 min into the CSF of the cisterna magna. (biomedcentral.com)
  • After shaving of the injury site, mice were transferred into a stereotaxic frame (Just For Mice Stereotaxic Instrument, Stoelting, Solid wood Dale, Illinois) mounted with an electromagnetic controlled impact device (Impact One Stereotaxic Motorized Impactor, Richmond, Illinois). (wwec2012.net)
  • We describe the animal placement on the stereotaxic fixation system, and the procedure for the pineal gland removal and animal recovery from surgery. (nih.gov)
  • We first describe the stereotaxic surgery protocol to deliver adeno-associated virus (AAV) vectors expressing DREADDs to specific neuronal populations. (bvsalud.org)
  • Prior to performing any survival surgery techniques on rodents, an approved Animal Study Proposal (ASP) must be in place with descriptions of the surgical procedures to be performed and the anesthesia/analgesia1,2 that will be used pre-emptively, during and post-procedure. (nih.gov)
  • All survival surgery will be performed by using aseptic procedures, including masks, sterile gloves, sterile instruments, and aseptic techniques. (nih.gov)
  • Our new electrode design provides us stereotaxic accuracy that was not previously available. (whiterose.ac.uk)
  • This innovative technique allows to introduce some electrode needles with small drill holes, performed under local anesthesia, for both recording and therapeutic (electrolysis) functions. (mnemosline.pl)
  • n = 11) were shaved (head and neck) and mounted under general anesthesia (1.5% isoflurane inhalation in O 2 ) onto a stereotaxic frame (Kopf Instruments, CA, USA) in a prone position and a 1 cm skin incision was made from the base of the neck to the tip of the occiput. (biomedcentral.com)
  • PMID- 1853740 TI - [The Akinosi dental anesthesia technique: apropos of 10 clinical cases]. (nih.gov)
  • In this study, the membranous and osseous labyrinths of the wild type mouse inner ear were examined using volumetric data from ultra high-field magnetic resonance imaging (MRI) with gadolinium contrast at 9.4 Tesla and high-resolution micro-computed tomography (µCT) to visualize the scalae and vestibular apparatus, and to establish imaging protocols and parameters for comparative analysis of the normal and mutant mouse inner ear. (openneuroimagingjournal.com)
  • Romodanov A.P., Stepien L., Mempel E. et.al Comparative analysis of results of classic and stereotaxic operstions for epilepsy//In: Symp of stereotactic treatment of epilepsy. (therjn.com)
  • Learn more at giardinolab.org To be successful in this role, the ideal candidate will have: prior neuroscience wet-lab experience, strong organizational and analytical skills, ability to meet deadlines, willingness to learn/master techniques such as measuring & manipulating neural activity in rodent animals, and the ability to attain a high degree of independence in a fast-paced and dynamic research environment. (sanfranrecruiter.com)
  • Recording techniques capable of sampling neural activity at high spatiotemporal resolution have been difficult to scale. (frontiersin.org)
  • Finally, we describe the protocol for clozapine-N-oxide intravenous injection for remote neuronal activation and automated blood collection. (bvsalud.org)
  • Nuevos mecanismos de migración neuronal implicados en el plegamiento de la corteza cerebral. (ub.edu)
  • Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. (elifesciences.org)
  • Therefore, an overarching aim of the Section on Cellular and Cognitive Neurodevelopment is to take a multi-technique approach to explore the development of thalamocortical circuits and their plasticity into adulthood. (nih.gov)
  • The In Vivo Neurobiology Group uses advanced imaging techniques to study the brain circuits involved in voluntary movement and to develop novel methods for diagnosing and treating neurodegenerative disorders such as Parkinson's disease. (nih.gov)
  • Next, we describe the protocol for carotid artery and jugular vein cannulation and postsurgical connection to the CULEX automated blood sampling system. (bvsalud.org)
  • Breast MRI is a highly specialized diagnostic technique that complements clinical assessment and conventional imaging with mammography and ultrasound (US). (medscape.com)
  • In this technique, a tissue is manually sectioned into thin slices on a cryostat before the tissue section is mounted onto a microscope slide, stained and imaged using a microscope. (frontiersin.org)
  • Optical hyperspectral imaging is a non-invasive, whole animal imaging technique based on the spectral analysis of light reflected by the tissue components. (biomedcentral.com)
  • The fusion of ultra high-field MRI and high-resolution µCT imaging techniques were complementary, and provided high-resolution dynamic and static visualization of the complex morphological features of the normal mouse inner ear structures, which may offer a valuable approach for the investigation of cochlear and vestibular abnormalities that are associated with birth defects related to genetic inner ear disorders in humans. (openneuroimagingjournal.com)
  • We conclude that temporary inactivation of the HPC may fail to impair context fear memory because this technique does not sufficiently disrupt the HPC. (scirp.org)
  • The characteristics of the particles and potential implications for their long term fate will be discussed in the context of the techniques applied and the environments in which the particles were found. (ansto.gov.au)
  • The pinealectomy technique consists of the surgical removal of the superficial pineal gland. (nih.gov)
  • tips-only" technique restricts you to using only the sterile working ends of the surgical instruments to manipulate the surgical field. (nih.gov)
  • The Guide states that it is important for research personnel to be appropriately qualified and trained in all procedures to ensure that good surgical technique is practiced. (nih.gov)
  • Overall, we found this technique to be a powerful approach for research on neuroendocrine control. (bvsalud.org)
  • In ongoing research, including the EA1141 Trial, an abbreviated-protocol breast MRI is being investigated as a lower-cost method than traditional full diagnostic protocol breast MRI, while maintaining sensitivity and specificity for breast cancer. (medscape.com)
  • Mouse Polyclonal Gapdh Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)
  • Mouse Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Polyclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)
  • To date, developmental neuroscience studies have relied on permanent lesion techniques to investigate how atypical amygdala development impacts socioemotional behaviors, which may not adequately recapitulate the role of amygdala dysfunction in the manifestation of aberrant behavior. (eneuro.org)
  • Different techniques have been used with similar results (obliteration rate varying between 60% to 86% after 2 years): proton beams, gamma units, heavy-charged particles, and linear accelerators (2-13) . (ajnr.org)
  • However, many of these fabrication protocols use equipment which are not common to biological laboratories, thus limiting researchers to the use of commercial alternatives. (whiterose.ac.uk)
  • Prepare and assist in animal protocol writing. (nih.gov)
  • Experiments were performed in accordance with Office of Laboratory Animal Welfare and National Institutes of Health guidelines under a protocol approved by the Roskamp Institute Institutional Animal Care and Use Committee. (wwec2012.net)
  • This technique allows for programmed automated sampling every 5 min or longer for a given period, coupled with intravenous substance injection at a desired time point or duration. (bvsalud.org)
  • Mammalian brains consist of 10s of millions to 100s of billions of neurons operating at millisecond time scales, of which current recording techniques only capture a tiny fraction. (frontiersin.org)
  • To date, there is no consensus on the optimal follow-up protocol after treatment. (ajnr.org)
  • This thin-section dynamic contrast-enhanced technique formed the foundation of modern breast MRI. (medscape.com)
  • The modern techniques used in Turkey lead to successful tumor treatments. (bookimed.com)
  • 2. Ensure pharmaceuticals are dispensed per protocol, appropriate supplies maintained, secured and disposed of according to established policies and procedures. (postdocjobs.com)
  • offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher. (sanfranrecruiter.com)
  • Assist with orientation and training of new staff or students on lab procedures or techniques. (sanfranrecruiter.com)
  • The group share a large number of techniques including GMP production of Advance Therapy Medicinal Products. (ub.edu)
  • Use of suture materials and patterns or other wound closure techniques that minimize trauma and remain intact. (nih.gov)