PC12 CellsSynaptic VesiclesTransport VesiclesCytoplasmic VesiclesSeminal VesiclesSecretory VesiclesCOP-Coated VesiclesLiposomesExocytosisCell MembraneClathrin-Coated VesiclesMicroscopy, ElectronPhosphatidylcholinesGolgi ApparatusBiological TransportVesicular Transport ProteinsEndocytosisMembrane FusionKineticsPhospholipidsLipid BilayersClathrinEndosomesOrganellesIntracellular MembranesPyridinium CompoundsCell FractionationProtein TransportNerve Tissue ProteinsMicrovilliSNARE ProteinsBiological Transport, ActiveSynaptotagminsCalciumMolecular Sequence DataCarrier ProteinsAmino Acid SequencePresynaptic TerminalsCytoplasmic GranulesMembranes, ArtificialHydrogen-Ion Concentrationrab GTP-Binding ProteinsMembrane LipidsProprotein Convertase 2SynapsinsProtein BindingQa-SNARE ProteinsMicroscopy, FluorescenceVacuolesOrganoidsMicroscopy, ImmunoelectronCattleEndoplasmic ReticulumCoatomer ProteinFluorescent DyesMicroscopy, Electron, TransmissionModels, BiologicalValinomycinMembranesCells, CulturedSubcellular FractionsChromaffin CellsExosomesFreeze FracturingPhosphatidylserinesAdenosine TriphosphateQuaternary Ammonium CompoundsSynaptotagmin INeurotransmitter AgentsCell LineProprotein Convertase 1NeuronsPhosphatidylethanolaminesMicroscopy, ConfocalCell CompartmentationSynaptic TransmissionSynaptic MembranesPhosphatidylglycerolsRabbitsSynapsesTemperatureAdaptor Proteins, Vesicular TransportMutationLysosomesTime FactorsSpectrometry, FluorescenceDimyristoylphosphatidylcholineCytoplasmMicellesGreen Fluorescent Proteinsrab3A GTP-Binding ProteinRecombinant Fusion ProteinsCalcium-Binding ProteinsBrefeldin AAdenosine TriphosphatasesPheochromocytomaCoat Protein Complex IProteolipidsCentrifugation, Density Gradienttrans-Golgi Network